Jurnal Ilmiah Perikanan dan Kelautan最新文献

{"title":"The Effect of Immersion Duration of Zoothamnium penaei Crude Protein Extracts to Stimulate Immune System in Litopenaeus vannamei Against Enterocytozoon hepatopenaei (EHP)","authors":"A. Nkuba, G. Mahasri, N. D. Lastuti, A. A. Mwendolwa","doi":"10.20473/jipk.v15i2.38349","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.38349","url":null,"abstract":"Highlight Research\u0000\u0000The effect of immersion duration of shrimp to the immunostimulant from crude protein Zoothamnium penaei was determined.\u000020-minute immersion duration in crude protein from Zoothamnium penaei was found to increase the phagocytosis activities, phenoloxidase, and improve hepatopancreases of the shrimp.\u0000There is increase in phagocytosis activities, phenoloxidase and improved hepatopancreases cell within a three-week maintenance of shrimp with immersion after each seven days.\u0000There is interaction between immersion duration and maintenance time of shrimp into the crude protein from Zoothamnium penaei.\u0000\u0000Abstract\u0000Enterocytozoon hepatopenaei (EHP) is a spore-forming microsporidia intracellular parasite, which slough off the cells of the hepatopancreases of the shrimp. This study was intended to determine the effect of immersion duration and maintenance time to stimulate the immune system of shrimps by using a crude protein from Zoothamnium penaei to protect against EHP infections. The study used a completely randomized design where shrimps were divided into four groups each having five replicates. Groups P1, P2, P3 were immersed in immunostimulant for 10, 15, and 20 minutes respectively, except negative control. The phenoloxidase, phagocytosis activities, histopathology study were determined. Also, the molecular diagnosis of EHP was measured to confirm EHP infection in the shrimp samplesThe phenoloxidase activity significantly increased after immersion for 20 minutes (P3) from 0.64-, 0.72-, and 0.78-units min-1 mil -1 of protein on the first week, second week and third week, respectively. The phagocytosis activities of the sample immersed for 20 minutes were significantly increased to 68% on the second week. The sample also showed improved hepatopancreases epithelial cells with only 26% necrotic cell, less EHP spore and vacuolation. There was an interaction between immersion duration and maintenance time of the L. vannamei immersed in crude protein from Zoothamnium penaei. Twenty minutes immersion were significantly found to stimulate the immune system of the shrimp against EHP. The present work revealed that, the application of crude protein from Z. penaei is effective against EHP in shrimp culture.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42172080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate Profile, Antioxidant, and Antibacterial Activities of Brown Algae Sargassum cristaefolium from Pane Island, North Sumatera","authors":"Astrid Fauzia Dewinta, I. E. Susetya, K. Khairunnisa, M. Suriani, Syahira Addina, A. Fadhilah","doi":"10.20473/jipk.v15i2.41621","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.41621","url":null,"abstract":"Highlight Research\u0000\u0000Alginate content produced by Sargassum cristaefolium in three different parts of the thallus has food grade quality.\u0000The alginate chemical profile of cristaefolium in three different thallus part had a significant effect.\u0000Antioxidant activity of cristaefolium extract was in the moderate category.\u0000Antibacterial activity of cristaefolium extract against aquatic pathogenic bacteria has moderate and strong effectiveness.\u0000\u0000Abstract\u0000Sargassum, an ubiquitous variant of algae in North Sumatera, has grown wildly on Pane Island with the type, namely, Sargassum cristaefolium. This study aimed to determine the characteristics of alginates, antioxidative ability, and antibacterial ability. Alginate was extracted via the acid pathway method from three different parts of the thallus and was then characterized physically and chemically. Antioxidant activity using the DPPH method and the total phenol with a gallic acid standard. Antibacterial activity using the well diffusion method. The characteristics of alginate include yields of 53.61% ± 2.21%, viscosity of 11.44 ± 1.54 cPs, whiteness index of 56.55 ± 1.71, acidity degrees of 9.83 ± 0.34, moisture content of 9.63% ± 0.53%, ash content of 23.17% ± 0.76%, Pb and Hg levels of 0.065 ± 0.01 ppm, 0.034 ± 0.003 ppm. The IC50 value of antioxidant activity being 134.408 ± 0.04 ppm, moderate category. Extract concentration of 8 mL can inhibit the E. coli and E. tarda with inhibitions of 10.60 ± 0.72, 14.03 ± 2.97 mm. Although other pathogenic bacteria can be inhibited optimally at an extract concentration of 10 mL with inhibitions of 11.03 ± 1.43, 9.07 ± 0.20, 11.20 ± 1.56, 12.80 ± 3.01 mm. Alginates isolated in S.cristaefolium from Pane Island met food grade standards except for its viscosity. S. cristaefolium from Pane Island is a moderate antioxidant. Likewise, its antibacterial ability was moderate however it was strong against aquatic gram-negative bacteria.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42105536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of Astaxanthin Content in Mixed Culture of Dunaliella sp. and Azospirillum sp. under Light Intensity Treatment","authors":"Ria Amelia, Wulan Rahmani Akmal, E. Suyono","doi":"10.20473/jipk.v15i2.38596","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.38596","url":null,"abstract":"Highlight Research\u0000\u0000The growth rate was higher when Chlorella was induced by Azospirillum brasilense.\u0000The carbohydrate content increased when combined with Azospirillum brasilense.\u0000Determination of astaxanthin accumulation in Haematococcus pluvialis.\u0000Define condition favoring astaxanthin accumulation in Haematococcus pluvialis.\u0000\u0000Abstract\u0000Dunaliella sp. is a potential natural source of carotenoid pigments such as astaxanthin, β-carotene, and lutein. Dunaliella sp. can also accumulate other valuable products such as glycerol and protein. Another species is Azospirillum sp., which is known as microalgal growth-promoting bacteria. These bacteria are often cultured with microalgae because they contain indole-3-acetic acid, which can significantly increase the growth of microalgae. This study aimed to examine the pigment content in mixed culture of Dunaliella sp. and Azospirillum sp. after being treated with different light intensity treatment. In this study, Dunaliella sp. were cultivated by mixing with Azospirillum sp. under light stress. Two treatments were performed at light stress intensity of 3000 and 6000 lx. Light intensity is widely used as an important parameter in cultivation, which can affect the growth and production of microalgal biomass. In addition, spectrophotometric UV-Vis based measurement was conducted to investigate every single pigment content in all treatments under light stress for eight days. The number of cells, carotenoid pigments, and astaxanthin had increased significantly. Pigments of chlorophyll a and chlorophyll b also significantly increased at lower light treatments. Based on the results, the bacterium Azospirillum sp. and high light intensity significantly increased the growth and cell division of microalgae. Therefore, the combination of Azospirillum sp. and light stress intensity in microalgae cultivation could increase the growth and pigment of Dunaliella sp.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46138700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated of Sex Reversal use 17α-methyltestosterone Induced Female, Orange-Spotted Grouper Epinephelus coioides","authors":"O. Carman, A. Iskandar, Ching-Fong Chang, Guan-Chung Wu, M. Muslim, D. Ramadhani","doi":"10.20473/jipk.v15i2.40624","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.40624","url":null,"abstract":"Highlight Research\u0000\u0000Sex reversal for orange-spotted grouper Epinephelus coioides\u0000The application of 17α-methyltestosterone induce sex change\u0000The stability of sex change need more investigation\u0000\u0000Abstract\u0000The occurance of hermaphrodites in grouper fish causes a scarcity of male parents, so an alternative is needed to accelerate sexchange to male at a young age. The present study was expected to scrutinize the mechanisms of sex-change in fish in the early change process, and whether the testis converted from immature ovary using 17α-methyltestosterone (MT) would recover after the termination of MT treatment. MT-induced sex-change and 5-aza-2’-deoxycytidine (5-Aza) were connected as DNA methylation inhibitors to comprehend the alternation of gonadal soma cells. The orange-spotted groupers were used  at the developmental ages and fed a diet containing MT at 50 mg/kg for three months and then a normal diet for a month. In the first week and second week fish injected with 5-Aza intraperitoneally during the MT-oral administration. Most of the fishes in the control group had immature ovaries, but all the females fed with MT, had immature spermatogenesis. However, one month after the withdrawal of MT treatment, the sex of the fish returned to female-like even though the fish have undergone MT-induced masculinization. This outcome demonstrates precocious sex-change from under yearling, orange-spotted grouper utilizing oral MT treatment is impermanent. All the females of 5-aza treatments showed no spermatogenic cells.  In this study, lower growth rates were demonstrated by the MT-treated groups. The impact of this metabolic change was clear after the end of the hormone oral administration since the decreased growth of the groups treated for three months.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48615868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity and Potential Bioactive Peptides from Skin Protein Hydrolysate of Yellowfin Tuna (Thunnus albacares)","authors":"Sitti Hardiyanti Rachman, Joko Santoso, S. H. Suseno","doi":"10.20473/jipk.v15i2.41625","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.41625","url":null,"abstract":"Highlight Research\u0000\u0000Immersion of tuna skin using NaHCO3 at low temperatures effectively reduces the fat content.\u0000The DH value, MW, and amino acid composition are in line with the antioxidant activity of tuna skin hydrolysate.\u0000The ABTS method showed the highest antioxidant activity in tuna skin hydrolysate.\u0000FPH of tuna skin shows functional group characteristics and amino acid composition that are similar to collagen hydrolysate products.\u0000FPH of tuna skin has the potential as a source of antioxidants, ACE inhibitors, and antifibrinolytics.\u0000\u0000Abstract\u0000The tuna fillet industry produces abundant skin by-products with high protein, which has the potential as a raw material for fish protein hydrolysate (FPH) for a source of bioactive peptides. Exploration of bioactive peptides from fish skin is generally from hydrolyzed gelatin and collagen. The study aimed to produce FPH directly from tuna skin as an antioxidant and identify potential bioactive peptides. The research began by defatting using multiple concentrations of NaHCO3 and immersion times. The defatted fish skin was produced as FPH by enzymatic hydrolysis method using different papain enzyme concentrations and hydrolysis times. The selected treatments were assessed for antioxidant activity and bioactive peptides. The results showed that the defatting process using a 0.50% NaHCO3 for 30 minutes generated the highest reducing fat content in value was 80.53%. Using papain enzyme gave a significant effect on the DH of FPH, with value was 29.72-67.64%. Therefore, FPH obtained from different concentrations for 4 hours was chosen to characterize the antioxidant activity and bioactive peptide. Hydrolysis using 5% enzyme papain showed the highest antioxidant activity of DPPH, ABTS, and reducing power with values of 0.965 mg/mL, 0.495 mg/mL, and 0.415 absorbances, respectively. FPH possesses a molecular weight of 10.15-48.50 kDa. Functional groups detected were amides A, B, I, II, and III. Glycine, proline, and arginine became amino acids dominant of FPH. Based on the diversity of biopeptide compounds, several biological function candidates were detected, namely antioxidants, ACE inhibitors, and antifibrinolytics which have the potential to be used as nutraceutical products.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41617830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine Suitability Assessment for Mariculture: Combining GIS and AHP Technique in Dampier Strait Conservation Area, West Papua Province, Indonesia","authors":"Ichsan Suryo Wibowo, A. Mashar, N. T. Pratiwi, Fery Kurniawan","doi":"10.20473/jipk.v15i2.40172","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.40172","url":null,"abstract":"Highlight Research\u0000\u0000Mariculture is one of the activities that can pressure the aquatic environment.\u0000Mariculture activities are carried out by considering the sustainability aspects of environmentally friendly implementation.\u0000The suitability of the location for mariculture activities refers to the geographical and hydro–oceanographic conditions seen from the waters' physical, chemical, and biological parameters.\u0000The suitability index produced using multiple approaches (GIS-based AHP) can be used to determine the potential locations for mariculture development.\u0000\u0000 \u0000Abstract\u0000Potential mariculture activities in the Dampier Strait Conservation Area, which are indicated by the availability of water space and local commodities, can have a multiplier effect on socio-ecological aspects. Therefore, reasonable development steps are needed to carry out environmentally friendly aquaculture. This study aims to determine the potential use of water space for the development of mariculture activities based on the level of suitability using multiple approaches. Suitability analysis was carried out with Geographic Information System (GIS) based on the Analytical Hierarchy Process (AHP) by considering the area’s characteristics using several aspects seen from the geographical conditions and water quality (physical, chemical, and biological). Based on the suitability analysis results, the potential water space that can be used for mariculture development activities, namely seaweed and pearl oyster cultivation was 1,130.45 ha (0.49% of the overall space allocation 232,588.59 ha). A justification was then carried out to determine a suitable location in terms of its accessibility and environmental friendliness. The analysis has shown promising results in determining potential locations for developing mariculture activities for seaweed and pearl oyster commodities based on sustainable and environmentally friendly cultivation.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47163620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Acetic Acid Pretreatment on Hydro-extraction of Water-Soluble Collagen from Skin of Alaska Pollock (Theragra chalcogramma)","authors":"Patmawati, Aliffiansyah Rizky Ergion, L. Sulmartiwi, Raseetha Vani Siva Manikam, Dwitha Nirmala, Y. Waiprib, Sapta Wijayanti","doi":"10.20473/jipk.v15i2.41274","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.41274","url":null,"abstract":"Highlight Research\u0000\u0000The optimal condition for production of water-soluble collagen from Alaska pollock fish skin was the pretreatment with 0.15 M acetic acid followed by hydro-extraction at 40oC with 150 rpm for two hours.\u0000\u0000\u0000Higher acetic acid concentration at a pretreatment step enhanced the higher yield of water-soluble collagen via hydro-extraction process.\u0000Hydro-extraction was an environmentally friendly method for converting Alaska pollock fish skin to water-soluble collagen.\u0000Alaska pollock fish skin could be used as an alternative source for collagen production.\u0000\u0000Abstract\u0000To date, there is no information on skin of Alaska Pollock (Theragra chalcogramma) being used as a source for collagen. In order to produce water-soluble collagen from the skin of Alaska Pollock, a process known as hydro-extraction is utilized. This technique does not need a long extraction time or a large amount of chemical reagent. The purpose of this study was to investigate the effect of acetic acid pretreatment on hydro-extraction of water-soluble collagen from Alaska Pollock skin. The skin samples were pretreated using acetic acid at different concentrations (0.01 M; 0.05 M; 0.1 M, 0.15 M) for two hours at chilling temperature. The pretreated skin samples were further processed to produce water-soluble collagen using the hydroxy-extraction method. The obtained collagen was analyzed for proximate compositions, yield, and amino acids compositions with high performance liquid chromatography. It was found that the proximate compositions of the collagen products, specifically the protein content (75%) and fat content (1%), met the requirements of the Indonesian National Standard (SNI 8076:2014). The collagen yield ranged from 2.6 to 3.13%. The predominate amino acids in collagen were glycine, arginine, proline, glutamic acid, serine, and alanine. Pretreatment of skin sample with 0.15 M acetic acid resulted in the highest yield of water-soluble collagen (3.13%) and protein content (91.13%). The skin of Alaska Pollock fish could be used as an alternative raw material to produce water-soluble collagen for medical, pharmacy or food processing applications.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43328895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Length-weight Relationship and Condition Factors of Endemic Fish, <I>Lagusia micracanthus</I> Bleeker, 1860 (Pisces: Terapontidae) in Gilireng River, Wajo Regency, Indonesia","authors":"Neneng Rahayu Ningsih, Sharifuddin Bin Andy Omar, Abdul Haris, Muhammad Nur, Rakhma Fitria Larasati","doi":"10.20473/jipk.v15i2.40950","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.40950","url":null,"abstract":"Highlight Research Research on pirik endemic fish (Lagusia micracanthus) was first conducted in the Gilireng River. Based on the length-weight relationship, the growth type of pirik fish in the Gilireng River is isometric. The average size distribution, total length, and body weight of female fish were greater than those of male fish though not statistically significant. The Gilireng River water conditions are sufficient to support the growth of endemic pirik fish. Abstract Lagusia micracanthus or locally known as pirik fish and is the only endemic freshwater fish from the family Terapontidae. To date, published information related to biological aspects of this species in the Gilireng River, Arajang Village, Wajo Regency, is unavailable. This study aims to determine the length−weight relationship and condition factors of L. micracanthus fish populations in the Gilireng River. Fish sampling was performed once a month January 2022 to June 2022.The fish collected from Gilireng River comprised 69 male and 66 females during the study period. Pirik (L. micracanthus) in the Gilireng River demonstrated nearly an isometric growth pattern. Condition factor values ranged from 0.6455 to 4.3239, and the average condition factor values for female fish were smaller than male fish. The result of this study has contributed to the knowledge of the endemic pirik fish population in the Gilireng River that could assist fishery management scientists in conducting studies on the management of these fish.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136195891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back Matter Vol 15 No 1","authors":"Rozi S.Pi.,M.Biotech.","doi":"10.20473/jipk.v15i1.42979","DOIUrl":"https://doi.org/10.20473/jipk.v15i1.42979","url":null,"abstract":"","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48031405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Metter Vol 15 No 1","authors":"Rozi S.Pi.,M.Biotech.","doi":"10.20473/jipk.v15i1.42978","DOIUrl":"https://doi.org/10.20473/jipk.v15i1.42978","url":null,"abstract":"","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45749196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}