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Journal of Open Hardware最新文献

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Fully Automated Spotting Device for Thin-Layer Chromatography based on the Modification of a 3D Printer 基于 3D 打印机改装的薄层色谱全自动点样装置
Journal of Open Hardware Pub Date : 2024-07-05 DOI: 10.5206/joh.v8i1.18995
J. Neumaier, Anna-Lena Renz, Florian Menzel
{"title":"Fully Automated Spotting Device for Thin-Layer Chromatography based on the Modification of a 3D Printer","authors":"J. Neumaier, Anna-Lena Renz, Florian Menzel","doi":"10.5206/joh.v8i1.18995","DOIUrl":"https://doi.org/10.5206/joh.v8i1.18995","url":null,"abstract":"Column chromatography and the subsequent analysis of the collected fractions using thin-layer chromatography (TLC) are immensely popular and frequently used methods in organic chemistry. To analyze the fractions, they have to be spotted onto the TLC plates, which can be a time-consuming task. Therefore, we have developed an automatic spotting device based on a conversion of a 3D printer kit, which could be easily built for around 500 €. All parts come directly from the 3D printer kit, are 3D printed, or are commercially available, such as syringes and tubing. In this manuscript, we describe the design and use of the spotting device. In addition, we provide a detailed step-by-step assembly instruction and all CAD files for easy replication.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":" 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141673810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Low-Cost Electroporator for Genetically Modifying Social Amoeba Dictyostelium Discoideum 一种低成本的基因改造社会性变形虫盘状齿形变形虫的电穿孔器
Journal of Open Hardware Pub Date : 2023-05-08 DOI: 10.5334/joh.52
Michael C. Cauchy, Ali A. Khan, Y. Artemenko, D. Dunn
{"title":"A Low-Cost Electroporator for Genetically Modifying Social Amoeba Dictyostelium Discoideum","authors":"Michael C. Cauchy, Ali A. Khan, Y. Artemenko, D. Dunn","doi":"10.5334/joh.52","DOIUrl":"https://doi.org/10.5334/joh.52","url":null,"abstract":"The social amoeba Dictyostelium discoideum is a commonly used eukaryotic model organism for the study of cell division, chemotaxis, differentiation, phagocytosis, and other cellular processes. Electroporation is an effective and efficient method for delivering plasmid DNA into D. discoideum, an invaluable tool for studying intracellular processes. The technology is readily available but often prohibitively expensive. Although several custom-built electroporation devices have been developed, none deliver the specific 8.5kV/cm exponentially decaying waveform required for D. discoideum transformation. The present study examined whether a simple, inexpensive device can be built to produce this waveform through a simple resistor-capacitor (RC) circuit. A pulse generator RC circuit was built incorporating inexpensive electronic components and a 3D printed cuvette chamber. All four possible combinations of custom-built and commercial pulse generators and custom-built and commercial cuvette chambers were used to transform D. discoideum cells with a plasmid encoding green fluorescent protein (GFP). There were no significant differences in the number of surviving cells immediately following or 24 hours post-transformation between the systems. All combinations of custom-built and commercial systems achieved comparably high transformation efficiency shown by percent of cells expressing GFP six days after the transformation. Since the waveform-specific electroporation system we present here can be built by non-experts with easily obtainable materials and 3D printing, we envision this device to benefit investigators in areas with low research budgets and educators in multiple STEM fields.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"82 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131322061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OpenPodcar: An Open Source Vehicle for Self-Driving Car Research OpenPodcar:用于自动驾驶汽车研究的开源车辆
Journal of Open Hardware Pub Date : 2023-01-01 DOI: 10.5334/joh.46
Fanta Camara, Chris Waltham, Grey Churchill, Charles Fox
{"title":"OpenPodcar: An Open Source Vehicle for Self-Driving Car Research","authors":"Fanta Camara, Chris Waltham, Grey Churchill, Charles Fox","doi":"10.5334/joh.46","DOIUrl":"https://doi.org/10.5334/joh.46","url":null,"abstract":"OpenPodcar is a low-cost, open source hardware and software, autonomous vehicle research platform based on an off-the-shelf, hard-canopy, mobility scooter donor vehicle. Hardware and software build instructions are provided to convert the donor vehicle into a low-cost and fully autonomous platform. The open platform consists of (a) hardware components: CAD designs, bill of materials, and build instructions; (b) Arduino, ROS and Gazebo control and simulation software files which provide standard ROS interfaces and simulation of the vehicle; and (c) higher-level ROS software implementations and configurations of standard robot autonomous planning and control, including the move_base interface with Timed-Elastic-Band planner which enacts commands to drive the vehicle from a current to a desired pose around obstacles. The vehicle is large enough to transport a human passenger or similar load at speeds up to 15 km/h, for example for use as a last-mile autonomous taxi service or to transport delivery containers similarly around a city center. It is small and safe enough to be parked in a standard research lab and be used for realistic human-vehicle interaction studies. System build cost from new components is around USD7,000 in total in 2022. OpenPodcar thus provides a good balance between real world utility, safety, cost and research convenience.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135361955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
MACARONS: A Modular and Open-Sourced Automation System for Vertical Farming MACARONS:垂直农业的模块化和开源自动化系统
Journal of Open Hardware Pub Date : 2022-10-10 DOI: 10.5334/joh.53
V. Wichitwechkarn, Charles Fox
{"title":"MACARONS: A Modular and Open-Sourced Automation System for Vertical Farming","authors":"V. Wichitwechkarn, Charles Fox","doi":"10.5334/joh.53","DOIUrl":"https://doi.org/10.5334/joh.53","url":null,"abstract":"The Modular Automated Crop Array Online System (MACARONS) is an extensible, scalable, open hardware system for plant transport in automated horticulture systems such as vertical farms. It is specified to move trays of plants up to 1060mm x 630mm and 12.5kg at a rate of 100mm/s along the guide rails and 41.7mm/s up the lifts, such as between stations for monitoring and actuating plants. The cost for the construction of one grow unit of MACARONS is 144.96USD which equates to 128.85USD/m2 of grow area. The designs are released and meets the requirements of CERN-OSH-W, which includes step-by-step graphical build instructions and can be built by a typical technical person in one day at a cost of 1535.50USD. Integrated tests are included in the build instructions are used to validate against the specifications, and we report on a successful build. Through a simple analysis, we demonstrate that MACARONS can operate at a rate sufficient to automate tray loading/unloading, to reduce labour costs in a vertical farm.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134094740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
PhasIR: An Instrumentation and Analysis Software for High-throughput Phase Transition Temperature Measurements PhasIR:用于高通量相变温度测量的仪器和分析软件
Journal of Open Hardware Pub Date : 2021-12-27 DOI: 10.5334/joh.39
Jaime Rodriguez, Mara Politi, Sage Scheiwiller, Shrilakshmi Bonageri, Stuart Adler, David Beck, L. Pozzo
{"title":"PhasIR: An Instrumentation and Analysis Software for High-throughput Phase Transition Temperature Measurements","authors":"Jaime Rodriguez, Mara Politi, Sage Scheiwiller, Shrilakshmi Bonageri, Stuart Adler, David Beck, L. Pozzo","doi":"10.5334/joh.39","DOIUrl":"https://doi.org/10.5334/joh.39","url":null,"abstract":"","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123670876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Reconfigurable Mechanical Vibrations Laboratory Kit 可重构机械振动实验室套件
Journal of Open Hardware Pub Date : 2021-10-10 DOI: 10.5334/joh.40
Ian Ho, Ajay Harishankar Kumar, D. M. Harris
{"title":"Reconfigurable Mechanical Vibrations Laboratory Kit","authors":"Ian Ho, Ajay Harishankar Kumar, D. M. Harris","doi":"10.5334/joh.40","DOIUrl":"https://doi.org/10.5334/joh.40","url":null,"abstract":"Mechanical vibrations appear across a diverse range of applications, but nevertheless are unified by a core set of principles and phenomena. In this article, we describe a reconfigurable laboratory kit that is designed to be used as part of a first undergraduate course in mechanical vibrations. The kit was designed to be low-cost and allow for a series of laboratory experiments to be performed remotely. Using a common set of core hardware and software, five laboratory experiments are detailed and can be performed to supplement many of the major topics traditionally encountered in a first vibrations course. This accessible platform will readily enable further technical improvements and expansions, as well as the development of additional experiments and assignments.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129622067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TempSense: A Four-Channel Temperature Logger Based on a USB Bridge and CircuitPython TempSense:基于USB桥接和CircuitPython的四通道温度记录仪
Journal of Open Hardware Pub Date : 2021-07-20 DOI: 10.5334/joh.34
D. Soto
{"title":"TempSense: A Four-Channel Temperature Logger Based on a USB Bridge and CircuitPython","authors":"D. Soto","doi":"10.5334/joh.34","DOIUrl":"https://doi.org/10.5334/joh.34","url":null,"abstract":"Accurate temperature measurements are needed to monitor and reduce energy use and greenhouse gas emissions in building, industrial, and other processes. This paper describes a temperature sensor instrument, named TempSense, developed for classroom demonstrations of thermal phenomena. The instrument can monitor, record, and visualize data from multiple temperature sensors using a K-type amplifier and thermocouples. It has an accuracy of ±2 deg C from -200 to +700 deg C and an accuracy of ±6 deg C from –270 to +1372 deg C. The instrument uses a USB bridge and the Python language to interface with open-source thermocouple amplifiers without the need for byte-level programming. Its design allows novice experimenters to inspect the build and operation and even customize it to their needs. TempSense can be extended via Python libraries to provide internet communication, visualization, and analysis.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"205 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133004328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Open Source Hardware and Healthcare Collaborative Platforms: Common Legal Challenges 更正:开源硬件和医疗保健协作平台:共同的法律挑战
Journal of Open Hardware Pub Date : 2021-06-24 DOI: 10.5334/joh.37
E. Biasin, Erik Kamenjašević
{"title":"Correction: Open Source Hardware and Healthcare Collaborative Platforms: Common Legal Challenges","authors":"E. Biasin, Erik Kamenjašević","doi":"10.5334/joh.37","DOIUrl":"https://doi.org/10.5334/joh.37","url":null,"abstract":"This article details a correction to the article: Biasin, E. and Kamenjasevic, E., 2020. Open Source Hardware and Healthcare Collaborative Platforms: Common Legal Challenges. Journal of Open Hardware, 4(1), p. 7. DOI: https://doi.org/10.5334/joh.31","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"358 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122747067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
OptoGenie: an open-source device for the optogenetic stimulation of cells OptoGenie:用于细胞光遗传刺激的开源设备
Journal of Open Hardware Pub Date : 2021-01-27 DOI: 10.5334/JOH.32
Miranda Robbins, O. Siddiqui, T. Fuchsberger, Gemma Goodfellow, O. Paulsen, C. Kaminski, T. Euser, G. K. Schierle
{"title":"OptoGenie: an open-source device for the optogenetic stimulation of cells","authors":"Miranda Robbins, O. Siddiqui, T. Fuchsberger, Gemma Goodfellow, O. Paulsen, C. Kaminski, T. Euser, G. K. Schierle","doi":"10.5334/JOH.32","DOIUrl":"https://doi.org/10.5334/JOH.32","url":null,"abstract":"Optogenetics has revolutionised research in cell biology over the past 15 years, yet devices that can effectively stimulate cells using light are often costly and specifically designed for a single experimental set-up with little flexibility. Our novel ‘OptoGenie’ stimulation device can be conveniently transferred between cell culture incubators for long-term stimulation, electrophysiology rigs for patch-clamp recordings, and optical microscopes for fluorescence imaging of cells. The modular design of the device offers portability between these experimental set-ups, is low cost compared with commercial devices, and provides easy adjustment of the stimulation intensity and frequency. OptoGenie provides an open-source model made from proprietary parts such that researchers without experience with electronics and coding can easily purchase, assemble and customise for their experimental needs.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130936989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Cost-Effective Pulse Oximeter Designed in Response to the COVID-19 Pandemic 为应对COVID-19大流行而设计的具有成本效益的脉搏血氧仪
Journal of Open Hardware Pub Date : 2021-01-21 DOI: 10.5334/JOH.26
B. Metcalfe, Pejman Iravani, Jonathan Graham-Harper-Cater, R. Bowman, Julian Stirling, P. Wilson
{"title":"A Cost-Effective Pulse Oximeter Designed in Response to the COVID-19 Pandemic","authors":"B. Metcalfe, Pejman Iravani, Jonathan Graham-Harper-Cater, R. Bowman, Julian Stirling, P. Wilson","doi":"10.5334/JOH.26","DOIUrl":"https://doi.org/10.5334/JOH.26","url":null,"abstract":"This paper introduces the design of a small and cost-effective optical pulse oximeter, including a finger clip, sensor readout electronics, and associated firmware. The device is based on widely-available commercial optical sensors that are designed for the wearables market and measure the reflection of different wavelengths of light. This design was produced in response to a request from the Royal United Hospital in Bath UK, in light of the severe shortage of clinical pulse oximeters during the 2020 COVID-19 pandemic. Products based on this design have not been submitted for medical device approval; the design documents here should be considered purely for research, educational and development purposes.","PeriodicalId":176907,"journal":{"name":"Journal of Open Hardware","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128708115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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