Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)最新文献_第8页

An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant GRA1 protein for detection of igg antibodies againts Toxoplasma gondii infections 重组gr1蛋白酶联免疫吸附法检测刚地弓形虫igg抗体的评价研究

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/jmedsci005102201904

Nina Difla Muflikhah, W. Artama

引用次数: 0

The role of genital tract infection inpreterm delivery: aretrospective study 生殖道感染在早产中的作用:一项回顾性研究

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/jmedsci005103201904

Yohanes Aditya Adhi Satria, Uki Retno Budihastuti, T. Susilawati

{"title":"The role of genital tract infection inpreterm delivery: aretrospective study","authors":"Yohanes Aditya Adhi Satria, Uki Retno Budihastuti, T. Susilawati","doi":"10.19106/jmedsci005103201904","DOIUrl":"https://doi.org/10.19106/jmedsci005103201904","url":null,"abstract":"Preterm delivery is associated with higher mortality and morbidity of neonates, also increasestheir risk of having growth and development impairment.This study aimed toidentify the role ofgenital tract infection in preterm delivery. A retrospective study was conducted on medical records of mothers who had preterm delivery in a tertiary hospital in Surakarta, Indonesia during 2017. The data collected were mothers’ age and their gestational age, the history of current pregnancy, the number of previous abortion(s), mothers’ body temperature, the extent of abnormal vaginal discharge, and laboratory findings (white blood cell count, platelet count, red blood cell count, hemoglobin count, hematocrit level, urinalysis and microbiology results). The statistical differences amongst categorical and numerical data were analyzed using the Chi-Square test and the Mann-Whitney test. Based on the patient’s history and the examination results, we suspected genital tract infections in22.52% (25/111)of subjects. All of them had abnormal vaginal discharge despite only one case had been confirmed as streptococcal infection. This study found that the majority of mothers with probable genital tract infection hadpreterm premature rupture of the membrane whilst preeclampsiawas more evident in those without genital tract infection. We conclude that genital tract infection during pregnancy is a significant contributor to the occurence of premature birth so that microbiological testing is needed to confirm the diagnosis.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85138375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective role of single bulb garlic ( Allium sativum L.) towards foam cells and vascular cell adhesion molecules-1 (VCAM-1) on mice ( Mus musculus ) with high- fat diet 大蒜对高脂饮食小鼠泡沫细胞和血管细胞粘附分子-1的保护作用

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/jmedsci005103201905

Isfatun Chasanah, A. Ghofur, S. Lestari

{"title":"The protective role of single bulb garlic ( Allium sativum L.) towards foam cells and vascular cell adhesion molecules-1 (VCAM-1) on mice ( Mus musculus ) with high- fat diet","authors":"Isfatun Chasanah, A. Ghofur, S. Lestari","doi":"10.19106/jmedsci005103201905","DOIUrl":"https://doi.org/10.19106/jmedsci005103201905","url":null,"abstract":"Atherosclerosis is initiated by inflammatory response characterized by the accumulation of lipids in the arteries, causing blockage of blood vessels. The excessive of low-density lipoprotein (LDL) in the blood is a major factor in endothelial dysfunction and results in an inflammatory process. The inflammatory process in atherosclerosis is due to the expression of adhesion molecules, vascular cell adhesion molecules-1 (VCAM-1). This research aimed to evaluate the effect of single bulb garlic oil extract (SGBOE) against VCAM-1 expression in high-fat diet mice. Thirty male mice strain Balb/C were acclimatized for one week. Mice were divided into 6 groups (n=5): normal, high-fat diet (HFD), HFD + simvastatin, and HFD + SBGOE 12.5, 25, and 50 mg/kg BW respectively. HFD was given for 45 days. The SGBOE was given for four weeks orally. The aortic VCAM-1 expression was assayed using the immunohistochemical-flouresencemethod. The result of One-Way ANOVA showed that the treatment influenced significantly (p<0.05). The foam cells increase in the high-fat diet group compared than normal mice. Dose 12,5 mg/kg BW reduces VCAM-1 expression near the normal group. Allicin and S-allyl cysteine (SAC) compounds in single bulb garlic are antioxidant compounds that act to prevent oxidative stress and prevention mechanisms against lipoprotein modification.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88438708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular endothelial growth factor (VEGF) serum levelsof patients with Helicobacter pylori gastritis with cytotoxin-associated gene A positive (CagA + )status 细胞毒素相关基因A阳性(CagA +)状态幽门螺杆菌胃炎患者血清血管内皮生长因子(VEGF)水平

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/jmedsci005104201907

Wira Prihatin Siregar, G. Siregar, T. Sungkar

{"title":"Vascular endothelial growth factor (VEGF) serum levelsof patients with Helicobacter pylori gastritis with cytotoxin-associated gene A positive (CagA + )status","authors":"Wira Prihatin Siregar, G. Siregar, T. Sungkar","doi":"10.19106/jmedsci005104201907","DOIUrl":"https://doi.org/10.19106/jmedsci005104201907","url":null,"abstract":"Helicobacter pylori ( H.pylori ) is an agent that causes gastritis and the ulcer of gaster, which are ultimately caused gastric tumors. The prevalence is about 80 % average in developing country and 20-50 % in developed countries. One of the virulence factor iscytotoxin-associated gene A (CagA) that plays a role in the inflammation process,cell proliferation and metaplasia in gastric mucosa. Vascular endothelial growth factor (VEGF)is one of angiogenic factors that plays a role in the process of making new mucosal tissue after the inflammation by H.pylori . The escalation of VEGF expression levels contribute to the beginning of gastric carcinogenesis. The studywas aimed to analyze of VEGF serum levels between CagA(+) and CagA(-) in patients with H.pylori gastritis. Cross sectional study was conducted on 30 patients with H.pylori gastritis after conducted gastroscopy,biopsy and CLO test, which were continued with VEGF serum examination using ELISA test and perfomed PCR test to determinethe CagA status.The data was analyzed with SPSS 22 version andp value <0.05 was considered significant.The results of this study were18 men (60 %) and 12 women (46%) from 30 total subjects, with a median age average was 53.5 years old.Majority ethnic was Batak with 16 subjects (53.3%). H elicobacter pylori gastritis with CagA(+) was about 21 subjects (70%) and H.pylori gastritis with CagA(-) was about 9 subjects (30%). We found that median VEGF serum levels of patients with H.pylori gastritis with CagA(+)[480.3 pg/dL (115.5-2185.2)] significantly higher compared to thatwith CagA(-) [291.1 pg/dL (158.4-556.7)] (p<0.05).In conclusion, the VEGF serum levels ofpatients with H.pylori gastritis with CagA(+) is higher compared to that withCagA(-).","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78264488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between hyperuricemia and erectile dysfunction on hypertension patients 高血压患者高尿酸血症与勃起功能障碍的关系

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/JMEDSCI005102201907

J. Ticoalu, B. I. Kandarina, D. M. Rizal, I. D. Putu, Pramantara Setiabudi

{"title":"Relationship between hyperuricemia and erectile dysfunction on hypertension patients","authors":"J. Ticoalu, B. I. Kandarina, D. M. Rizal, I. D. Putu, Pramantara Setiabudi","doi":"10.19106/JMEDSCI005102201907","DOIUrl":"https://doi.org/10.19106/JMEDSCI005102201907","url":null,"abstract":"Hypertension is a major non-communicable disease worldwide including in Indonesia. It can cuase erectile dysfunction through vasculogenic pathway. Uric acid level could be a promising biomarker to predict erectile dysfunction due to it is related to endothelial dysfunction, microvascular disease, and hypertension. The aim of this study was to evaluate the relationship between hyperuricemia and erectile dysfunction on hypertension patients. This was an observational study with cross-sectional design involving 88 male hypertension patients aged more than 18 years who registered as outpatient in the Bethesda Lempuyangwangi Public Hospital, Yogyakarta. Hyperuricemia was measured using uricase method and erectile dysfunction was measured using IIEF-5 form. The results showed that hyperuricemia (OR=3.89; CI 95% 1.08-15.70; p=0.017), blood pressure (OR=6.84; CI 95% 2.35-20.6; p<0.001), and age (p<0.001) are related with erectile dysfunction on the hypertension patients. Furthermore, logistic regression analysis showed that hyperuricemia, age, and blood pressure simultaneously affect the erectile dysfunction occurance, with good calibration (p=0.167) and discriminative level (0.8604). In conclusion, there is significantly relationship between hyperuricemia and erectile dysfunction on hypertension patients.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82366463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mode of action of tocotrienol as anticancer 生育三烯醇的抗癌作用模式

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/jmedsci005102201909

Widiyasri Lodu, F. Karwur

引用次数: 1

Aloe vera stimulate cell proliferation, cell migration, expression of vascular endothelial growth factor-A (VEGF-A), and c-Jun N-terminal kinase-1 (JNK-1) on fibroblast of diabetic rat models 芦荟刺激糖尿病大鼠模型成纤维细胞增殖、细胞迁移、血管内皮生长因子- a (VEGF-A)和c-Jun n -末端激酶-1 (JNK-1)的表达

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/jmedsci005102201903

Insanul Firdaus, N. Arfian, M. Wahyuningsih, Denny Agustiningsih

{"title":"Aloe vera stimulate cell proliferation, cell migration, expression of vascular endothelial growth factor-A (VEGF-A), and c-Jun N-terminal kinase-1 (JNK-1) on fibroblast of diabetic rat models","authors":"Insanul Firdaus, N. Arfian, M. Wahyuningsih, Denny Agustiningsih","doi":"10.19106/jmedsci005102201903","DOIUrl":"https://doi.org/10.19106/jmedsci005102201903","url":null,"abstract":"The disturbance of cell migration and cell proliferation,diminished production of vascular endothelial growth factor-A (VEGF-A) and c-Jun N-terminal kinase-1 (JNK-1) are important factors in wound healing process. Aloe vera contains active compounds which can help in the wound healing process. Thestudy aimed to investigate the effect of ethanol extract ofA. veraon cell proliferation, cell migration, VEGF-A and JNK-1 expression of skin fibroblast cells of diabetic rats. The primary skin fibroblast cells were isolated from diabetic Wistar rat and incubated with the A. vera extract in various concentrations i.e. 500 (AV500), 250 (AV250), and 125 µg/Ml (AV125) for 24, 48 and 72 h.The cell proliferation was examined visually by counting the cells number, the cell migration was observed using in vitro scratch assay, whereas VEGF-A and JNK-1 expression were examined using RT-PCR. In 24 and 48h incubation,the cell proliferation of AV500 and AV250 groups had higher number of cells than negative control group,but there was no significant difference (p>0.05). However in72 h incubation,the cell proliferation of AV500 group (29.33±1.28x104 cells/mL)was significantly different compared to negative control group (22.91±3.21x104 cells/mL) (p 0.05).The expression of VEGF-A and JNK-1 after incubation with the AV500 for 48 h, weresignificantly higher than those of negative control group (p<0.05).In conclusion, A. vera increases cell proliferation, cell migration, VEGF-A and JNK-1 expression offibroblast cellof diabetic rat skin.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88267431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The eyelid tumor in Yogyakarta, Indonesia 印度尼西亚日惹的眼睑肿瘤

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/JMEDSCI005103201906

Agus Supartoto, Annisa Nurina Ayuningtyas, B. Dibyasakti, Purjanto Tepo Utomo, Datu Respatika, M. B. Sasongko

{"title":"The eyelid tumor in Yogyakarta, Indonesia","authors":"Agus Supartoto, Annisa Nurina Ayuningtyas, B. Dibyasakti, Purjanto Tepo Utomo, Datu Respatika, M. B. Sasongko","doi":"10.19106/JMEDSCI005103201906","DOIUrl":"https://doi.org/10.19106/JMEDSCI005103201906","url":null,"abstract":"Many types of eyelid tumors may easily be diagnosed from a clinical point of view. However only a small number of large studies exist exploring the frequency of eyelid lesions from different regions. According to the epidemiology of various eyelid lesions happened in Yogyakarta Special Region, this study was aimed to investigate the prevalence of eyelid tumors in Yogyakarta Special Region. A total of 94 patients were enrolled in a descriptive study. The data were taken retrospectively from the medical record. All patients were diagnosed with eyelid tumors from January 2014 until December 2017 by histopathological examination. Among the subjects, 56 (59.6%) were male and 38 (40.4%) were female. There was no difference found in laterality (OD 46% vs OS 50%). Sebaceous carcinoma was found in 15 (16%) patients, followed by squamous cell carcinoma (SCC) 13 (13.8%), basal cell carcinoma (BCC) 11 (11.7%), epidermoid cyst 7 (7.4%), non-Hodgkin lymphoma 7 (7.4%), and others. We also found that 11 (11.7%) of patients showed an inflammation appearance only. The therapy was varied from extirpation and biopsy (39.4%), wide excision (27.7%), excision and biopsy (18.1%), exenteration (10.6%), and also anterior and lateral orbitotomy (2.1%) for each procedure. The eyelid tumor was found equally in the right and the left eye. Sebaceous carcinoma followed by SCC and BCC were the most common eyelid tumor found in this study. A further study is needed to determine the risk factor of each tumor.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89787685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Solitary dermal cylindroma: a rare case report 孤立性皮肤圆筒状瘤1例

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI: 10.19106/JMEDSCI005102201910

W. Widayati, Irianiwati

{"title":"Solitary dermal cylindroma: a rare case report","authors":"W. Widayati, Irianiwati","doi":"10.19106/JMEDSCI005102201910","DOIUrl":"https://doi.org/10.19106/JMEDSCI005102201910","url":null,"abstract":"Dermal cylindromas are rare benign skin appendageal tumors that has two clinical presentation, solitary and multiple form. The diagnosis is unusual.However, it must be considered in clinical practice.A 51-year-old woman hadpainless nodule on right arm. Skin examinatipn revealedtwo fragmented nodules, 1x0.5x0.5cm, tan to white, and rubbery. Histopathological findings revealed a well demarcated and an unencapsulated epithelial dermal tumor, composed of numerous oval and polygonal nests molded into a \"jig-saw\" or \"mosaic\" appearance at low power. The nests of cells were composed of basaloid cells with scant cytoplasm and hyperchromatic nuclei and paler cells at the center. Nests of epithelial cells were surrounded and penetrated by a thickened band of basement membrane material that was PAS-positive. Immunohistochemistry (IHC) staining with p63 showed positive staining on basaloid cells. Langerhans dendritic cells in the surrounding nests stained positive with CD1a. Solitary dermal cylindroma occurred sporadically in patients without family history of cutaneous cylindromas.Combination of morphologic, histochemystry and immunohistochemystry staining were needed for accurate diagnosis.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87418262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Polyherbal formulation containing Saoropus androgynous, Trigonella foenum-graceum, and Moringa oleifera increased the expression of mRNA smooth muscle α-actin (ACTA2) and cytokeratin 14 (CK14) in lactating rats 含有雌雄同体山参、三角茅和辣木的复方可提高泌乳大鼠平滑肌α-肌动蛋白(ACTA2)和细胞角蛋白14 (CK14) mRNA的表达

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-05-29 DOI: 10.19106/JMEDSCI005102201902

F. S. Yuliani, S. Purwono, A. Sadewa, D. S. Heriyanto, Rahmaningsih Mara Sabirin, Mustofa

{"title":"Polyherbal formulation containing Saoropus androgynous, Trigonella foenum-graceum, and Moringa oleifera increased the expression of mRNA smooth muscle α-actin (ACTA2) and cytokeratin 14 (CK14) in lactating rats","authors":"F. S. Yuliani, S. Purwono, A. Sadewa, D. S. Heriyanto, Rahmaningsih Mara Sabirin, Mustofa","doi":"10.19106/JMEDSCI005102201902","DOIUrl":"https://doi.org/10.19106/JMEDSCI005102201902","url":null,"abstract":"Polyherbal formulation (PHF) containing extracts of Sauropus androgynous, Trigonella foenum-graceum and Moringa oleifera has been proven can induce milk production in animal model. However, its molecular of action has not been elucidated, yet. This study aimed to investigate the effect of the PHF on the mRNA expressions of α-actin smooth muscle (ACTA2) and cytokeratin 14 (CK14) on the myoepithelial cells of the lactating rats mammary glands. Thirty female Wistar rats were divided into five groups with six of each. Group I was orally administered aquadest. Group II, III, and IV rats were orally administered the PHF at dose level of 26.25, 52.5, and 105 mg/kg once a day, for 15 days, respectively. Group V was orally administered 2.7 mg domperidone. On 16th day, rats were sacrificed. Mammary glands were isolated and processed for mRNA expression analysis using real-time polymerase chain reaction (qRT-PCR). The results demonstrated that the mRNA expression of ACTA2 and CK14 increased in dose-dependent manner in the groups of PHF. Significantly different between the Group III, IV, and V compared to Group I was observed (p 0.05). In conclusion, the PHF increases the mRNA expression of ACTA2 and CK14 on myoepithelial cells of the mammary glands on lactating rats.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90370830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2