Journal of Veterinary Science and Research最新文献_第2页

Genotyping of Toxoplasma gondii in sheep and cattle meat using PCR-RFLP technique 用PCR-RFLP技术分型羊和牛的刚地弓形虫

Journal of Veterinary Science and Research Pub Date : 2021-02-07 DOI: 10.30564/VSR.V2I2.2673

M. Rahdar, L. Arab, A. S. Zadeh

{"title":"Genotyping of Toxoplasma gondii in sheep and cattle meat using PCR-RFLP technique","authors":"M. Rahdar, L. Arab, A. S. Zadeh","doi":"10.30564/VSR.V2I2.2673","DOIUrl":"https://doi.org/10.30564/VSR.V2I2.2673","url":null,"abstract":"Toxoplasma gondii is an obligate, intracellular parasite, with worldwide distribution. The main source of infection for humans is livestock and meat-producer animals. The relationships between Toxoplasma genotype and biological characteristics of the parasite have already been identified. According to the pathogenicity of the parasite in laboratory animals, Toxoplasma is divided into three genotypes included type I, II and III. Understanding the genotype of the parasite, could help us to predict clinical features and severity of disease. The aim of this study was to identify genotypes of T. gondii in cattle and sheep meat and meat products in Ahvaz city southwest of Iran.One hundred and ninety samples of tongue, heart and muscles of sheep and cattle and meat products, including sausages and burgers, were collected from slaughterhouses and stores. To identify Toxoplasma gondii, DNA were extracted from samples and B1 gene were amplified by specific primers. To determine the genotype of T.gondii, PCR-RFLP was done on positive samples using by amplifying GRA6 gene and endonuclease Msel enzyme. Data analysis showed that the strain of the parasite in all positive samples belonged to genotype I.In this study the predominant Toxoplasma genotype was type I which can cause severe clinical symptoms in immunocompromised patients. Further research is needed to determine the genotype of the parasite in humans and other animals.","PeriodicalId":17588,"journal":{"name":"Journal of Veterinary Science and Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89372325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Alterations in Quality Parameters of Mastitic Milk 乳乳质量参数的变化

Journal of Veterinary Science and Research Pub Date : 2021-02-07 DOI: 10.30564/VSR.V2I2.2638

M. Azam, M. Naseer, Kiran Mumtaz, I. Muzammil, Khazeena Atta, R. Ahmed, A. I. Aqib

{"title":"Alterations in Quality Parameters of Mastitic Milk","authors":"M. Azam, M. Naseer, Kiran Mumtaz, I. Muzammil, Khazeena Atta, R. Ahmed, A. I. Aqib","doi":"10.30564/VSR.V2I2.2638","DOIUrl":"https://doi.org/10.30564/VSR.V2I2.2638","url":null,"abstract":"Quality milk production in modern dairy systems is facing many challenges. Salient in them is mastitis which is responsible for decline in milk production, altered milk composition and compromised udder health. The malaise consists of multiple bacterial etiologies which can be broadly classified into contagious pathogens and environmental pathogens S. aureus is being isolated invariably in all epidemiological studies, followed by E. coli. Pathogenic virulence in mastitis is often accounted due to microbial ability of producing wide array of virulence factors that enhances pathogenicity and sustainment potential in the epithelial linings of udder. Mastitis affects quality parameters of milk i.e. constitutional as well as mineral profile due to local damage and inflammatory mediators. It decreases the lactose secretion because of oxidative stress generated due to the formation of free radicals in the milk. In mastitic milk, IgG2 becomes the predominant antibody which is thought to be the main opsonin supporting neutrophil phagocytosis in the bovine mammary gland. Therefore, it plays a significant role in the battle against mastitis pathogens. Mastitis infected cow shows a notable elevated level of the sodium and chlorine and demoted level of calcium, potassium and inorganic phosphorus. In micro minerals, mastitis affects are pretty much same as in most macro minerals i.e. lower down their concentration in milk secretion. Consistent preventive strategy alongside strict surveillance and biosecurity is recommended for combating this challenge. ","PeriodicalId":17588,"journal":{"name":"Journal of Veterinary Science and Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81132634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drinking a structured water product on markers of hydration, airway health and heart rate variability in Thoroughbred racehorses: a small-scale, clinical field trial 纯种马饮用结构水产品对水合作用、气道健康和心率变异性的影响:一项小规模的临床现场试验

Journal of Veterinary Science and Research Pub Date : 2020-11-30 DOI: 10.30564/vsr.v2i2.2380

M. Lindinger

{"title":"Drinking a structured water product on markers of hydration, airway health and heart rate variability in Thoroughbred racehorses: a small-scale, clinical field trial","authors":"M. Lindinger","doi":"10.30564/vsr.v2i2.2380","DOIUrl":"https://doi.org/10.30564/vsr.v2i2.2380","url":null,"abstract":"Racehorses in training are in situations of repeated stress that may have effects on hydration and health, including airway health. The main hypothesis of this descriptive study was that daily consumption of a structured water (SW) product for 4 weeks will result in improved hydration, reduced markers of upper airway health concerns and increased heart rate variability. Two groups of Thoroughbred racehorses matched for physiological, training and racing attributes were studied for 4 weeks. One group (n = 17) received 10 L (~15%) of their daily water as SW (followed by ad libitum filtered deep well water) and the control group (n = 15) only filtered deep well water. Duplicate (two separate days) blood samples and bioelectrical impedance analysis (BIA) measures were obtained at baseline, 2 and 4 weeks. Hydration was assessed using BIA. The upper airway was assessed by nasopharyngeal endoscopy at baseline within 60 minutes of breezing (weekly near-race gallop pace). On weekly breeze days heart rate was recorded at rest, during exercise and recovery and data were analysed for heart rate variability. Compared to controls, horses drinking SW showed: (a) increased hydration by 2 weeks that was sustained to 4 weeks; (b) upper airway health (less mucous and less trace bleeding) post-breezing; and (c) increased heart rate variability (more restorative autonomic response) at rest. There were no performance benefits, no adverse events occurred, and blood hematological and biochemistry parameters were normal throughout. It is concluded that drinking 10 L daily of SW increased hydration and may have conferred some wellness benefits.","PeriodicalId":17588,"journal":{"name":"Journal of Veterinary Science and Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77164662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A comparative investigation on growth of three food born pathogenic bacteria inoculated with Withania somnifera: an invitro experimental study 三种食源性致病菌接种苦参菌的体外生长比较研究

Journal of Veterinary Science and Research Pub Date : 2020-10-14 DOI: 10.30564/vsr.v2i1.2052

Abdoljamal Azar, S. Salari, S. Sargolzaei

引用次数: 0

High Fat Diet Load Study in a Natural Obesity-Resistant Animal Model, Suncus murinus 自然抗肥胖动物模型鼠尾鼠的高脂肪饮食负荷研究

Journal of Veterinary Science and Research Pub Date : 2020-06-12 DOI: 10.36811/jvsr.2020.110011

Ming-Shou Zhang, Yi-Dan Dai, Hiraku Sasaki, Ke Ren, Zhi-Dan Wang, Jue-Fei Chen, Yasuhiko Yamamoto, Shuang-Qin Yi

{"title":"High Fat Diet Load Study in a Natural Obesity-Resistant Animal Model, Suncus murinus","authors":"Ming-Shou Zhang, Yi-Dan Dai, Hiraku Sasaki, Ke Ren, Zhi-Dan Wang, Jue-Fei Chen, Yasuhiko Yamamoto, Shuang-Qin Yi","doi":"10.36811/jvsr.2020.110011","DOIUrl":"https://doi.org/10.36811/jvsr.2020.110011","url":null,"abstract":"Our previous study, demonstrated the obesity-resistant phenomenon in the House musk shrew, Suncus murinus (S. murinus). In order to go further to explore the mechanism of the phenomenon of natural obesity resistance in S. murinus, we focused on the effects of diet on fat accumulation and metabolism. In this study, four-week-old male S. murinus were assigned to 1 of 2 experimental groups (n = 6 per group) and fed either a high-fat diet (HFD) or a normal trout diet (NTD) for 24 weeks. Body weight, food ingestion, visceral fat distribution, blood biochemistry and fecal lipids were monitored and analyzed in the HFD and NTD groups. It was found no differences in the average final body weight or body fat change between the two groups. Although animals fed HFD had similar serum triglyceride concentration to animals fed NTD, they had significantly higher phospholipid and total cholesterol concentrations than the NTD group. Furthermore, fecal lipid levels in the HFD group was significantly higher than those in the NTD group, suggesting that diet composition or energy density of the diet affects blood biochemistry. Although NTD-fed S. murinus had higher food ingestion than HFD-fed S. murinus, caloric intake remained almost the same. We speculated S. murinus may suppress obesity and control fat accumulation by controlling calorie intake. These results suggest that the obesity characteristics of S. murinus are not primarily due to dietary factors but to other mechanisms of regulation.\u0000\u0000Keywords: High fat diet; Mesenteric fat; Obesity-resistant; Suncus murinus","PeriodicalId":17588,"journal":{"name":"Journal of Veterinary Science and Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80884869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Multiple RT-PCR Detection of H5, H7, and H9 Subtype Avian Influenza Viruses and Newcastle Disease Virus H5、H7、H9亚型禽流感病毒和新城疫病毒的多重RT-PCR检测

Journal of Veterinary Science and Research Pub Date : 2019-12-01 DOI: 10.30564/vsr.v1i2.1429

F. Fei

引用次数: 0

Sero-prevalence of serological of Lawsonia intracellularis in domestic pigs in South Korea 韩国家猪胞内裂裂菌血清学调查

Journal of Veterinary Science and Research Pub Date : 2019-11-25 DOI: 10.36811/jvsr.2019.1100006

Jung-Ah Kim, Eun-Ok Kim, Van Giap Nguyen, Yong-Ho Park, Hee-Chun Chung, Bong- Kyun Park

引用次数: 0

Baseline survey of Ixodid Ticks Infesting Cattle in Northern State, Sudan 苏丹北部州牛只染蜱基线调查

Journal of Veterinary Science and Research Pub Date : 2019-10-05 DOI: 10.36811/jvsr.2019.110005

Mohamed Toum Elhaj, K. Taha, A. Ghali, S. Hassan, D. Salih, J. Ahmed, P. Clausen, A. M. E. Hussein

{"title":"Baseline survey of Ixodid Ticks Infesting Cattle in Northern State, Sudan","authors":"Mohamed Toum Elhaj, K. Taha, A. Ghali, S. Hassan, D. Salih, J. Ahmed, P. Clausen, A. M. E. Hussein","doi":"10.36811/jvsr.2019.110005","DOIUrl":"https://doi.org/10.36811/jvsr.2019.110005","url":null,"abstract":"Background and objective: Ixodid ticks are serious obstacle to development and utilization of animal resource in tropical countries like Sudan. Over 70 species of tick are prevalent in the Sudan in diverse ecological zones. The aim of this study was to provide a baseline data on tick species infesting cattle in the Northern State, Sudan as a prerequisite to design tick control policy in the state as well as the whole country.\u0000\u0000Materials and Methods: This study was conducted in April 2017 and included seven provinces of the state. Total body tick collection (n=1,098) was carried out from 365 heads of cattle. Tick identification to the genus and species level was carried out as well as statistical analysis using two-way ANOVA.\u0000\u0000Results: Nine tick species of two genera, Hyalomma and Rhipicephalus, were identified. The genus Hyalomma represented 82% of the collected ticks. Hyalomma rufipes was the most common tick species (48.5%) followed by Rhipicephalus praetextatus (14.9%), H. anatolicum (14.4%), H. excavatum (12.8%), H. dromedarii and R. sanguineus (3.4% each), H. impeltatum (2.2%), H. marginatum (0.46%) and the least prevalent tick species was R. evertsi evertsi (0.09%). The prevalence of tick infestation significantly varied within the age groups for H. anatolicum, H. rufipes and H. excavatum. The mean tick infestation was found to be independent on breed and sex of the animals.\u0000\u0000Conclusion: It is concluded that there were nine ticks species distributed in the State. Among these species is H. anatolicum, which is the vector of two vital diseases, bovine tropical theileriosis and malignant ovine theileriosis. Thus, tick control strategy, and creation of awareness among animal breeders on the problem associated with tick infestation are recommended.","PeriodicalId":17588,"journal":{"name":"Journal of Veterinary Science and Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77573159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Integrated Nutraceutical – Nutritional Approaches to Address Equine Leaky Gut Syndrome 综合营养-营养方法解决马漏肠综合征

Journal of Veterinary Science and Research Pub Date : 2019-09-30 DOI: 10.30564/vsr.v1i1.1057

M. Lindinger

引用次数: 1

Molecular Analysis of Pathogenicity Differences of Avian Paramyxovirus 1 Genotypes VI and VII in Chickens 禽副粘病毒1基因型VI和VII对鸡致病性差异的分子分析

Journal of Veterinary Science and Research Pub Date : 2019-09-30 DOI: 10.30564/vsr.v1i1.879

Yang Song, J. Zhao, Hui-ming Yang, Yawen Bu, Guozhong Zhang

{"title":"Molecular Analysis of Pathogenicity Differences of Avian Paramyxovirus 1 Genotypes VI and VII in Chickens","authors":"Yang Song, J. Zhao, Hui-ming Yang, Yawen Bu, Guozhong Zhang","doi":"10.30564/vsr.v1i1.879","DOIUrl":"https://doi.org/10.30564/vsr.v1i1.879","url":null,"abstract":"Background: Genotypes VI and VII of (APMV-1) have different host range and pathogenicity in pigeons and chickens. However, the molecular determinants of these differences are still unclear. Methods: Here, we aligned the DNA sequences of 56 genotype VI and 33 genotype VII APMV-1 strains. Sequence alignment results revealed that there are 17 amino acids sites differed between APMV-1 strains of these two genotypes. We then constructed a plasmid based on the full-length genome of rSG10 APMV-1 strain, which belongs to genotype VII but was mutated with these 17 VI-genotype-specific amino acids, and rescued as rSG10-17 strain. The restriction digestion and ligation and overlapping PCR methods were used in the construction of plasmids with amino acids mutation. This virus was evaluated for its virulence and growth characteristics. Results and conclusion: The results indicated that the virulence and the growth characteristics have no obvious difference between the rSG10-17 virus and its parental strain rSG10. The simultaneous mutation of 17 genotype-specific amino acids did not affect the virulence of APMV-1 in chickens. Further analysis of these amino acids is required by taking into consideration of the functions of encoded proteins.","PeriodicalId":17588,"journal":{"name":"Journal of Veterinary Science and Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75229366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0