Journal of virological methods最新文献

{"title":"Comparison of plant- and mammalian cell-produced human papillomavirus pseudovirions","authors":"Albertha R. van Zyl ,&nbsp;Sarah Lindsay ,&nbsp;Georgia Schäfer ,&nbsp;Edward P. Rybicki ,&nbsp;Inga I. Hitzeroth","doi":"10.1016/j.jviromet.2025.115215","DOIUrl":"10.1016/j.jviromet.2025.115215","url":null,"abstract":"<div><div>High-risk human papillomaviruses (HPVs) are the primary etiological agents of cervical, anal and oropharyngeal cancers. While existing vaccines are effective in preventing infection, their impact in low-and middle-income countries (LMICs) is limited by type coverage, high costs and uptake. To address this gap, there is a critical need for next-generation vaccines that are both regionally tailored and cost-effective, along with efficient and accessible tools for evaluating their efficacy. HPV pseudovirions (PsVs), which encapsidate a reporter plasmid, are widely used in pseudovirion-based neutralisation assays (PBNAs) and <em>in vivo</em> murine models to assess vaccine-induced immunity – and have potential for use as DNA vaccine delivery systems. Traditionally, PsVs are produced in mammalian cells, which remain the gold standard due to their high infectivity and structural fidelity. However, recent studies have demonstrated the feasibility of producing PsVs in plants, a platform that offers lower infrastructure and reagent costs, scalability, and biosafety advantages. Although plant-derived PsVs have shown promise in PBNAs, their performance in <em>in vivo</em> models had not been evaluated prior to this study. Here, we compared mammalian cell-derived PsVs encapsidating either Gaussia or firefly luciferase reporter plasmids and found that firefly luciferase provided more consistent and robust signals in both <em>in vitro</em> and <em>in vivo</em> assays. Building on this, we generated PsVs encapsidating the firefly luciferase gene using both mammalian and plant expression systems, and assessed their infectivity. While plant-derived PsVs were capable of infecting HeLa cells and mice in a cervicovaginal challenge model, mammalian-derived PsVs exhibited significantly higher infectivity overall. These findings represent the first demonstration of <em>in vivo</em> infectivity of plant-produced HPV PsVs and highlight their potential as a cost-effective alternative for immunogenicity testing and potentially as vaccines. Although further optimization is needed, particularly in capsid assembly and purification, plant-based PsV production holds promise for expanding access to HPV research tools and supporting vaccine development in resource-limited settings.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115215"},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of rapid and simple FCoV RNA detection systems using RT-PCR and RT-RPA combined with STH-PAS to diagnose FIP in cats","authors":"Tomoyoshi Doki,&nbsp;Misaki Ohno,&nbsp;Mihoko Kaku,&nbsp;Saori Odani,&nbsp;Kaito To,&nbsp;Tomomi Takano","doi":"10.1016/j.jviromet.2025.115214","DOIUrl":"10.1016/j.jviromet.2025.115214","url":null,"abstract":"<div><div>Feline infectious peritonitis (FIP) is a fatal disease in cats that is caused by feline coronavirus (FCoV). FCoV RT-qPCR is widely used to diagnose FIP due to its high sensitivity and ability to quantify FCoV RNA. However, its convenience is limited by the need for expensive equipment and/or processing at external laboratories. We herein developed two rapid and simple FCoV RNA detection systems: one combining conventional RT-PCR with the Single Tag Hybridization-Printed Array Strip (STH-PAS) method (the RT-PCR and STH-PAS system) and the other combining RT-RPA, an isothermal nucleic acid amplification method, with STH-PAS (the RT-RPA and STH-PAS system). Evaluations using FCoV RNA standards showed that the limit of detection for the RT-PCR and STH-PAS system was 10^4.2 copies/reaction, while that for the RT-RPA and STH-PAS system was 10⁴ copies/reaction. The clinical performance of these systems was also examined using clinical samples from cats suspected of having FIP and compared to the conventional FCoV RT-qPCR genetic test. The results obtained showed a sensitivity of 66.7 % (95 % CI: 41.0–86.7) and specificity of 100 % (95 %CI: 9.4–100). These systems are a faster and simpler alternative to conventional methods, suggesting their potential in point-of-care testing in veterinary clinics.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115214"},"PeriodicalIF":2.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a one-step RT-qPCR assay for the detection of Citrus concave gum-associated virus in apples","authors":"H.S. Bennypaul ,&nbsp;S. St-Jacques ,&nbsp;Isabella Schmidt ,&nbsp;J. Nakata ,&nbsp;D.S. Sanderson","doi":"10.1016/j.jviromet.2025.115211","DOIUrl":"10.1016/j.jviromet.2025.115211","url":null,"abstract":"<div><div>The yield and quality of apples are affected by diseases caused by bacteria, fungi, viruses, and virus-like organisms. Several viruses are known to cause or be associated with diseases of economic importance which impact apple production. Citrus concave gum-associated virus (CCGaV; order <em>Bunyavirales</em>, family <em>Phenuiviridae</em>, genus <em>Coguvirus</em>) is a negative-stranded RNA virus, with a bipartite genome of negative-stranded RNA1 and an ambisense RNA2. In recent years, CCGaV was identified in apple germplasm collections in a number of countries. Although identified in apple trees showing decline, CCGaV could not be implicated in disease causation due to the presence of other coinfecting viruses. As a precautionary measure, virus elimination programs have included CCGaV in the list of viruses that should be eliminated from the propagative material before distribution to its stakeholders. Detection of tree fruit viruses in woody hosts is difficult as compared to annual crops, due to the low titer, irregular distribution, and the occurrence of mixed infections. Sensitive and specific virus detection methods are critical part of virus elimination programs, first to identify virus infected plants that need to be treated and then to certify virus-free status of after virus elimination treatments. In this study, an RT-qPCR assay was developed for sensitive and specific detection of CCGaV in apples.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115211"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards efficient and targeted sampling of primary respiratory diseases from wastewater in congregate settings for seniors: Empowering high-risk demographics with prospective health threat data","authors":"Erin N. Morrison ,&nbsp;Matthew Harnden ,&nbsp;Emma Boisvert ,&nbsp;Audrey E. Wilson ,&nbsp;Thomas Piggott ,&nbsp;Carolyn Pigeau ,&nbsp;Christopher J. Kyle","doi":"10.1016/j.jviromet.2025.115212","DOIUrl":"10.1016/j.jviromet.2025.115212","url":null,"abstract":"<div><div>Respiratory disease outbreaks with overlapping symptomology in long-term care and congregate living facilities can have disproportionately negative impacts on the health and well-being of residents. Wastewater surveillance of SARS-CoV-2 demonstrated efficacy as an early outbreak warning for congregate facilities allowing for the implementation of effective non-pharmaceutical interventions. Assays that concomitantly target multiple respiratory pathogens exist for clinical diagnosis; however, challenges remain in the implementation of similar multi-pathogen surveillance from wastewater in terms of specificity, sensitivity and connections to clinical data. Herein, RT-qPCR multiplex assays were developed, combining detection of SARS-CoV-2, influenza and respiratory syncytial virus (RSV) into a single assay, reducing time and cost per sample. Data were analyzed in context of single pathogen detection sensitivity and known outbreaks at 1 long-term care facility, 4 retirement homes and 1 community site in Peterborough, ON, Canada. Analyses focused on 8 outbreak periods (SARS-CoV-2 (6); influenza (1); RSV (1)), 2 suspected influenza outbreaks, and parallel respiratory outbreaks. Wastewater signals for pathogens correlated with reported outbreak periods at facilities, while relative sensitivity was reduced, multiplex assay results had comparable signal trends to that of single pathogen assays. Among SARS-CoV-2 outbreaks, wastewater signals were detected ∼ 3–4 days prior to outbreaks. For influenza and RSV outbreaks, consistent wastewater signals were detected 3 and 12 days prior, respectively. A multiplexed assay approach allowed for identification of parallel respiratory pathogen outbreaks with overlapping symptomology. These findings support wastewater surveillance and efficiencies of multiplexing respiratory virus detection without losing signal detection for ongoing reduced-cost monitoring programs.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115212"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of the Coxsackievirus A24 variant in the Acute Hemorrhagic Conjunctivitis outbreak (2023) in Islamabad, Pakistan through metagenomic next generation sequencing","authors":"Syed Adnan Haider ,&nbsp;Zunera Jamal ,&nbsp;Muhammad Ammar ,&nbsp;Rabia Hakim ,&nbsp;Babak Afrough ,&nbsp;Anila Kreku ,&nbsp;Leena Inamdar ,&nbsp;Muhammad Salman ,&nbsp;Massab Umair","doi":"10.1016/j.jviromet.2025.115213","DOIUrl":"10.1016/j.jviromet.2025.115213","url":null,"abstract":"<div><div>Pakistan experienced a significant outbreak of Acute Hemorrhagic Conjunctivitis (AHC) in 2023. To identify the cause, in the absence of targeted diagnostic tests, the National Institute of Health, Islamabad, studied 15 conjunctivitis patients from Islamabad in September 2023. Metagenomic Next Generation Sequencing (mNGS) was performed on 10 samples collected within 48 h of symptom onset. The human Coxsackievirus A24 variant (CV-A24v), genotype IV, was detected in three samples. Phylogenetic analysis showed ∼99 % similarity with recent strains from China and 94 % similarity with the 2015 France outbreak. Mutation analysis revealed mostly non-synonymous substitutions, particularly in the VP1 region (n = 14), and differences in 2 C and 3D regions of nonstructural proteins. Comparison with 2005 Pakistan outbreak sequences showed divergence in the VP1 region, with two distinct mutations (\"L16I\" and \"L25H\"), with L16I being a rare mutation observed only in strain from China and India in 2023. Structural modeling of VP1 proteins indicated conformational differences between the 2023 and 2005 strains, suggesting potential impacts on viral infectivity and immune escape. These findings indicate the reemergence of CV-A24v in Pakistan and highlight the importance of adaptable diagnostic strategies to respond to emerging infectious threats.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115213"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel plate reader-based antibody-dependent enhancement (ADE) assay for orthoflavivirus infections","authors":"Yannis Aklil ,&nbsp;Hiroto Takeuchi ,&nbsp;Gabriel Gonzalez ,&nbsp;Atsuko Inoue ,&nbsp;Kaito Maeda ,&nbsp;Shintaro Kobayashi ,&nbsp;Yukari Itakura ,&nbsp;Shinji Saito ,&nbsp;Anavaj Sakuntabhai ,&nbsp;Michihito Sasaki ,&nbsp;William W. Hall ,&nbsp;Hirofumi Sawa ,&nbsp;Yasuko Orba ,&nbsp;Koshiro Tabata","doi":"10.1016/j.jviromet.2025.115210","DOIUrl":"10.1016/j.jviromet.2025.115210","url":null,"abstract":"<div><div>Antibody-dependent enhancement (ADE) is one of the mechanisms associated with severe clinical outcomes in infections caused by certain viruses, including dengue virus (DENV). Several ADE assay systems have been established, including flow cytometric assays using live viruses, enzyme-linked immuno-sorbent assay (ELISA) for the detection of viral NS1, and luciferase reporter gene assays. Among these, the flow cytometric assay is the most commonly used to evaluate ADE activity; however, it has limitations such as high operational costs due to fixation and immunostaining procedures, as well as a long analysis time. Fluorescent protein-expressing single-round infectious particles (SRIPs) enables label-free detection of ADE activity, but the flow cytometric procedure still requires a long analysis time. In this study, to simplify and expedite the ADE assay using enhanced green fluorescent protein (EGFP)-expressing SRIPs, we developed a plate reader-based ADE assay as an alternative to the conventional flow cytometry-based method. To evaluate effectiveness of this assay, we measured ADE activities in K562 cells induced by pan-orthoflavivirus 4G2 and pan-dengue 4E11 monoclonal antibodies (mAbs) using both flow cytometric assays using live viruses and plate-reader-based EGFP-expressing SRIPs assays. The results showed strong correlations between the two different ADE assays with R² values of 0.92 for 4G2 mAb and 0.94 for 4E11 mAb (Pearson correlation coefficients). In summary, this newly established assay offers a high-throughput and cost-effective method for comprehensive characterization of the relationship between vaccine- or infection-induced antibodies and ADE in orthoflavivirus infections.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115210"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144371303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA","authors":"Xin-Yu Lian ,&nbsp;Xiu-Hua Wang ,&nbsp;Chen Li ,&nbsp;Qing-Li Zhang ,&nbsp;Ruo-Xuan Lu ,&nbsp;Zi-Yue Gou ,&nbsp;Hua Xu ,&nbsp;Mei-Feng Wang ,&nbsp;Peng Jia ,&nbsp;Bing Yang","doi":"10.1016/j.jviromet.2025.115209","DOIUrl":"10.1016/j.jviromet.2025.115209","url":null,"abstract":"<div><div>Finders Technology Associates (FTA) cards offer a streamlined solution for the storage, transportation, and extraction of samples. In this study, we assessed the diagnostic efficacy of IHHNV DNA sample storage on FTA cards following simulated transportation under varying conditions (-20°C, 4°C, 25°C, 37°C) over a 60d period. By comparing two elution methods, we determined that optimal results were achieved by performing three elutions with FTA purification reagent at room temperature, followed by two elutions with TE buffer, each lasting 5 min, with a 200 μL elution volume. After the final elution, the membrane was air-dried at room temperature for 1 h before real-time PCR analysis. Our findings revealed that there was no statistically significant difference in nucleic acid preservation with respect to storage temperature and time (<em>P</em> &gt; 0.05). This suggests that nucleic acids stored on FTA cards remained stable under all tested temperature conditions for a minimum of 2 months. For on-site IHHNV monitoring, we analyzed shrimp homogenate samples. Our results demonstrated that eluting and extracting nucleic acid directly from shrimp tissue using FTA cards yielded results comparable in magnitude to conventional reagent kits when analyzed using real-time PCR. This underscores the equivalence of these two methods for nucleic acid extraction. FTA cards prove to be an effective tool for storing and detecting IHHNV DNA samples, offering a convenient and environmentally friendly solution. This method can be routinely employed and easily transported via standard postal systems, making it particularly advantageous for cross-border transportation.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115209"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of virus analytical methods on the estimation of virus reductions by ultrafiltration","authors":"Kaitlyn J. Chung ,&nbsp;Abigail G. Albright ,&nbsp;Dave W. Goad ,&nbsp;Andrew E. Page ,&nbsp;Bianca M. Souza-Chaves ,&nbsp;Andrea Achilli ,&nbsp;Michael J. Hegetschweiler ,&nbsp;Lester M. Shulman ,&nbsp;Walter Q. Betancourt","doi":"10.1016/j.jviromet.2025.115208","DOIUrl":"10.1016/j.jviromet.2025.115208","url":null,"abstract":"<div><div>This study evaluated the influence of analytical methods on the quantitative estimation of viruses in recycled waters and their reductions by an ultrafiltration (UF) engineering-scale system. Adenoviruses, crAssphage, <em>Pepper mild mottle virus</em> (PMMoV), culturable male-specific and somatic coliphages selected through a comparative quantitative analysis were evaluated in UF feed and UF permeate using a combination of analytical methods for virus concentration and absolute quantification of virus genomes and infectious coliphages by digital PCR and plaque assays, respectively. Both methods of virus concentration, centrifugal ultrafiltration and the InnovaPrep CP Select™ Concentrating Pipette (CP), demonstrated similar performance for the recovery of viruses from UF feed and UF permeate, however the CP approach allowed more rapid sample filtration than centrifugal ultrafiltration. Procedures commonly applied for the detection of viruses in water generated different quantitative outcomes that were influenced by the type of virus and other natural sources of variation associated with UF feed and UF permeate water. These quantitative outcomes in virus measurements led to highly variable estimations of virus reductions ranging from no apparent reduction to a maximum of 2-log₁₀. These results indicate that more accurate estimations of virus levels and reductions in water purification processes require adjustments in analytical procedures under the wide variety of water characteristics associated with each stage of treatment. A thorough understanding of the interactions between structural features of natural occurring viruses in recycled waters and water quality characteristics is crucial for improving virus recoveries and for the assessment of membrane-based treatment systems as barriers against microbial targets of environmental and public health concern.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115208"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and application of infectious cDNA clone, subgenomic replicon and packaging system for Zika virus and Dengue virus","authors":"Yu He ,&nbsp;Yibin Tang ,&nbsp;Xiaoli Wang ,&nbsp;Zhen Wu ,&nbsp;Tao Wang ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Dekang Zhu ,&nbsp;Mafeng Liu ,&nbsp;Xinxin Zhao ,&nbsp;Qiao Yang ,&nbsp;Ying Wu ,&nbsp;Shaqiu Zhang ,&nbsp;Juan Huang ,&nbsp;Bing Tian ,&nbsp;Xumin Ou ,&nbsp;Di Sun ,&nbsp;Anchun Cheng ,&nbsp;Shun Chen","doi":"10.1016/j.jviromet.2025.115207","DOIUrl":"10.1016/j.jviromet.2025.115207","url":null,"abstract":"<div><div>Flaviviruses pose a significant global health threat due to their rapid spread and potential to cause severe clinical manifestations. Comprehending the mechanisms of replication of these pathogens and developing effective antiviral strategies are essential for combating these pathogens. In the present study, full-length infectious cDNA clones were generated for Zika virus (ZIKV) and Dengue virus (DENV), respectively. Recombinant viruses were successfully produced by transfecting cDNA clone plasmids. Using the infectious clone, ZIKV and DENV subgenomic replicons were also generated, which lack the prM-E gene and instead expressing a luciferase or fluorescent marker (OXGFP or mCherry). The replicons exhibited efficient replication in BHK-21 cells. Through the utilization of ZIKV and DENV-2 replicons that express luciferase, three potential antiviral agents were identified. These agents demonstrated activity against DENV-2 and ZIKV, while not inducing significant cytotoxic effects. This demonstrates the significance of these replicons in the screening of antiviral agents. Moreover, DENV-2 and ZIKV single-round infectious particles (SRIPs) were produced by co-transfecting packaging plasmids and replicons into BHK-21 cells. The packaging assay demonstrated that flaviviruses can utilize the prM-E protein from other species to generate SRIPs. The specific binding of the nucleocapsid (NC) to the prM-E protein varies among different flaviviruses. The reverse genetics tools established in this study will facilitate research on DENV-2 and ZIKV virus replication and the development of antiviral medications targeting these two arboviruses.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115207"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of Zika virus using a colloidal gold nanoparticle-based immunosensor and Fourier-transform infrared spectroscopy","authors":"Mario Alberto Cuapa-González ,&nbsp;Gerardo Santos-López ,&nbsp;Hugo Martínez-Gutiérrez ,&nbsp;Zeus Saldaña-Ahuactzi ,&nbsp;Francisco Severiano-Carrillo ,&nbsp;Orlando Zaca-Morán ,&nbsp;Abdu Orduña-Díaz ,&nbsp;Marlon Rojas-López","doi":"10.1016/j.jviromet.2025.115206","DOIUrl":"10.1016/j.jviromet.2025.115206","url":null,"abstract":"<div><div>Zika virus (ZIKV) is an arthropod-borne virus that has spread worldwide and is the etiological agent of Zika fever, a disease of global concern distinguished by mild symptoms and recently linked to a congenital syndrome in neonates. Microcephaly, hydrocephalus, ocular deformations, and meningoencephalitis characterize this disease. The plaque assay is the gold standard used in scientific research laboratories to quantify the virus titer on a sample and assess their relationship with the infectious dosage. However, it requires costly infrastructure and specialized technical training. In this study, a nano-immunosensor based on gold nanoparticles was developed to detect Zika virus using FTIR spectroscopy. Three calibration curves were established to estimate ZIKV titers by measuring the intensity of absorption bands associated with functional groups in viral samples at different concentrations. The detection sensitivity ranged from 5 × 10⁴ to 5 × 10⁷ PFU/mL for the lipid group (C<img>O, 1750 cm⁻¹), 5 × 10 ³ to 5 × 10⁷ PFU/mL for the protein group (amide I, 1680 cm⁻¹), and 5 × 10⁵ to 2.2 × 10⁸ PFU/mL for the carbohydrate group (C-O, 860 cm⁻¹). The proposed method highlights the potential of the colloidal immunosensor for the quantification of the Zika virus.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115206"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}