Jpc-journal of Planar Chromatography-modern Tlc最新文献_第5页

Insights into the evolution and future of high-performance thin-layer chromatography in routine quality control: a review 高性能薄层色谱法在常规质量控制中的发展与未来：综述

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-12-15 DOI: 10.1007/s00764-023-00265-w

Thi Kieu Tiên Do, Eike Reich

引用次数: 0

Effect of relative humidity on the thin-layer chromatographic separation of E 472 emulsifiers 相对湿度对 E 472 乳化剂薄层色谱分离的影响

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-12-11 DOI: 10.1007/s00764-023-00260-1

Katharina Schuster, Claudia Oellig

{"title":"Effect of relative humidity on the thin-layer chromatographic separation of E 472 emulsifiers","authors":"Katharina Schuster, Claudia Oellig","doi":"10.1007/s00764-023-00260-1","DOIUrl":"https://doi.org/10.1007/s00764-023-00260-1","url":null,"abstract":"E 472 emulsifiers are organic acid esters of mono- and diacylglycerides. These emulsifiers are categorized into six subgroups (E 472a-f) based on the specific acid component, e.g., acetic acid, lactic acid, citric acid, tartaric acid, or mixtures of tartaric and acetic acid. The present study investigated the effect of relative humidity (RH) on the thin-layer chromatographic separation of four E 472 emulsifier subgroups, addressing practical concerns due to RH variations between seasons and within laboratories. Three RH-setting techniques affecting plate activity and chamber climate were assessed using saturated salt and aqueous sulfuric acid solutions. Aqueous sulfuric acid solutions for RH adjustment resulted in decreased hRF values, while no trends in hRF value changes were observed for saturated salt solutions. Unlike RH itself, the RH setting technique led to substantial changes in the chromatographic fingerprint. Thus, the choice of the RH setting method should be tailored to the specific analyte and analysis goal. Despite variations in chromatographic fingerprints between the three RH setting techniques, the fingerprint remained consistent within the same technique across the tested RH range.","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":"114 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138567689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical analysis-based green planar chromatographic methodology for the quality assessment of food supplements: a case study on Origanum vulgare L. commercial products 基于统计分析的绿色平面色谱方法在食品补充剂质量评价中的应用——以牛头草商业产品为例

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-11-30 DOI: 10.1007/s00764-023-00261-0

Marko Jović, Petar Ristivojević, Vukosava Živković-Radovanović, Filip Andrić, Ivica Dimkić, Dušanka Milojković-Opsenica, Jelena Trifković

{"title":"Statistical analysis-based green planar chromatographic methodology for the quality assessment of food supplements: a case study on Origanum vulgare L. commercial products","authors":"Marko Jović, Petar Ristivojević, Vukosava Živković-Radovanović, Filip Andrić, Ivica Dimkić, Dušanka Milojković-Opsenica, Jelena Trifković","doi":"10.1007/s00764-023-00261-0","DOIUrl":"https://doi.org/10.1007/s00764-023-00261-0","url":null,"abstract":"Quality assessment is the most important step in the production of effective and safe food supplements. The aim of this study is to develop a green, simple, and high-throughput procedure for the quality assessment of Origanum vulgare L. commercial products using high-performance thin-layer chromatography (HPTLC)/bioautography with a multicriteria decision method, sum of ranking differences (SRD). Direct bioautography assays were developed for the identification of constituents with antibacterial activity tested against Gram-positive (Bacillus subtilis, Micrococcus lysodeikticus) and Gram-negative (Escherichia coli) strains, antioxidative (DPPH) activity, and enzyme (α-amylase) activity. Combination of the leave-one-out cross-validation methodology of SRD values and significant differences by post hoc Wilcoxon matched pairs test were used to evaluate commercial varieties. It was proved that suggested bioautography/chemometric screening methodology could be used as guidelines for the selection of any theoretical ideal product. Analysts may have benefits from this time-saving and streamlined quality profiling.","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":"237 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile chemical hydrophobization of thin-layer plates by vapor deposition of methyltrimethoxysilane for reversed-phase chromatography 反相色谱用甲基三甲氧基硅烷气相沉积薄层板的易化学疏水

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-11-30 DOI: 10.1007/s00764-023-00258-9

Julien Jaxel, Alice Merio, Nadine Kohlhuber, Marco Beaumont, Rupert Wimmer, Thomas Rosenau, Christian Hansmann, Falk Liebner, Stefan Böhmdorfer

{"title":"Facile chemical hydrophobization of thin-layer plates by vapor deposition of methyltrimethoxysilane for reversed-phase chromatography","authors":"Julien Jaxel, Alice Merio, Nadine Kohlhuber, Marco Beaumont, Rupert Wimmer, Thomas Rosenau, Christian Hansmann, Falk Liebner, Stefan Böhmdorfer","doi":"10.1007/s00764-023-00258-9","DOIUrl":"https://doi.org/10.1007/s00764-023-00258-9","url":null,"abstract":"Reversed-phase chromatography is based on a polar mobile phase and an apolar stationary phase. This separation mode is regularly used in planar (thin-layer) chromatography, and the necessary plates are commercially available. We investigated the preparation of hydrophobic plates for thin-layer chromatography by chemical vapor sorption of methyltrimethoxysilane directly onto a normal-phase plate. For this, a commercial normal-phase plate is simply exposed to the vapors of the reagent in a closed vessel. The obtained plates were characterized by infrared spectroscopy and dynamic water vapor sorption, which reported an extensive conversion of free hydroxyl groups by the reagent. The obtained plates were hydrophobic with a water contact angle close to 135°. The extent of hydrophobization precluded the use of pure water as an eluent while mixtures with organic solvents were perfectly adequate. The plates’ chromatographic performance was compared with that of C18 and paraffin-coated plates. For this, a set of parabens was separated with mixtures of acetone and water. The height of a theoretical plate was similar for the hydrophobized and the C18 plates (50–90 µm) and larger for the paraffin-coated ones. In contrast to the C18 plate, the hydrophobized and the paraffin-coated plates showed some selectivity for the analyte pair n-butylparaben and iso-butylparaben, which indicates a separation mechanism with the potential for regioselectivity.","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":"55 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a high-performance thin-layer chromatography method for the quantification of α-mangostin in three lesser-known Garcinia species of Assam 阿萨姆邦三种鲜为人知的藤黄属植物中α-山竹苷的高效薄层色谱定量方法的建立与验证

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-11-28 DOI: 10.1007/s00764-023-00259-8

Arpita Paul, Neelutpal Gogoi, Dipak Chetia, Md. Kamaruz Zaman

{"title":"Development and validation of a high-performance thin-layer chromatography method for the quantification of α-mangostin in three lesser-known Garcinia species of Assam","authors":"Arpita Paul, Neelutpal Gogoi, Dipak Chetia, Md. Kamaruz Zaman","doi":"10.1007/s00764-023-00259-8","DOIUrl":"https://doi.org/10.1007/s00764-023-00259-8","url":null,"abstract":"This study focusses on the development and validation of a simple, rapid and reliable high-performance thin-layer chromatography (HPTLC) method for the identification and quantification of α-mangostin in three underutilized Garcinia sp. The optimized mobile phase (chloroform‒methanol, 9:1, V/V) at 317 nm yielded sharp and well-defined peaks of α-mangostin with a constant RF value of 0.702, ensuring consistent identification and quantification. The regression equation showed a linear function with a correlation coefficient of 0.9997. The method was validated in terms of linearity, limits of detection and quantification (LOD and LOQ), precision, accuracy, repeatability and robustness, following the International Council for Harmonisation guidelines. The low %RSD values confirmed its excellent performance. Low values of LOD (24 ng per band) and LOQ (67.75 ng per band) indicate the sensitivity of the method. Significant quantities of α-mangostin were successfully quantified in all three Garcinia sp. using the developed HPTLC method. The study indicates the three Garcinia sp. of Assam as promising sources of α-mangostin. Overall, this research contributes to scientific knowledge as well as will help in the quality control of herbal drugs and formulations containing α-mangostin.","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":"50 11-12 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of white analytical chemistry-driven analytical quality risk assessment and design of experiments to multipurpose chromatographic method for the synchronous estimation of multiple drugs co-formulated with paracetamol 实施白色分析化学驱动的分析质量风险评估和实验设计，以多用途色谱法同步估计多种药物共配扑热息痛

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-11-22 DOI: 10.1007/s00764-023-00262-z

Pintu Prajapati, Minal Salunkhe, Veerashakar Pulusu, Shailesh Shah

{"title":"Implementation of white analytical chemistry-driven analytical quality risk assessment and design of experiments to multipurpose chromatographic method for the synchronous estimation of multiple drugs co-formulated with paracetamol","authors":"Pintu Prajapati, Minal Salunkhe, Veerashakar Pulusu, Shailesh Shah","doi":"10.1007/s00764-023-00262-z","DOIUrl":"https://doi.org/10.1007/s00764-023-00262-z","url":null,"abstract":"In the pharmaceutical industry, several fixed-dose paracetamol combinations have been manufactured and marketed. Several chromatographic methods have been documented in the literature for the quality control of these fixed-dose combinations (FDCs). Yet, these chromatographic procedures were developed using teratogenic and neurotoxic organic solvents that are harmful to the environment and dangerous to human and animal life. As a result, a multipurpose chromatographic technique was developed combining principles of white analytical chemistry, quality risk assessment, and experimental design. To reduce the time and cost of analysis, this chromatographic method needs a single chromatographic condition for the quality control of multiple FDCs of paracetamol. To safeguard the environment and human life, the suggested chromatographic process employs low-toxicity possible solvents. The analytical quality risk assessment methodology by Placket‒Burman design was used to identify significant analytical method risk parameters and analytical quality attributes. Response surface analysis by mixture design was used to optimize the identified critical method risk parameters. Using unique principles of white analytical chemistry, the proposed and published chromatographic techniques were assessed for their validation efficiency, greenness profile, time efficiency, and cost efficiency for the estimation of FDCs of paracetamol with multiple drugs.","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":"42 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Straightforward thin-layer chromatography–densitometric method for the determination of phyllanthin in Phyllanthus niruri from different phytogeographical zones 直接薄层色谱-密度法测定不同植物区带余甘子中余甘素的含量

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-11-20 DOI: 10.1007/s00764-023-00257-w

Kartini Kartini, Alfi Syahr Wijayati, Nikmatul Ikhrom Eka Jayani, Finna Setiawan, Ryanto Budiono

{"title":"Straightforward thin-layer chromatography–densitometric method for the determination of phyllanthin in Phyllanthus niruri from different phytogeographical zones","authors":"Kartini Kartini, Alfi Syahr Wijayati, Nikmatul Ikhrom Eka Jayani, Finna Setiawan, Ryanto Budiono","doi":"10.1007/s00764-023-00257-w","DOIUrl":"https://doi.org/10.1007/s00764-023-00257-w","url":null,"abstract":"Phyllanthus niruri contains various lignan compounds, whose concentrations vary depending on several factors. This study was intended to determine the phyllanthin content of P. niruri obtained from various locations in Indonesia by using thin-layer chromatography (TLC)‒densitometry to evaluate the effect of geographical factors on their quality. The TLC system comprised silica gel 60 F254 for the stationary phase, toluene‒ethyl acetate‒formic acid (15:10.5:1.5, V/V) for the mobile phase, and documentation under ultraviolet (UV) 254 nm light without chemical reagents. This developed method meets the specificity requirement, as marked by the identical UV spectrum between the phyllanthin sample and the standard (λmax = 279 and 230 nm). Further, it shows good linearity for phyllanthin concentrations in the range of 2.36‒11.8 µg/band (r = 0.9924), with LOD 0.532 µg/band and LOQ 1.612 µg/band. It also has good intraday and interday precision, as indicated by RSD of 8.87–9.43 and 6.94%, respectively. Eight of the 15 analyzed samples (collected from Batu, Blitar, Kediri, Nganjuk, Jember, Mojokerto, Banyuwangi, and Surabaya) contained only a trace amount of phyllanthin. In contrast, the other seven had varying levels of phyllanthin (1.376‒4.130 mg/g dried herbs). Using the Tawangmangu sample as the reference, these seven samples can be grouped into two: significantly lower phyllanthin contents (Tulungagung) and very significantly lower phyllanthin contents (Lumajang, Bangkalan, Pasuruan, Sidoarjo, and Gresik). It can be concluded that TLC‒densitometry designed in this research is a straightforward method that, at the same time, meets the validation parameters. Therefore, it can be repeated to analyze phyllanthin in P. niruri of different phytogeographical origins.","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":"4 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a simple thin-layer chromatography–smartphone method for plasma paracetamol quantification 一种简便的薄层色谱-智能手机定量血浆对乙酰氨基酚方法的建立与验证

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-08-29 DOI: 10.1007/s00764-023-00247-y

Rym Ferchiou, M. Soussi, Donia Ghedira, D. Ferchiou, W. Douki, M. Najjar

引用次数: 0

Quality by design approach for the quantitative determination of andrographolide, gallic acid and quercetin from polyherbal tablets by instrumental thin-layer chromatography 用薄层色谱法定量测定中药饮片中穿心莲内酯、没食子酸和槲皮素的质量设计方法

IF 1.6 4区化学

Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-08-27 DOI: 10.1007/s00764-023-00246-z

Monika Jadhav, P. Tatke

引用次数: 0

Thin-layer chromatographic enantioresolution of gatifloxacin using levocetirizine and levosalbutamol as chiral selectors 以左西替利嗪和左沙丁胺醇为手性选择剂的加替沙星薄层色谱对映分辨