Journal of Toxicological Sciences最新文献_第4页

Effect of the barrier function of stratum corneum and viable epidermis and dermis on the skin concentration of topically applied chemicals. 角质层、活表皮和真皮层屏障功能对外敷化学药品皮肤浓度的影响。

IF 1.8 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.187

Hiroaki Todo, Takeshi Oshizaka, Syuuhei Komatsu, Kenji Sugibayashi

{"title":"Effect of the barrier function of stratum corneum and viable epidermis and dermis on the skin concentration of topically applied chemicals.","authors":"Hiroaki Todo, Takeshi Oshizaka, Syuuhei Komatsu, Kenji Sugibayashi","doi":"10.2131/jts.50.187","DOIUrl":"10.2131/jts.50.187","url":null,"abstract":"Three-dimensional cultured skin (3D skin) models have been utilized for in vitro skin permeation tests to evaluate the skin permeation rate and local effects (efficacy and toxicity) of applied chemicals, particularly from the perspective of the 3Rs (reduction, replacement, refinement) approach. The steady-state concentration of applied chemicals at different depths in the viable epidermis and dermis (VED) is affected by their skin permeation parameters, such as permeability coefficient (Kp) and partition coefficient (K) from the donor solution to the skin of the chemicals. In the present study, the steady-state concentration of chemicals in the VED of EpiDerm 606X (EpiDerm) as representative of a 3D skin model were compared with hairless rat skin. The VED concentrations of chemicals in EpiDerm were higher than those in hairless rat skin when a model hydrophilic compound, antipyrine, and a model lipophilic compound, flurbiprofen, were applied, suggesting that the barrier functions of the VED against the whole skin were higher in EpiDerm than in hairless rat skin. When an ester compound, ethyl nicotinate, was applied, the VED concentration of nicotinic acid, a metabolite of ethyl nicotinate, was lower in EpiDerm than in hairless rat skin. These differences in the VED concentrations of applied chemicals might be related to false-positives and -negatives of topical effects evaluated with 3D skin models. It is important to pay particular attention to differences in VED concentration in 3D skin models and real skin when evaluating local efficacy and toxicity using 3D skin models.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 4","pages":"187-198"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic liquid cytotoxicity in mouse macrophage J774.1 cells: Effects of alkyl chain length, cation core, and anion type. 离子液体对小鼠巨噬细胞J774.1细胞的细胞毒性：烷基链长度、阳离子核和阴离子类型的影响。

IF 1.5 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.483

Takahito Nishiyama, Ryousuke Suda, Toshio Hirokawa, Kyoko Fujita, Kimiyoshi Ichida, Satoshi Yamaori

{"title":"Ionic liquid cytotoxicity in mouse macrophage J774.1 cells: Effects of alkyl chain length, cation core, and anion type.","authors":"Takahito Nishiyama, Ryousuke Suda, Toshio Hirokawa, Kyoko Fujita, Kimiyoshi Ichida, Satoshi Yamaori","doi":"10.2131/jts.50.483","DOIUrl":"https://doi.org/10.2131/jts.50.483","url":null,"abstract":"Ionic liquids (ILs) are salts with melting points below 100°C. These materials are promising novel solvents in organic reactions, as new electrolytes, and in protein stabilization, able to refold enzymes and aid in drug discovery. IL properties are strongly influenced by the types of their constituent cations and anions. To date, many ILs with unique solvent properties not found in water or organic solvents are reported. However, IL toxicity evaluations often focus on trends associated with widely used imidazolium cations. Consequently, knowledge regarding the toxicity of individual ionic structures and their combinations is limited. This study investigated alkylphosphonium and alkylammonium cation derivatives, evaluating their cytotoxicity against mouse macrophage J774.1 cells using dihydrogen phosphate ([dhp]) or bromide (Br) ions as anions. Experiments were conducted using alkylphosphonium cation derivatives ((C4H9)3RP+, [P444R]) with varying alkyl chain lengths (R: 4; C4H9, 8; C8H17, and 12; C12H25) and on tetrabutylammonium cation derivatives [N4444]. This study revealed that [P44412][dhp] yielded the highest toxicity, which decreased with shorter alkyl chains. A similar trend was observed with Br as the anion. For [P444R], anion variation did not significantly affect toxicity. Comparing [P4444][dhp] and [N4444][dhp], the phosphonium cation showed lower ED50 values, indicating higher toxicity. This tendency persisted when Br was used as the anionic species. In summary, for the ILs evaluated, the quaternary cationic species of the IL effects the greatest influence on IL toxicity.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 9","pages":"483-491"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation and classification of chemically-induced zebrafish malformations for the ICH S5 (R3) guideline: an atlas for zebrafish teratogenesis. ICH S5 （R3）指南中化学诱导斑马鱼畸形的变异和分类：斑马鱼畸形图谱。

IF 1.5 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.431

Kanako Mori, Yoshinobu Aoki, Mai Hayashi, Wataru Sugimoto, Mizuho Ono, Saaya Umekita, Tatsuhiro Niino, Tomonori Ebata, Fumito Mikashima, Kazushige Maki, Toshio Tanaka, Hiromi Hirata, Hajime Kojima

{"title":"Variation and classification of chemically-induced zebrafish malformations for the ICH S5 (R3) guideline: an atlas for zebrafish teratogenesis.","authors":"Kanako Mori, Yoshinobu Aoki, Mai Hayashi, Wataru Sugimoto, Mizuho Ono, Saaya Umekita, Tatsuhiro Niino, Tomonori Ebata, Fumito Mikashima, Kazushige Maki, Toshio Tanaka, Hiromi Hirata, Hajime Kojima","doi":"10.2131/jts.50.431","DOIUrl":"https://doi.org/10.2131/jts.50.431","url":null,"abstract":"Exposure of embryos or fetuses to harmful substances, such as teratogens, can result in embryonic or fetal death and a wide range of malformations. Zebrafish models have emerged as a valuable tool for assessing developmental toxicity and safety profiles of chemical compounds. Our previous research demonstrated that zebrafish larvae exhibit developmental abnormalities that mirror those observed in mammalian studies for more than 80% of the known Reference Compounds listed in the ICH S5 (R3) guideline. In this study, we presented high-resolution images depicting pharmaceutical-induced malformations across multiple anatomical regions, including the body axis, somites, notochord, fins, head, eyes, otoliths, jaw, heart, abdomen, and whole body. Frequent co-occurrence of specific defects, such as body axis and notochord malformations, was observed as described previously. Some physiological and morphological features, including heartbeat rate alterations and swim bladder inflation, were deemed dispensable for MEFL testing in zebrafish. Reproducibility was confirmed through inter-laboratory testing conducted both within our group and by other groups, supporting the reliability of zebrafish MEFL testing as an alternative approach in line with ICH S5 (R3).","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 8","pages":"431-444"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activator protein-1 mediates blue light-induced phototoxicity in retinal pigment epithelial cells. 激活蛋白-1介导蓝光诱导视网膜色素上皮细胞的光毒性。

IF 1.5 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.569

Jae Rim Lee, Myeong Ryeo Kim, Kwang Won Jeong

{"title":"Activator protein-1 mediates blue light-induced phototoxicity in retinal pigment epithelial cells.","authors":"Jae Rim Lee, Myeong Ryeo Kim, Kwang Won Jeong","doi":"10.2131/jts.50.569","DOIUrl":"https://doi.org/10.2131/jts.50.569","url":null,"abstract":"Age-related macular degeneration is a leading cause of vision loss and is characterized by the accumulation of drusen in the retinal pigment epithelium. N-retinylidene-N-retinylethanolamine (A2E), a major component of drusen, induces phototoxicity upon exposure to blue light. Given that blue light activates the MAPK pathway and triggers apoptosis, the present study aimed to determine the role of signaling via the activator protein-1 (AP-1) transcription factor in A2E-laden ARPE-19 cells. RNA-sequencing identified significant upregulation of the UV response and p53 pathways. In silico analysis predicted that JUN was a key upstream transcriptional regulator, and experimental validation confirmed increased JUN phosphorylation and AP-1 target gene expression upon blue light exposure. Furthermore, blue light treatment decreased BCL2 and increased BAX protein levels, thereby promoting apoptosis via caspase activation and PARP cleavage, as also confirmed by flow cytometry. These findings suggest that blue light induces apoptosis via JUN, which activates AP-1 in A2E-laden ARPE-19 cells. The present study provides new insights into the molecular mechanisms underlying blue light-induced retinal damage and its potential contribution to the progression of age-related macular degeneration.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 10","pages":"569-576"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of putrefactive amine and ammonia concentrations around decomposed corpses. 腐尸周围腐胺和氨浓度的测定。

IF 1.8 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.75

Hiroaki Sato, Takahiro Umehara, Satoshi Kimura, Toshiko Tanaka, Sang-Eun Kim

{"title":"Determination of putrefactive amine and ammonia concentrations around decomposed corpses.","authors":"Hiroaki Sato, Takahiro Umehara, Satoshi Kimura, Toshiko Tanaka, Sang-Eun Kim","doi":"10.2131/jts.50.75","DOIUrl":"10.2131/jts.50.75","url":null,"abstract":"The surface of a rotting corpse is covered with liquid decomposition products that have flowed out of the body that include putrefactive amines produced via putrefaction and decarboxylation reactions of proteins. Ammonia generated by deamination is also present around the corpse as a liquid or gas. As these putrefactive substances are toxic to humans, we attempted to measure the concentration of putrefactive substances in decomposed corpses in this study. Liquid putrefaction products were collected from the surface of a corpse, and the concentrations of putrefactive amines such as histamine, tyramine, phenethylamine, and tryptamine were analyzed by LC-MS/MS. Ammonia in the liquid and air around the corpse was also measured. Putrefactive amines and ammonia were present on all corpse surfaces. The highest concentrations and postmortem days in parentheses were as follows: histamine 2.26 mg/g (15 days), tyramine 1.77 mg/g (16 days), phenethylamine 4.90 mg/g (24 days), tryptamine 1.58 mg/g (17 days) and ammonia 25.6 mg/g (24 days postmortem). The highest concentration of ammonia in the air was 1310 ppm at 24 days postmortem. The ammonia level in the air around a corpse is toxic to humans. Inhalation of putrefactive amines and ammonia can cause chemical irritation to the respiratory tract and the skin and damage the mucous membrane of the eye. Oral ingestion can also cause poisoning symptoms such as blood pressure changes and headaches. Adequate protection against putrefactive substances is required when in contact with decaying corpses.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 2","pages":"75-81"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of postmortem product of amlodipine decomposition by hemoglobin with LC-Q-Orbitrap-MS. 用LC-Q-Orbitrap-MS鉴别氨氯地平尸体分解产物。

IF 1.8 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.325

Yoshikazu Yamagishi, Hiroyuki Inoue, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra

{"title":"Identification of postmortem product of amlodipine decomposition by hemoglobin with LC-Q-Orbitrap-MS.","authors":"Yoshikazu Yamagishi, Hiroyuki Inoue, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra","doi":"10.2131/jts.50.325","DOIUrl":"https://doi.org/10.2131/jts.50.325","url":null,"abstract":"Amlodipine (AM), a dihydropyridine calcium channel blocker, is frequently prescribed for hypertension in the clinical setting. Because AM has been detected in various lethal poisoning and suicide cases, it is important to determine its precise concentration in postmortem blood to serve as definitive evidence of death by intoxication. However, blood AM concentration at autopsy frequently differs from that at the time of death. In this study, we found that AM undergoes dehydrogenation by H2O2 at temperatures ranging from 4 to 45ºC. Mass spectra measured by quadrupole-Orbitrap mass spectrometry hyphenated with liquid chromatography showed the generation of 3-ethyl 5-methyl 2-((2-aminoethoxy)methyl)-4-(2-chlorophenyl)-6-methylpyridine-3,5-dicarboxylate (AM-PDP-1) in H2O2 and Hb/H2O2 reaction solutions incubated with AM and in postmortem blood of persons who died of drowning, fire, disease, drug poisoning, CO poisoning, traumatic shock, falling, or choking, after intentional ingestion of AM. AM-PDP-1 is the novel postmortem degradation compound of AM in blood. This compound in the Hb/H2O2 reaction solution was more stable than AM at 4-45ºC. These results show that AM-PDP-1, formed by H2O2-mediated postmortem AM decomposition, is a potential biomarker to correct for AM concentration in postmortem blood.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 7","pages":"325-332"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral exposure to polystyrene nanoplastics induces anxiety-like behavior and cognitive deficit accompanied with alteration of neuroimmune markers in rats. 口服聚苯乙烯纳米塑料可诱导大鼠焦虑样行为和认知缺陷，并伴有神经免疫标记物的改变。

IF 1.5 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.507

Tin-Tin Win-Shwe, Chaw Kyi-Tha-Thu

{"title":"Oral exposure to polystyrene nanoplastics induces anxiety-like behavior and cognitive deficit accompanied with alteration of neuroimmune markers in rats.","authors":"Tin-Tin Win-Shwe, Chaw Kyi-Tha-Thu","doi":"10.2131/jts.50.507","DOIUrl":"https://doi.org/10.2131/jts.50.507","url":null,"abstract":"Microplastic (MP) pollution has become a global environmental issue, but its potential health effect remains unknown. We aimed to investigate the effect of oral administration of polystyrene nanoplastics (PSNPs) on brain functions and behaviors. Five-week-old Sprague Dawley male rats were given 50 nm PSNPs orally at doses of 10 or 50 mg/kg thrice per week for four weeks. At 9-week-old after completion of oral exposure, novel object recognition test and open field test were performed. The hippocampus from each rat was collected to detect neurological, immunological, and antioxidative stress markers using ELISA, real-time RT-PCR and immunohistochemical analyses. High-dose PSNP-treated rats showed decreased exploration time with a novel object, and reduced entry time and time spent in the center. Increased glutamate concentration, decreased glutamate receptor NMDA subunits (NR1, NR2B) and transcription factors CREB1 and CaMKIV mRNAs and increased cFos and early growth response 1, reduced postsynaptic density protein-95, synaptophysin mRNAs, were observed in high-dose PSNP-treated rats. Moreover, antioxidative stress markers such as superoxide dismutase and catalase were significantly decreased whereas inflammatory cytokines (interleukin 1β, tumor necrosis factor-α) and microglial marker (ionized calcium-binding adapter molecule 1) were significantly higher in high-dose PSNP-treated rats. Our results indicate oral exposure to PSNPs induced anxiety-like behavior and learning, memory impairment by altering neuron-glia-immune cells interaction at synaptic regions in the rat hippocampus. This study would be helpful to understand the association between MP pollution and increasing neurological disorders like dementia, anxiety, and Alzheimer's disease in humans.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 9","pages":"507-521"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the toxicological findings from 90-day and 2-year repeated-dose toxicity studies of pesticides in rats. 大鼠90天和2年重复剂量农药毒性研究毒理学结果的特征。

IF 1.5 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.523

Yu Harakawa, Minami Shibata, Hiroaki Suzuki, Naofumi Takahashi, Atsushi Shiga, Kosuke Mizuno, Takuomi Hosaka, Ryota Shizu, Jun-Ichi Takeshita, Kouichi Yoshinari

{"title":"Characterization of the toxicological findings from 90-day and 2-year repeated-dose toxicity studies of pesticides in rats.","authors":"Yu Harakawa, Minami Shibata, Hiroaki Suzuki, Naofumi Takahashi, Atsushi Shiga, Kosuke Mizuno, Takuomi Hosaka, Ryota Shizu, Jun-Ichi Takeshita, Kouichi Yoshinari","doi":"10.2131/jts.50.523","DOIUrl":"https://doi.org/10.2131/jts.50.523","url":null,"abstract":"Repeated-dose toxicity (RDT) studies in rats are crucial for assessing the risks of chemicals, including pesticides, by elucidating their toxicological properties. In this study, we collected and comprehensively analyzed the results of rat RDT studies on pesticides. For over 350 pesticides across more than 400 studies, all toxicological findings observed in 90-day and 2-year RDT/carcinogenicity studies in rats were organized into 1,092 endpoints using our previously established three-level categorization system. The liver, kidney, thyroid gland, and spleen were identified as the primary non-tumor targets in both the 90-day and 2-year studies. In the 2-year study, the liver, thyroid gland, testis, and uterus were found to be the primary tumor targets, with significant sex differences in liver and thyroid tumors. We also identified findings observed at the lowest observed adverse effect level (LOAEL) for each study. Finally, analyses by pesticide class, based on their mode of action classification, demonstrated class effects on the toxicological findings observed and the LOAEL values. Taken together, these results provide valuable insights into toxicological endpoints that should be evaluated in further mechanistic studies and considered for the safety assessment of each class of pesticides.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 10","pages":"523-546"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of systemic inflammation by welding fume exposure in office workers as well as welders in welding factories. 办公室工作人员和焊接工厂的焊工接触焊接烟雾引起全身炎症。

IF 1.8 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.215

Mayumi Tsuji, Chihaya Koriyama, Tatsuto Nakane, Susumu Ueno, Yasuhiro Ishihara

{"title":"Induction of systemic inflammation by welding fume exposure in office workers as well as welders in welding factories.","authors":"Mayumi Tsuji, Chihaya Koriyama, Tatsuto Nakane, Susumu Ueno, Yasuhiro Ishihara","doi":"10.2131/jts.50.215","DOIUrl":"https://doi.org/10.2131/jts.50.215","url":null,"abstract":"Welding fumes are metal particles of 1 µm or less generated during welding. Welding fumes generated in welding factories spread throughout the workplace. However, the effects of exposure have been measured primarily in welding workers, and no research has been conducted on the effects of fumes on workplace office workers. In this study, we recruited welding and office workers who worked in the same factories at ten workplaces in Japan, mainly in the Kyushu and Kanto regions, and separated their serum after blood sampling. We also obtained serum from the general subjects of Minami-Kagoshima City, which is located far from the welding factory. Cytokines and chemokines were quantified in the serum samples, and the concentration of interleukin (IL)-1β was significantly increased in office workers and welders compared with general subjects. Importantly, the serum concentrations of IL-12p70, IL-17A, IL-33, tumor necrosis factor α, and C-C motif chemokine ligand 3 in office workers were significantly higher than those in the general subjects, and there was no significant difference in the levels of these inflammatory molecules between welders and general subjects. This study suggests that office workers exposed to high fume concentrations exhibit increased systemic inflammation. Exposure assessments should be conducted not only for welders but also for office workers to reduce exposure risks.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 5","pages":"215-221"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicological effects of Sb(III), Sb(V), and NMG-Sb(V) in human lung, kidney, and liver cells: cytotoxicity and fibrotic factor induction. Sb（III）、Sb(V)和NMG-Sb(V)在人肺、肾和肝细胞中的毒理学效应：细胞毒性和纤维化因子诱导。

IF 1.8 4区医学

Journal of Toxicological Sciences Pub Date : 2025-01-01 DOI: 10.2131/jts.50.283

Nicole Roldán, Marcelo Verdugo, Noriyuki Suzuki, Natali Zamora, Waldo Quiroz, Alexis Gonzalez, Javier Tognarelli, Yasumitsu Ogra

{"title":"Toxicological effects of Sb(III), Sb(V), and NMG-Sb(V) in human lung, kidney, and liver cells: cytotoxicity and fibrotic factor induction.","authors":"Nicole Roldán, Marcelo Verdugo, Noriyuki Suzuki, Natali Zamora, Waldo Quiroz, Alexis Gonzalez, Javier Tognarelli, Yasumitsu Ogra","doi":"10.2131/jts.50.283","DOIUrl":"https://doi.org/10.2131/jts.50.283","url":null,"abstract":"Antimony ecotoxicity studies are often hindered by the incorrect selection of Sb(III) standards and the application of concentrations that do not reflect real environmental exposure. In this study, we used environmentally relevant concentrations of inorganic Sb in its pentavalent [Sb(V)] and trivalent [Sb(III)] oxidation states, as well as the organic species NMG-Sb(V), which is present in Meglumine Antimoniate, to evaluate the effects of Sb on cell viability in human lung (A549), kidney (HEK293), and liver (HepG2) cell lines. Cell viability was assessed in these cells following treatment with 0.001 to 1 µg/L of Sb(V), 1 to 500 µg/L of Sb(III), and 0 to 1000 mg/L of MA. We also measured ROS production and the expression of the profibrotic markers CTGF, α-SMA, and PAI-1, which are associated with fibrosis activation. No significant changes in cell viability were observed in HepG2 and A549 cells. However, in HEK293 cells, viability decreased by 20-40% at Sb(III) concentrations between 1 µg/L and 1 mg/L. CTGF expression was significantly increased at 17 µg/L of Sb(III), while α-SMA and PAI-1 expression increased at 21 µg/L of Sb(V). These findings suggest that different species of Sb can induce increased expression of mRNA for fibrotic genes in human liver and kidney cell lines at concentrations found in the environment.","PeriodicalId":17654,"journal":{"name":"Journal of Toxicological Sciences","volume":"50 6","pages":"283-292"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0