Journal of the International Society of Antioxidants in Nutrition & Health最新文献_第10页

Total Polyphenol, Flavonoid Contents and Antioxidant Activity of Red Kohlrabi Sprouts 红甘蓝芽总多酚、类黄酮含量及抗氧化活性研究

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_v3i4_1350

Cho Jayong, Ji Okjoo

{"title":"Total Polyphenol, Flavonoid Contents and Antioxidant Activity of Red Kohlrabi Sprouts","authors":"Cho Jayong, Ji Okjoo","doi":"10.18143/JISANH_v3i4_1350","DOIUrl":"https://doi.org/10.18143/JISANH_v3i4_1350","url":null,"abstract":"This study was conducted to clarify the bioactive activities of red kohlrabi sprouts(Brassica oleracea L. var. gongylodes L.), which were produced using the smart farm and drum-type rotary facilities arranged in a row. We have harvested red kohlrabi sprout vegetables at 6 days after germination which were sprouted at 20℃. Roots were harvested at 90 days after seedling, which were fresh weight 752g/ea. Samples were freeze drying to analyse the bioactive components. We have also examined the bioactive activity of sprouts and roots of kohlrabi after extracting with methanol. Total phenol and flavonoid contents of red kohlrabi sprouts increased significantly more than two times than those of roots. Antioxidant enzyme activity of red kohlrabi sprouts such as SOD, CAT, APX and POD also increased remarkably compared with that of roots. DPPH radical scavenging activity and ABTS radical scavenging activity of red kohlrabi sprouts were higher than those of roots. However, there were lower anti-cancer activities of sprouts against Calu-6 and MCF-7 cell lines than those of roots. Key-words : Kohlrabi, sprout, polyphenol, flavonoid, antioxidation, bioactive activity This study was conducted under the supply of \"Korea Small and Medium Business Administration(C0353901)\".","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85030011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulation of VEGF-A splicing using natural compounds as a potential therapeutic in diabetic nephropathy 使用天然化合物操纵VEGF-A剪接作为糖尿病肾病的潜在治疗方法

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1313

M. Stevens, S. Oltean, S. Harper

{"title":"Manipulation of VEGF-A splicing using natural compounds as a potential therapeutic in diabetic nephropathy","authors":"M. Stevens, S. Oltean, S. Harper","doi":"10.18143/JISANH_V3I4_1313","DOIUrl":"https://doi.org/10.18143/JISANH_V3I4_1313","url":null,"abstract":"Alternative splicing of vascular endothelial growth factor (VEGF-A) gives rise to isoform families, the pro-angiogenic VEGF-Axxx and the anti-angiogenic VEGF-Axxxb. VEGF-A165b has previously been shown to be protective in several murine models of renal disease, as well as providing cytoprotection to podocytes in culture. This study aimed to investigate whether a blueberry extract that promotes VEGF-A165b expression, is a potential therapeutic in a mouse model of type I diabetic nephropathy. The natural extract had no effect on blood glucose levels, but did delay the onset/progression of albuminuria in diabetic mice, and prevented increases in glomerular water permeability. Diabetic control mice had a reduced glomerular expression of nephrin and reduced glomerular capillary circumference, which were rescued when treated with the natural extract. HUVECs displayed reduced tube formation when treated with the natural extract, which was rescued upon addition of Ab-VEGF-A165b. Certain unknown compounds within the blueberry extract are able to switch VEGF-A splicing to promote VEGF-A165b expression in podocytes. The extract plays a protective role in the prevention and treatment of diabetic nephropathy in the DBA/2J type I diabetic mouse model. This study highlights the therapeutic potential of switching VEGF-A splicing to promote VEGF-A165b in diabetic nephropathy.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"52 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91494083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fortification of orange juice with antioxidant from orange by-products and evaluation of stability during in vitro digestion 从橙子副产品中提取抗氧化剂强化橙汁及其体外消化稳定性评价

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1320

Jorge G. Figueroa, Xavier Celi-Bonilla, Jessica Delgado-Sánchez, Soledad González-Arévalo, I. Borrás-Linares, A. Segura‐Carretero

{"title":"Fortification of orange juice with antioxidant from orange by-products and evaluation of stability during in vitro digestion","authors":"Jorge G. Figueroa, Xavier Celi-Bonilla, Jessica Delgado-Sánchez, Soledad González-Arévalo, I. Borrás-Linares, A. Segura‐Carretero","doi":"10.18143/JISANH_V3I4_1320","DOIUrl":"https://doi.org/10.18143/JISANH_V3I4_1320","url":null,"abstract":"Orange juice production provides large amounts of underutilised by-products that suppose an environmental problem for the food industry, despite their high content in bioactive compounds. The aims of this work have been the fortification of commercial orange juice with bioactive compounds from orange pulp in order to improve its antioxidant activity. Thus, different aqueous orange pulp extracts were encapsulated and incorporated into commercial orange juice at different proportions. The stability of the antioxidant ingredient was followed over 8 weeks. The total phenolic content and the antioxidant capacity of these formulations were measured with different antioxidant procedures. Additionally, the effect of an in-vitro digestion in the antioxidant activity of the fortified juices was studied. The total phenolic content of the developed ingredient decreased in 34 % during the two first weeks of storage, but after that it remain constant. The results revealed that the commercial orange juice fortified with the encapsulated extract from orange pulp showed an increase of total phenolic content of 10-22 %, while the antioxidant activity of the juice improved in all the antioxidant assays. The addition of the antioxidant extract showed a reduction in the decrease of the antioxidant activity of the juices after de in-vitro digestion.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88271219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiome species richness differ after intake of green tea and cranberry extracts by lean and obese mice 瘦小鼠和肥胖小鼠摄取绿茶和蔓越莓提取物后，肠道微生物种类丰富度不同

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1355

E. Anzenbacherová, J. Nekvindová, L. Jourová, P. Anzenbacher

{"title":"Gut microbiome species richness differ after intake of green tea and cranberry extracts by lean and obese mice","authors":"E. Anzenbacherová, J. Nekvindová, L. Jourová, P. Anzenbacher","doi":"10.18143/JISANH_V3I4_1355","DOIUrl":"https://doi.org/10.18143/JISANH_V3I4_1355","url":null,"abstract":"Green tea and cranberry are rich with phenolic compounds and they are extensively studied for their cardioprotective, anti-carcinogenic and anti-infective activities. This study analyzes their effect on gut microbiota and selected biochemical parameters in lean NMRI mice and in monosodium glutamate (MSG) – induced mouse model of obesity with insulin resistance and hyperinsulinemia. Results on the species richness show similarity of the groups that were fed by standard chow and the diet enriched with green tea extract, both in the normal (lean) mice and those with MSG-induced obesity. On the contrary, mice treated with cranberry extract tend to form a separate cluster of data points, showing a more pronounced difference between this group and control and green tea-treated groups. Again, the shift is present in both normal and obese group of mice. In other words, the analysis of species richness indicate a lowering of the number of taxonomic units after intake of cranberry extract; this effect was even more apparent in obese mice. On the contrary, the green tea extract did not cause a prominent change of the species richness of the mice gut microbiome. Supported by GACR303/12/G163.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89941933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenols bioavailability in piglets fed with grape pomace diet 葡萄渣饲粮中多酚类物质的生物利用度

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1357

V. Chedea, R. Pelmuș, M. Rotar, M. Gras, M. Hăbeanu, I. Țăranu

{"title":"Polyphenols bioavailability in piglets fed with grape pomace diet","authors":"V. Chedea, R. Pelmuș, M. Rotar, M. Gras, M. Hăbeanu, I. Țăranu","doi":"10.18143/JISANH_V3I4_1357","DOIUrl":"https://doi.org/10.18143/JISANH_V3I4_1357","url":null,"abstract":"The aim of the present study was to evaluate the influence of a diet containing 5% grape pomace (GP) on polyphenols absorption and their antioxidant antiradicalic activity (DPPH test) on weaning piglets. 20 crossbred TOPIG starter piglets were assigned to one of the 2 treatments: control (10 piglets- normal diet for weaned pig-C) and diet with GP (10 piglets -diet -D) for 36 days. After 15 days and at the end of the experimental period, blood samples were collected from the two groups. All piglets were slaughtered and the organs, liver, spleen, duodenum, colon, kidney, Longissimus dorsi muscle, mesenteric lymph nodes, heart and brain were collected. The total polyphenols (TP) content and the antioxidant activity of the feed diet and diet components as well as of the blood and organs were determined by using Folin Ciocalteu and DPPH test respectively. GP increased the TP content in feed as well as its antioxidant activity. Expressed as % of control, TP were found to be higher in plasma, duodenum, colon, kidneys and heart than in the control. The highest antioxidant activity was found in liver, kidneys and heart.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88428909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic polymorphisms of CYP enzymes and its influence on drug interactions between isoflavonoid genistein and model drugs CYP酶的遗传多态性及其对异黄酮染料木素与模型药物相互作用的影响

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_v3i4_1351

P. Anzenbacher, M. Zapletalová, Kristýna Krasulová, E. Anzenbacherová

{"title":"Genetic polymorphisms of CYP enzymes and its influence on drug interactions between isoflavonoid genistein and model drugs","authors":"P. Anzenbacher, M. Zapletalová, Kristýna Krasulová, E. Anzenbacherová","doi":"10.18143/JISANH_v3i4_1351","DOIUrl":"https://doi.org/10.18143/JISANH_v3i4_1351","url":null,"abstract":"Genetic polymorphism in cytochrome P450 (CYP) family of enzymes is a rather frequent cause of serious problems with proper dosing of drugs. This effect may be augmented by drug interaction between concomitantly taken drugs which are metabolized by the same enzymes. The second interacting partner however may easily be a compound of a natural origin as it is in the case of flavonoids from the grapefruit juice. We examined the effect of a prototypic isoflavonoid, genistein, on metabolism of probe drug substrates in genotypized human liver microsomes corresponding to slow, extensive or intermediate metabolizers with respect to CYP. The results document the most prominent effect of genistein and CYP2C19*2 allele on activity of this enzyme in slow metabolizers (down to aprox. 10%); the least effect was observed with CYP2D6*4 and CYP2D6*41 alleles (to about 60% of control value). In other words, inhibition of CYP activities by isoflavonoid (genistein) may be easily augmented by presence of defective alleles in patients of phenotypes of slow or intermediate metabolizers. Supported by a GACR P303/12/G163 Center of Excellence Project and by a Palacky University student´s project IGA_LF_2016_006.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81602031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semi-preparative HPLC isolation of bioactive compounds from Lippia citriodora extract: a useful nutraceutical approach 半制备高效液相色谱法分离柠檬黄提取物中的生物活性化合物:一种实用的营养保健品方法

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1359

M. L. Cádiz-Gurrea, María Elena Alañón-Pardo, D. Arráez-Román, S. Fernández-Arroyo, A. Segura‐Carretero

{"title":"Semi-preparative HPLC isolation of bioactive compounds from Lippia citriodora extract: a useful nutraceutical approach","authors":"M. L. Cádiz-Gurrea, María Elena Alañón-Pardo, D. Arráez-Román, S. Fernández-Arroyo, A. Segura‐Carretero","doi":"10.18143/JISANH_V3I4_1359","DOIUrl":"https://doi.org/10.18143/JISANH_V3I4_1359","url":null,"abstract":"Lippia citriodora (lemon verbena), a shrub indigenous to South America, was introduced into Europe at the end of the 17th century and has been widely used in infusions for its antispasmodic, antipyretic, sedative, and digestive properties. Furthermore, this plant is used in the food industry to flavour different products. Lemon verbena infusion contains significant amounts of polyphenols with healthy properties including high antioxidant activity1. Nevertheless, a better knowledge of the phenolic composition from lemon verbena polyphenols could be useful for nutraceutical development. In this work, the compounds in the L. citriodora extract were isolated by semipreparative HPLC according to their elution order. A total of 11 fractions were collected and the solvent was evaporated under vacuum. The residue of each fraction was weighted and dissolved with an appropriate volume of water at concentration level of 100 g/mL. Finally, fractions were filtered through a 0.25 μm filter. Afterwards, the composition of each fraction was established by a detailed HPLC-ESI/DAD-TOF-MS analysis2. A total of 30 compounds were identified in the L. citriodora extract belonging to various structural classes such as iridoids, flavonoids and phenylethanoids, most of them with a demonstrated antoxidant, anti-inflammatory and anticancer activities.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85965845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of flavonoids on cytochrome P450 activity in the gut – an in vitro food-drug interaction study 黄酮类化合物对肠道细胞色素P450活性的影响——体外食药相互作用研究

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1325

O. Farkas, D. Kovács, Z. Karancsi, G. Csikó, O. Palócz

{"title":"Effect of flavonoids on cytochrome P450 activity in the gut – an in vitro food-drug interaction study","authors":"O. Farkas, D. Kovács, Z. Karancsi, G. Csikó, O. Palócz","doi":"10.18143/JISANH_V3I4_1325","DOIUrl":"https://doi.org/10.18143/JISANH_V3I4_1325","url":null,"abstract":"Cytochrome P450 enzymes have a remarkable role in the xenobiotics metabolism. Oxidation of drugs and food components catalysed by the same isoenzyme can lead to drug-food interactions. Greatest quantity of CYP enzymes can be found in the liver. Nevertheless, the intestinal CYP garniture also contributes to the metabolism of clinically important drugs. Our research focused on the effects of flavonoids on the intestinal CYP enzymes. Intestinal epithelial cells (IPEC-J2) were treated by apigenin and apigenin-trimethylether. Phenobarbital was used as CYP inducer, mixture of naphtoflavone and ketoconazole as inhibitor. The CYP1A1, CYP1A2 and CYP3A activities were measured by chemiluminescent assay. Treatment of enterocytes with antipyrine was also performed in order to test possible drug interactions. Both flavones worked as significant CYP3A4 inhibitors. There was no significant difference between the inhibitory effects of the flavones at the same dose. However, apigenin-trimethylether combined with the inducer, was a more potent inhibitor, than the apigenin. Antipyrine decreased the enzymatic activity, which effect was enhanced, when antipyrine and apigenin were administered simultaneously. In conclusion, our results suggest that both apigenin and its trimethylated derivative can inhibit the intestinal CYP3A, which is the most important role in the metabolism of clinically relevant drugs.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83934823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of the effect of resveratrol isolated and in combination with tamoxifen on viability of breast cancer cells. 白藜芦醇分离及联合他莫昔芬对乳腺癌细胞活力的影响。

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1356

Beatriz Tinoco Franceschi, M. R. Torqueti

引用次数: 0

MODULATION OF IMMUNE AND ANTIOXIDANT RESPONSE IN WEANED PIGLETS BY BIOACTIVE COMPOUNDS FROM GRAPE SEED BY-PRODUCT 葡萄籽副产品生物活性化合物对断奶仔猪免疫和抗氧化反应的调节作用

Journal of the International Society of Antioxidants in Nutrition & Health Pub Date : 2016-06-03 DOI: 10.18143/JISANH_v3i4_1354

I. Țăranu, I. Grosu, A. Gras, M. Palade, V. Chedea, D. Marin

{"title":"MODULATION OF IMMUNE AND ANTIOXIDANT RESPONSE IN WEANED PIGLETS BY BIOACTIVE COMPOUNDS FROM GRAPE SEED BY-PRODUCT","authors":"I. Țăranu, I. Grosu, A. Gras, M. Palade, V. Chedea, D. Marin","doi":"10.18143/JISANH_v3i4_1354","DOIUrl":"https://doi.org/10.18143/JISANH_v3i4_1354","url":null,"abstract":"Grape processing for the production of wine generates a wide range of residues (pomace and seeds) rich in bioactive compounds (polyphenols, etc). Their biological potential has been already valorised in ruminant nutrition, but has been little investigated in pigs. This study was conducted to analyse the effect of dietary inclusion of grape seed by-product on growth performance, immune and antioxidant function in weaned pigs. A total of 12 weaned pigs were fed with a control or 5% grape seed cakes (GS) diets for 30 days. Pigs were sacrificed after 30 days, blood and organs were collected and stored at –80°C until analyses. The results showed that dietary GS did not exert significant effect on pig performance, but generated an anti-inflammatory effect by decreasing gene expression of TNF-α, IL-6, IFN-γ and other inflammatory molecules in liver. No effect on the activity and gene expression of antioxidant enzymes and malondialdehyde was also observed in liver. The effect on inflammation was less evident in spleen, but a slight increase in antioxidant enzyme activity was noticed. In conclusion, the dietary inclusion of GS modulated differentially immune and antioxidant response in weaned pig.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83657326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0