Journal of the autonomic nervous system最新文献

{"title":"Nitric oxide interacts with oxygen free radicals to evoke the release of adenosine and adenine nucleotides from rat hippocampal slices","authors":"R.M Broad,&nbsp;N Fallahi,&nbsp;B.B Fredholm","doi":"10.1016/S0165-1838(00)00124-7","DOIUrl":"10.1016/S0165-1838(00)00124-7","url":null,"abstract":"<div><p>The present study examined some possible mechanisms underlying the previously demonstrated release of adenosine by nitric oxide (NO) donors. Perfusion with the NO-donor <em>S</em>-nitroso-<em>N</em>-acetyl penicillamine (SNAP; 300 μM) led to a significant increase in the release of [³H]purines from both unstimulated and electrically stimulated hippocampal slices prelabeled with [³H]adenine. The NO-donor also evoked the release of endogenous ATP and ADP from unstimulated slices and, when combined with electrical stimulation, the release of ATP, AMP and adenosine. The SNAP-induced [³H]purine release was calcium-dependent, but not affected by the glutamate receptor antagonists MK-801 ((+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10-imine;100 nM) and CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 10 μM). Zaprinast (5 μM), an inhibitor of the cyclic GMP-dependent phosphodiesterase and 8-Br-cyclic GMP (0.01–1 mM) failed to evoke the release of purines, whereas generation of oxygen free radicals by xanthine plus xanthine oxidase did evoke purine release. Coperfusion of SNAP with the free radical scavengers superoxide dismutase (SOD; 60 μg/ml) and catalase (50 μg/ml) reduced or eliminated the ability of the NO-donor to enhance [³H]purine release, but the poly (ADP-ribosyl) synthetase (PARS) inhibitor benzamide (500 μM) did not affect it. These data indicate that NO interacts with superoxide, likely forming peroxynitrite, which subsequently acts to release adenosine and adenine nucleotides from hippocampal tissue.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 82-86"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00124-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonists and the purinergic nerve hypothesis: 2,2′-pyridylisatogen tosylate (PIT), an allosteric modulator of P2Y receptors. A retrospective on a quarter century of progress","authors":"M Spedding ,&nbsp;K Menton ,&nbsp;A Markham ,&nbsp;D.F Weetman","doi":"10.1016/S0165-1838(00)00142-9","DOIUrl":"10.1016/S0165-1838(00)00142-9","url":null,"abstract":"<div><p>2,2′-Pyridylisatogen tosylate (PIT) is a selective antagonist of P2Y responses in smooth muscle and does not antagonise the effects of adenosine. Responses to purinergic nerve stimulation are resistant to PIT. PIT is an allosteric modulator of responses to ATP in recombinant P2Y₁ receptors expressed in <em>Xenopus</em> oocytes with potentiation of ATP at low concentrations (0.1–10 μM) and antagonism at higher ones (&gt;10 μM). A radioligand binding profile showed that PIT did not interact with any other receptors, with the exception of low affinity for the adenosine A₁ receptor (p<em>K</em>_i, 5.3). The compound recognises purine sites and then may cause irreversible binding to sulfhydryl groups following prolonged incubation or high concentrations. PIT is a potent spin trapper.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 225-227"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00142-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21713039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Index","authors":"","doi":"10.1016/S0165-1838(00)00160-0","DOIUrl":"https://doi.org/10.1016/S0165-1838(00)00160-0","url":null,"abstract":"","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 295-296"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00160-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136987544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of fast synaptic transmission by presynaptic ligand-gated cation channels","authors":"Baljit S Khakh ,&nbsp;Graeme Henderson","doi":"10.1016/S0165-1838(00)00111-9","DOIUrl":"10.1016/S0165-1838(00)00111-9","url":null,"abstract":"<div><p>There is now considerable evidence demonstrating that ligand-gated cation channels (i.e., P2X, nicotinic, kainate, NMDA, AMPA and 5-HT₃ receptors), in addition to mediating fast excitatory neurotransmission, may be located presynaptically on nerve terminals in the peripheral and central nervous systems where they function to modulate neurotransmitter release. This modulation can be facilitation, inhibition or both. In this article, we first outline the multiple mechanisms by which activation of presynaptic ligand-gated cation channels can modulate spontaneous and evoked neurotransmitter release, before reviewing in detail published electrophysiological studies of presynaptic P2X, nicotinic, kainate, NMDA, AMPA and 5-HT₃ receptors.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 110-121"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00111-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relaxation of rat distal colon by activation of muscarinic, neuronal receptors: possible involvement of P2y-purinoceptors","authors":"Lars Börjesson,&nbsp;Arij Ali,&nbsp;Svante Nordgren,&nbsp;Dick S Delbro","doi":"10.1016/S0165-1838(00)00112-0","DOIUrl":"10.1016/S0165-1838(00)00112-0","url":null,"abstract":"<div><p>McN-A-343, which is a ligand at muscarinic receptors on myenteric ganglia, was found to concentration-dependently (1–44 μM) elicit non-adrenergic relaxation of the longitudinal muscle of rat distal colon, having been precontracted with carbachol (1 μM). This effect was partly antagonized by the muscarinic receptor antagonist, pirenzepine (0.3 μM), the nerve blocker, tetrodotoxin (1 μM), or by drugs which interfere with purinergic neurotransmission (apamin [0.5 μM], reactive blue 2 [50 μM]). Blockade of nitric oxide synthase (<span>l</span>-NNA [100 μM]), or of the cAMP (H-89 [1 μM]), or cGMP (ODQ [10 μM]) second messenger pathways did not affect the relaxatory response to McN-A-343 (14 μM). An additional, non-neurogenic component of the relaxation to this compound on carbachol induced tone is suggested to reflect a partial antagonism of the muscarinic receptors on the gut muscle by McN-A-343.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 53-58"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00112-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuronal morphology and the synaptic organisation of sympathetic ganglia","authors":"I.L. Gibbins,&nbsp;P. Jobling,&nbsp;J.P. Messenger,&nbsp;E.H. Teo,&nbsp;J.L. Morris","doi":"10.1016/S0165-1838(00)00132-6","DOIUrl":"10.1016/S0165-1838(00)00132-6","url":null,"abstract":"<div><p>In this article, we provide a short review of the structure and synaptic organisation of the final motor neurons in the sympathetic ganglia of mammals. Combinations of pathway tracing, multiple-labelling immunofluorescence and intracellular dye injection have shown that neurons in different functional pathways differ not only in their patterns of neuropeptide expression, but also in the size of their cell bodies and dendritic fields. Thus, vasoconstrictor neurons consistently are smaller than any other major functional class of neurons. Serial section ultrastructural analysis of dye filled neurons, together with electron microscopic and confocal microscopic analysis of immunolabelled synaptic inputs to sympathetic final motor neurons indicate that synapses are rare and randomly distributed over the surface of the neurons. The total number of synapses is simply proportional to the total surface area of the neurons. Many terminal boutons of peptide-containing preganglionic neurons do not make conventional synapses with target neurons. Furthermore, there is a spatial mismatch in the distribution of peptide-containing terminals and neurons expressing receptors for the corresponding peptides. Together, these results suggest that there are likely to be significant differences in the ways that the final sympathetic motor neurons in distinct functional pathways integrate their synaptic inputs. In at least some pathways, heterosynaptic actions of neuropeptides probably contribute to subtle modulation of ganglionic transmission.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 104-109"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00132-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of NGF, BDNF, bFGF, aFGF and cell density on NPY expression in cultured rat dorsal root ganglion neurones","authors":"Nóra Kerekes ,&nbsp;Marc Landry ,&nbsp;Karin Lundmark ,&nbsp;Tomas Hökfelt","doi":"10.1016/S0165-1838(00)00115-6","DOIUrl":"10.1016/S0165-1838(00)00115-6","url":null,"abstract":"<div><p>The effect of neurotrophic factors on neuropeptide Y (NPY) expression was studied in adult rat dispersed dorsal root ganglion (DRG) cultures. Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), acidic fibroblast growth factor (aFGF) or basic FGF was included in the culture medium during incubation for 72 h. In untreated cultures, around 18% of all neurones (visualized by antibodies to PGP 9.5) expressed NPY-like immunoreactivity (LI). In contrast, in vivo uninjured neurones do not contain detectable levels of NPY-LI. In the immunohistochemical analysis aFGF increased the percentage of NPY-immunoreactive (-IR) neurones 1.8-fold, while NGF, BDNF or bFGF had no significant effect on NPY expression. When the effect of these growth factors was monitored with non-radioactive in situ hybridization, both aFGF and bFGF caused a significant increase (2.25- and 1.8-fold, respectively), whereas, again, NGF and BDNF had no effect. The results also showed an effect of cell density on NPY expression, whereby fewer neurones expressed NPY in high than in low density cultures. This difference was seen in untreated as well as growth factor-treated cultures. The present results support the hypothesis that DRG neurones in culture are in an axotomized state, since they express NPY to about the same extent as axotomized DRG neurones in vivo. Surprisingly, two growth factors of the FGF family enhance NPY expression in DRG neurones, which is in apparent contrast to a published in vivo study [Ji, R.-R., Zhang, Q., Pettersson, R.F., Hökfelt, T., 1996. aFGF, bFGF and NGF differentially regulate neuropeptide expression in dorsal root ganglia after axotomy and induce autotomy. Reg. Pept. 66, 179–189.]. Finally, NPY expression was also influenced by cell density.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 128-138"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00115-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imagination and reality in the search for the P2Y receptors","authors":"Eric A Barnard","doi":"10.1016/S0165-1838(00)00147-8","DOIUrl":"10.1016/S0165-1838(00)00147-8","url":null,"abstract":"<div><p>A great body of evidence based on tissue and organ physiology and pharmacology led to the recognition, widespread by about 1990, that there must be cell membrane receptors for extracellular nucleotides to transduce their effects. This evidence was provided by the pioneering work of Geoffrey Burnstock and those who worked with him, or was developed by others starting from that information. This article will review how we could start from that foundation to clone the first known gene for such a receptor, P2Y₁. Some unusual properties of that receptor were revealed. I will consider further the P2Y receptors as a class — its definition, now that many such genes have become known. Imagination and reality have been intertwined in this saga.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 10-15"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00147-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATP as a peripheral mediator of pain","authors":"Sara G Hamilton,&nbsp;Stephen B McMahon","doi":"10.1016/S0165-1838(00)00137-5","DOIUrl":"10.1016/S0165-1838(00)00137-5","url":null,"abstract":"<div><p>This article reviews the extent to which recent studies substantiate the hypothesis that ATP functions as a peripheral pain mediator. The discovery of the P2X family of ion channels (for which ATP is a ligand) and, in particular, the highly selective distribution of the P2X₃<span><span> receptor within the rat nociceptive system has inspired a variety of approaches to elucidate the potential role of ATP as a pain mediator. ATP elicits excitatory inward currents in small diameter sensory ganglion cells. These currents resemble those elicited by ATP on recombinantly expressed </span>heteromeric P2X</span>_2/3 channels as well as homomultimers consisting of P2X₂ and P2X₃<span><span><span>. In vivo behavioural models have characterised the algogenic properties of ATP in normal conditions and in models of peripheral sensitisation. In humans, iontophoresis of ATP induces modest pain. In rats and humans the response is dependent on </span>capsaicin sensitive neurons and is augmented in the presence of </span>inflammatory mediators<span>. Since ATP can be released in the vicinity of peripheral nociceptive terminals under a variety of conditions, there exists a purinergic chain of biological processes linking tissue damage to pain perception. The challenge remains to prove a physiological role for endogenous ATP in activating this chain of events.</span></span></p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 187-194"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00137-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21712505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do sympathetic nerves release noradrenaline in “quanta”?","authors":"Lennart Stjärne","doi":"10.1016/S0165-1838(00)00119-3","DOIUrl":"10.1016/S0165-1838(00)00119-3","url":null,"abstract":"<div><p>The discovery of excitatory junction potentials (EJPs) in guinea-pig vas deferens by <span>Burnstock and Holman (1960)</span> showed for the first time that a sympathetic transmitter, now known to be ATP, is secreted in “quanta”. As it was assumed at the time that EJPS are triggered by noradrenaline, this discovery led to attempts to use the fractional overflow of noradrenaline from sympathetically innervated tissues to assess, indirectly, the number of noradrenaline molecules in the average “quantum”. The basic finding was that each pulse released 1/50 000 of the tissue content of noradrenaline, when reuptake was blocked and prejunctional α₂-adrenoceptors were intact. This provided the constraints, two extreme alternatives: (i) each pulse releases 0.2–3% of the content of a vesicle from all varicosities, or (ii) each pulse releases the whole content of a vesicle from 0.2 to 3% of the varicosities. New techniques have made it possible to address questions about the release probability in individual sites, or the “quantal” size, more directly. Results by optical (comparison of the labelling of SV2 and synaptotagmin, proteins in the membrane of transmitter vesicles), electrophysiological (excitatory junction currents, EJCs, at single visualized varicosities) and amperometric (the noradrenaline oxidation current at a carbon fibre electrode) methods reveal that transmitter exocytosis in varicosities is intermittent. The EJC and noradrenaline oxidation current responses (in rat arteries) to a train of single pulses were observed to be similar in intermittency and amplitude fluctuation. This suggests that they are caused by exocytosis of single or very few “quanta” of ATP and noradrenaline, respectively, equal to the contents of single vesicles, from a small population of release sites. These findings support, but do not conclusively prove the validity of the “intermittent” model of noradrenaline release. The question if noradrenaline is always secreted in packets of preset size (“quanta”) and if the “quantum” is a subfraction or the whole content of single synaptic vesicles, still remains open.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 236-243"},"PeriodicalIF":0.0,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00119-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21713041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}