禽副粘病毒1基因型VI和VII对鸡致病性差异的分子分析

Yang Song, J. Zhao, Hui-ming Yang, Yawen Bu, Guozhong Zhang
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摘要

背景:APMV-1基因型VI和VII在鸽子和鸡中具有不同的宿主范围和致病性。然而,这些差异的分子决定因素仍不清楚。方法:对56株VI基因型和33株VII基因型APMV-1进行DNA序列比对。序列比对结果显示,两种基因型APMV-1株存在17个氨基酸位点的差异。我们利用rSG10 APMV-1菌株的全基因组构建了质粒,该菌株属于VII基因型,但被这17个vi基因型特异性氨基酸突变,并被拯救为rSG10-17菌株。构建氨基酸突变质粒采用限制性内切连接法和重叠PCR法。对该病毒的毒力和生长特性进行了评价。结果与结论:rSG10-17病毒的毒力和生长特性与亲本株rSG10无明显差异。17个基因型特异性氨基酸同时突变对鸡APMV-1的毒力没有影响。考虑到编码蛋白质的功能,需要对这些氨基酸进行进一步分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Analysis of Pathogenicity Differences of Avian Paramyxovirus 1 Genotypes VI and VII in Chickens
Background: Genotypes VI and VII of  (APMV-1) have different host range and pathogenicity in pigeons and chickens. However, the molecular determinants of these differences are still unclear. Methods: Here, we aligned the DNA sequences of 56 genotype VI and 33 genotype VII APMV-1 strains. Sequence alignment results revealed that there are 17 amino acids sites differed between APMV-1 strains of these two genotypes. We then constructed a plasmid based on the full-length genome of rSG10 APMV-1 strain, which belongs to genotype VII but was mutated with these 17 VI-genotype-specific amino acids, and rescued as rSG10-17 strain. The restriction digestion and ligation and overlapping PCR methods were used in the construction of plasmids with amino acids mutation. This virus was evaluated for its virulence and growth characteristics. Results and conclusion: The results indicated that the virulence and the growth characteristics have no obvious difference between the rSG10-17 virus and its parental strain rSG10. The simultaneous mutation of 17 genotype-specific amino acids did not affect the virulence of APMV-1 in chickens. Further analysis of these amino acids is required by taking into consideration of the functions of encoded proteins.
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