Journal of Translational Medicine最新文献

{"title":"CPMOAD: comprehensive preeclampsia multi-omics analysis database.","authors":"Yang Fang, Mengzhe Fan, Hao Li, Hongen Xu, Hongmei Du, Yaqing Guo, Jinshuang Gao, Erfeng Yuan, Liying Song, Yu Wang, Qianqian Shi, HaiYang Yu, Enwu Yuan, Xin Zhao, Linlin Zhang","doi":"10.1186/s12967-025-07094-x","DOIUrl":"10.1186/s12967-025-07094-x","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia (PE) is a pregnancy-specific syndrome that poses serious risks to both maternal and fetal health globally. Despite the accumulation of multi-omics datasets from various studies, a unified platform that integrates these resources for comprehensive analysis remains unavailable.</p><p><strong>Methods: </strong>We developed CPMOAD (Comprehensive Preeclampsia Multi-Omics Analysis Database), an online platform designed to integrate RNA-seq, DNA methylation, and literature-curated data related to PE. All datasets were uniformly processed to generate differential expression profiles, methylation data, and curated molecular interaction records. The database is equipped with interactive visualization tools and customizable analysis modules.</p><p><strong>Results: </strong>CPMOAD offers access to a broad spectrum of PE-relevant data, including gene expression and methylation profiles. Users can perform differential expression analysis, methylation analysis, and pathway enrichment analysis directly through the web interface. The platform also enables the exploration of validated molecular signatures associated with PE.</p><p><strong>Conclusions: </strong>CPMOAD serves as a comprehensive and user-friendly resource for integrated multi-omics analysis in preeclampsia research. By consolidating diverse datasets and providing powerful analytical tools, CPMOAD aims to accelerate the discovery of molecular mechanisms and potential biomarkers for PE. The database is freely accessible at http://nscc.zzu.edu.cn/CPMOAD/ .</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1026"},"PeriodicalIF":7.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA methylation in hepatocellular carcinoma: from metabolic reprogramming and immune escape mechanisms to small molecule inhibitor development.","authors":"Yang Xu, Fei Lan, Chenguang Yang, Pengfei Li","doi":"10.1186/s12967-025-07026-9","DOIUrl":"10.1186/s12967-025-07026-9","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) is a primary liver malignancy characterized by a high mortality rate and unfavorable prognosis. Altered epigenetic modifications have been closely associated with cancer development and tumor immune escape. RNA methylation is a pervasive epigenetic alteration. N6-methyladenosine (m6A), 5-methylcytosine (m5C), N1-methyladenosine (m1A), N7-methylguanosine (m7G), 3-methylcytidine (m3C), pseudouridine (Ψ), and 2'-O-methylation (Nm) are the main types of RNA methylation. Importantly, abnormal RNA modifications in HCC are key drivers in promoting the translation of oncogenic RNA transcripts. This not only provides cancer cells with a growth-promoting edge but also significantly contributes to tumorigenesis, fueling processes such as uncontrolled cell proliferation, invasion, and metastasis. RNA methylation influences metabolic reprogramming, immune cells, and immunological factors by modulating biological processes like RNA splicing, translation, stability, and translocation. Consequently, RNA methylation is pivotal in modulating biological processes including HCC tumor immunity, proliferation, invasion, and metastasis. This paper systematically examines the mechanisms and functions of these seven types of RNA methylations, offering a thorough overview of their roles and probable mechanisms within the HCC tumor microenvironment and immune system. We seek to offer novel insights and ways to enhance the effectiveness of HCC immunotherapy.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1022"},"PeriodicalIF":7.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZKSCAN5 transcriptional regulation of APOC1 modulates ferroptosis via PI3K/AKT/SREBP2/SLC1A5 axis.","authors":"Yongbo Liu, Zihao Qi, Shuo Yang, Yanze Li, Jia Guo","doi":"10.1186/s12967-025-07092-z","DOIUrl":"10.1186/s12967-025-07092-z","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer is a great substantial health challenge among the cancer type with a high incidence and serving as the main cause of cancer-related deaths in men. Apolipoprotein C1 encodes a member of the apolipoprotein C family. The APOC1 has been confirmed as an oncogene of prostate cancer. However, the mechanism of how the APOC1 protein influence remains to be elucidated.</p><p><strong>Methods: </strong>The expression of APOC1 was detected in both prostate cancer tissues and prostate cancer cell lines. The APOC1 knockdown and overexpression cell models were created. The effect of APOC1 on prostate cancer cell proliferation,metastasis, EMT and ferroptosis were explored by colony formation, wound healing,transwell assays, CCK-8 and western blotting in vitro and subcutaneous tumor formation in nude mice. Furthermore, the mechanism of how APOC1 inhibits ferroptosis in prostate cancer through PI3K/AKT/SREBP2/SLC1A5 was detected. Meanwhile, the interaction of APOC1 and ZKSCAN5 (zinc finger with KRAB and SCAN domains 5) was determined using Chromatin Immunoprecipitation (ChIP).</p><p><strong>Results: </strong>APOC1 expression was significantly upregulated in prostate cancer tissues and cell lines. Genetic silencing of APOC1 by shRNA demostrated potent tumor-suppressive effects, markedly inhibiting cell proliferation, metastasis and EMT, while concurrently enhancing ferroptosis rates. Then, APOC1 was shown to modulate cholesterol homeostasis via the PI3K/AKT/SREBP2/SLC1A5 signaling cascade, thereby influencing ferroptosis susceptibility in prostate cancer cells. Mechanistically, ZKSCAN5 was identified as a transcriptional repressor of APOC1 through direct promoter binding. Notably, the anti-ferroptosis function of APOC1 was mediated through SREBP2-dependent transcriptional regulation, with Cut&Tag (Cleavage Under Targets and Tagmentation) confirming SREBP-2's direct binding to the SLC1A5 promoter.</p><p><strong>Conclusion: </strong>In prostate cancer, APOC1 regulates ferroptosis via PI3K/AKT/SREBP2/SLC1A5 axis, meanwhile ZKSCAN5 negatively regulates the expression of APOC1.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1020"},"PeriodicalIF":7.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and experimental study of potential biomarkers for ulcerative colitis based on weighted gene co-expression network analysis and machine learning.","authors":"Zepeng Chen, Xingchen Wang, Changfang Xiao, Yongqing Cao","doi":"10.1186/s12967-025-07058-1","DOIUrl":"10.1186/s12967-025-07058-1","url":null,"abstract":"<p><strong>Background: </strong>Ulcerative colitis (UC) is a chronic nonspecific inflammatory intestinal disease affecting the mucosa and submucosa, characterized by continuous and diffuse active inflammation. However, its underlying pathogenesis remains unclear.</p><p><strong>Objective: </strong>This study aimed to identify potential UC biomarkers by integrating weighted gene co-expression network analysis (WGCNA) with machine learning, followed by validation in an experimental UC mouse model.</p><p><strong>Methods: </strong>The Gene Expression Omnibus database was systematically queried, and the GSE87466 dataset, comprising of colonic tissues from 87 patients with UC and 21 healthy controls, was retrieved. Differentially expressed genes (DEGs) were identified and subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. WGCNA was used to extract UC-related DEGs. Two machine learning algorithms, the Least Absolute Shrinkage and Selection Operator (LASSO) and Support Vector Machine Recursive Feature Elimination (SVM-RFE), were used to screen potential biomarkers. These biomarkers were then validated using animal experiments.</p><p><strong>Results: </strong>A total of 1,097 DEGs were identified. WGCNA constructed nine co-expression gene modules, with the turquoise module (520 genes) exhibiting the highest relevance to UC. LASSO and SVM-RFE analysis identified poly(ADP-ribose) polymerase family member 8 (PARP8) as a potential biomarker of UC. Immunological analysis revealed significantly higher proportions of naive B cells, activated CD4⁺ memory T cells, follicular helper T cells, γδT cells, M0 macrophages, M1 macrophages, activated mast cells, and neutrophils in UC samples compared to controls. PARP8 expression positively correlated with neutrophils, M1 macrophages, and activated CD4⁺ T cells, but negatively correlated with plasma cells. In vivo validation confirmed elevated PARP8 expression in dextran sulfate sodium-induced UC mice compared to controls.</p><p><strong>Conclusion: </strong>PARP8 may contribute to UC pathogenesis via immune-related pathways and holds promise as a diagnostic and predictive biomarker.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1029"},"PeriodicalIF":7.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles for targeted drug delivery: advances in surface modification strategies and therapeutic applications.","authors":"Minghui Hu, Yuxin Han, Xin Zhang, Shengqian Tian, Zhenghui Shang, Zhixiang Yuan, Lili He","doi":"10.1186/s12967-025-07077-y","DOIUrl":"10.1186/s12967-025-07077-y","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) refer to a heterogeneous group of cell-derived membrane structures originating from endosomes or plasma membranes. EVs can be used as excellent medication carriers due to their characteristic as natural carriers for bioactive substances. They can be employed for intercellular communication to transport bioactive lipids, proteins, RNAs, DNA, and peptide segments for releasing into the extracellular surroundings. This paper discusses the biological characteristics of EVs, current methodologies for their isolation and purification, with particular emphasis on emerging strategies for EVs surface engineering. The methods for surface modification can be categorized into two approaches: pre-isolation and post-isolation. Pre-isolation methods involve genetic manipulation or metabolic engineering of the source cells to alter the EVs surface, while post-isolation methods entail physical or chemical modifications after EVs have been isolated. Based on the modification methods mentioned above, we present how these modified EVs can be utilized to treat specific diseases, including brain disorders, tumors, and liver conditions.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1028"},"PeriodicalIF":7.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of metabolic markers in pediatric epilepsy disorders.","authors":"Yu Chen, Shuo Kong, Yuhui Wang, Yansheng Ding, Biwen Peng, Jian Xu","doi":"10.1186/s12967-025-06917-1","DOIUrl":"10.1186/s12967-025-06917-1","url":null,"abstract":"<p><strong>Background: </strong>The ketogenic diet (KD) has been shown to effectively reduce seizures. Ketogenic medium-chain triglycerides (MCTs) enhance brain energy metabolism in adults with neurological disorders, but their effect on peripheral metabolites in children with epilepsy is unclear. The function and mechanisms of MCTs in pediatric epilepsy remain poorly understood.</p><p><strong>Methods: </strong>We performed untargeted LC-MS metabolomics on blood samples from children with epilepsy (n = 14) and healthy controls (n = 20), identifying octanoic acid (OA) as significantly elevated. To explore its potential role, OA was administered in a PTZ-induced seizure model in juvenile mice. Behavioral analysis, Nissl staining, and immunofluorescence demonstrated that OA modulated seizure susceptibility and activated GPR40, a receptor expressed in neurons.</p><p><strong>Results: </strong>(1) A total of 518 metabolites were examined in plasma samples, with the top 20 metabolites selected, of which 14 were upregulated and 6 were downregulated. (2) In the PTZ-induced epilepsy model, OA significantly prolonged seizure latency by 59.86 ± 7.032 s (P < 0.0001), without affecting seizure grade or duration. Mechanistically, OA enhanced GPR40 activation and inhibited astrocyte activation in the hippocampus.</p><p><strong>Conclusion: </strong>This study identified and validated peripheral blood metabolites in children with epilepsy. OA was found to exert a protective effect via GPR40 activation, enhancing neuroprotection. These findings suggest that OA may play a critical role in neuroprotection and could serve as a potential therapeutic target for pediatric epilepsy.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1018"},"PeriodicalIF":7.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CMR-FT right atrial strain is a novel predictive indicator in left ventricular noncompaction patients: a multi-center study.","authors":"Rongxue Shan, Yan Gao, Ruopeng Wang, Runzhi Zhang, Jian Wang, Pengxi Han, Junhao Xu, Shifeng Yang, Congshan Ji, Xianshun Yuan, Ximing Wang","doi":"10.1186/s12967-025-07166-y","DOIUrl":"10.1186/s12967-025-07166-y","url":null,"abstract":"<p><strong>Objectives: </strong>Right atrial (RA) strain is increasingly recognized as a significant predictor of adverse events in patients with various cardiovascular conditions. However, the prognostic value of RA strain in patients with left ventricular noncompaction (LVNC) is unclear. The objective of this study was to evaluate the prognostic significance of RA strain derived from cardiac magnetic resonance feature tracking (CMR-FT) in patients with LVNC.</p><p><strong>Methods: </strong>394 LVNC patients who underwent CMR at 4 Chinese medical facilities from September 2014 to July 2023 were retrospectively and consecutively included in total. RA strain parameters were obtained using CMR-FT. Major adverse cardiac events (MACEs) were assessed, and all patients were followed up.</p><p><strong>Results: </strong>156 patients (39.6%) experienced MACEs during a median follow-up of 34 months. At univariable analysis, RA conduit strain was associated with MACE (hazard ratio [HR] 0.88 [95% CI 0.85-0.91]; P < 0.001). RA conduit strain maintained an independent predictor of MACE in a multivariate model that included left ventricular ejection fraction (LVEF) and late gadolinium enhancement (LGE) (HR 0.89 [95% CI 0.85-0.93]; p < 0.001). Furthermore, adding RA conduit strain to the multivariate model greatly enhanced the prognostic role of endpoint events (C-statistic improvement: 0.766-0.871, Delong test: p < 0.001). Net reclassification index (NRI) (0.201, p < 0.05) and integrated discrimination improvement (IDI) (0.038, p < 0.05) also showed the same trend.</p><p><strong>Conclusion: </strong>CMR-FT derived RA conduit strain is a potent independent indicator of major adverse cardiac events in left ventricular noncompaction patients. In addition, RA conduit strain can provide additional prognostic value over the multivariable baseline clinical model.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1016"},"PeriodicalIF":7.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of burden of topmost disorders requiring rehabilitation services: a systematic review and meta-analysis.","authors":"Echezona Nelson Dominic Ekechukwu, Olive Udunma Chijioke, Dorcas Tamilore Rotimi, Rajinder K Dhamija, Tochukwu Bright Ilechukwu, Paul Olowoyo, Amala Blessing Ojeh, Wuwei Feng, Solomon Chidubem Benjamin, Olumide Olasunkanmi Dada, Blessing Chiagozikam Atueyi, Thomas Platz, Mayowa Ojo Owolabi","doi":"10.1186/s12967-024-05987-x","DOIUrl":"10.1186/s12967-024-05987-x","url":null,"abstract":"<p><strong>Background: </strong>Rehabilitation is an essential health service that should be available for all with health conditions affecting functioning in daily life. Initiatives for equitable distribution of scarce and limited rehabilitation resources are frequently guided by burden of diseases measures. Most studies on burden of diseases have laid greater emphasis on disability adjusted life years (DALYs) and rarely compared these burdens among conditions. This present study aims to systematically review and compare the burden of the top 10 GBD ranking of disorders.</p><p><strong>Method: </strong>This review was pre-registered with PROSPERO (CRD42022316091). PubMed and Google Scholar were systematically searched as well as a manual search of grey literatures from 01/01/1990 to 29/02/2022. The results of this review were reported based on PRISMA guideline. Meta-analysis results were presented using forest plots and summary tables.</p><p><strong>Results: </strong>A total of 11,367 studies were obtained from the searches, while the findings of 55 studies (17,753,434 participants) were reviewed. Majority of the studies were conducted in high-income-countries (56.1%) though, all the studies on neonatal disorders (100.0%) and congenital birth disorders (100.0%) came from the low-and-middle-income-countries. Neonatal disorders, stroke and ischaemic heart disease (IHD) topped the rank of disease burden. Overall, the burden of neurological disorders and their associated risk factors (aRFs), in terms of disease prevalence, were evaluated to be 36.75% while their mortality rate was 29.90%. Neurological conditions and aRFs accounted for 61.83% of total economic burden.</p><p><strong>Conclusion: </strong>Neonatal disorders, stroke and IHD are the three most burdensome disorders. There is therefore need for greater focus of rehabilitation attention and resources in the coming decades.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1015"},"PeriodicalIF":7.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12462021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Silencing PinX1 enhances radiosensitivity and antitumor-immunity of radiotherapy in non-small cell lung cancer.","authors":"Jieping Qiu, Ying Xia, Yawei Bao, Jingjing Cheng, Lei Liu, Dong Qian","doi":"10.1186/s12967-025-07009-w","DOIUrl":"10.1186/s12967-025-07009-w","url":null,"abstract":"","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1017"},"PeriodicalIF":7.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quadrant asymmetry alteration of retinal degeneration in thyroid-associated ophthalmopathy.","authors":"Weijie Liu, Wei Rao, JunYe Zhu, Xiaozhou Hu, Yunhai Tu, Wencan Wu, Jie Ye","doi":"10.1186/s12967-025-07043-8","DOIUrl":"10.1186/s12967-025-07043-8","url":null,"abstract":"<p><strong>Purpose: </strong>To measure alterations in quadrant asymmetry (QA) of retinal radial peripapillary capillary (RPC) density and to determine the association between subfield alterations and thyroid-associated ophthalmopathy (TAO).</p><p><strong>Methods: </strong>Optical coherence tomography angiography images of 51 control eyes and 80 TAO eyes were analyzed to quantify RPC density. QA was defined as the difference between the maximum and minimum RPC densities among four subfields. Comparisons of RPC QA between groups and the associations of QA with the occurrence of TAO were analyzed. Linear regression was conducted to determine the association of subfield alterations with visual field mean deviation (MD).</p><p><strong>Results: </strong>QA of RPC density in TAO eyes was higher than in control eyes (9.58 ± 2.40 vs. 6.70 ± 2.01, P < 0.001), although no significant difference was observed in global RPC density between the TAO and control groups (P = 0.395). QA of RPC density demonstrated a high area under the curve (AUC = 0.816) for detecting TAO, whereas the AUC of global RPC density was only 0.564. Among the four subfields, only nasal RPC density showed a significant decrease in TAO eyes (48.03 ± 2.56 vs. 50.19 ± 2.79, P < 0.001). Univariate linear regression revealed that nasal RPC density was correlated with visual field MD (standardized coefficient = 0.243, P = 0.005), whereas RPC density in the other three subfields did not show significant correlations (all P ≥ 0.082).</p><p><strong>Conclusion: </strong>Alterations in quadrant asymmetry of RPC density occurred in TAO, particularly decreased RPC density in the nasal subfield, which was significantly associated with visual field abnormalities in TAO.</p>","PeriodicalId":17458,"journal":{"name":"Journal of Translational Medicine","volume":"23 1","pages":"1014"},"PeriodicalIF":7.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12462272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}