Jurnal ProtobiontPub Date : 2018-11-27DOI: 10.26418/protobiont.v7i3.29853
Anggi Nurhadi Riza Linda Mukarlina
{"title":"Keanekaragaman Jenis Kantong Semar (Nepenthes spp.) di Kawasan Taman Wisata Alam Baning Kabupaten Sintang Kalimantan Barat","authors":"Anggi Nurhadi Riza Linda Mukarlina","doi":"10.26418/protobiont.v7i3.29853","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29853","url":null,"abstract":"Baning Natural Tourism Site located in Sintang district is the habitat of Nepenthes spp. The aim of the study is to determine the spesies and diversity of Nepenthes spp. in Baning Tourism site. The study was conducted in April - May 2017. Nepenthes spp. observation was done in 5 observation locations which was determined randomly based on the homogeneity of Nepenthes in Baning site with the total number of the observation area of 0.096 Ha. The results showed that there are 5 spesies of Nepenthes in Baning namely Nepenthes ampullaria, N. bicalcarata, N. mirabilis, N. gracilis, and N. x cantleyi. The diversity of Nepenthes spesies in Baning observation areas is to low, with the value of H' ranging from 1,075 to 0.616.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132265478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-11-27DOI: 10.26418/protobiont.v7i3.29855
Rizki Aprilliani Rafdinal Tri Rima Setyawati
{"title":"Komposisi Diatom (Bacillariophyceae) Perifitik pada Substrat Kaca di Sungai Kapuas Kecil Kota Pontianak Kalimantan Barat","authors":"Rizki Aprilliani Rafdinal Tri Rima Setyawati","doi":"10.26418/protobiont.v7i3.29855","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29855","url":null,"abstract":"Periphytic diatom (Bacillariophyceae) is diatom that lives attach on a substrate and it was dependent on the environmental conditions in an aquatic ecosystem. This study was aimed to determine the composition of periphytic diatom (Bacillariphyceae) on glass substrate and the condition of physical chemical water in Kapuas Kecil River. This study was conducted from September to December 2017. The sampling stations were chosen by purposive random sampling method and divided into four stations. At each station in the river were installed 5 baskets each with 5 glass slides. Samples were collected from three baskets with sampling intervals on day 2, 5, 10, 15, and 20. The result shows that the composition of periphytic diatoms (Bacillariophyceae) in Kapuas Kecil River consists of 29 genera from 2 orders (Pennales and Centrales) and 12 families. The most commonly genera found of periphytic diatom were Eunotia, Gomphonema, Frustulia and Nitzschia.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125346315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-10-11DOI: 10.26418/protobiont.v7i3.29088
Rizki Perdana Putri Diah Wulandari Rousdy, Ari Hepi Yanti
{"title":"Aktivitas Hepatoprotektif Ekstrak Metanol Buah Lakum (Cayratia trifolia (L.) Domin) Terhadap Diameter Vena Sentralis, Lebar Sinusoid dan Berat Hepar Tikus Putih (Rattus novergicus L.) yang Diinduksi Parasetamol","authors":"Rizki Perdana Putri Diah Wulandari Rousdy, Ari Hepi Yanti","doi":"10.26418/protobiont.v7i3.29088","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29088","url":null,"abstract":"Secondary metabolites of lakum (Cayratia trifolia (L.) Domin) fruit have been evaluated in previous researches. The fruit contains flavonoids, terpenoids and phenols which have antioxidant properties. The aim of this research was to determine the hepatoprotective activity of methanolic extract of lakum fruit in Wistar rats’ liver induced by high doses of paracetamol. Ripe lakum fruits were macerated with methanol as the solvent. Thirty-five male Wistar rats (Rattus novergicus L.) were used in this research and devided into 7 groups (normal, negative, positive, solvent, 115 mg kg-1, 230 mg kg-1, and 345 mg kg-1 of lakum fruit methanolic extract). Results showed that the effect of 230 mg kg-1 of lakum fruit methanolic extract (central vein diameter: 40,20 ± 6,97 µm; sinusoid wide: 5,60 ± 0,94 µm; and liver weight: 6,33 ± 0,98 g) were similar to the effect of silymarin (central vein diameter: 42,99 ± 6,60 µm; sinusoid wide: 4,49 ± 0,44 µm; and liver weight: 6,58 ± 0,88 g) as the standard drug.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122266205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-10-11DOI: 10.26418/protobiont.v7i3.29081
Nur Aprilyani Mukarlina Rizalinda
{"title":"Pertumbuhan Stek Batang Sirih Merah (Piper crocatum Ruiz.) Setelah Perendaman Dengan Ekstrak Bawang Merah (Allium cepa L.) Dan Air Kelapa (Cocos nucifera L.)","authors":"Nur Aprilyani Mukarlina Rizalinda","doi":"10.26418/protobiont.v7i3.29081","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29081","url":null,"abstract":"The Red betel (Piper crocatum Ruiz.) is one of the types of plants that can be used as a medicinal plant. The Red betel plant can be propagated through cuttings, but the ability of the Red betel leaves to stem cuttings rooted very low. The purpose of this research is to know the influence of the concentration of soaking onion extract and coconut water towards the growth of the stem cuttings of red betel. This study used a randomized complete design (RAL) factorial with two levels, namely the first factor extract of onion (B) that consists of 4 concentrations, namely control (0%), B1 (2,5%), B2 (4%), and B3 (5.5%). The second factor is cocnut water (K) consisting 0f 4 concentrations of control (0%), K1 (15%), K2 (20%) and K3 (25%), retrieved 16 combination treatments 3 replications to obtain 48 units of the experiment. The results showed that the combination of onion extract 5.5% + 25% coconut water shows most good for the number of leaves, plant height, wet weight of heading, the heading dry weight, root weight of wet and dry weight of roots.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116315240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-10-11DOI: 10.26418/protobiont.v7i3.29092
Tri Oktarini Adiaty Riza Linda Mukarlina
{"title":"Jamur Mikoriza Vesikular Arbuskular (MVA) Pada Tiga Jenis Tanah Rhizosfer Tumbuhan Lakum (Cayratia trifolia (L.) Domin)","authors":"Tri Oktarini Adiaty Riza Linda Mukarlina","doi":"10.26418/protobiont.v7i3.29092","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29092","url":null,"abstract":"The lakum plant (Cayratia trifolia (L.) Domin) is a wild plant so it is easily found in various types of soil, such as alluvial soil, ultisol soil and soil with salinity (saline soil). The ability of the lakum plant (C. trifolia) to grow on alluvial soil, ultisol soil, and saline soils is presumably cause by a role of Vesicular Arbuscular Mycorrhizal (VAM) fungi. This research aims to find out about VAM fungi in the rhizosphere of the lakum plant (C. trifolia) and the rate of VAM fungal infection in three types of rhizosphere soil of the lakum plant. This research was conducted for three months, starting from January to May 2018 at the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences of Tanjungpura University. The research procedure included several stages, namely sampling, isolation of spores by a combination of wet filter pouring technique, identification and characterization, and coloring and making root preparations with coloring method. Based on the results of the research, the spores of the VAM fungi found in alluvial soil consisted of the genera Acaulospora, Archaeospora, Glomus and Paraglomus. The spores of the genus of VAM fungi found on the saline soil were only the genus Glomus. The spores of the genus of VAM fungi found on ultisol soil consisted of the genera Acaulospora, Glomus and Paraglomus. The results of observations show that VAM fungal infection in alluvial soil was 25% (low), in saline soil 50.6% (high) and ultisol soil 53% (high)","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128340652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aktivitas Biologis Asap Cair Batang Manggis (Garcinia mangostana L.) Terhadap Viabilitas Streptococcussp. (L.10.3)","authors":"Lilis Susanti Elvi Rusmiyanto Rikhsan Kurniatuhadi","doi":"10.26418/protobiont.v7i3.29062","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29062","url":null,"abstract":"The wood vinegar of the stem of mangosteen (G. mangostana L.) is the result of pyrolysis of the stem which is condensed into steam. Wood vinegar contains acid and phenol compounds that have antibacterial activities. This research aims to determine the biological activities of the wood vinegar of the stem of mangosteen (G. mangostana L.) on the viability of Streptococcus sp. (L.10.3) and determine the concentration of wood vinegar of the stem of mangosteen (G. mangostana L.) which is effective in inhibiting the growth of Streptococcus sp. (L.10.3). This research used a completely randomized design (CRD) with 6 treatments consisting of wood vinegar with a concentration of 0.1 (Tl); 0.5 (T2); 1 (T3) and 1.5% (T4), negative controls namely sterile distilled water (T5), and positive control namely chlorhexidine of 0.2% (T6). The viability test used the dilution method and calculation of the number of colonies using the total plate count (TPC) method, each treatment was repeated four times. The results showed the increasing concentration of wood vinegar of the stem of mangosteen (G. mangostana L.) and the decreasing amount of Streptococcus sp. (L.IO.3). Wood vinegar at concentration 1.5% showed bacterial growth of 6.9 x 105 CFU / ml which was very different from sterile distilled water by 2.5 x 107 CFU / ml with inhibition up to 96.9% and not significantly different from chlorhexidine of 0.2%.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129695883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Daya Hambat Ekstrak Etanol Batang Lakum (Cayratia trifolia (L.) Domin) Terhadap Pertumbuhan Isolat Phytophthora sp. Im5 secara In Vitro","authors":"Fitrie Dwi Lestari Elvi Rusmiyanto PW Rikhsan Kurniatuhadi","doi":"10.26418/protobiont.v7i3.29064","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29064","url":null,"abstract":"Stem rot disease is caused by one of Phytophthora pathogenic fungi that attacks several types of cultivated plants. The alternative control against Phytophthora attack can be done with the use of natural fungicides. One of the herbs that can be untilized is the ethanol extract of lakum stem (Cayratia trifolia (L.) Domin). The aim of this research is to know the effect of ethanol extract of lakum stem and its secondary metabolite in inhibiting the growth of isolate Phytophthora sp. Im5. The research was conducted for three months starting from October to December 2017. This research used solid dilution method and completely randomized design (CRD) wth 8 levels of treatments, i.e. positive control, negative control, ethanol extract concentration of 5, 10, 15, 20, 25 and 30 mg/ml. The test for secondary metabolite compounds was done by phytochemical screening method. The result showed that the concentration of 30 mg/ml has the biggest antifugal activity with the inhibition percentage of 34.98%. The activity of lakum stem ethanol extract against isolate Phytophthora sp. Im5 was included in moderate catagory. The results of phytochemical screening showed that the ethanol extract of lakum stem contained alkaloid, flavonoid and tannin compounds.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"43 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130695330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-10-11DOI: 10.26418/protobiont.v7i3.29090
Noni Yunizar Rahmawati Kustiati
{"title":"Patogenitas Isolat Jamur Entomopatogenik Metarhizium anisopliae terhadap Lalat Rumah Musca domestica L. (Diptera: Muscidae)","authors":"Noni Yunizar Rahmawati Kustiati","doi":"10.26418/protobiont.v7i3.29090","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29090","url":null,"abstract":"Metarhizium anisopliae is one of the entomopathogenic fungi that can be used to control house fly, Musca domestica. The aim of this study is to determine the effectiveness of fungus Metarhizium anisopliae in killing house flies. This study used a Completely Randomized Design (CRD) with 5 treatment concentration of Metarhizium anisopliae fungal suspension concentration of 1x106 conidia/mL, 3x106 conidia/mL, 5x106 conidia/mL, 7x106 conidia/mL, 9x106 conidia/mL with each repetition three times. The fungal pathogenicity was determined by the lethal time of 50% flies (LT50) for each concentration using Probit analysis. The result of this study showed that the time required to killing 50% of flies in consecutive consentrations was 7 days with concentrations ranging from 3x106 conidia/mL until 5x106 conidia/mL. In conclusion, the fungus Metarhizium anisopliae is effectively deadly pest house flies Musca domestica.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"330 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115875241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-10-11DOI: 10.26418/protobiont.v7i3.29077
Firgian Dine Wulandara Rafdinal Riza Linda
{"title":"Etnobotani Tumbuhan Obat Suku Melayu Desa Durian Sebatang Kecamatan Seponti Kabupaten Kayong Utara","authors":"Firgian Dine Wulandara Rafdinal Riza Linda","doi":"10.26418/protobiont.v7i3.29077","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29077","url":null,"abstract":"This research was conducted with the aim to know the types of medicinal plants, parts of plants used as medicines and methods of processing and the use of plants as a traditional medicine. The research was conducted four months, starting from September to December 2017. The research was conducted in Durian Sebatang Village, Seponti Sub-District, North Kayong District. The research method using Purposive Sampling, with selected respondents as many as 8 people, belong to two categories namely 2 people shaman giving birth and 6 people herbalist. The design of this research is observation, then conducted semi-structured interviews and filling questionnaires by respondents. The results showed that there are 43 families and 93 species of plants that have potential as drugs. The highest percentage of families is Zingiberaceae (9,7%) and Euphorbiaceae (9,7%). Part of a widely used plant is the leaves (48%), while the way of processing and the use of medicinal plants is in boiled (48%) and drunk (53%). Taking medicinal plants is mostly done in the forest (36%).","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124032479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurnal ProtobiontPub Date : 2018-10-11DOI: 10.26418/protobiont.v7i3.29063
Resti Elvi Rusmiyanto PW Diah Wulandari Rousdy
{"title":"Efek Paparan Musik Klasik, Hard Rock dan Murottal Terhadap Pertumbuhan Vegetatif Tanaman Bayam Merah (Alternanthera amoena Voss)","authors":"Resti Elvi Rusmiyanto PW Diah Wulandari Rousdy","doi":"10.26418/protobiont.v7i3.29063","DOIUrl":"https://doi.org/10.26418/protobiont.v7i3.29063","url":null,"abstract":"This research was conducted to determine the effect of classical music, hard rock and murottal against to vegetative growing of red spinach plants. The research used a completely randomizes design with 4 treatments and 5 replicates. ANOVA result showed that music exposure had significant effect on plant growth. Murottal exposure gave optimal result on plant height 35,70 cm, leaf area 43,40 cm2, root length 9,40 cm, stomata porous length 23,00 µm, wet weigh 15,59 g, and dry weight of the plant 11,25 g. Exposure to hard rock music gives optimal results on the amount of leaf chlorophyll is worth 34,52 spad unit.","PeriodicalId":175944,"journal":{"name":"Jurnal Protobiont","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116504445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}