European Journal of Life Sciences最新文献

Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species 测定三种地方性马鞭草的细胞毒性、抗氧化活性和 LC/MS-MS 图谱

European Journal of Life Sciences Pub Date : 2023-12-29 DOI: 10.55971/ejls.1364354

Sevim Küçük, M. Has, Fatih Göger, Filiz Özdemir, Z. Incesu

{"title":"Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species","authors":"Sevim Küçük, M. Has, Fatih Göger, Filiz Özdemir, Z. Incesu","doi":"10.55971/ejls.1364354","DOIUrl":"https://doi.org/10.55971/ejls.1364354","url":null,"abstract":"The Verbascum genus includes many species used in folk medicine or the treatment of various diseases. In this study, the cytotoxic, antioxidant activity, and LC/MS-MS profiles of three Verbascum species, which are endemic in Eskişehir and its surroundings, were investigated. The cytotoxic effects of methanol extract of V.detersile, V. eskisehirensis, and V.gypsicola species on the cervical (HeLa) and ovarian cancer (SKOV-3) cells were investigated using a colorimetric assay. The results indicated that cytotoxic effect was not observed after treatment of SKOV-3 cells with Verbascum. On the other hand, the cytotoxic activity of V. detersile was found to be 0.1910 mg/dL and 1.057 mg/dL for HeLa cells after 24 or 48 hours incubation with V.detersile, respectively. The antioxidant activity was determined as 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC assay), and also the total phenolic content of the samples was found. Total phenols were estimated as Gallic acid equivalents (GAE), expressed as mg Gallic acid/in 1g extract previously described by Singletton. LC/MS profiles separation and detection of phytochemicals of the extract were performed on a Shimadzu UPLC system consisting of a vacuum degasser, an autosampler (SIL20A Shimadzu Autosampler), a binary pump (LC20AD Shimadzu), an oven (CTO20A Shimadzu Column Oven) and DAD dedector (SPD M20A Shimadzu DAD Detector). In terms of antioxidant activity, V. gypsicola was found to have the least antioxidant activity among the three extracts, which is also correlated with the total amount of phenolic content in its content. In this way, it differs from other species. V. detersile exhibits a different chemical profile from the other two species with the iridoid catalpol derivatives it contains. Apigenin pentoside is the only flavonoid molecule detected in V. detersille.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"77 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139146514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPLC method for simultaneous quantification of lumacaftor and ivacaftor bulk and pharmaceutical formulations 高效液相色谱法同时定量分析 lumacaftor 和 ivacaftor 散剂及药物制剂

European Journal of Life Sciences Pub Date : 2023-12-29 DOI: 10.55971/ejls.1367996

Saniye Özcan, Abeer Elriş, Serkan Levent, N. O. Can

{"title":"HPLC method for simultaneous quantification of lumacaftor and ivacaftor bulk and pharmaceutical formulations","authors":"Saniye Özcan, Abeer Elriş, Serkan Levent, N. O. Can","doi":"10.55971/ejls.1367996","DOIUrl":"https://doi.org/10.55971/ejls.1367996","url":null,"abstract":"In 2015, the Food and Drug Administration granted approval for the use of lumacaftor 200 mg and ivacaftor 125 mg in the treatment of cystic fibrosis patients who possess the F508del mutation, namely those who are 12 years of age or older. Since its approval, the medicine has been implemented in clinical settings, although the presence of numerous disputes, with the aim of mitigating disease symptoms and enhancing the overall quality of life. Given the existing gaps in the literature regarding the analysis of the amalgamation of these two active substances, a straightforward and practical HPLC approach has been devised in adherence to the guidelines outlined in the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) Q2(R1) document. To accomplish this objective, the process of separation was successfully carried out using a monolithic silica stationary phase (Chromolith High Resolution RP-18e, 100 mm × 4.6 mm i.d., Merck KGaA, Darmstadt, Germany). The separation process was conducted using a gradient mode. The initial composition of the mobile phase consisted of acetonitrile and a phosphate buffer solution with a concentration of 0.030 M and a pH of 3.5. The flow rate was recorded as 1.0 mL/min, and avanafil was used as an internal standard. The improved and verified approach has demonstrated successful application in bulk and pharmaceutical formulation evaluations when utilizing the ivacaftor/lumacaftor combination.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"95 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139147291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile components of Ferulago aucheri Boiss. (Apiaceae) Ferulago aucheri Boiss.(Apiaceae）

European Journal of Life Sciences Pub Date : 2023-12-29 DOI: 10.55971/ejls.1366082

Esma Ocak, Filiz Eğin Kolata, Mine Kürkçüoğlu, Sevim Küçük

引用次数: 0

Evaluation of volatile components of Achillea millefolium L. essential oil Achillea millefolium L. 精油挥发性成分的评估

European Journal of Life Sciences Pub Date : 2023-12-29 DOI: 10.55971/ejls.1381369

Gözde Öztürk, D. Kırcı, Betül Demirci

{"title":"Evaluation of volatile components of Achillea millefolium L. essential oil","authors":"Gözde Öztürk, D. Kırcı, Betül Demirci","doi":"10.55971/ejls.1381369","DOIUrl":"https://doi.org/10.55971/ejls.1381369","url":null,"abstract":"The genus Achillea (Asteraceae) is distributed in Europe and the Middle East and has approximately 140 endemic species. There are 40 Achillea sp. in the Turkish flora and 20 of them are endemic. This genus has a widespread area all over the world. Also, it has many different uses in traditionally, such as diarrhea, abdominal pain, hemorrhoids and wound healing. Achillea sp. is also widely used as food. Achillea millefolium L. is known as “Yarrow” and has been used as a wound healer, diuretic, appetite stimulant and menstrual bleeding in Türkiye. The aerial parts of the plant are used in a variety of forms, including infusion, tincture, liquid extract, total extract, bath. It has also been stated that the essential oil of the plant is used in mouth sores and dental health due to its antimicrobial effect. It is also preferred in the treatment of aphtha and wounds in the mouth. Within the scope of this project, essential oil of A. millefolium was obtained from European Pharmacopoeia (9th Edition) quality, supplied from German commercial source, with the Clevenger apparatus for 3 h. The chemical composition of the essential oil obtained was elucidated. A total of 67 components were identified. Chamazulene (6.8%), caryophyllene oxide (5.8%), torilenol (5.6%), (E)-nerolidol (4.3%), borneol (4.0%) were found as major constituents of A. millefolium essential oil. The original value of this study, aim of this study is to conduct a pharmacognosic examination of the European Pharmacopoeia quality A. millefolium, one of the Achillea sp. that is of great importance worldwide due to medicinal proporties, especially herbal tea. With this study, the chemical composition of the volatile components of the A. millefolium was elucidated.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"58 s205","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139145900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmaceutical supply chain: The importance of outsourcing 医药供应链：外包的重要性

European Journal of Life Sciences Pub Date : 2023-12-29 DOI: 10.55971/ejls.1395960

Özlem Akbal Dağıstan

{"title":"Pharmaceutical supply chain: The importance of outsourcing","authors":"Özlem Akbal Dağıstan","doi":"10.55971/ejls.1395960","DOIUrl":"https://doi.org/10.55971/ejls.1395960","url":null,"abstract":"A vast web of organizations, companies, and teams involved in creating, researching, and producing drugs and associated products makes up the pharmaceutical sector. In this context, the term “supply chain” refers to the network of individuals, processes, information, and resources that transform raw materials and parts into finished goods and services before being supplied to customers. The pharmaceutical supply chain system, then, offers customers medications in the right amount, at the right time, with acceptable quality, and for the lowest feasible cost. Pharmaceutical companies are increasingly contracting out their supply chain activities due to the severe push to keep R&D expenses under control. Instead of being a cost-cutting measure, global supply chain outsourcing can be seen as a strategic competitive weapon that can improve positional advantage, offer production flexibility, and meet the ever-increasing expectations of final consumers. Global supply chain outsourcing is crucial for pharmaceutical firms to enhance performance and profit margins by leveraging core skills and resources beyond other strategies which can lower risk, increase flexibility, improve returns on capital, and improve a company’s ability to respond to the needs of its shareholders and consumers. Even while there is widespread recognition of the alluring advantages of global outsourcing, many of the related concerns are frequently disregarded. In addition to briefly discussing risk assessment methods, the goal of this work is to offer manufacturer-focused insight into supply chain-related outsourcing concerns within the pharmaceutical industry.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139148234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of thiazole derivatives as cholinesterase inhibitors with antioxidant activity 合成具有抗氧化活性的胆碱酯酶抑制剂噻唑衍生物

European Journal of Life Sciences Pub Date : 2023-12-29 DOI: 10.55971/ejls.1374823

Abdüllatif Karakaya, Zahra Maryam, Tuğba Erçetin, U. Acar Çevik

引用次数: 0

Evaluation of in vitro hepatotoxicity of perampanel in comparison with carbamazepine: old versus new 评价perampanel与卡马西平的体外肝毒性:新旧对比

European Journal of Life Sciences Pub Date : 2023-08-28 DOI: 10.55971/ejls.1324501

Gülnar Farmanlı, S. Ilgın, B. Ergun, Merve Baysal, A. Karaduman, Özlem Atlı Eklioğlu

引用次数: 0

Etiopathogenesis of depression and experimental depression models used in preclinical studies 抑郁症的发病机制及用于临床前研究的实验性抑郁症模型

European Journal of Life Sciences Pub Date : 2023-08-28 DOI: 10.55971/ejls.1327521

Ümmühan Kandemir

{"title":"Etiopathogenesis of depression and experimental depression models used in preclinical studies","authors":"Ümmühan Kandemir","doi":"10.55971/ejls.1327521","DOIUrl":"https://doi.org/10.55971/ejls.1327521","url":null,"abstract":"Depression is the most frequent psychiatric illness among mood disorders, affecting approximately 10% of adults. Especially recurrent and moderate/severe depression can become a serious public health problem by impairing people’s life quality. The monoamine hypothesis is the most widely accepted hypothesis for clarifying the pathophysiology of depression. Depression’s pathogenesis and etiology, however, are still poorly understood. Tricyclic antidepressants, monoamine oxidase inhibitors, selective serotonin or noradrenaline reuptake inhibitors, different atypical antidepressants, and electroconvulsive therapy are currently available therapies for depression. Although these treatment options are effective, a large number of patients do not respond to treatment or do not attain long-term remission. Furthermore, present antidepressants used in clinics have disadvantages such as delayed onset of effects, side effects, and patient compliance problems. Therefore, the discovery of new antidepressant medications is crucial. Animal models are critical in investigating the etiology of depression and developing novel treatments. Hence, in this review, the main mechanisms involved in the etiopathogenesis of depression and the experimental depression models used in preclinical studies have been demonstrated.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"160 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115747780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new HPLC method for selexipag analysis in pharmaceutical formulation and bulk form 一种新的高效液相色谱法分析药物制剂和原料药中的selexipag

European Journal of Life Sciences Pub Date : 2023-08-28 DOI: 10.55971/ejls.1320502

Saniye Özcan, Egemen Güvenç Öğüt, Serkan Levent, N. O. Can

{"title":"A new HPLC method for selexipag analysis in pharmaceutical formulation and bulk form","authors":"Saniye Özcan, Egemen Güvenç Öğüt, Serkan Levent, N. O. Can","doi":"10.55971/ejls.1320502","DOIUrl":"https://doi.org/10.55971/ejls.1320502","url":null,"abstract":"Selexipag is a new non-prostanoid prostacyclin receptor agonist used to treat pulmonary arterial hypertension. Selexipag is a long-acting IP receptor agonist with a shorter half-life than all other licensed drugs targeting the prostacyclin pathway, mostly administered intravenously or by subcutaneous infusion or inhalation. In this study, a new high performance liquid chromatography (HPLC) method was developed to analyze Selexipag in bulk and pharmaceutical formulations. The method used a column with Supelco Ascentis® Express (Sigma Aldrich, USA) model phenyl hexyl functional group (100×4.6 mm, ID, 2.7µm). Chromatographic separation was in isocratic elution mode, and the mobile phase mixture was acetonitrile containing 0.1% formic acid: water containing 0.1% formic acid (60:40, v/v) ratio. The method was linear in the concentration range of 15.7-117.6 µg/mL, and the LOD and LOQ were obtained as 2.4 and 3.1 µg/mL, respectively. Various method parameters have been tested according to the ICH Q2(R1) manual, and it is a method with high accuracy and precision. Therefore, the developed method is suitable for selexipag’s bulk and pharmaceutical formulation analysis.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129313905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral medicine administration errors in a patient with an enteral feeding tube 肠内喂食管患者的口服给药错误

European Journal of Life Sciences Pub Date : 2023-08-28 DOI: 10.55971/ejls.1316049

Özgenur Geridönmez, Kamer Tecen Yücel, Uygar Olgen

{"title":"Oral medicine administration errors in a patient with an enteral feeding tube","authors":"Özgenur Geridönmez, Kamer Tecen Yücel, Uygar Olgen","doi":"10.55971/ejls.1316049","DOIUrl":"https://doi.org/10.55971/ejls.1316049","url":null,"abstract":"Proper nutrition is vital for all patients with an expected lifespan. It is strongly recommended that patients whose oral intake is impaired or suspected to be impaired should be provided nutritional support. Concurrent medication administration during enteral nutrition may result in complications unless necessary precautions are taken. This study presents a case of a 94-year-old male patient with poor general health condition and being treated in a palliative care service. The patient was fed with an enteral feeding tube for seven drugs. There have been two tube occlusions causing the replacement of the tube since the enteral feeding tube was placed.The clinical pharmacist checked how the patient’s drugs were given through a nasogastric tube and how the patient’s relatives administered the drugs. Inappropriate dosage form selections and errors in administration through the nasogastric tube were identified. The interaction and incompatibility of the patient’s medications with the nutritional formula were also investigated. The clinical pharmacist informed the person giving the medicine to the patient about the correct administration of the medicine from the tube. The proper method for administering medications from the tube was ensured accordingly. Following the clinical pharmacist’s training, it was observed that the nasogastric tube was correctly used to administer the drug and the patient being monitored had no tube obstruction in the later phases of the treatment.It may be beneficial for a pharmacist to review drug dosage forms and applications in patients with a feeding tube in order to ensure correct administration and avoid undesired drug interactions.","PeriodicalId":176179,"journal":{"name":"European Journal of Life Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114137567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0