{"title":"Polyphenolic constituents from the aerial parts of Thymus quinquecostatus var. japonica collected on ulleung island","authors":"I. Lee, J. Bae, Taewan Kim, O. Kwon, Tae Hoon Kim","doi":"10.3839/JKSABC.2011.124","DOIUrl":"https://doi.org/10.3839/JKSABC.2011.124","url":null,"abstract":"Phytochemical investigation on the polar constituents in the aerial parts of Thymus quinquecostatus var. japonica resulted in the isolation of three flavonoids along with four known phenolic components. The isolated compounds were characterized as 2(S)-5,7,3′,5′-tetrahydroxyflavanone (1), (+)-taxifolin (2), (+)-aromadendrin (3), rosmarinic acid (4), caffeic acid (5), protocatechuic acid (6), and protocatechuic aldehyde (7) from comparisons of their physicochemical and spectroscopic data 1H-, 13C-NMR, two-dimentioal NMR, and mass spectrometery) with those of authentic samples and reference data. This is the first report on the isolation of compounds 1–7 from T. quinquecostatus var. japonica. In addition, compounds 2 and 4 exhibited the most potent pancreatic lipase inhibitory activities in a concentration-dependent manner with IC50 values of 15.4±0.8 and 62.8±2.7 μM, respectively.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"75 1","pages":"811-816"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73373840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of hydroxycinnamoyl-coenzyme a Shikimate hydroxycinnamoyltransferase from Populus euramericana","authors":"B. Kim, In-Ah Kim, Joong-Hoon Ahn","doi":"10.3839/JKSABC.2011.125","DOIUrl":"https://doi.org/10.3839/JKSABC.2011.125","url":null,"abstract":"Hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase (HCT), PeHCT was cloned from Populus euramericana. The predicted amino acid sequence revealed that PeHCT contained a conserved HXXXDG motif that was found in acyltransferase. The in vitro substrate was determined using recombinant PeHCT. The recombinant PeHCT showed substrate-specificity towards shikimic acid. In addition, PeHCT displayed higher expression in the stem than in the leaf, and its expression increased during the growth stage of the leaf. Results indicate PeHCT could be involved in lignin biosynthesis by formation of p-coumaroyl shikimate.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"46 1","pages":"817-821"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81052896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Insecticidal effects of Tabebuia avellanedae-derived Constituent and its analogues against Nilaparvata lugens and Laodelphax striatellus","authors":"J. Jeon, Min-seok Oh, Hoi-Seon Lee","doi":"10.3839/JKSABC.2011.126","DOIUrl":"https://doi.org/10.3839/JKSABC.2011.126","url":null,"abstract":"Insecticidal activities of an active constituent derived from the bark of Tabebuia avellanedae and its analogues were examined using a micro-topical application bioassay against the brown planthopper, Nilaparvata lugens and the small brown planthopper, Laodelphax striatellus. The active constituent of T. avellanedae bark was characterized by various chromatographic methods and identified as 2-Hydroxy-3-(3-methyl-2-butenyl)-1,4-naphtoquinone. On the basis of 48 h lethal concentration 50 (LD50) values, the most toxic compound against N. lugens was 1,4-Naphtoquinone (0.042 μg/female), followed by 5,8-Dihydroxy-1,4-naphtoquinone (0.080 μg/female), 2-Hydroxy-3-(3-methyl-2-butenyl)-1,4-naphtoquinone (0.091 μg/female), and 2,3-Dichloro-1,4-naphtoquinone (0.186 μg/female). Similar results against L. striatellus were observed for 2-Hydroxy-3-(3-methyl-2-butenyl)-1,4-naphtoquinone and its analogues, except for 2,3-Dichloro-1,4-naphtoquinone and 5,8-Dihydroxy-1,4-naphtoquinone. These results indicate that T. avellanedae bark-derived material and its analogues have potential as new preventive agents for control of N. lugens and L. striatellus.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"311 1","pages":"822-826"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74804632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Redillas, J. Jeong, R. Strasser, Youn Shic Kim, Jukon Kim
{"title":"JIP analysis on rice (Oryza sativa cv Nipponbare) grown under limited nitrogen conditions","authors":"M. Redillas, J. Jeong, R. Strasser, Youn Shic Kim, Jukon Kim","doi":"10.3839/JKSABC.2011.127","DOIUrl":"https://doi.org/10.3839/JKSABC.2011.127","url":null,"abstract":"Nitrogen deficiency significantly reduces the CO2 assimilation capacity of plants and the quantum yield of photosynthesis. Here, we employed the JIP test to determine the effects of nitrogendeficiency on the plant’s photosysnthetic ability on the basis of chlorophyll fluorescence. Nitrogendeficient and nitrogen-replete rice plants were analyzed for the fluorescence transients of the plant leaves in comparison with the nitrogen-sufficient controls. Results showed that 7 day-replete plants behaved normally while 5, 3, and 1 day-replete plants were significantly affected from nitrogen starvation. More specifically, nitrogen starvation of plants resulted in an inactivation of photosystem II (PS II) reaction centers and a decline in electron transport beyond the reduced plastoquinone (QAA−), and a decrease in both the pool size and the reduction of end electron acceptors at the PS I. The affected plants were fully recovered from the deficiency after 7 days of nitrogen repletion, as evidenced by the similar level of fluorescence transients to the positive controls. Thus, our results demonstrated that the movement of electron carriers leading to the reduction of end electron acceptors was affected by nitrogen limitation leading to a more pronounced decrease in the reduction of end electron acceptors. Together with the fact that nitrogen-deficiency limits the CO2 assimilation of plants, this study indicates that nitrogen metabolism is tightly coupled with photosynthetic ability.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"459 1","pages":"827-832"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86683966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methanol extract of peony root (Peonia lactiflora) and its ethyl acetate fraction attenuate heart and brain injury in a rat model of ischemia-reperfusion","authors":"S. Lim, H. Han, J. Park, Jongwon Lee","doi":"10.3839/JKSABC.2011.121","DOIUrl":"https://doi.org/10.3839/JKSABC.2011.121","url":null,"abstract":"Methanol extract and its ethyl acetate fraction of peony root (Peonia lactiflora) were found to attenuate heart and brain injury in a rat model of ischemia-reperfusion. Thus, intake of peony root already approved for food in Korea could be beneficial for preventing myocardial and cerebral infarction caused by occlusion of coronary and cerebral arteries, respectively.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"81 1","pages":"799-801"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78968578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cadmium tolerance increased synergistically by heterologous co-expression of SpHMT1 and AtPCS1 in budding yeast","authors":"Sangman Lee","doi":"10.3839/JKSABC.2011.122","DOIUrl":"https://doi.org/10.3839/JKSABC.2011.122","url":null,"abstract":"Heterologous and simultaneous expression of Schizosaccharomyces pombe heavy metal tolerance factor 1 (SpHMT1) and Arabidopsis thaliana phytochelatin synthase (AtPCS1) showed synergistically increased tolerance to Cd in budding yeast. This finding suggests that the action of HMT1 in Cd detoxification is well correlated with that of PCS.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"9 1","pages":"802-805"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89836844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toxin Gene Profiling of Bacillus cereus Food Isolates by PCR","authors":"S. Seong, J. S. Lim, Kwang-geun Lee, Seogjae Lee","doi":"10.3839/JKSABC.2008.046","DOIUrl":"https://doi.org/10.3839/JKSABC.2008.046","url":null,"abstract":"Seventy-one Bacillus cereus strains (12 references and 59 food isolates) were analyzed for the occurrence of five different enterotoxin genes (nheABC, hblCDA, entFM, cytK, and bceT) and one emetic toxin cereulide synthetase gene (ces) by PCR (polymerase chain reaction). PCR analysis revealed eight toxigenic patterns in all B. cereus strains tested; they all carried both entFM and nheABC. The presence of hblCDA, cytK, and bceT varied according to the enterotoxin-producing strains, among which hblCDA was the least frequently detected in the food-isolated strains. Only five B. cereus strains harbored ces, associated with the emetic type of food poisoning; however, these strains were devoid of hblCDA, cytK, and bceT.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"111 1","pages":"263-268"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87750449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytochemical Constituents of the Roots of Erigeron annuus","authors":"N. Yoo, D. Jang, Jin-Sook Kim","doi":"10.3839/JKSABC.2008.053","DOIUrl":"https://doi.org/10.3839/JKSABC.2008.053","url":null,"abstract":"Seven compounds (1-7) were isolated from n-hexane and EtOAc-soluble fractions of the roots of Erigeron annuus by repeated silica gel column chromatography. They were identified as simiarenol (1), -sitosterol (2), daidzein (3), apigenin (4), apigenin 7-O--D-glucuronide (5), 3-hydroxy-pyran- 4-one (6), and -sitosterol glucoside (7) on the basis of physical and spectroscopic data. Compounds 1 and 3 were isolated for the first time from the Erigeron species.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"1 1","pages":"305-308"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85906570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saerom Park, Yu-Si Lee, Ji-Hyoung Ha, Ki-Hwan Park, Sook-yeon Lee, Youn-Ju Choi, Dong-Ho Lee, Sun-Hee Park, Kyung Ryu, Hyoung-Soo Shin, D. Bae, A. Kim, S. Ha
{"title":"Prediction of the Shelf-life of Chilled Foods at Various Temperatures","authors":"Saerom Park, Yu-Si Lee, Ji-Hyoung Ha, Ki-Hwan Park, Sook-yeon Lee, Youn-Ju Choi, Dong-Ho Lee, Sun-Hee Park, Kyung Ryu, Hyoung-Soo Shin, D. Bae, A. Kim, S. Ha","doi":"10.3839/JKSABC.2008.063","DOIUrl":"https://doi.org/10.3839/JKSABC.2008.063","url":null,"abstract":"This study was designed to estimate the shelf-life of the popular chilled foods kimbab (rice rolled in dried laver), samgak kimbab (triangular rice rolled in dried laver), eomook (fish surimi, boiled fish paste) mook (acorn-starch jelly), and tofu (soybean curd) in large discount markets and convenience stores. Different types of chilled foods were stored at 5, 7, and 10 for 7 and 28 days, and changes in the total numbers of the aerobic bacteria were monitored. Values of 6 and 7 log cfu/g were used as the standard. Ready-to-eat foods stored at 5 showed a much longer shelf-life compared to storage at 10. The respective percentage increases in the shelf-life observed at both 7 and 5 were kimbab (70%, 171%), samgak kimbab (87%, 143%), soybean curd (46%, 95%), fish surimi (46%, 99%), and mook (45%, 87%). To reduce the microbiological contamination, storages at 7 and 5 are recommended for the increases of 45-88 and 87-171% in the shelf-life of these chilled foods.","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"31 1","pages":"329-333"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82695419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Constituents of the seeds of Cornus officinalis with Inhibitory Activity on the Formation of Advanced Glycation End Products (AGEs)","authors":"G. Lee, D. Jang, Y. Lee, Youn-Chul Kim","doi":"10.3839/JKSABC.2008.055","DOIUrl":"https://doi.org/10.3839/JKSABC.2008.055","url":null,"abstract":"과육을 제거한 산수유 씨의 MeOH 추출물의 EtOAc 분획물로부터 이용하여 10종의 화합물을 분리하였다. 이들 중에서 (+)- pinoresinol, (-)-balanophonin, vanillin, 4-hydroxybenzaldehyde, coniferaldehyde, betulinic acid는 이 식물에서 처음으로 분리된 물질들이다. 본 연구에서 분리된 10종의 화합물에 대한 in vitro 최종당화산물(AGEs) 생성억제효능 실험결과, (-)-balanophonin (화합물 2)과 gallicin(화합물 3)이 각각 $IC_{50}$ 값 27.81과 18.04 ${mu}M$ 로 비교양성대조군인 aminoguanidine(974.59 ${mu}M$ )보다 훨씬 우수한 효능을 나타내었다. 【Ten compounds, (+)-pinoresinol (1), (-)-balanophonin (2), gallicin (3), vanillin (4), 4-hydroxybenzaldehyde (5), coniferaldehyde (6), betulinic acid (7), ursolic acid (8), 5-hydroxymethyl furfural (9), and malic acid (10), were isolated from a EtOAc-soluble fraction of the seeds of Cornus officinalis. The structures of these compounds were elucidated by spectroscopic methods as well as by comparison with reported values. Compounds 1, 2, and 4-7 were isolated from this species for the first time. All the isolates (1-10) were subjected to an in vitro bioassay to evaluate their inhibitory activity against advanced glycation end products (AGEs) formation. Among these, compounds 2 and 3 showed the significant inhibitory activity on AGEs formation with $IC_{50}$ values of 27.81 and 18.04 ${mu}M$ , respectively.】","PeriodicalId":17424,"journal":{"name":"Journal of the Korean Society for Applied Biological Chemistry","volume":"71 1","pages":"316-320"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76087047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}