美洲杨羟基肉桂酰基辅酶a - Shikimate羟基肉桂酰基转移酶的研究

Journal of the Korean Society for Applied Biological Chemistry Pub Date : 2011-10-01 DOI:10.3839/JKSABC.2011.125

B. Kim, In-Ah Kim, Joong-Hoon Ahn

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引用次数: 12

摘要

羟基肉桂酰基辅酶A (shikimate/quinate hydroxycinnamoyltransferase, PeHCT)是从欧美杨中克隆而来。预测的氨基酸序列显示，PeHCT含有一个保守的HXXXDG基序，该基序在酰基转移酶中发现。采用重组PeHCT法测定体外底物。重组PeHCT对莽草酸具有底物特异性。此外，PeHCT在茎中的表达量高于在叶中的表达量，且在叶的生长阶段表达量增加。结果表明，PeHCT可能参与木质素的生物合成，形成对香豆素酰莽草酸酯。

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Characterization of hydroxycinnamoyl-coenzyme a Shikimate hydroxycinnamoyltransferase from Populus euramericana

Hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase (HCT), PeHCT was cloned from Populus euramericana. The predicted amino acid sequence revealed that PeHCT contained a conserved HXXXDG motif that was found in acyltransferase. The in vitro substrate was determined using recombinant PeHCT. The recombinant PeHCT showed substrate-specificity towards shikimic acid. In addition, PeHCT displayed higher expression in the stem than in the leaf, and its expression increased during the growth stage of the leaf. Results indicate PeHCT could be involved in lignin biosynthesis by formation of p-coumaroyl shikimate.

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来源期刊

Journal of the Korean Society for Applied Biological Chemistry FOOD SCIENCE & TECHNOLOGY-FOOD SCIENCE & TECHNOLOGY

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审稿时长

4-8 weeks