S. Bintara, D. T. Widayati, W. Asmarawati, R. N. Aji
{"title":"The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours","authors":"S. Bintara, D. T. Widayati, W. Asmarawati, R. N. Aji","doi":"10.23960/jipt.v11i1.p34-40","DOIUrl":"https://doi.org/10.23960/jipt.v11i1.p34-40","url":null,"abstract":"In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46397964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Hartati, M. H. Septian, Noor Alif Fitria, R. W. Idayanti, M. Sihite
{"title":"Inulin Extraction from Different Types of Tubers in Magelang District","authors":"L. Hartati, M. H. Septian, Noor Alif Fitria, R. W. Idayanti, M. Sihite","doi":"10.23960/jipt.v11i1.p1-12","DOIUrl":"https://doi.org/10.23960/jipt.v11i1.p1-12","url":null,"abstract":"This study aimed to determine the levels and characteristics of inulin contained in various types of tubers in Magelang district. The samples used were honey sweet potato (Ipomoea batatas L.), purple sweet potato (Ipomoea batatas L.), white sweet potato (Ipomoea batatas L.), gadung (Dioscorea hispida), yam (Dioscorea alata L.), canna (Canna edulis Ker.), and arrowroot (Maranta arundinacea). The variables observed were yield, inulin content, inulin production, water content of the yield, yield solubility, and the degree of acidity (pH) of the yield. The research results obtained from 7 tubers, the highest yield was arrowroot tubers (0.155%). Honey sweet potato gave the lowest yield (0.035%), with the highest inulin content (6.472%), the highest water content (14.385%), and the highest solubility (0.685%). Arrowroot tubers gave the highest yield but the lowest inulin content. The highest inulin production is found in purple sweet potato. The degree of acidity (pH) of the tuber extract was in the range of 4.97-5.96. The conclusion of this study is that different types of tubers produce different content and production of inulin.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43508483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Motility, Viability and Abnormality of Boar Spermatozoa in Various Modified Extenders","authors":"Hermilinda Parera, V. Lenda","doi":"10.23960/jipt.v11i1.p13-33","DOIUrl":"https://doi.org/10.23960/jipt.v11i1.p13-33","url":null,"abstract":"The purpose of this study was to conduct an assessment on the quality of pig semen spermatozoa during storage and determine which diluent material should be used and how long pig semen can be stored. Freshly ejaculated boar semen was obtained from a two-years-old Duroc male and was diluted with Beltsville Thawing Solution (BTS) and Tris Citrate Fructose (TCF) supplemented with 5% egg yolk (KT) and extract of Borassus flabellifer Linn mesocarp (EM) 0.01% and stored at 13°C for up to 4 days. An experimental study with a completely randomized design with four groups and five repeats was applied. Parameters observed were motility, viability and abnormality of spermatozoa. Sperm quality was evaluated by examining motility and abnormality using a phase-contrast microscope with a magnification of 100X, and viability by eosin nigrosine staining. Parametric data were analyzed with ANOVA followed by Duncan test. The results showed that the supplementation of 5% egg yolk in the BTS diluent-based treatment group showed a higher percentage of motility (P<0.05) than without egg yolk, while the TCF-based diluent group showed no differences (P<0.05). Modified-Tris Citrate Fructose diluent supplemented with 5% egg yolk and 0.01% mesocarp extract was able to maintain a significantly higher percentage of viability (67.50±2.0) (P<0.05) than other groups, and had a percentage of abnormalities (9.75±0.957), significantly lower (P<0.05) than other groups. It was concluded that the modification of diluent TCF with 5% egg yolk and mesocarp extract 0.01% provide the best results in maintaining sperm quality with the highest percentage of sperm motility and viability, and the lowest percentage of sperm abnormality compared to the other three diluents. Likewise, all groups of modified-BTS and TCF diluent supplemented with 5% egg yolk and extract of Borassus flabellifer Linn mesocarp in this study were able to maintain motility >40%, viability >45% and abnormalities <20% so they were appropriate for using in the AI procedure.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45941736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and Pathogenicity of Escherichia coli from Cloacal Swabs","authors":"Arie Khoiriyah, S. Sumardi, H. Busman","doi":"10.23960/jipt.v10i3.p323-332","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p323-332","url":null,"abstract":"Colibacillosisis is an infectious disease in poultry caused by pathogenic Escherichia coli (E. coli) as primary or secondary agents. This study were aim to identify and test the pathogenicity ofE. coli isolated from chicken cloacal swabs. The samples used in this study were E. coli isolated from chicken cloacal swabs as many as 42 samples. The first stage in this research was subculturing E.coli in Eosin Methylene Blue Agar (EMBA) medium, then identified by Gram stain and biochemistry with Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA) and Methyl Red Voges-Proskauer (MRVP). Pathogenicity of E. coli was tested by culturing in Blood Agar to see the results of hemolysin production and on Sorbitol-MacConkey Agar (SMAC) to see the ability of Sorbitol fermentation. The results obtained were 12 isolates was pathogenic E. coli looked from the results of the pathogenicity test in the Blood Agar and SMAC Agar.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46749124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improvement of Water Hyacinth (Eichhornia crassipes) Quality as Animal Feed through Fermentation Using Rumen Microbes with the Addition of Molasses","authors":"Desi Susanti, Suci Rahmi","doi":"10.23960/jipt.v10i3.p313-322","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p313-322","url":null,"abstract":"One of the potential alternative feed ingredients as animal feed is water hyacinth. However, the low quality and palatability cause water hyacinth not being liked by ruminants. One way to improve the quality of water hyacinth is using controlled technology involving rumen microbes. The purpose of the study was to determine the level of addition of molasses and fermentation time in improving the quality of water hyacinth using rumen microbes through the test parameters for crude protein and crude fiber content. This study used a completely randomized design (CRD) with factorial patterns with molasses levels, namely M1 = 5%, M2 = 10%, M3 = 15%, and fermentation time consisting of T1 = 7 days, T2 = 10 days, T3 = 13 days . Each experimental design was repeated 3 times to form 27 experimental units. Data were analyzed using variance, if there was a significant treatment effect (P<0.05), followed by the HSD test on the average treatment. The results showed that the highest crude protein content was 14.23% in the M2T3 treatment (10% molasses level with 13 days of fermentation time) (P<0.05) while the lowest crude fiber was 14.55% in the M3T2 treatment (15% molasses level with a dangerous time) 10 days). This treatment was significantly different (P<0.05) from the other treatments. The results of the study concluded that the addition of 10% molasses for 13 days of brewing time could increase the crude protein content in water hyacinth fermented using rumen-filled microbes.and the lowest decrease in crude fiber was at 15% molasses level with 10 days of fermentation.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44959875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Fathul, E. Erwanto, A. K. Wijaya, A. Dakhlan, F. T. Farda, Etha Azizah Hasiib
{"title":"Physical, Chemical Quality and Constituent of Amoniated Cassava Peel with Various Levels of Urea Addition","authors":"F. Fathul, E. Erwanto, A. K. Wijaya, A. Dakhlan, F. T. Farda, Etha Azizah Hasiib","doi":"10.23960/jipt.v10i3.p300-312","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p300-312","url":null,"abstract":"Cassava peel is a waste from the cassava plant which has the potential to be used as ruminant animal feed. Processing of cassava skin with ammonia needs to be done to improve the quality of cassava skin. The research was conducted to determine the physical and chemical quality of cassava peels after amoniation. The study was analyzed using a completely randomized design (CRD) and the significant results were further tested using Duncan's multiple range test (DMRT). The treatment was T1 (cassava peels + 0kg urea); T2 (cassava peels + 0.02kg urea) ; T3 (Cassava peels + 0.04kg urea). Each treatment consisted of 5 repetitions. The organoleptic test was carried out with 30 test participants. The physical tests observed were color, texture and aroma. Chemical quality was determined through proximate analysis and Scanning Electron Microscope-Energy Dispersive X-Ray (SEM-EDX). Variables in the chemical quality test included dry matter, ash, crude protein, ether extract and crude fiber of cassava peels. The results showed that ammoniation of cassava peels affected the physical and chemical qualities of cassava peels (P<0.05). Ammoniation of cassava peel increases protein content up to 23.01% (T3) and reduces crude fiber up to 7.92% (T2). Increasing the level of urea can increase the process of degradation of starch structure in cassava peels.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45278793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Utilization of Duck Feet Gelatin with the Additional Glycerol as A Plasticizer on the Physical Properties of Edible Film","authors":"Hana Aulia Andiati, Jajang Gumilar, E. Wulandari","doi":"10.23960/jipt.v10i3.p289-299","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p289-299","url":null,"abstract":"The packaging that is widely used is plastic packaging, but this material has a weakness that is difficult to decompose and isn’t biodegradable, therefore edible film can be an alternative primary packaging for food products. The material used consists of gelatine made from duck feet with the addition of various concentrations of glycerol. Duck feet is an underutilized portion of the animal's anatomy, yet they contain collagen, which has the potential to be the raw material for gelatine and edible films. The study was undertaken utilizing duck feet as a source of raw material, which was subsequently processed into gelatine for use in the production of edible films using different glycerol treatments. The treatments comprised of glycerol concentrations of P1 = 10%, P2 = 20%, P3 = 30%, P4 = 40%, P5 = 50%, and P6 = 60%. The findings indicated that the application of glycerol significantly affected the films' solubility, water absorption, and tensile strength (P<0.05), but didn’t significantly affect the elongation of edible films. (P>0.05). The optimal concentration of glycerol for edible film in this study was obtained by using 20% glycerol, with a physical characteristic value of 40.25% solubility, 24.23% water absorption, tensile strength is 0.57 MPa, and elongation is 90%.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48590376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ibnul Abror Musthofa, Budi Ayuningsih, I. Hernaman
{"title":"Performance of Female Thin Tail Sheep Fed Ration Containing Fermented Shrimp Waste by EM-4","authors":"Ibnul Abror Musthofa, Budi Ayuningsih, I. Hernaman","doi":"10.23960/jipt.v10i3.p277-288","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p277-288","url":null,"abstract":"The use of shrimp waste as ruminant feed is constrained by the presence of chitin which is difficult to digest. This study aimed to determine the use of shrimp waste fermented with EM-4 as additional feed for female Thin Tailed sheep. A total of 18 female Thin-Tailed sheep with a body weight of 10.35±0.88 kg were allocated to 3 ration treatments containing fermented shrimp waste with EM-4, namely 5%, 7.5% and 10%. The sheep were kept for 90 days at CV. Bungah Tani Djaya's Livestock to measure its performance. The results showed that the use of fermented shrimp waste up to 10% did not interfere with the performance of femaleThin-Tailed sheep with the average dry matter consumption, crude protein consumption, total digestible nutrient (TDN) consumption, daily body weight gain, and feed conversion were 340,29 g/head/day, 31.68 g/head/day, 195.63 g/head/day, 61.80 g/head/day, and 6.18 respectively. The results of the study concluded that the use of shrimp waste fermented with EM-4 up to 10% can be used as additional feed for female Thin-Tailed sheep.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46244783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anaerobic Fermentation of Kepok Banana (Musa Paradisiaca) Peels with Different Levels of Tapioca for Reducing Their Fiber Components","authors":"T. N. I. Koni, Lodofikus Tori, T. A. Y. Foenay","doi":"10.23960/jipt.v10i3.p254-263","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p254-263","url":null,"abstract":"Banana peel is restricted to use as feedstuffs because it has a high crude fiber content. The reduction of crude fiber can be done by anaerobic fermentation such as silage making. Soluble carbohydrates are needed as an energy source for microorganisms in ensilage process. Tapioca is one of soluble carbohydrates that can be used to improve silage quality. The objective of this study was to evaluate effect of tapioca levels on the fiber fraction of kepok banana peel silage (Musa paradisiaca). A completely randomized design was used in this study, with 4 treatments and 5 replications, namely T0: without tapioca, T5: 5% tapioca, T10: 10% tapioca, T15: 15% tapioca. Variables observed were the content of NDF (%), ADF (%), hemicellulose (%), cellulose (%), and lignin (%).Collected data was analyzed by analysis of variance and if it had a significant effect, it was continued with Duncan's multiple range test. The results of analysis of variance showed that the level of tapioca had a highly significant effect (P<0.01) on NDF, ADF, hemicellulose, cellulose, and lignin. DMRT showed that the lowest NDF content was at T15 with an average of 48.06%, the lowest ADF was found at T15 with an average of 20.20%, the lowest hemicellulose was found at T15 with an average of 27.46%, the lowest cellulose was found at T10 with an average of 7.98% and the lowest lignin against T15 with an average of 12.09%. In conclusions, 1) Different levels of tapioca was can reduce ADF, NDF, hemicellulose, cellulose, and lignin in kepok banana peel silage, 2) The use of 10% tapioca reduced the highest kepok banana peel silage fiber fraction.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47387988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Afriani, E. Purwati, J. Hellyward, Jaswandi Jaswandi, M. Mundana, A. Farhana, A. Rastosari
{"title":"Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle","authors":"T. Afriani, E. Purwati, J. Hellyward, Jaswandi Jaswandi, M. Mundana, A. Farhana, A. Rastosari","doi":"10.23960/jipt.v10i3.p264-276","DOIUrl":"https://doi.org/10.23960/jipt.v10i3.p264-276","url":null,"abstract":"The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.","PeriodicalId":17809,"journal":{"name":"JURNAL ILMIAH PETERNAKAN TERPADU","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49369416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}