Jurnal Tumbuhan Obat Indonesia最新文献

{"title":"EKSPLORASI SPESIES TUMBUHAN BERKHASIAT OBAT BERBASIS PENGETAHUAN LOKAL DI KABUPATEN PIDIE","authors":"Saudah Saudah, Vera Viena, Ernilasari Ernilasari","doi":"10.22435/jtoi.v12i2.952","DOIUrl":"https://doi.org/10.22435/jtoi.v12i2.952","url":null,"abstract":"ABSTRACT \u0000Presently the community tends to avoid the use of modern medicine and turn to nature (back to nuture) with traditional medicine using medicinal plants. The exploration of medicinal plant used in traditional medicine in Pidie District aims to explore the potential of plant species used, record the plant parts used, how to process and to use the plants and how to obtain them from the nature habitat. The method used for data collection is exploratory surveys and Participatory Rural Appraisal (PRA) methods. The data obtained were analyzed descriptively and showed in the form of tables and images. The results of the study found 106 types of plants used in traditional medicine that were spread into 67 plant families. The most widely used of medicinal plant species were from the Zingiberaceae family. The most widely used plant part is the leaf part. The method of medicinal processing is done by boiling (decoction), the results of the ingredients are used as oral administration by drinks. Generally, the plants used for medicine by the people of Pidie Distric are wild plants, and 68% of which grow from home gardens and  fields. \u0000ABSTRAK \u0000Kecenderungan masyarakat saat ini mulai menolak penggunaan obat moderen dan beralih ke alam (back to nuture) dengan pengobatan tradisional menggunakan tumbuhan obat. Ekspolarsi jenis tumbuhan obat yang digunakan dalam pengobatan tradisonal di Kabupaten Pidie bertujuan untuk menggali potensi jenis tumbuhan yang digunakan, mendata bagian yang digunakan, cara pengolahan dan penggunaan tumbuhan serta cara mendapatkannya dari alam. Metode yang dilakukan dalam pengumpulan data adalah survey eksploratif dan Participatory Rural Appraisal (PRA). Data yang diperoleh dianalisa secara deskriptif dan ditampilkan dalam bentuk tabel dan gambar. Hasil penelitian didapatkan 106 spesies tumbuhan obat yang digunakan dalam pengobatan tradisional yang berasal dari 67 famili.  Spesies tumbuhan obat yang paling banyak digunakan di wilayah Pidie berasal dari famili Zingiberaceae. Bagian tumbuhan obat yang paling banyak digunakan adalah bagian daun. Cara pengolahan tumbuhan obat secara umum dilakukan dengan perebusan, hasil ramuan digunakan dalam bentuk minuman. Secara umum  tumbuhan yang digunakan untuk obat oleh masyarakat Kabupaten Pidie adalah jenis tumbuhan liar, dan  sebanyak 68% tumbuh dari pekarangan rumah maupun kebun atau ladang. \u0000 ","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74052243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UJI PENANGKAPAN RADIKAL 2,2-difenil-1-pikrilhidrazil (DPPH) OLEH EKSTRAK ETANOL BUNGA KECOMBRANG (Nicolaia speciosa (Bl.) Horan) DAN BUAH TALOK (M. calabura L. )","authors":"Tatang Irianti, Hari Purnomo, Kuswandi Kuswandi, Sindu Nuranto, Damiana Nitya Kanistri, Yosi Bayu Murti, Sofa Farida","doi":"10.22435/jtoi.v12i1.1582","DOIUrl":"https://doi.org/10.22435/jtoi.v12i1.1582","url":null,"abstract":"ABSTRACT \u0000The process of excessive free radicals  in our body plays a major part in the development of chronic and degenerative illness such as cancer, autoimmune disorders, aging, cataract, rheumatoid arthritis, cardiovascular and neurodegenerative diseases. The recently study reported that free radical and oxidants play a dual role as both toxic and beneficial compounds, since they can be either harmful or helpful to the body. Therefore, natural antioxidants can be explored to prevent degenerative diseases and in the present paper we have investigated antioxidant activity of extracts from Nicolaia speciose (Bl.) Horan flower and Muntingia calabura L.  fruit for its free radical scavenging activity using 2,2-diphenyl, 1-picryl hydrazil (DPPH) radical scavenging activity. The results revealed that both ethyl acetate fractions of N.  speciaose and M. calabura have the highest antioxidant activitiy with IC50 29.81 and 14.48 µg/ml respectively.   The antioxidant activity of both ethanolic extracts were more potent than hexan and water fractions with IC50 39.27 and 137.20 µg/ml respectively. The investigation of IC50 values indicated that the antioxidant activity show moderate to very active. Active compounds were identified using thin layer chromatography with FeCl3, AlCl3, 2,4-DNPH and anisaldehide-H2SO4 spray reagents. Chromatogram of ethylacetate fraction showed that spots on the hRf 12 and 56 were thought to contain phenolic compounds with a carbonyl group, while the hRf 37 was suspected flavone compounds with 3-OH group and the hRf 50 was alleged the ortho-dihydroxy flavone or ortho hydroxy and free carbonyl. Chromatogram of ethyl acetate of talok fruits fraction showed that spots on the hRf 19.31 and 44 were suspected flavone compounds with ortho-hydroxy-carbonyl group and or ortho-hydroxy. \u0000ABSTRAK \u0000Radikal bebas berlebih dalam tubuh dapat memicu tumbuhnya sel kanker, penyumbatan pembuluh jantung, kerusakan oksidatif otak dan penuaan dini. Hal ini menyebabkan penelitian tentang potensi antioksidan terus mengalami peningkatan, terutama antioksidan alami dari tanaman. Bunga kecombrang (Nicolaia speciosa (BI.) Horan) dan buah talok (Muntingia Calabura L.) dilaporkan aktif sebagai antioksidan dan mengandung senyawa aktif dengan tingkat kepolaran yang berbeda. Oleh karena itu, penelitian ini bertujuan untuk menguji aktivitas dengan penangkapan radikal bebas fraksi-fraksi ekstrak bunga kecombrang dan buah talok serta karakterisasi golongan senyawa aktifnya. Hasil fraksinasi ekstrak diuji dengan larutan DPPH 0,4 mM dan diukur dengan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Hasil absorbansi sampel dan kontrol diolah untuk mendapatkan persen penangkapan radikal DPPH dan aktivitas antioksidan dievaluasi melalui nilai IC50. Senyawa aktif diidentifikasi menggunakan kromatografi lapis tipis dengan penampak bercak FeCl3, AlCl3, 2,4-DNPH dan anisaldehid-H2SO4.  Fraksi-fraksi ekstrak bunga kecombrang dan buah talok menunjukkan tingkat kekuatan ","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74810305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION, CLIMBING AND COITUS FREQUENCIES IN MALE SPRAGUE-DAWLEY RATS","authors":"N. Rahmawati, I. Sholikhah","doi":"10.22435/jtoi.v12i1.1807","DOIUrl":"https://doi.org/10.22435/jtoi.v12i1.1807","url":null,"abstract":"ABSTRACT \u0000Talinum paniculatum (som Java) reported to have many pharmacological activities such as increasing spermatozoa quality, cell regenerations, fertility, and antibacterial effect both empirically and scientifically. This study aimed to prove the combination infusion effects of som Java, gotu kola, and temulawak on introduction, climbing, and coitus levels as well as testosterone levels in male Sprague Dawley (SD) rats. SD rats were divided into 2 groups, control group received aquadest, treatment group received formula a mixture of som java Talinum paniculatum) root, gotu kola (Centella asiatica) herb, and Javanese turmeric (Curcuma xanthorrhiza) rhizome with the dose of 491,2 mg/200 g BW for 7 consecutive days. Artificial estrus of female SD rats were induced by administering estradiol valerat 2 mg/200 g BW 48 hours before observation. The determined parameters were levels of testosterone as well as introduction, climbing and coitus frequencies. Administration of estradiol valerat led to artificial estrus induction of female SD rats. The administration of the combination infusion dose 491.2 mg/200 g BW significantly increased testosterone level as well as introduction and climbing frequencies of male SD rats as of 27.77%; 86.39% and 69.17% respectively. \u0000ABSTRAK \u0000Som jawa (Talinum paniculatum) secara empiris dan ilmiah terbukti telah banyak dimanfaatkan oleh masyarakat untuk mengatasi berbagai masalah kesehatan diantaranya untuk meningkatkan kualitas dan kuantitas spermatozoa, regenerasi sel, fertilitas, dan sebagai antibakteri. Penelitian ini dilakukan untuk membuktikan efek peningkatan kadar hormon testosteron serta frekuensi introduction, climbing, dan coitus tikus jantan SD dari pengaruh infusa kombinasi som jawa, pegagan, dan temulawak. Hewan uji tikus galur SD dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan akuades, sedangkan kelompok perlakuan diberikan infusa ramuan akar som jawa, herba pegagan, dan rimpang temulawak dosis 491,2 mg/200 g bb selama 7 hari berturut-turut. Induksi estrus artifisial tikus betina dengan pemberian estradiol valerat 2 mg/200 g bb dilakukan 48 jam sebelum pengamatan. Parameter yang diukur meliputi kadar testosterone, frekuensi introduction, climbing, dan coitus. Dibandingkan dengan kelompok kontrol, pemberian infusa ramuan tidak berefek pada frekunsi coitus, namun mampu meningkatkan kadar testosteron serta frekuensi introduction dan climbing tikus jantan sebesar 27,77; 86,39 dan 69,17%. \u0000  \u0000  \u0000  \u0000  \u0000 ","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85426859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KARAKTERISTIK MUTU KAPSUL RAMUAN KEBUGARAN UNTUK SAINTIFIKASI JAMU","authors":"Sofa Farida, Tofan Aries Mana, T. F. Dewi","doi":"10.22435/jtoi.v12i1.1078","DOIUrl":"https://doi.org/10.22435/jtoi.v12i1.1078","url":null,"abstract":"ABSTRACT \u0000\"Ramuan kebugaran\" or Physical Improvement Herb is one results of Saintifikasi Jamu research that has been proven its efficacy through clinical research. The concoction herbs consists of Javanese turmeric rhizome, turmeric rhizome, meniran herbs and it was prepared as decoction. Herbs in the capsule dosage form is considered easier to consume and more appropriate in determining the dosage. This research aimed to study the right filler for capsule dosage form of “Ekstrak Ramuan Jamu Kebugaran” (ERJK) for fulfilling the quality requirements. The ERJK extraction is obtained by infundation method, the extract filtrate was added with fillers, dried, and powdered. The filler used was avicel 102 (ERJK-1), lactose (ERJK-2) and amylum (ERJK-3). The powders were tested for flowability, tapping indexes, and microorganism contamination tests. The capsules dosage form were tested for weight uniformity and disintegration time. The results of the ERJK-1, ERJK-2 and ERJK-3 quality parameters test showed the flow times were respectively 6.3, 7.8 and 5.8 seconds; the tapping indexes were 14.3, 15.35 and 13.0%; yeast count were 1.2, 2.85 and 2.95 col/g; the total plate count were 2.0; 1.0 and 1.0 col/g; capsul weight uniformity  (mg) were 677.04±5.30 (CV: 0.78%); 663.95±8.54 (CV: 1.28%) and 678.38±4.93 (CV: 0.72%); capsul disintegration time (minute) were 25.60, 27.89 and 26.47. The evaluation of quality parameters shows that avicel 102 is the better filler of ERJK compared to lactose and amylum. \u0000ABSTRAK \u0000Ramuan kebugaran merupakan salah satu jamu hasil riset Saintifikasi Jamu yang efektifitas khasiatnya telah terbukti melalui uji klinik. Ramuan tersebut berupa simplisia rimpang temulawak, rimpang kunyit dan herba meniran dan disiapkan melalui proses perebusan. Ramuan dalam bentuk sediaan kapsul dipandang lebih mudah dikonsumsi dan lebih tepat dalam penentuan dosis. Tujuan penelitian ini adalah mengkaji bahan pengisi yang tepat untuk kapsul ekstrak ramuan jamu kebugaran (ERJK) yang memenuhi persyaratan kualitas. Ekstraksi ERJK dengan metode infundasi, filtrat ekstrak ditambahkan bahan pengisi, dikeringkan kemudian diserbuk. Pengisi yang digunakan adalah avicel 102 (ERJK-1), laktosa (ERJK-2) dan amilum (ERJK-3). Serbuk sampel dilakukan uji sifat alir, indeks pengetapan, serta uji cemaran mikroorganisme. Sediaan kapsul diuji keseragaman bobot dan waktu hancur. Hasil uji parameter kualitas ERJK-1, ERJK-2 dan ERJK-3 menunjukkan waktu alir berturut-turut 6,3, 7,8, dan 5,8 detik; indeks pengetapan berturut-turut 14,3, 15,4 dan 13,0%; angka Jamur berturut-turut 1,2, 2,85 dan 2,95 kol/g; angka lempeng total berturut-turut 2,0; 1,0 dan1,0 kol/g; keseragaman bobot kapsul (mg) berturut-turut 677,04±5,30 (CV: 0,78%), 663,95±8,54 (CV: 1,28%) dan 678,38±4,93 (CV: 0,72%); uji waktu hancur kapsul (menit) berturut-turut 25,60, 27,89 dan 26,47. Evaluasi parameter kualitas menunjukkan avicel 102 paling baik digunakan sebagai pengisi ERJK dibandingkan laktosa dan amilum. \u0000 ","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90459281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PROFIL METABOLIT BERBAGAI EKSTRAK DAUN Chrysophyllum cainito L. MENGGUNAKAN UPLC-QTOF-MS/MS","authors":"Burhan Ma’arif, Roihatul Muti’ah","doi":"10.22435/jtoi.v12i1.672","DOIUrl":"https://doi.org/10.22435/jtoi.v12i1.672","url":null,"abstract":"ABSTRACT \u0000Chrysophyllum cainito L. is a plant which empirically used as traditional medicine. The pharmacological effect of C. cainito is caused by secondary metabolite activity contain in the leaves. The aim of this research was to know the metabolites profile in n-hexane extract, ethyl acetate extract, and methanol extract of C. cainito leaves using UPLC-QToF-MS/MS. Dried powder of C. cainito leaves was extracted with n-hexane, ethyl acetate, and methanol with gradual extraction using Ultrasonic Assisted Extraction (UAE). Each extract was prepared with methanol and DCM solvent then injected 5 µl into UPLC-QToF-MS/MS and analyzed with Masslynx 4.1 softwares and Chemspider. The result showed that there were 28 compounds from n-hexane extract with diethyltoluamide as major compound, 47 compounds from ethyl acetate extract with loliolide as major compound, and 34 compounds from methanol extract with eplerenone as major compound. Based on literature study, there were also several compounds that likely having activity as phytoestrogens. \u0000Keyword: Metabolite profiling, chrysophyllum cainito L., UPLC QToF-MS/MS \u0000  \u0000  \u0000ABSTRAK \u0000Chrysophyllum cainito L. merupakan tumbuhan yang secara empiris digunakan sebagai obat tradisional. Efek farmakologi tersebut disebabkan adanya aktivitas dari berbagai senyawa metabolit sekunder yang terkandung dalam daun C. cainito. Tujuan dari penelitian ini untuk mengetahui profil metabolit ekstrak n-heksana, ekstrak etil asetat, dan ekstrak metanol daun C. cainito menggunakan UPLC-QToF-MS/MS. Serbuk kering daun C. cainito diekstraksi secara bertingkat menggunakan n-heksana, etil asetat, dan metanol dengan metode UAE. Masing-masing ekstrak dipreparasi dengan metanol dan DCM lalu diinjeksikan sebanyak 5 µl ke dalam UPLC-QToF-MS/MS, kemudian dianalisis dengan software Masslynx 4.1 dan Chemspider. Hasil menunjukkan profil metabolit dari masing-masing ekstrak daun C. cainito, yaitu ekstrak n-heksana dengan 28 senyawa dan diethyltoluamide sebagai senyawa mayor, 47 senyawa terkandung dalam ekstrak etil asetat dengan senyawa mayor loliolide, dan 34 senyawa terkandung dalam ekstrak metanol dengan senyawa mayor yaitu eplerenone. Dari studi literatur diketahui terdapat beberapa senyawa yang memiliki aktivitas sebagai fitoestrogen. \u0000Kata Kunci: Metabolite profiling, Chrysophyllum cainito L., UPLC QToF-MS/MS","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84318644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTITHROMBOCYTOPENIA ACTIVITY OF PEANUT SHELL (Arachis hypogea L.) EXTRACT AND INFUSA ON HEPARIN INDUCED BALB/C MICE","authors":"Risha Fillah Fithria, R. Wulandari, D. Hidayati, Pia Annisa, Nilam Eka Putri","doi":"10.22435/jtoi.v12i1.1266","DOIUrl":"https://doi.org/10.22435/jtoi.v12i1.1266","url":null,"abstract":"ABSTRACT \u0000Certain group of people empirically use peanut shell stew in the treatment of thrombocytopenia due to dengue fever. The active compounds suspected to play a role in the activity of thrombocytopenia from peanut shell are flavonoids. Flavonoids can be extracted using heat and cold methods. This study aims to determine the antithrombocytopenia activity of peanut shell extract and infusa on heparin induced Balb/C mice. This study is an experimental research with randomized matched pre and postest control group design. Peanut shell extraction was done in a cool way by maseration using 70% ethanol solvent, and hot way by infundation using aquadest solvent. Forty-eight Balb/C mice were divided into 8 groups consisting of control group CMC Na 0.5 mL/20gBW/day, control group aquadest 0,5mL/20gBW/day, 3 groups of peanut shell ethanol extract (0.019, 0.038, and 0.076) g/20gBW/day, and 3 groups of peanut shell infusa (0.026, 0.052; 0.104) g/20gBW/day. All treatments were given orally. The decrease of platelet count in Balb/C mice using 26 UI/20gBW subcutan heparin induction. The measurement of the platelet count is performed by taking blood samples in the lateral veins of the tail. Platelet counts data before and after treatment was tested with dependent T-test. Differences in platelet increases across the groups were tested by 2 way anova and continued with a LSD test with 95% confidence level. The results showed that peanut shell ethanol extract had better antitrombocytopenia activity than peanut shell infusa.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79318972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUASI DAN IDENTIFIKASI GOLONGAN SENYAWA POTENSIAL ANTIBAKTERI PADA DAUN DAN KULIT BATANG MIMBA (Azhadirachta indica A. Juss) TERHADAP Escherichia coli","authors":"Arif Setiawansyah, A. Hakim, D. G. Wirasisya","doi":"10.22435/JTOI.V11I2.1003","DOIUrl":"https://doi.org/10.22435/JTOI.V11I2.1003","url":null,"abstract":"ABSTRACT \u0000Neem (Azadirachta indica A.Juss) is a plant that potentially developed for antibacterial agent for both the leaves and barks. The aims of this study were to compare the effectiveness of the antibacterial activity of neem leav es and stem barks extract and to identify the antibacterial compounds of the most active fractions. The extraction method was done using sonication method. Antibacterial activity was evaluated using wells solid diffusion method and TLC-Bioautography. Extract fractionation was conducted using liquid-liquid partitioning method. The chemical compounds of extracts and fractions were analyzed using TLC and GCMS. The result of sonication extraction obtained neem leaves oil (12,02%), leaves crude extract (4,3%) and stem barks crude extract (16,85%). The major chemical constituents of GCMS analysis are 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS) Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-Dyhidrobenzofuran (2,69%), alpha-D-methylglucopyranoside (4,54%), palmitic acid (2,92%), Arabino-hex-1-enitol, 1,5-Anhydro-2-deoxy-(CAS) glucal (31,69%). Phytochemical screening of neem leaves oil, leaves and barks crude extract revealed the presence of alkaloids, flavonoids, phenols, saponins, triterpenoids, steroids and sterols. Antibacterial test results showed neem leaves oil was more effective than leaves and stem barks crude extract against Escherichia coli. The n-hexane fraction showed higher antibacterial activity than ethyl acetate fraction and ethanol fraction. Phytochemical screening of n-hexane fraction showed the presence of triterpenoids, steroids, sterols and phenols. \u0000  \u0000Mimba (Azadirachta indica A.Juss) merupakan tanaman yang berpotensi dikembangkan sebagai antibakteri baik bagian daun maupun kulit batang. Penelitian ini bertujuan untuk membandingkan efektivitas antibakteri ekstrak daun dan kulit batang mimba terhadap Escherichia coli dan untuk mengidentifikasi golongan senyawa potensial antibakteri pada fraksi teraktif. Pengujian aktivitas antibakteri dilakukan dengan metode difusi padat menggunakan sumuran dan KLT-Bioautografi. Fraksinasi ekstrak dilakukan dengan metode partisi. Komponen kimia ekstrak dan fraksi dianalisis menggunakan KLT dan GCMS. Hasil ekstraksi sonikasi diperoleh minyak daun (12,02%), ekstrak kasar daun (4,3%) dan ekstrak kasar kulit batang (16,85%). Skrining fitokimia menunjukkan minyak daun, ekstrak kasar daun dan ekstrak kulit batang mimba mengandung senyawa alkaloid, flavonoid, fenolik, saponin, triterpenoid, steroid dan sterol. Komponen kimia mayor hasil analisis GCMS minyak daun mimba adalah 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS)Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-dyhidrobenzofuran (2,69%), Alpha-d-methylglucopyranoside (4,54%), Asam Palmitat (2,92%), Arabino-hex-1-enitol, 1,5-anhydro-2-deoxy-(CAS)glucal (31,69%). Hasil ","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74820118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AKTIFITAS RAMUAN JAMU CABE JAWA, DAUN SENDOK DAN SELEDRI TERHADAP TIKUS HIPERURISEMIA","authors":"Ulfa Fitriani, Enggar Wijayanti, Zuraida Zulkarnain","doi":"10.22435/JTOI.V11I2.535","DOIUrl":"https://doi.org/10.22435/JTOI.V11I2.535","url":null,"abstract":"Hiperurisemia merupakan suatu keadaan dimana terjadi peningkatan kadar asam urat darah melebihi nilai normal. Cabe jawa (Piper retrofractum), daun sendok (Plantago major), dan seledri (Avium graveolens) diketahui memiliki khasiat sebagai penurun asam urat darah. Penggunaan kombinasi ketiga tanaman obat tersebut sebagai anti hiperurisemia belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk menentukan khasiat ketiga tanaman tersebut dalam satu ramuan jamu. Jenis penelitian ini adalah eksperimental murni dengan pre and post test controlled design. Hasil intervensi selama 7 hari pada 30 ekor tikus putih jantan yang terbagi dalam 5 kelompok yakni kelompok kontrol (tanpa perlakuan), kelompok kontrol positif (allopurinol), kelompok ramuan jamu dengan 3 macam dosis masing-masing 0,2 g, 0,4 g dan 0,8 g, menunjukan bahwa kadar asam urat darah pada kelompok perlakuan mengalami penurunan. Pemberian ramuan jamu anti hiperurisemia dengan dosis 0,4 g mampu menurunkan kadar asam urat darah dengan rerata paling tinggi. Ramuan jamu  yang terdiri dari cabe jawa, daun sendok dan seledri mampu menurunkan kadar asam urat darah pada tikus. \u0000  \u0000Kata kunci: Cabe jawa, daun sendok, seledri, hiperurisemia","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82606230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDI ETNOFARMAKOLOGI TUMBUHAN OBAT YANG DIGUNAKAN OLEH PENYEHAT TRADISIONAL UNTUK MENGATASI DIARE DI SULAWESI SELATAN","authors":"Fanie Indrian Mustofa, Nuning Rahmawati","doi":"10.22435/jtoi.v11i2.580","DOIUrl":"https://doi.org/10.22435/jtoi.v11i2.580","url":null,"abstract":"ABSTRACT \u0000Medicinal plants for health effort and diseases treatment has been used by ethnic groups in South Sulawesi for years. One of them is for diarrhea treatment. South Sulawesi is one of the top five provinces with the highest incidence and period prevalence of diarrhea in Indonesia. The purpose of this study was to investigate the species of medicinal plants used by traditional healer in South Sulawesi for anti-diarrhea and their scientific evidence. The data was obtained from the exploration of local ethnomedicin knowledge and medicinal plant based on community in Indonesia in 2012-2015 and 2017. The data was analyzed to find out the fidelity level, used value (UV), choice value (CV), and factor of informant’s consensus (FIc). The result reported  information about thirty medicinal plants used for diarrhea treatment, including the plants name, part used, and preparation method that obtained from 48 traditional healer of 19 ethnic groups in South Sulawesi. The fidelity level were 41,67% for Psidium guajava, 8,33% for Mangifera sp, 6,25% for Curcuma domestica and C. Zedoaria, 4,17% for Allium cepa, Anacardium officinale, Syzigium cumini, and C.xanthorrhiza. The highest UV and CV were 0,42 and 13,84 for Psidium guajava. The informant’s consensus of medicinal plant for diarrhea treatment was 0,38. The commonly used parts was the leaves and most of the used methods were administered orally. The conclusions of this study were ethnic groups in South Sulawesi has various formula of medicinal plants for diarrhea treatment, but P.guajava was the most commonly used. Those formulas information for diarrhea treatment would be an alternative to overcome diarrhea problems in South Sulawesi. \u0000  \u0000Key words: medicinal plant, traditional healer, diarrhea, \u0000  \u0000ABSTRAK \u0000Pengetahuan lokal dalam pemanfaatan tumbuhan obat untuk mencegah dan mengatasi penyakit telah dimiliki secara turun temurun oleh etnis-etnis di Sulawesi Selatan. Salah satunya adalah untuk mengatasi diare. Sulawesi Selatan termasuk dalam lima provinsi dengan insiden maupun period prevalence diare tertinggi di Indonesia. Tujuan dari penelitian ini adalah untuk mengungkap  jenis-jenis tumbuhan obat yang digunakan hatttra di Sulawesi Selatan dalam ramuan anti diare dan bukti ilmiah penggunaan tanaman obat tersebut untuk mengatasi diare. Studi ini menggunakan data sekunder yang diperoleh dari Eksplorasi pengetahuan lokal etnomedisin dan tumbuhan obat berbasis komunitas di Indonesia pada tahun 2012,2015 dan 2017. Analisis data dilakukan untuk mengetahui Fidelity level, Used value, Choice value, Factor of informant’s consensus dan studi referensi ilmiahnya. Hasil studi menunjukkan informasi tentang tiga puluh tanaman obat untuk mengatasi diare yang  diperoleh dari 48 penyehat tradisional yang berasal dari 19 etnis di Sulawesi Selatan. Informasi tersebut termasuk nama tanaman, bagian yang digunakan, dan metode persiapan. Fidelity level yang tertinggi adalah 41,67% untuk Psidium guajava, disusul 8,33% ","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84314335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFEK REBUSAN RIMPANG SEGAR, REBUSAN RIMPANG KERING, MINYAK ATSIRI DAN KURKUMIN Curcuma xanthorriza Roxb. TERHADAP KADAR BILIRUBIN PADA TIKUS WISTAR JANTAN YANG DIINDUKSI PARASETAMOL","authors":"F. Arifah, S. Pramono, A. Nugroho","doi":"10.22435/jtoi.v11i2.572","DOIUrl":"https://doi.org/10.22435/jtoi.v11i2.572","url":null,"abstract":"Javanese turmeric (Curcuma xanthorrhiza Roxb.) is a medicinal plant that can be developed for the treatment of liver disease. The aim of this study is to evaluate the decoction of fresh rhizome, decoction of dried rhizome, essential oils, and curcumin of Curcuma xanthorrhiza Roxb. against bilirubin level on paracetamol-induced male Wistar rats. Male Wistar rats were divided into 10 groups including group I and II were normal control and negative control, groups III, IV, and V received pretreatment of decoction of fresh rhizome 0,75 g/kgBW, 2,25 g/kgBW, and 6,75 g/kgBW, respectively for 9 days, Groups VI, VII, and VIII received pretreatment of decoction of dried rhizome 0,45 g/kgBW, 1,35 g/kgBW, and 4,05 g/kgBW, respectively for 9 days, group IX received pretreatment of essential oils 1,01 μl/kgBW for 9 days, group X received pretreatment of curcumin 75 μg/kgBW for 9 days. Groups II, III, IV, V, VI, VII, VIII, IX, and X on days 7, 8, and 9 induced paracetamol 3 g/kgBW. The blood was drained on day 0 and 4 after induction of paracetamol for bilirubin parameter study. Data were analyzed statistically using One-Way ANOVA test followed by LSD test with a 95% confidence level. The results showed that pretreatment of decoction of fresh rhizome 0,75 g/kgBW decrease the bilirubin level significantly (p<0,05) against the negative control. It can be concluded that pretreatment of decoction of fresh rhizome 0,75 g/kgBW was the best effect of decrease the bilirubin level.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77383493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}