重组gr1蛋白酶联免疫吸附法检测刚地弓形虫igg抗体的评价研究

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran) Pub Date : 2019-07-02 DOI:10.19106/jmedsci005102201904

Nina Difla Muflikhah, W. Artama

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引用次数: 0

摘要

可靠的实验室检测对检测刚地弓形虫感染很重要，重点是改进低成本和易于使用的诊断仪器。酶联免疫吸附测定法(ELISA)是一种在抗体或抗原检测的基础上，可在短时间内测定大量样品的方法。采用ELISA法检测作为抗原的GRA1蛋白对弓形虫病诊断的敏感性和特异性，并与市售ELISA试剂盒进行比较。采集70份血清样本，采用间接ELISA、商用ELISA试剂盒和GRA1蛋白包被抗原进行检测。ELISA- gra1和市售ELISA试剂盒检测结果分别为48份和51份IgG抗体阳性。ELISA检测GRA1的敏感性为100%，特异性为86.36%。阳性预测值(PPV)为94.11%。结果表明，重组蛋白GRA1是人弓形虫病的高免疫原性蛋白，是弓形虫病筛查的标记物。

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An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant GRA1 protein for detection of igg antibodies againts Toxoplasma gondii infections

Reliable laboratory testing is important to detect Toxoplasma gondii infection and focuses on improving the low cost and easy to use the diagnostic instrument. Enzyme-linked immunosorbent assay (ELISA) method can be used to determine a large number of samples within a short period of time which based on antibody or antigen detection. We tested the sensitivity and specificity of GRA1 protein of as antigen using the ELISA method to toxoplasmosis diagnosis and compared it with commercial ELISA kit. Seventy sera samples were collected and tested using indirect ELISA, commercial ELISA kit and GRA1 protein-coated as antigen. The results showed 48 and 51 samples had positive IgG antibody using ELISA-GRA1 and commercial ELISA kit, respectively. The GRA1 sensitivity and specificity on ELISA were 100% and 86.36%, respectively. Whereas positive predictive value (PPV) was 94.11%. This result indicated that the recombinant GRA1 protein is a highly immunogenic protein in human toxoplasmosis and marker for toxoplasmosis screening.

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Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)

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