Enhancement of Astaxanthin Content in Mixed Culture of Dunaliella sp. and Azospirillum sp. under Light Intensity Treatment

Q4 Agricultural and Biological Sciences

Jurnal Ilmiah Perikanan dan Kelautan Pub Date : 2023-04-13 DOI:10.20473/jipk.v15i2.38596

Ria Amelia, Wulan Rahmani Akmal, E. Suyono

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Abstract

Highlight Research The growth rate was higher when Chlorella was induced by Azospirillum brasilense. The carbohydrate content increased when combined with Azospirillum brasilense. Determination of astaxanthin accumulation in Haematococcus pluvialis. Define condition favoring astaxanthin accumulation in Haematococcus pluvialis. Abstract Dunaliella sp. is a potential natural source of carotenoid pigments such as astaxanthin, β-carotene, and lutein. Dunaliella sp. can also accumulate other valuable products such as glycerol and protein. Another species is Azospirillum sp., which is known as microalgal growth-promoting bacteria. These bacteria are often cultured with microalgae because they contain indole-3-acetic acid, which can significantly increase the growth of microalgae. This study aimed to examine the pigment content in mixed culture of Dunaliella sp. and Azospirillum sp. after being treated with different light intensity treatment. In this study, Dunaliella sp. were cultivated by mixing with Azospirillum sp. under light stress. Two treatments were performed at light stress intensity of 3000 and 6000 lx. Light intensity is widely used as an important parameter in cultivation, which can affect the growth and production of microalgal biomass. In addition, spectrophotometric UV-Vis based measurement was conducted to investigate every single pigment content in all treatments under light stress for eight days. The number of cells, carotenoid pigments, and astaxanthin had increased significantly. Pigments of chlorophyll a and chlorophyll b also significantly increased at lower light treatments. Based on the results, the bacterium Azospirillum sp. and high light intensity significantly increased the growth and cell division of microalgae. Therefore, the combination of Azospirillum sp. and light stress intensity in microalgae cultivation could increase the growth and pigment of Dunaliella sp.

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光强处理对杜氏藻与偶氮螺旋藻混合培养虾青素含量的提高

亮点研究巴西固氮螺菌诱导小球藻生长速率较高。与巴西固氮螺菌组合后，碳水化合物含量增加。雨生红球藻中虾青素积累的测定。定义有利于雨生红球藻中虾青素积累的条件。杜氏藻是类胡萝卜素色素的潜在天然来源，如虾青素、β-胡萝卜素和叶黄素。杜氏藻还可以积累其他有价值的产物，如甘油和蛋白质。另一种是Azospirillum sp.，它被称为微藻生长促进细菌。这些细菌经常与微藻一起培养，因为它们含有吲哚-3-乙酸，可以显著增加微藻的生长。本研究旨在检测杜氏藻（Dunaliella sp.）和偶氮螺菌（Azospirillum sp.）混合培养物经不同光强处理后的色素含量。在光胁迫条件下，将杜氏藻（Dunaliella sp.）与偶氮螺菌（Azospirillum sp.）混合培养。在3000和6000lx的光应力强度下进行两次处理。光照强度是栽培中广泛使用的一个重要参数，它可以影响微藻生物量的生长和生产。此外，还进行了基于紫外-可见分光光度法的测量，以研究在光胁迫下8天的所有处理中的每一种色素含量。细胞数量、类胡萝卜素色素和虾青素显著增加。叶绿素a和叶绿素b的色素在较低的光照处理下也显著增加。结果表明，固氮螺菌和高光照显著促进了微藻的生长和细胞分裂。因此，在微藻培养中，氮螺菌与光胁迫强度的结合可以提高杜氏藻的生长和色素含量。

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