Jurnal Kimia Valensi最新文献_第7页

Extraction and Characterization of Urease from Durio zibethinus L 牛粪中脲酶的提取及性质研究

Jurnal Kimia Valensi Pub Date : 2021-11-26 DOI: 10.15408/jkv.v7i2.21768

Zusfahair Zusfahair, A. Fatoni, D. Ningsih, M. Kurniasih

{"title":"Extraction and Characterization of Urease from Durio zibethinus L","authors":"Zusfahair Zusfahair, A. Fatoni, D. Ningsih, M. Kurniasih","doi":"10.15408/jkv.v7i2.21768","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.21768","url":null,"abstract":"Urease is a biocatalyst that serves to hydrolyze urea into ammonia and carbon dioxide. Since it is an imported product, the price of urea is still high. Urease can be found in grains. One of the grains that has not been explored for its urease content is durian (Durio zibethinus L.) seeds. This study aims to determine the effect of germination time on the activity of urease from durian seeds and its characteristics including the effect of pH, incubation temperature, enzymatic reaction time, addition of EDTA and metals, and storage time on the activity of urease from durian seeds. The first step of this study was seed germination which was carried out in the dark for 0, 3, 5, 7, and 9 days. Durian seed sprouts were extracted by mashing them using a mortar and pestle. They were then homogenized using a stirrer and centrifuged in a cold state. The crude urease extract obtained was then tested for its activity using the Nessler method. The acquired data was tested statistically using ANOVA. The results showed that the activity of urease from durian seeds was optimum at 3-day germination time, pH 7, incubation temperature at 30 °C, and 15-minute enzymatic reaction time with an activity of 163.6 U/mL. Urease is a metalloenzyme with its inhibitor being the Cu2+ and Na+ metal ions and its activator being Ba2+ metal ion. Tukey's test analysis showed that the effect of urease storage time at 4 °C resulted in a stable urease activity for 8 days while at room temperature it decreased its activity significantly to 72.8%.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47124032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Adsorbent from Bagasse for Methylene Blue Adsorption 甘蔗渣合成亚甲基蓝吸附剂的研究

Jurnal Kimia Valensi Pub Date : 2021-11-26 DOI: 10.15408/jkv.v7i2.20916

N. Nurhasni, Sariana Harahap, A. Fathoni, H. Hendrawati

{"title":"Synthesis of Adsorbent from Bagasse for Methylene Blue Adsorption","authors":"N. Nurhasni, Sariana Harahap, A. Fathoni, H. Hendrawati","doi":"10.15408/jkv.v7i2.20916","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.20916","url":null,"abstract":"The ability of bagasse adsorbents to adsorb methylene blue without activation using 0.5 M H2SO4 solution was examined. Methylene blue is widely used in the textile industry because it produces bright colors, and the dyeing process is fast and easy. This research aims to determine the optimum adsorption conditions, namely the variations in contact time, dye concentration, adsorbent mass, and pH effect on methylene blue, which were carried out using the batch method. Furthermore, the adsorbents were characterized by FT-IR and SEM. The optimum state of the bagasse adsorbent to adsorb methylene blue dye has a mass of 0.5 grams, a contact time of 30 minutes, a concentration of 50 ppm, and a pH of 5. The character of the adsorbent after activation with H2SO4 was better than without activation. The highest adsorption efficiency of methylene blue dye in the batch method was 99.67%. The FTIR spectrum of the bagasse adsorbent showed OH, C-H, C=O, C=C, and C-O functional groups. The adsorption isotherm model for methylene blue dye follows the Langmuir isotherm since the graph obtained is linear with the correlation coefficient (R2) = 1, where the adsorbent has a homogeneous surface.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44905998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Degradation of Imidacloprid Residue on Red Tomatoes (Solanum lycopersicum) by Advanced Oxidation Processes and Analysis using Spectrophotometer and HPLC 高级氧化法降解红番茄(Solanum lycopersicum)中吡虫啉残留及分光光度计和高效液相色谱分析

Jurnal Kimia Valensi Pub Date : 2021-11-25 DOI: 10.15408/jkv.v7i2.21630

Safni Safni, Hazanita Jumiaty, H. Aziz

{"title":"Degradation of Imidacloprid Residue on Red Tomatoes (Solanum lycopersicum) by Advanced Oxidation Processes and Analysis using Spectrophotometer and HPLC","authors":"Safni Safni, Hazanita Jumiaty, H. Aziz","doi":"10.15408/jkv.v7i2.21630","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.21630","url":null,"abstract":"The insecticide imidacloprid (C9H10ClN5O2) common used by farmers to control pests on red tomato plants, is a dangerous substance classified as a Class II toxic. The imidacloprid residue in red tomatoes enters the body, it will lead to health problems. The purpose of this study was to determine the percentage of imidacloprid residue that can be degraded using the Advanced Oxidation Processes (AOPs) method, which includes sonolysis, sonozolysis, ozonolysis, ozone water, and the effect of various parameters. Processing time, water volume, and red tomato mass were the test parameters studied. The change in imidacloprid residue concentration during the degradation process was measured using a UV/Vis spectrophotometer (double beam) with a wavelength of 200-400 nm and HPLC with mobile phase composition used was acetonitrile/water (65:35 v/v). With a processing time of 10 minutes, the imidacloprid residue in red tomatoes can be degraded 57.38% by sonozonolysis, 63.51 % by sonolysis, 85.17 % by ozonolysis, and 88.76 % by ozone water. The imidacloprid residue in 75 g of red tomatoes could be removed as much as 91.65% by treating with ozone water for 15 minutes. HPLC analysis showed that no intermediate compounds were detected in the imidacloprid residue degradation process in red tomatoes.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44923390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Fourier Transform Infrared Spectrophotometry Method for Analysis of Metformin Hydrochloride in Marketed Tablet Dosage Form 傅里叶变换红外分光光度法分析市售片剂中盐酸二甲双胍的含量

Jurnal Kimia Valensi Pub Date : 2021-11-25 DOI: 10.15408/jkv.v7i2.22158

N. Nerdy, Linda Margata, Dian Ika Perbina Meliala, Bunga Mari Sembiring, Selamat Ginting, E. D. Putra, Atika Mulyati, T. K. Bakri

{"title":"Application of Fourier Transform Infrared Spectrophotometry Method for Analysis of Metformin Hydrochloride in Marketed Tablet Dosage Form","authors":"N. Nerdy, Linda Margata, Dian Ika Perbina Meliala, Bunga Mari Sembiring, Selamat Ginting, E. D. Putra, Atika Mulyati, T. K. Bakri","doi":"10.15408/jkv.v7i2.22158","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.22158","url":null,"abstract":"The first line drug given for monotherapy for diabetes mellitus type 2 is metformin hydrochloride, which is a biguanide antihyperglycemic drug. The aim of this research was to develop, validate, and apply the Fourier Transform Infrared spectrophotometry method to identify and determine metformin hydrochloride in marketed tablet dosage form. This research included preparation of standard, analysis of samples, and validation of method. The specific wavenumber obtained for qualitative analysis was 1645.68 cm–1 and 1574.8 cm–1. The specific area obtained for quantitative analysis with a single baseline ranged from 1701.53 cm–1 to 1535.66 cm–1. All metformin hydrochloride marketed tablet dosage forms were analyzed and met all of the qualitative and quantitative requirements. The methods met the requirements of method validation for accuracy with a percentage of recovery of 100.22 %, precision with relative standard deviation of 0.48 %, linearity with a correlation coefficient of 0.9992, limit of detection of 11.17 mg per mL, limit of quantitation of 33.84 mg per mL, and good specificity results. In this study, the Fourier Transform Infrared spectrophotometry method was successfully developed and validated for application in identification and determination of metformin hydrochloride in marketed tablet dosage form.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45928233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Dynamics Simulation of Bioactive Compounds Against Six Protein Target of Sars-Cov-2 As Covid-19 Antivirus Candidates 抗新冠肺炎候选病毒Sars-Cov-2六蛋白靶点生物活性化合物的分子动力学模拟

Jurnal Kimia Valensi Pub Date : 2021-11-25 DOI: 10.15408/jkv.v7i2.21634

Fikry Awaluddin, I. Batubara, S. T. Wahyudi

{"title":"Molecular Dynamics Simulation of Bioactive Compounds Against Six Protein Target of Sars-Cov-2 As Covid-19 Antivirus Candidates","authors":"Fikry Awaluddin, I. Batubara, S. T. Wahyudi","doi":"10.15408/jkv.v7i2.21634","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.21634","url":null,"abstract":"Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the virus that causes Coronavirus 2019 (COVID-19). To date, there has been no proven effective drug for the treatment or prevention of COVID-19. A study on developing inhibitors for this virus was performed using molecular dynamics simulation. 3CL-Pro, PL-Pro, Helicase, N, E, and M protein were used as protein targets. This study aimed to determine the stability of the selected protein-ligand complex through molecular dynamics simulation by Amber20 to propose bioactive compounds from natural products that have potential as a drug for COVID-19. Based on our previous study, the best value of free binding energy and protein-ligand interactions of the candidate compounds are obtained for each target protein through molecular docking. Corilagin (-14.42 kcal/mol), Scutellarein 7-rutinoside (-13.2 kcal/mol), Genistein 7-O-glucuronide (-10.52 kcal/mol), Biflavonoid-flavone base + 3O (-11.88 and -9.61 kcal/mol), and Enoxolone (-6.96 kcal/mol) has the best free energy value at each protein target. In molecular dynamics simulation, the 3CL-Pro-Corilagin complex was the most stable compared to other complexes, so that it was the most recommended compound. Further research is needed to test the selected ligand activity, which has the lowest free energy value of the six target proteins.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43729306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Ultrasonic Assisted Extraction Process on Antioxidant Activity of Honje Fruit Extract (Etlingera elatior) Using Surface Response Method 表面响应法优化超声辅助提取工艺对胡麻提取物抗氧化活性的影响

Jurnal Kimia Valensi Pub Date : 2021-11-24 DOI: 10.15408/jkv.v7i2.21396

Artta Gracia Malau, A. Widyasanti, S. Putri

引用次数: 1

In Silico Analysis of Glucose Oxidase H516r and H516d Mutations for an Enzymatic Fuel Cell 酶促燃料电池葡萄糖氧化酶H516r和H516d突变的原位硅分析

Jurnal Kimia Valensi Pub Date : 2021-11-23 DOI: 10.15408/jkv.v7i2.20733

P. Puspita, L. Ambarsari, Adrian Adiva, T. Sumaryada

引用次数: 2

Photocatalytic Degradation of Methylene Blue and Methyl Orange by Y-PTC Metal-Organic Framework Y-PTC金属有机骨架光催化降解亚甲基蓝和甲基橙

Jurnal Kimia Valensi Pub Date : 2021-11-23 DOI: 10.15408/jkv.v7i2.22267

A. Adawiah, Muhammad Derry Luthfi Yudhi, A. Zulys

{"title":"Photocatalytic Degradation of Methylene Blue and Methyl Orange by Y-PTC Metal-Organic Framework","authors":"A. Adawiah, Muhammad Derry Luthfi Yudhi, A. Zulys","doi":"10.15408/jkv.v7i2.22267","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.22267","url":null,"abstract":"The yttrium based metal-organic framework (MOF) Y-PTC was synthesized by the solvothermal method using perylene as the linker and yttrium as metal ion. This study aims to assess the photocatalytic activity of yttrium-perylenetetracarboxylate (Y-PTC) metal-organic framework (MOF) toward methylene blue and methyl orange under visible light irradiation. The results of the FTIR analysis showed that Y-PTC MOF had a different structure and composition from its precursor (Na4PTC). The Y-PTC MOF has a bandgap energy value of 2.20 eV with a surface area of 47.7487 m2/g. The SEM-EDS analysis showed an elemental composition of yttrium, carbon, and oxygen, were 6.9%, 72.1% and 20.7%, respectively. Furthermore, Y-PTC MOF was able to adsorb dyes at the optimum by 78.10% and 35.57% toward methylene blue (MB) and methyl orange (MO) at the dispersion period of 60 mins. Y-PTC MOF exhibited photocatalytic activity towards the degradation of methylene blue and methyl orange under visible light irradiation. The addition of H2O2 inhibited Y-PTC photocatalytic activity towards MO degradation from 50.89% to 26.38%. In contrast to MO, the addition of H2O2 had a positive effect on MB, which increased the degradation from 87.56% to 91.65%. Therefore, Y-PTC MOF possessed the potential of a photocatalyst material in dyes degradation under visible light irradiation.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43453211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Photocataytic Degradation of Phenol Using TiO2-Fe Under H2O2 Presence by Visible and Sunlight Irradiation 可见光和阳光照射下H2O2存在下TiO2-Fe光催化降解苯酚

Jurnal Kimia Valensi Pub Date : 2021-11-23 DOI: 10.15408/jkv.v7i2.20766

L. J. Kusumawardani, A. Iryani

{"title":"Photocataytic Degradation of Phenol Using TiO2-Fe Under H2O2 Presence by Visible and Sunlight Irradiation","authors":"L. J. Kusumawardani, A. Iryani","doi":"10.15408/jkv.v7i2.20766","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.20766","url":null,"abstract":"Phenol is one of the essential organic pollutants released into the environment because of its high stability and toxicity. It is harmful to organisms, environment, and posing a serious threat to human health at low concentration. This research investigated the photocatalytic degradation process of phenol using a TiO2-Fe catalyst under visible light irradiation and additional H2O2. The effect of various conditions process was applied, including different catalyst doses (0.2, 0.4, 0.6, and 0.8 g/L), pH (3, 6, 8, and 11), irradiation times (60, 90, 120, 150, and 210 minutes) and the presence of H2O2. The degradation process was studied at an initial concentration of phenol 5 mg/L. This study has been decreasing phenol content (90.51%) with catalyst doses 0.6 g/ L sample solution, pH solution 11, reaction time 210 minutes and H2O2 concentration 30%. This final phenol concentration after photodegradation under halogen light was 0.18 mg/L, while sunlight irradiation was 0.11 mg/L. This result is below government regulation as per Permen LH RI No. 5/2014 i.e. 0.5 mg/L. Therefore, this process possible to remove phenol in aqueous such as industrial wastewater or other resources.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47594257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and Characterization of Nano-sized Carbonated Calcium Hydroxyapatite (CHAp) from Rebon shrimp (Acetes erythraeus) as a Candidate for Dental Restoring Application 红虾纳米碳化羟基磷灰石(CHAp)的合成与表征

Jurnal Kimia Valensi Pub Date : 2021-11-23 DOI: 10.15408/jkv.v7i2.21359

N. Ngatijo, R. Bemis, Heriyanti Heriyanti, R. Rahmi, Nashihul Ulwan, R. Basuki

{"title":"Synthesis and Characterization of Nano-sized Carbonated Calcium Hydroxyapatite (CHAp) from Rebon shrimp (Acetes erythraeus) as a Candidate for Dental Restoring Application","authors":"N. Ngatijo, R. Bemis, Heriyanti Heriyanti, R. Rahmi, Nashihul Ulwan, R. Basuki","doi":"10.15408/jkv.v7i2.21359","DOIUrl":"https://doi.org/10.15408/jkv.v7i2.21359","url":null,"abstract":"Carbonated calcium hydroxyapatite (CHAp) exhibits excellent biocompatibility with bone and teeth, making it an ideal candidate for orthopedic and dental application. However, the study of CHAp synthesis from natural material is still scarce. The purpose of this research is to synthesize and characterize of CHAp, using Rebon shrimp (Acetes erythraeus) as a calcium source. The synthesis was conducted by hydrothermal method with the variation of Ca/P ratios 1.61; 1.67; 1.73. The as-prepared CHAp was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy-energy dispersive X-ray (SEM-EDX). The FT-IR results show that synthesized material exhibited characteristic CHAp band of hydroxide at 3448 and 1635 cm-1, carbonate at 872 and 1427 cm-1, and phosphate at 1049; 606; and 570 cm-1. The diffractogram pattern assigned the all observed peak of CHAp are in good agreement compared to CHAp database with the nano-scale size. It also observed that the high Ca/P ratio will decrease the crystallinity of CHAp. The as-prepared CHAp micrograph is agglomerates spherical form with size between 5-20 nm which build up from 18–26 nm crystallite particles. The result of this research confirmed that Rebon shrimp is the promising materials for calcium source in CHAp production.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46971665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0