NaUKMA Research Papers. Biology and Ecology最新文献

{"title":"Peculiarities of functioning of hematopoietic progenitor cells of bone marrow of patients with myelodysplastic syndrome in culture in vitro and in cell diffusion chambers in vivo","authors":"D. Bilko, M. Pakharenko","doi":"10.18523/2617-4529.2022.5.33-38","DOIUrl":"https://doi.org/10.18523/2617-4529.2022.5.33-38","url":null,"abstract":"Myelodysplastic syndrome is a clonal disease with changes that occur due to mutations that lead to cytogenetic disorders and involve the hematopoietic stem cell. Researchers have shown that colony growth types in leukemic cell culture with acute and chronic myeloid leukaemia are of a prognostic value to determine the likelihood of remission or disease progression. However, there is no single point of view on the prognostic value of clonal culture research methods in MDS. The aim of the study was to determine the role of hematopoietic progenitor cells, the closest descendants of stem cells, in the formation of the pathological process in MDS, and identify the features of colony formation and morphological composition of bone marrow colonies in patients in vitro and in vivo in diffusion chambers implanted in the abdominal cavity of linear animals to determine their possible prognostic value. Bone marrow samples from patients with MDS with an excess of blasts-1 were examined by haematological, cultural, cytological and statistical methods. The features of colony formation in culture with semi-liquid agar of bone marrow of patients with MDS with an excess of blasts-1 were revealed. It has been established that in patients with the disease detection, the colony-forming ability in the semi-liquid agar of progenitor cells is reduced, the number of cells in the colonies decreases, and the appearance of capricious forms of cell aggregates with changes in culture differentiation were detected. The ability of bone marrow in MDS with an excess of blasts-1 to form colony clones by hematopoietic progenitor cells when cultured in semi-liquid agar both in vitro and in vivo was evaluated. Informative indicators of morphofunctional characteristics of stem cells and their descendants in culture, such as colony-forming ability and cell content of colonies, were determined. It is proved that the results of cultivation in diffusion chambers in vivo are comparable with the data obtained in vitro, and can become, along with traditional methods, an additional sign of detection and progression of MDS.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124748849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of traditional and newly synthetized surface-active agents as a basis for the creation of new disinfectants","authors":"I. Furtat, O. Nechypurenko, P. Vakuliuk, Maryna Vortman, Valerii Shevchenko","doi":"10.18523/2617-4529.2022.5.25-32","DOIUrl":"https://doi.org/10.18523/2617-4529.2022.5.25-32","url":null,"abstract":"Aim. The main goal of the study was to investigate the antibacterial and fungicidal properties of the traditional and newly synthesized surfactants of different classes, compare the analysis of their antimicrobial activity and find out the prospects for further use for the creation of disinfectants. Methods. The biocidal activity of the studied cationic, anionic and nonionic surfactants was determined in relation to representatives of the species Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, as well as fungi Candida albicans. The detection of antibacterial and fungicidal effect and determination of minimum inhibitory concentrations (MIC) was carried out by cultivation of the test cultures of microorganisms in Nutrient Broth (NB, Himedia, India) for 24–48 hours at 37 °С in the presence of different concentrations of experimental surfactants (1000–0.1 ppm). The number of cells that remained viable after exposure to surfactants was determined by optical density in McFarland units, which were then converted to the number of cells in 1 ml using the standard McFarland scale. Results. The newly synthesized guanidine-containing oligomer at concentrations of 1000 and 100 ppm inhibited the growth of all test cultures without exception by 100 %. The biocidal effect on the representatives of various taxonomic groups of microorganisms differed in its effectiveness for various anionic surfactants (trilon B, surfactant 2 and surfactant 3) even at the highest concentration of 1000 ppm. In particular, only trilon B completely inhibited the growth of all test cultures. However, at the concentrations indicated above, the examined cationic (surfactant 1 and triethanolamine) and nonionic (OP-10 and triton X-100) exhibited a rather low antimicrobial activity. Conclusions. The results obtained indicate that the guanidine-containing oligomer exhibited the highest antimicrobial activity among the investigated surfactants. Therefore, it can be considered a promising surfactant for further use in the creation of broad-spectrum antimicrobial agents. Enhancement of the biocidal effect of newly created disinfectants can be implemented by introducing the studied anionic and nonionic detergents, and glutaraldehyde can be used to stabilize them or enhance their antimicrobial activity. Instead, the application of cationic surfactants used in the work is impractical.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125319833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coenopopulations of Isopyrum thalictroides L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine","authors":"Viktoriia Gritsenko, A. Gnatiuk, O. Rak, M. Gaponenko","doi":"10.18523/2617-4529.2022.5.44-54","DOIUrl":"https://doi.org/10.18523/2617-4529.2022.5.44-54","url":null,"abstract":"The article presents the results of a study of coenopopulations of European relict species, regionally rare ornamental spring ephemeroid Isopyrum thalictroides L. in the collection “Rare plants of the flora of Ukraine” and botanical-geographical areas “Altai and Western Siberia” and “Steppes of Ukraine” of M. M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine (NBG). The origin of I. thalictroides in the areas of the NBG is different. On the plot “Rare plants of the flora of Ukraine” I. thalictroides is an introduced species, but in the areas “Altai and Western Siberia” and “Steppes of Ukraine” it is ergasiophygophyte. In the areas of NBG I. thalictroides shows a tendency to expand the territory, penetrates into forest (“Altai and Western Siberia”) and meadow-steppe (“Steppes of Ukraine”) cultural phytocoenoses, where it forms spontaneous coenopopulations. The conditions of habitats and sizes of coenopopulations of I. thalictroides differ in the areas of NBG: the largest is in the area “Rare plants of flora of Ukraine” (2.6 × 2.6 m), medium in “Altai and Western Siberia” (sizes of loci 1.3 × 1.3 m, 0.4 × 0.4 m and 0.1 × 0.1 m), the smallest in “Steppes of Ukraine” (0.3 × 0.3 m). Rhythms of growth and development of I. thalictroides in the conditions of Kyiv quite correspond to seasonal fluctuations of temperatures. The earliest flowering of I. thalictroides was observed in the areas of the NBG in the first half of April 2020, the latest in the second half of April in 2021–2022; flowering period in the beginning of fruiting in all areas coincided. Coenotic conditions of I. thalictroides habitats in the composition of artificially formed vegetation in the NBG and in nature differ. In the NBG areas, the species grows together with introduced, native and alien plant species and shows significant ecological and coenotic plasticity. The age spectra of all coenopopulations of I. thalictroides on the NBG areas are left-handed. The total percentage of shoots of the pregenerative period of ontogenesis (juvenile, immature, virginіle) is very high (88.98–93.62 %). The highest values are for virginile shoots (71.78–82.98 %), which may be due to the predominance of vegetative propagation over seed. The spatial distribution of shoots in coenopopulations of I. thalictroides is group, which may be due to a combination of barochoria and vegetative propagation. The number of shoots in coenopopulations of I. thalictroides in different parts of the NBG differs significantly: the largest is in the area “Rare plants of flora of Ukraine” (3562 shoots), medium in “Altai and Western Siberia” (1017 shoots), the smallest in “Steppes of Ukraine” (47 shoots). In contrast, the average density of shoots in all coenopopulations of I. thalictroides is high, uniform and is 522–546 different age shoots per 1 m2. The dependence of the number of flowers and/or fruits оn generative shoots from the height of the shoots was not detected. A study of the gr","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123672284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of climatic factors in seasonal differences of CO2 emission from the soil and litter of broadleaf forest phytocoenoses","authors":"I. Vyshenska","doi":"10.18523/2617-4529.2022.5.39-43","DOIUrl":"https://doi.org/10.18523/2617-4529.2022.5.39-43","url":null,"abstract":"The article presents the results of the study of the intensity of carbon dioxide emission from the forest bedding and soil in the broad-leaved phytocoenosis of the National Nature Park “Holosiivskyi” (Kyiv green belt) in the spring and autumn periods of the year. The research was conducted in the autumn period in October, and in the spring period in April. Site coordinates: 50°17′52.7″N, 30°32′34.4″E. The experimental plot (50 × 50 m) was represented by the association Convallario majali-Quercetum roboris Shevchyk & V. Sl. in Shevchyk & al. 96, union Convallario majali-Quercion roboris Shevchyk, V. Sl. 96. It has been established that more intensive emission of CO2 occurs in the spring period compared to the autumn period. In the spring period of the year, the intensity of carbon dioxide emission from the forest litter and soil in the broad-leaved phytocoenosis ranged from 56.12 to 99.27 μmol CO2·m-2·min-1 and averaged 74.51 μmol of CO2·m-2·min-1. These differences depended on the amount of organic matter in the soil, the density of soil composition, the presence and size of tree roots, as well as the thickness of the forest bedding at the measurement points. In the autumn period of the year, the intensity of carbon dioxide emission from the forest bedding and soil was lower and in average was 68.52 μmol CO2·m-2·min-1. It was found out that higher values of carbon dioxide emissions in the spring period were determined by differences in temperature and humidity. Important factors were the increase in the supply of organic substances in the soil and more intensive processes of decomposition of these substances in the spring. Correlational analysis of the influence of climatic factors on the intensity of carbon dioxide emission proved the predominant role of temperature (r = 0.45) and a smaller influence of humidity (r = 0.15) of air and soil. In general, the conducted research confirmed the importance of monitoring the CO2 emission index of the forest bedding and soil respiration as indicators of the stability of forest ecosystems in the face of climate change.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130238432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of introgression hybridization for extension of genetic variability in recipient genome","authors":"M. Antonyuk, V. Shpylchyn, V. Martynenko, T. Ternovska","doi":"10.18523/2617-4529.2022.5.3-13","DOIUrl":"https://doi.org/10.18523/2617-4529.2022.5.3-13","url":null,"abstract":"Alien chromatin (introgressions) can be incorporated into the wheat genome either through recombination or through translocation. Lines of introgressive origin may not contain identified alien chromatin, although they appear to be changed relative to the original wheat sample by some characteristics that are registered at different levels of phenotype assessment. It has been shown numerous facts of the variability of the recipient genome of hybrid origin. They can be caused by irregular behavior of chromosomes in mitoses of somatic cells with hybrid genome, and DNA changes. Wide phenotypic variability was observed among the progeny of hybrid genomes up to appearance of phenotypic novelties. The study of DNA profiles using several methods revealed the presence of numerous genetic and epigenetic changes, including deletions, changes in gene expression, changes in the methylation pattern, and activation of retrotransposons in the recipient genome. When these changes were not directly related to alien fragments, they are considered as possible mechanisms of extension of genetic variability intra genome of hybrid origin. Transcriptomic analysis of lines of hybrid origin and parental plants using the RNA-seq analyses showed that the change of expression affected a different number of genes. The change in the expression of alien genes occurred in the direction of down-regulation, upregulation was typical for wheat genes. Phenotypic variability during introgressive hybridization can be accompanied by attenuation of the expression of an alien gene when it enters the recipient genome. That may be a consequence of the interaction between the alien gene and the genetic environment of the host genome. Today there is a lot of evidence that these mechanisms are epigenetic, which include DNA methylation, histone modification, the involvement of small RNAs in the regulation of gene expression, and the involvement of transposons. Moreover, none of these mechanisms act independently, but are a component of events, the results of which can be a change in gene expression and the appearance of a new phenotype that is not related to the expression of an alien gene.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130860407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ability of bone marrow progenitor cells to form colonies in ex vivo culture of MDS patients","authors":"D. Bilko, Margaryta Pakharenko, N. Bilko","doi":"10.18523/2617-4529.2021.4.21-25","DOIUrl":"https://doi.org/10.18523/2617-4529.2021.4.21-25","url":null,"abstract":"The results of in vitro hematopoiesis studies have provided most of the knowledge about the organization, regulation, and development of the human hematopoietic system over the past three to four decades. However, due to the impossibility of an appropriate assessment of hematopoietic stem cells (HSC) in humans and because of the shortcomings of methodological approaches to determining the role of hematopoietic progenitor cells in the pathogenesis of MDS and to predicting the course of the pathological process, semiliquid agar cultures of bone marrow from patients with myelodysplastic syndrome were used. Myelodysplastic syndrome (MDS) refers to a clinically, morphologically, and genetically heterogeneous group of diseases characterized by clonalism and arising from mutations at the level of hematopoietic progenitor cells. Proliferation of such a mutated stem cell progenitors leads to ineffective maturation of myeloid lineage cells and dysplastic changes in the bone marrow (BM). The aim of the study was to establish the relationship between the functional activity of hematopoietic progenitor cells in the ex vivo culture and the activity of the pathological process in the myelodysplastic syndrome. We studied bone marrow samples from patients with the myelodysplastic syndrome, namely refractory anemia with excess blasts I (MDS RAEB I) and refractory anemia with excess blasts II (MDS RAEB II) and AML under conditions in vitro, as well as their clinical laboratory data. It was found that the percentage of blasts and myeloblasts in the samples of patients with AML and MDS RAEB II increased, compared to the samples of patients with MDS RAEB I (63.5±3.9 %, 18.05±1.01 % and 9.49±1.53 % respectively). An increase in the number of erythrocytes and hemoglobin content was noted in the group of patients with MDS RAEB I compared with MDS RAEB II (2.9±1.4×1012 / l and 105.04±3.6 g / l versus 9±0.8×1012 / l and 84.5±4.8 g / l, respectively). The analysis of the results of BM studies of patients with MDS in in vitro culture indicated a significant lag in the formation of cell aggregates during cultivation and a pronounced inhibition of the colony-forming ability of progenitor cells, compared to the control. A noticeable decrease in the colony-forming ability was observed in patients with MDS RAEB I, MDS RAEB II and AML in this sequence – 4.1±1.2 per 1×105 explanted cells, 3.2±0.9 per 1×105 explanted cells and 2.0±0.6 per 1×105 explanted cells, respectively. The analysis of hematological parameters and the results of BM cells cultivation at different stages of MDS indicates that the colony-forming ability of progenitor cells correlates with the depth of the pathological process.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116623278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological research of exophytic tumors of the bronchi and the growth pattern of lung cancer","authors":"Lidiya Bolgova, T. Tuganova, A. Ponomarenko","doi":"10.18523/2617-4529.2021.4.26-31","DOIUrl":"https://doi.org/10.18523/2617-4529.2021.4.26-31","url":null,"abstract":"Background. The study of the growth of lung cancer (LC) has an important clinical significance for morphological verification, the choice of the treatment method, and the determining of prognosis. Investigation of this question allows to clarify the histogenesis of LC. The aim of our study was to compare the results of cytological studies of the material obtained during flexible bronchoscopy (FBS) and scrapings from the operated tumors of the bronchi to clarify the nature of LC growth. Design. To study the growth of tumors in the bronchi in relation to the bronchial mucosa, the cytological examination of the material obtained by FBS and scrapings from the surface of the operated bronchial tumors of 31 patients has been performed. Results. In the preoperative period, in the material of FBS, tumor cells were found only in 1/3 of patients. To obtain the optimal material from exophytic tumors of the bronchus, scrapings were carried out from the entire surface surgical material of the same patients. Cytological preparations of the obtained scrapings confirmed the presence of cancer cells in more than 1/3 of patients. In the rest of the observations, scrapings from the tumor surface contained only cells of the cylindrical epithelium. Conclusions. The cytological investigations made it possible to state that in 2/3 scrapings from surfaces of exophytic tumors of the bronchus contained only cells of the cylindrical epithelium; therefore, the tumors grow under it.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"212 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114971676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sporogenesis in F1 hybrids from the crosses between common wheat and lines with introgressions from Amblyopyrum muticum","authors":"Viktoriia Plyhun, M. Antonyuk, T. Iefimenko, T. Ternovska","doi":"10.18523/2617-4529.2021.4.13-20","DOIUrl":"https://doi.org/10.18523/2617-4529.2021.4.13-20","url":null,"abstract":"Hybridization of wheat lines comprising fragments of alien genetic material (introgression) with common wheat cultivars is effective and widespread means of transferring alien genes into genomes of modern wheat cultivars, and remains the main method of expending genetic pool of common wheat using genes of wild relatives. Success of such transfer depends on the processes of sporo- and gametogenesis in F1 hybrids, therefore cytological assessment of this processes is obligatory. Stages of meiosis and microgametogenesis were studied on cytological preparations of spikes of F1 hybrids from reciprocal crosses of common wheat cultivars and wheat lines of introgression origin with alien genetic material from wheat wild relative Amblyopyrum muticum. Sporogenesis in F1 hybrids occurs with disorders in both male and female sexual areas. Instead of 21 closed bivalents chromosome configurations in maximal association of chromosomes in M1 PMC could contain up to 8 open bivalents, up to 12 univalents, including three- and quadrivalents. In A1 lagging chromatids were observed, and up to 5 micronuclei per cell were registered in tetrads. Quantitative characteristics of chromosome associations in M1 PMC did not differ for hybrids obtained using introgression lines as female (direct crossing) and male (reverse crossing) cross components. The difference between reciprocal crosses was detected only for the quantity of cells in tetrads with different quantity of micronuclei. F1 hybrids from direct crosses had smaller portion of cells without micronuclei, and more cells with 1-3 micronuclei compared to hybrids of reverse crosses.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131380571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of climatic factors on the energy potential of broadleaf phytocoenosis forest bedding","authors":"I. Vyshenska, Alina Ginzhaliuk","doi":"10.18523/2617-4529.2021.4.32-36","DOIUrl":"https://doi.org/10.18523/2617-4529.2021.4.32-36","url":null,"abstract":"The study of the energy potential of forest bedding in the deciduous forest areas of the “Lisnyky” Nature Reserve of the Holosiivsky National Nature Park revealed the nonlinear nature of its annual dynamics. The main sources of replenishment of the organic stock of this component of broadleaf forest phytocoenosis were the active development of herbaceous vegetation in spring and significant leaf rainfall in autumn. At the same time, significant changes in the processes of accumulation and decomposition of organic matter of forest bedding occurred under the influence of climatic factors such as temperature and precipitation. The experimental site was located in moderately moist oak forest ass. Convallario majali-Quercetum roboris Shevchyk & V. Sl. in Shevchyk & al. 96. The age of the old Quercus robur oak trees, which occupy the first tier of the forest, exceeds 100 years. The second tier is formed by other broadleaf species, Acer platanoides, A. campestre, A. tataricum, Carpinus betulus, Fraxinus excelsior and Tilia cordata, up to 30 years old. The energy reserve was calculated from the dry organic matter content. The obtained data were compared with  meteorological data of the corresponding month of the year and correlation coefficients were calculated. Correlation analysis confirmed the relationship between climatic factors and the energy potential of forest bedding. The inverse of the mean force (R = -0.515) shows the relationship between the energy potential of the forest bedding and the average monthly temperature and the high direct correlation between the energy potential and the amount of average monthly precipitation (R = 0.755). In general, forest bedding can be considered an important component of the ecosystem, which responds quickly to external factors and characterizes the state of the forest ecosystem, and its energy potential can be an effective indicator of forest ecosystem stability in climate change monitoring.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114055895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotopes mapping in the aspect of local econetworks formation on the example of the “Kreidova Flora” Nature Reserve","authors":"Yuliia Spinova","doi":"10.18523/2617-4529.2021.4.47-50","DOIUrl":"https://doi.org/10.18523/2617-4529.2021.4.47-50","url":null,"abstract":"The article presents the results of mapping the biotopes of the “Kreidova Flora” Nature Reserve, which is one of the key areas of the eco-network of the local level of the newly created Kramatorsk district. This district includes almost entirely “Sviati Hory” National Nature Park, an object of the Nature Reserve Fund of Ukraine (NRF) with the highest amount of estimated points for a comprehensive assessment of sozological value, which belongs to a group of the first class. Two NRF objects (including the “Kreidova Flora” Nature Reserve) were from the group of the second class: one from the group of the third: nine from the group of the fifth class: and one from a group of the sixth class. The total number of points of the studied NRF objects of the newly created district is 465, which is a significant indicator. The key areas of the local level eco-network are the NRF with adjacent natural areas, which together form clusters. The ecological corridors of the local level are the river network of the district and the network of forest belts. As buffer zones for river-type eco-corridors, it is proposed to consider a minimum 200-meter water protection zone in accordance with Art. 87 of the Water Code of Ukraine. Restoration areas of the local econetwork of Kramatorsk district should be all areas with disturbed natural landscape (abandoned quarries, heaps), as well as abandoned agricultural lands, where it is proposed to introduce revalidation measures. The obtained maps and databases of distribution of different types of biotopes on the territory of the reserve will be the basis for its management plan, and this technique can be used within other structural elements of ecological networks. According to the same principle, it is considered necessary to conduct research in the entire study area, as well as other areas of Donetsk region.","PeriodicalId":173296,"journal":{"name":"NaUKMA Research Papers. Biology and Ecology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130052694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}