Jurnal Ilmiah Perikanan dan Kelautan最新文献

{"title":"Optimization of Medium Composition for Streptomyces sp. PB2 Chitinase Production using Response Surface Methodology","authors":"Anandita Perwita Kurniawan, I. D. Puspita, A. Husni","doi":"10.20473/jipk.v14i1.27602","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.27602","url":null,"abstract":"Highlight ResearchThe chitinase production by Streptomyces sp. PB2 was studiedThe critical medium component for chitinase production were identifiedThe optimum medium composition for chitinase production was obtainedMedium optimization improved chitinase production by 6-fold increase in activityAbstractChitin is a polysaccharide compound composed of N-acetylglucosamine (NAG), which is linked by β-1,4-glycoside bonds. In producing NAG from chitin, enzymatic method using chitinase offer advantages compared to chemical degradation. Streptomyces sp. PB2 is a good candidate of chitinase producer which was previously isolated from shrimp pond sediment. However, optimization of chitinase production by Streptomyces sp. PB2 is required for large-scale production of this enzyme. This study aimed to find the optimal medium composition to increase the chitinase enzyme activity of Streptomyces sp. PB2 using the Response Surface Method. Initial screening was done to determine additional carbon and nitrogen sources in colloidal chitin broth suitable for increasing chitinase activity. Optimization of the medium composition was conducted using the Plackett-Burman design to determine the critical components in the colloidal chitin broth medium and continued by Box-Behnken model to optimize the concentration of the medium components. Chitinase activity was obtained by measuring the amount of reducing sugar (NAG) released from enzymatic reaction using DMAB reagent by means of spectrophotometer. The medium components showing high contribution in increasing chitinase activity were K2HPO4, colloidal chitin and peptone, with the confidence level value of 0.66, 0.48, and 0.38, respectively. The Box-Behnken model analysis shows that the combination of K2HPO4 0.007 g/ml, colloidal chitin 1.5 g/ml and peptone 1.5 g/ml in colloidal chitin broth are the optimal medium for Streptomyces sp. PB2, resulted in chitinase activity of 0.0125 U/ml. The increase of 6-fold in chitinase activity was achieved in this study.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43245453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UFLPlus: An Underwater Fish Lamp Technology as an Innovation of Fish-Luring Aids on Boat Lift Net","authors":"F. Fajriah, Ahmad Mustafa, M. Rais, La Anadi, K. T. Isamu, Marsuki Iswandi, I. Salam, Sampunur Sampunur","doi":"10.20473/jipk.v14i1.30074","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.30074","url":null,"abstract":"Highlight ResearchDipped lamp technology with CCTV (UFLPlus) to catch fish was designed in such a way that the placement of components contained was integrated, water-proof, and having a good light distribution.The existence of a CCTV in new innovated UFLPlus that can record events in the water and greatly assist fishing activities of fishermen on passive fishing gear such as boat charts.UFLPlus worked well to increase the number of hauling every night of fishing (from 20-30 kg to 50-60 kg per hauling).AbstractThe usage of underwater immersion lights outfitted with CCTV is a technological innovation that is expected to solve the inadequacies of the acoustic work system while also improving the existing underwater immersion light work system. Underwater Fish Lamp Plus (UFLPlus) technology can attract attention and directly monitor the condition of fish in the sea from the boat. The purpose of this study was to understand how the UFLPlus was designed and constructed; to test its performance using lamplight, waterproof, and immersion tests; to learn how the light distribution pattern is formed; and to learn the state of the catch. The study was separated into two phases: the design and fabrication of UFLPlus, and numerous laboratory-scale experiments and outdoor trials with boat lift net fishing gear. The results reveal that UFLPlus performed effectively in the field after all of its physical components were tested in the laboratory, and that the values of light intensity and light distribution patterns are still in accordance with the level of adaptation to light reception on the fish retina. During the trial catch, 62 kg of anchovy (Stolephorus sp.), 27 kg of ponyfishes (Leiognathus sp.), 32.5 kg of fringe-scale sardinella (Sardinella fimbriata), and 17.5 kg of yellow-stripe scad (Selaroides leptolepis) were caught. UFLPlus was capable of functioning as an underwater immersion lamp that attracts fish attention and directly monitors the arrival of fish in the water in the fishing area.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45330578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric and Meristic Characterization of Hairtails Trichiurus lepturus Linnaeus, 1758 (Scombriformes: Trichiuridae) from the Northern Coast of Java, Indonesia","authors":"Adhimas Febri Aryantojati, Murwatoko Murwantoko, E. Setyobudi","doi":"10.20473/jipk.v14i1.31443","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.31443","url":null,"abstract":"Highlight ResearchThe most significant morphological variation was the snout length and caudal peduncleT. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same populationT. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species AbstractHairtails (Trichiurus spp.) are economically important fisheries resources and have become an export commodity. In Indonesia, there are several hairtail species; however, each species distribution is not yet certainly known. This study aimed to identify and determine the morphometric and meristic character of the hairtail from the northern coast of Java, Indonesia. Fish specimens were obtained from fishermen catches in the north coast of Pati and Demak (Central Java) and the northern coast Cirebon (West Java), which captures the fish in less than one-week fishing operation. Fish samples were grouped based on morphological similarities identified in previously published works. After that, 22 morphometric and four meristic characters were measured and counted. Principal Component Analysis and Discriminant Analysis were used to analyze morphometric data, while the meristic data were compared to the available published works. The hairtail caught at the northern coast of Java was long, flat, silvery in color, and had a sagittal crest, with the fin formula being D.III.125-134, P.I.10, and A.I.98-104. According to the morphological and meristic characters, the hairtail caught at Java’s northern coast was identified as Trichiurus lepturus. The most significant morphological variation was the snout length and caudal peduncle. The PCA and scattergram analyses showed that T. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same population.  T. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47955492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redescription of Bactronophorus thoracites Gould (1856) and Bankia gracilis Moll (1935) from Sabah Waters, Malaysia, with Short Ecological Notes and Measurements Metrics","authors":"Loo Zhen Ann, Chen Cheng Ann, K. A. Rahim, F. Diba","doi":"10.20473/JIPK.V14I1.26825","DOIUrl":"https://doi.org/10.20473/JIPK.V14I1.26825","url":null,"abstract":"Highlight ResearchRedescription of marine woodborers, Bactronophorus thoracites and Bankia gracilis from the region were illustrated.New records of Bactronophorus thoracites and Bankia gracilis from the region.Addition info on the measurements of pallets was recorded.Certain ecological data on the habitat of marine woodborers Bactronophorus thoracites and Bankia gracilis were documented.AbstractMollusc wood-borers are classified into two groups: pholads and teredinids. While pholads have a limited distribution to temperate and tropical marine waters, teredinids are found worldwide. However, limited info on the taxonomy was documented on the marine wood borer in Sabah, Malaysia. Present study focuses on determining the wood borer species from Sabah waters, Malaysia. Samples were collected from fallen tree debris in the mangrove at Kota Kinabalu, Kuala Penyu and W.P. Labuan during low tide and water quality parameters were taken in-situ from all three sampling sites. Specimen identification was carried out by observing the physical characteristics of the pallets which are unique between genuses. The species Bactronophorus thoracites was identified from the Kota Kinabalu sampling site and species Bankia gracilis were found in both the Kuala Penyu and W.P. Labuan sampling site. The pallet of B. thoracite are of “dagger-and-sheath” shape, it is 26mm in length, with blade measuring 10mm in length, basal cup 4mm in length and stalk 12mm in length. The pallet of B.gracilis is characterized by the dark periostacum covering the 3-lobbed upper margin of the calcareous portion of the inner face of the cone, the pallet is 20mm in length, with the blade and stalk both measuring 10mm in length. The present study described two species of marine wood borers namely B. thoracites and B. gracilis in Sabah and W.P. Labuan with some measurement metrics and ecological parameters that were missing from previous studies that aided the process of species identification in future.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48895437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon Sink Estimation of Mangrove Vegetation Using Remote Sensing in Segara Anakan, Cilacap","authors":"Z. Aulia, R. Hidayat, A. Amron","doi":"10.20473/jipk.v14i1.31789","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.31789","url":null,"abstract":"Highlight ResearchThe value of Leaf Area Index (LAI) and Normalized Different Vegetation Index (NDVI) have a strong relationship.Estimated mangrove leaf biomass and carbon stock in mangrove can be known through the Leaf Area Index (LAI) value.Each mangrove pixel can absorb the different carbon.Increasing the diameter of the tree trunk shows the more CO2 absorbed by the tree.AbstractWorldwide global warming occurs because of increasing carbon dioxide (CO2) in the atmosphere. Segara Anakan, the largest mangrove forest in Java Island, has the highest potential as a carbon sink in the tropics. This study aimed to quantify the area and density of mangrove forests in Segara Anakan and to estimate their potentials as carbon sink based on Sentinel-2A imagery. In this study, data from Sentinel 2A satellite imagery was processed and validated with field survey results. The results showed that Segara Anakan had mangrove forests covering an area of 5,481.64 ha in 2019 with the sparse density covered 706.92 ha, medium density covered of 2,526.80 ha, and high density covered 2,848.60 ha. The results of mangrove density were validated using Table Confusion Matrix and using correlation coefficient value (r) by comparing field measurement and Normalized Difference Vegetation Index (NDVI) algorithm. The accuracy for mangrove density was 86.92% with r-value 0.85. Mangrove in Segara Anakan has the ability to absorb 8.56 Mega gram of carbon per hectare. Segara Anakan mangroves have the potential to absorb high level of carbon, so we should maintain their sustainability.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49357586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-chitosan Spray as a Preservative and Food Security of Fishery Products in The Middle of the Covid-19 Pandemic","authors":"Dion Saputra, Ferra Robiatul Ula, Ajeng Budiarahma Nur Fadhila, Yosi Yananda Sijabat, Abista Ahmad Romadoni, S. Windarto","doi":"10.20473/jipk.v14i1.30121","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.30121","url":null,"abstract":"Highlight ResearchThe author mentioned 2 highlights from their results research  Nano-chitosan has the best susceptibility to bacterial strains of E. coli, S. enteritidis, L. monocytogenes, B. cereus and S. aureus.Nano-chitosan showed higher degree of inhibition than that done by chitosan. AbstractThe COVID-19 pandemic has impacted the fisheries sector, a decline in exports and fishermen's income caused by the disconnection of the marketing chain due to lockdown implementation in several export destination countries. Fish is a source of protein and as perishable goods, it experiences quality damage due to spoilage, commonly caused by hampered distribution of catches. Natural preservatives are needed to preserve catch so it won’t get spoiled and can be accepted by consumers in suitable conditions for consumption. This study aims to evaluate the particle size of nano-chitosan and determine the effectiveness of nano-chitosan spray with different concentrations as a natural preservative in caught fish. This research method begins with making chitosan through 3 stages: demineralization, deproteination, and deacetylation. Chitosan was made into nano-chitosan with various concentrations of 3:1, 4:1, and 5:1 using ionic gelation. Nano-chitosan underwent several tests, including PSA, antibacterial activity by disc diffusion, organoleptic, and the effectiveness of preservatives. Antibacterial activity of nano-chitosan was able to inhibit Bacillus subtilis at three different concentrations, the potential to inhibit Escherichia coli was optimal at 5:1 treatment. The effectiveness of nano-chitosan preservative bacteria inhibition at three different concentrations proved to be sufficient to be used to extend shelf life and ensure the safety and quality of fishery products. The best concentration of nano-chitosan was 3:1 treatment. Nano-chitosan spray from crab shell waste has good antibacterial activity and preservative effectiveness. It could serve as an antibacterial agent and natural preservative for fishery products during the COVID-19 pandemic.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44672172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Price Behavior of Tuna in Indonesia","authors":"K. Sukiyono, P. S. Asriani, Redy Badrudin, Nola Windirah, R. Yuristia, Musriyadi Nabiu","doi":"10.20473/jipk.v14i1.30100","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.30100","url":null,"abstract":"Highlight ResearchPrices are often a signal for producers to produce or market products, including the price of tuna.Four price behaviors, namely trends, seasonal variation, variability and instability, and market integration, are important to study in designing business development plans.Tuna prices in Indonesia tend to have a statistically positive trend.Tuna prices tend to be stable, or do not have high variability, throughout the observations and indicate a low price risk.The price of tuna fish in each port is formed independently and is not influenced and influences each other between the markets observed.AbstractPrices of agricultural and fisheries products have always been a concern not only by producers and consumers but also by the government in designing policies to control and stabilize these prices to protect the poor. Therefore, a study of price behavior is crucial to be scrutinized more deeply and comprehensively. This research is aimed at examining and analyzing tuna (Thunnus spp.) price behavior in terms of their seasonal, trend, variability, and instability behavior as well as their market integration. For this purpose, monthly data of tuna prices from 2011:1 to 2019:12 in five tuna production centers (provinces) in Indonesia, including North Sulawesi, West Papua, Aceh, North Maluku, and Bali, at the national level were applied.  It was found that tuna prices have a positive trend and are statistically significant, excluding in West Papua. The study also found that price variability and instability were insignificant during observations. The study also concluded that the tuna price in every single province in Indonesia is formed independently.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49563718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Homogenization Process on the Production of Arthrospira platensis Carotenoid Nanocapsules Encapsulated with Arabic Gum and Whey Protein Concentrate","authors":"Khusnul Alfionita, S. A. Budhiyanti, N. Ekantari","doi":"10.20473/jipk.v14i1.32029","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.32029","url":null,"abstract":"Highlight ResearchThe homogenization process affected the particle size reductionThe homogenization process using both High Speed Homogenizer and Ultrasound had a smaller particle size and the highest efficiency in encapsulation AbstractArthrospira platensis contains a high concentration of carotenoids mainly 80 % β-carotene. The use of β-carotene in functional food and nutritional supplements was constrained by its lack of chemical and physical stability. Therefore, efforts were needed to protect carotenoids from damage by using an encapsulation system. The emulsion preparation before the encapsulation process had an influence on the stability of the oil-in-water emulsion. Various methods were used to prepare emulsions, such as high shear homogenizers (HSH) and ultrasound (US) treatment. The homogenization step was critical because it influenced the chemical stability of any encapsulated compounds. The purpose of this work was to generate nanocapsules of β-carotene with minimum carotenoid degradation, high efficiency of encapsulation, and small particle size, with variation in the homogenization process. This study consisted of 5 treatments, including the homogenization processes used HSH 24.000 rpm for 90 s ; HSH 24.000 rpm for 60 s, amplitude 55 % for 120 s ; amplitude 94 % for 138 s ;  amplitude 55 % for 138 s ;  and  amplitude 55 % for 120 s . Each parameter was analysed by ANOVA followed by Tukey pairwise comparisons with 95% confidence level and p<0.05. The particle size was influenced by the emulsification process, such as homogenization treatment, homogenization time, amplitude, and time of sonication. The sample that was homogenized using both HSH and US had a smaller particle size and the highest efficiency in encapsulation than others. The combination of homogenization process could decrease particle size.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42560667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine Biodiversity of Coral Reef Fishes in Pieh Marine Recreational Park After Bleaching and Acanthaster Outbreaks","authors":"R. Putra, M. Abrar, R. M. Siringoringo, N. W. Purnamsari, Pradipta Agustina, M. J. Islam","doi":"10.20473/jipk.v14i1.30133","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.30133","url":null,"abstract":"Highlight ResearchAfter bleaching disturbance and Acanthaster planci outbreaks, herbivore fishes species were dominated in MRP PiehThis explains the marine recreational activities in utilization zone MRP Pieh have no impact on reducing fish biomassAfter disturbance in MRP Pieh shows the coral reef fishes still endurance on bleaching event, and Acanthaster planci outbreak after disturbance in MRP Pieh shows the coral reef fishes still endurance on bleaching event and Acanthaster planci outbreak. However, the composition of corallivorous has decreased but has been an increase in herbivorous and carnivorous fish populationsAbstractPieh MRP encountered non-anthropogenic phenomena, precisely massive coral bleaching in 2016, 2017, and massive predators from Acanthaster planci outbreaks in 2018. This study aimed to understand the pattern of coral reef diversity in the core zone and utilization in the MRP area and compare it to non-MRPS locations that accept the same non-anthropogenic pressure conditions. Coral fish sampling using a UVC is categorized into three zones: the core zone, the utilization zone, and outside the MRP area. 8 Families of coral reef fishes were counted based on categories of level function in ecologies and economy. Taxonomic distinctiveness estimates were calculated mathematically for each sample, including species richness and taxonomic diversity were compared among zonation area. Pearson’s Coefficient Correlation Matrix was used to measure the correlation relationship between zonation areas. There are 91 species of fish and 3002 individuals found. The richest family in the MRP Core Zone and MRP Utility Zone was Acanthuridae with 20 species and non-MRP has a lower species richness and abundance of fish communities. The dominant species in Pieh MRP was Ctenochaetus striatus with average abundant per site (21.3 ± 7.62, n = 3). Acanthuridae represents 55.98% of the total biomass in MRP-Core Zone, 63.13% in MRP-Utility Zone, and 41.55% in Non-MRP Area. This study showed the number of species and populations from corallivores fishes have decreased but has been an increase in herbivorous and carnivore diversity. The diversity indices (H’) and ENS also shows no differ significantly between zonation.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45268774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Response of White Shrimp (Litopenaeus vannamei) to Different Density and IMNV Challenge","authors":"Nur Komariah Baladrat, M. Nurhudah, Heny Budi Utari","doi":"10.20473/jipk.v14i1.31468","DOIUrl":"https://doi.org/10.20473/jipk.v14i1.31468","url":null,"abstract":"Highlight ResearchWhite shrimp (Litopenaeus vannamei) challenged with IMNV and different stocking densitiesThe water quality parameter values are getting worse along with the increase in stocking densityWhite shrimp THC value decreased with increasing stocking density and IMNV challenge testThe development of the IMNV increased with an increase in stocking density seen from the speed and percentage of the appearance of the clinical symptoms and histopathology results AbstractIncreasing in stocking density of shrimp affects the physiology and behaviour of their moving space. The health condition of shrimp is influenced by feeding, growth, and its susceptibility on disease. The aim of this study was to determine the development of immune response in relation to density and the presence of IMNV infection. This study used a completely randomized design (CRD) at density of 100 shrimp.m-2, 200 shrimp.m-2, and 400 shrimp.m-2, with three replications in each treatment. The shrimp used was 5,02±0,26 g and the virus infection was exposed orally. This research was facilitated at the Disease Research Centre Laboratory of Central Proteina Prima Company, Pasar Kemis, Tangerang for 30 days. The results showed that the Total Hemocyte Count (THC) in hemolymph of shrimp had different values between negative controls and challenged IMNV. The lowest THC value was found at a density of 400 shrimp m-2 (3.00x106ml-1). While the highest THC value was at a density of 100 shrimp.m-2 (4.75x106ml-1). This result is supported by the increasing value of water quality parameters along with the increasing density of shrimp. Histopathology changes on skeletal muscle and lymphoid organs confirmed that the development of IMNV infection was faster at high shrimp densities.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46955883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}