Journal of ultrastructure research最新文献_第4页

Substructure of a cytoskeletal complex associated with the hamster sperm acrosome 与仓鼠精子顶体相关的细胞骨架复合体的亚结构

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90044-8

Gary E. Olson, Virginia P. Winfrey

引用次数: 32

Acrosome differentiation inMolgula manhattensis (Ascidiacea, Tunicata) molgula manhattensis(海鞘科，囊科)顶体分化

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90043-6

M. Fukumoto

引用次数: 16

Transtubular transport of proteins in rabbit proximal tubules 兔近端小管中蛋白质的管突转运

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90041-2

Jørn Theil Nielsen, Søren Nielsen, Erik Ilsø Christensen

{"title":"Transtubular transport of proteins in rabbit proximal tubules","authors":"Jørn Theil Nielsen, Søren Nielsen, Erik Ilsø Christensen","doi":"10.1016/0889-1605(85)90041-2","DOIUrl":"10.1016/0889-1605(85)90041-2","url":null,"abstract":"<div><p>The purpose of the present experiments was to study possible different pathways of intracellular transport of proteins after luminal and basolateral uptake in isolated rabbit proximal tubules. Tubules were exposed to cationized ferritin (CF) in the perfusion fluid and horseradish peroxidase (HRP) in the bath simultaneously or to HRP in the bath alone for 30 min. The peritubular fluid (bath) and perfusion fluid were then exchanged and the tubules either fixed immediately or allowed to function during chase-periods for 10, 20, 30, or 60 min before fixation to follow the migration of the proteins through the cells. The proteins were to a large extent found separated in different vacuoles and lysosomes at all time periods studied, indicating separate pathways after uptake via the luminal and basolateral membranes respectively. About 0.5% of the CF taken up by the cells was transported through the cells and became located in the intercellular spaces. HRP was transported from the peritubular fluid to the apical cytoplasm of the tubule indicated by a gradual accumulation of small HRP-containing vesicles, first in the basal part of the cells and then in the apical cytoplasm. In tubules perfused with both CF and HRP in the perfusate, the CF and HRP were found together in apical vacuoles and lysosomes. After perfusions with HRP alone, this tracer was found in similar large vacuoles and lysosomes in the apical cytoplasm, in contrast to the small HRP-filled vacuoles seen after uptake from the bath.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"92 3","pages":"Pages 133-145"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90041-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14947267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Investigation of the 50 S ribosomal subunit by electron microscopy and image analysis 50s核糖体亚基的电镜和图像分析研究

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90045-X

A. Verschoor , J. Frank , M. Boublik

引用次数: 33

Comparison between thylakoid composition and chloroplast ultrastructure in developing plants ofBrassica, Helianthus, Sisymbrium, andTanacetum 芸苔属、向日葵属、西茜草属和鹤尾草属发育植物类囊体组成和叶绿体超微结构的比较

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90046-1

A. Nurmi

引用次数: 7

Morphogenesis of the elastic fiber: An immunoelectronmicroscopy investigation 弹性纤维的形态发生:免疫电镜观察

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90047-3

A. Jaques, A. Serafini-Fracassini

{"title":"Morphogenesis of the elastic fiber: An immunoelectronmicroscopy investigation","authors":"A. Jaques, A. Serafini-Fracassini","doi":"10.1016/0889-1605(85)90047-3","DOIUrl":"10.1016/0889-1605(85)90047-3","url":null,"abstract":"<div><p>In this study a rabbit antiserum against human aortic elastin, which showed a high degree of species specificity in ELISA tests, was used to examine elastin fiber formation in the human fetal aorta between the ages of 14 and 23 weeks. Elastin was first detected by the antibody in the matrix of the 14-week-old specimen in association with the microfibrillar component. At this stage of development, the sections did not reveal structures morphologically identifiable as elastin. By the 17th week, discrete loci of elastin deposition were observed together with well-defined elastin fibrils. Only by the 23rd week did the aorta show the characteristic layering of elastic fibrils separating the myoblasts of the tunica media. In the latter specimen, the newly synthesized uncrosslinked elastin appeared to be unevenly distributed on the surface of elastic fibrils where it formed continuous strips of variable width arranged mostly in the form of spirals. This observation is discussed with respect to the proposals that (a) the morphogenesis of elastic tissue is a dynamic process involving a close interrelationship between elastic fibrils and elastogenic cells and (b) the morphogenetic movement of elastogenic cells plays an important role not only in the growth of elastic fibrils but also in the ultrastructural organization of the tissue.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"92 3","pages":"Pages 201-210"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90047-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14948003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Comparison between thylakoid composition and chloroplast ultrastructure in developing plants ofBrassica, Helianthus, Sisymbrium, andTanacetum 巴西草、向日葵、水芹和田菁发育中植物类囊体组成和叶绿体超微结构的比较

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90046-1

A. Nurmi

{"title":"Comparison between thylakoid composition and chloroplast ultrastructure in developing plants ofBrassica, Helianthus, Sisymbrium, andTanacetum","authors":"A. Nurmi","doi":"10.1016/0889-1605(85)90046-1","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90046-1","url":null,"abstract":"<div><p>The relationship between the chloroplast ultrastructure and the thylakoid membrane composition was observed during the growth of<em>Brassica rapa</em> ssp.<em>oleifera, Helianthus annuus, Sisymbrium altissimum</em>, and<em>Tanacetum vulgare</em>. The ultrastructural measurements were made on sections cut perpendicular to the grana. In all the species the young leaves had a low proportion of P<sub>700</sub> chlorophyll a-protein complex (CPI in photosystem I), whereas the proportion of chlorophyll a-protein complex of photosystem II (CPa) was fairly high. The amount of chlorophyll a-protein complex of photosystem II (CPa) decreased with age, whereas the amount of P<sub>700</sub> chlorophyll a-protein (CPI) complexes increased. The proportion of the light-harvesting chlorophyll a/b-protein (LHCP) complexes first increased and the declined as the plants aged. The chloroplast area in longitudinal sections was highest when the total content of light-harvesting chlorophyll a/b-protein (LHCP) was high and the values declined in older plants. During ageing, the ultrastructure of the chloroplast showed increase in the size of the grana and distinct profiles of stroma thylakoids extending between large grana. The granal stacks were largest, when the proportion of P<sub>700</sub> chlorophyll a-protein (CPI) was high and the total amount of light-harvesting chlorophyll a/b-protein (LHCP) low. When light is not limiting, the stage of development of the plant seems to have a significant effect on the thylakoid components and thylakoid ultrastructure.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"92 3","pages":"Pages 190-200"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90046-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72213475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Acrosome differentiation inMolgula manhattensis (Ascidiacea, Tunicata) 人疣状体（Ascidicacea，Tunicata）的顶体分化

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90043-6

Makoto Fukumoto

引用次数: 16

Author index for volume 92 第92卷作者索引

Journal of ultrastructure research Pub Date : 1985-09-01 DOI: 10.1016/0889-1605(85)90048-5

引用次数: 0

Ultrastructure of distal nephron cells in rat renal cortex 大鼠肾皮质远端肾元细胞的超微结构

Journal of ultrastructure research Pub Date : 1985-07-01 DOI: 10.1016/0889-1605(85)90132-6

Jens Dørup

引用次数: 41