Journal of the American Mosquito Control Association最新文献

A STAND-ALONE TOOL FOR MOSQUITO EGG ENUMERATION.

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-23 DOI: 10.2987/24-7184

Christopher C Evans, Andrew R Moorhead

{"title":"A STAND-ALONE TOOL FOR MOSQUITO EGG ENUMERATION.","authors":"Christopher C Evans, Andrew R Moorhead","doi":"10.2987/24-7184","DOIUrl":"https://doi.org/10.2987/24-7184","url":null,"abstract":"Accurate enumeration of mosquito eggs is crucial for various entomologic studies, including investigations into mosquito fecundity, life history traits, and vector control strategies. Traditional manual counting methods are labor intensive and prone to human error, highlighting the need for automated systems. This study presents a stand-alone automated mosquito egg counting system using a Raspberry Pi computer, high-quality camera, light-emitting diode ring light source, and a Python script leveraging the Open Source Computer Vision library. Linear regression analysis comparing automated and manual counts yielded a slope of 1.009 and an R2 value of 0.999, indicating a strong correlation between the methods. Bland-Altman analysis showed a bias of -0.5, with 95% limits of agreement ranging from -11.88 to 10.88. These results demonstrate the high accuracy and reliability of this system in laboratory settings. The automated system's portability, cost-effectiveness, and independence from an external computer make it particularly useful for diverse research environments. Variability in egg size and potential inaccuracies in field conditions with multiple mosquito species highlight areas for further refinement, and future work will focus on optimizing the counting algorithm and validating its performance across different mosquito species and rearing conditions to enhance its applicability in vector research.","PeriodicalId":17192,"journal":{"name":"Journal of the American Mosquito Control Association","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AERIAL BUFFER APPLICATIONS OF NALED FOR ADULT MOSQUITO CONTROL, ST. JOHNS COUNTY, NORTHEASTERN FLORIDA. 佛罗里达州东北部圣约翰县成蚊空中缓冲剂的应用。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-20 DOI: 10.2987/24-7190

Whitney A Qualls, Steven Smoleroff, Dana Smith, Rui-De Xue

{"title":"AERIAL BUFFER APPLICATIONS OF NALED FOR ADULT MOSQUITO CONTROL, ST. JOHNS COUNTY, NORTHEASTERN FLORIDA.","authors":"Whitney A Qualls, Steven Smoleroff, Dana Smith, Rui-De Xue","doi":"10.2987/24-7190","DOIUrl":"https://doi.org/10.2987/24-7190","url":null,"abstract":"Aerial applications of pesticides for adult mosquito control have emerged as effective tools in integrated mosquito management due to wider reach compared with ground-based methods. A key determinant for efficacy is the swath or line of spray deposition. This study aimed to assess the potential of aerial buffer sprays, which specifically target mosquito habitats, in comparison to traditional full-area aerial and ground-based applications. The efficacy and cost implications of these methods were evaluated in hot spot areas of St. Johns County, FL. Mosquito populations in 4 areas, 2 for buffer aerial (St. Johns County Golf Course and World Golf Village), 1 for full aerial (Flagler Estate), and 1 for ground-based (Elkton) treatments, were assessed using the Centers for Disease Control and Prevention light traps baited with octenol. Following treatments, mosquito population reductions and service request changes were evaluated. For aerial applications, the organophosphate naled was used, while ground applications used the pyrethroid Aqualuer® 20-20. Buffer aerial applications averaged 668.5 ha, significantly smaller than full aerial treatments (3750.2 ha). Cost and volume for buffer applications were notably lower ($1,998.47 and 34.1 liters) than full treatments ($10,558.40 and 180.6 liters). Mosquito populations posttreatment showed a 63.6% reduction for buffer applications, 59% for full aerial applications, and a 52% increase for ground-based treatments. Service request reductions were varied, with a slight increase (0.13%) after buffer aerial treatments and substantial reductions after full aerial (29.4%) and ground applications (87.5%). Buffer aerial applications demonstrated potential as an effective and cost-efficient method for mosquito control in targeted habitats. Although further evaluations are essential, such strategies could reshape mosquito management practices in regions similar to the Anastasia Mosquito Control District of St. Johns County.","PeriodicalId":17192,"journal":{"name":"Journal of the American Mosquito Control Association","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AEDES JAPONICUS: A TENANT INVADER IN THE GREAT SMOKY MOUNTAINS NATIONAL PARK, USA1. 日本伊蚊：美国大烟山国家公园的入侵者。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-16 DOI: 10.2987/24-7203

Mackenzie Campbell, Mitch Mullin, Roxanne Connelly, Paul E Super, Brian D Byrd

{"title":"AEDES JAPONICUS: A TENANT INVADER IN THE GREAT SMOKY MOUNTAINS NATIONAL PARK, USA1.","authors":"Mackenzie Campbell, Mitch Mullin, Roxanne Connelly, Paul E Super, Brian D Byrd","doi":"10.2987/24-7203","DOIUrl":"https://doi.org/10.2987/24-7203","url":null,"abstract":"Invasive organisms may cause ecologic, economic, and public health harm. Aedes japonicus is an invasive mosquito species of known ecologic and public health importance that has widely spread throughout the eastern USA since initially being recognized in Connecticut in 1998. Here, we report the known distributions of Ae. japonicus within the Great Smoky Mountains National Park (GSMNP) since its initial recognition in the park in 2004. From 2006 to 2022, we sampled eggs, larvae, and adult life stages through targeted, haphazard, and convenience collections. Through these efforts, we surveyed 23 (54.7%) of the 42 watersheds within the GSMNP. Aedes japonicus was present in 19 (82.6%) of the sampled watersheds, and the species was confirmed, in some instances, at the same location over multiple years, suggesting it remains entrenched. This species was observed in 45.2% of the GSMNP watersheds at elevations ranging from 347 to 1,478 m. Naturally occurring containers (i.e., riverine rock pools) were common collection sites in this study. The results of our findings are presented in the context of the species distribution within the park, the public health relevance given the GSMNP's public visitation rate (>12 million annually), potential species interactions, and the persistence of this species over the multiyear study.","PeriodicalId":17192,"journal":{"name":"Journal of the American Mosquito Control Association","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PHYLOGENETIC ANALYSIS OF AEDES AEGYPTI IN TAIF AND JEDDAH GOVERNORATES, SAUDI ARABIA. 沙特阿拉伯塔伊夫省和吉达省埃及伊蚊的系统发育分析。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-09 DOI: 10.2987/24-7194

Wafa Mohammed Al-Otaibi, Raed Hamoud M Algethami, Nahed Ahmed Hussien, Jazem A Mahyoub

{"title":"PHYLOGENETIC ANALYSIS OF AEDES AEGYPTI IN TAIF AND JEDDAH GOVERNORATES, SAUDI ARABIA.","authors":"Wafa Mohammed Al-Otaibi, Raed Hamoud M Algethami, Nahed Ahmed Hussien, Jazem A Mahyoub","doi":"10.2987/24-7194","DOIUrl":"https://doi.org/10.2987/24-7194","url":null,"abstract":"With their diverse species, mosquitoes are known to transmit the causal agents of diseases such as malaria, dengue, and yellow fever. Their high adaptability, attraction to humans, and variable adult behaviors make them a significant health concern. The focus on Aedes aegypti is significant for reducing vector-human contacts, monitoring insecticide resistance, and developing innovative vector management strategies. Given the scarcity of studies on Ae. aegypti in the western region of Saudi Arabia, this research is a significant step forward. The study aims to analyze the genetic variations and conduct a phylogenetic study of forty Ae. aegypti samples collected from Taif and Jeddah governorates of Saudi Arabia. The mitochondrial cytochrome c oxidase subunit I (COI) locus was targeted for genetic variance and phylogenetic analysis. Sequences of COI of Ae. aegypti isolates were submitted to the DNA Data Bank of Japan (DDBJ) and National Center for Biotechnology Information (NCBI) Genbank and compared with other global Aedes species isolates. The phylogenetic analysis shows that Ae. aegypti samples from Jeddah have identities ranging from 96.9% to 99.8%, closely related to the Peru (MN299016) and Cambodia (MN299014) isolates. Taif isolates have genetic similarities ranging from 97.5% to 99.8%, closely related to the Germany (KY022526) isolate. Sequence alignment and pairwise comparison show variation among the populations of Ae. aegypti from Taif and Jeddah regions (74.24-98.84%) with a genetic divergence distance of 0.008-0.12. In comparison, ranges slightly change with other Ae. aegypti (79.92-95.96%, 0.008-0.01) as well as Ae. albopictus populations (74.13-83.58%, 0.13-0.20) found in the Genbank database. According to our findings, the present study provides information for a local variation of Ae. aegypti in the western region of Saudi Arabia that could help in disease mapping and risk mitigation, thereby enhancing our ability to manage disease vectors effectively.","PeriodicalId":17192,"journal":{"name":"Journal of the American Mosquito Control Association","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NEW OCCURRENCES OF PHYTOTELMATA-INHABITING MOSQUITOES SPECIES IN NORTHWESTERN ARGENTINA. 阿根廷西北部植毛虱属蚊种新发。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-09 DOI: 10.2987/24-7205

Gabriela C Flores, Roberto D Coello Peralta, Renato León, Paul L Duque, Gustavo Molina, Jonathan Liria-Salazar, Raúl E Campos, María J Dantur Juri

引用次数: 0

Occurrence Of Limatus Durhamii In Artificial Containers In Atlantic Forest, São Paulo, Brazil. 巴西圣保罗大西洋森林人工容器中杜哈密狐的发生。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-09 DOI: 10.2987/24-7197

Karolina Morales Barrio-Nuevo, Antonio Ralph Medeiros-Sousa, Eduardo Evangelista, Walter Ceretti-Junior, Aristides Fernandes, Marcia Bicudo DE Paula, Mauro Toledo Marrelli, Rafael Oliveira-Christe

引用次数: 0

FIRST STATE AND NATIONAL RECORDS OF CULICOIDES DEBILIPALPIS AND CULICOIDES REEVESI IN CHIHUAHUA, MEXICO. 墨西哥奇瓦瓦州首次记录了德比利帕库蠓和里氏库蠓。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2025-01-02 DOI: 10.2987/24-7207

Rodolfo González-Peña, Stephanie V Laredo-Tiscareño, Herón Huerta, Luis M Hernández-Triana, Erick De Jesús De Luna-Santillana, Jaime R Adame-Gallegos, Carlos A Rodríguez-Alarcón, Julián E García-Rejón, David O Hidalgo-Martínez, Javier A Garza-Hernández

引用次数: 0

COMPARING DETECTIONS OF AEDES AEGYPTI FEMALES USING THREE TYPES OF AUTOCIDAL GRAVID TRAPS. 比较三种自杀式妊娠诱捕器对埃及伊蚊雌蚊的检测结果。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2024-12-23 DOI: 10.2987/24-7195

Marta I Diaz-Garcia, Victoria Ocasio-Reed, Gilberto Felix, Gabriela Paz-Bailey, Lee Ching Ng, Roberto Barrera

{"title":"COMPARING DETECTIONS OF AEDES AEGYPTI FEMALES USING THREE TYPES OF AUTOCIDAL GRAVID TRAPS.","authors":"Marta I Diaz-Garcia, Victoria Ocasio-Reed, Gilberto Felix, Gabriela Paz-Bailey, Lee Ching Ng, Roberto Barrera","doi":"10.2987/24-7195","DOIUrl":"https://doi.org/10.2987/24-7195","url":null,"abstract":"We compared the number of Aedes aegypti females per trap and the number of detections of this mosquito species per week during 8 wk in 3 types of autocidal gravid traps, the Centers for Disease Control and Prevention (CDC) Autocidal Gravid Ovitrap (AGO), Biogents Gravid Aedes Trap (GAT), and Singapore Gravitrap (SGT), in central Puerto Rico. These traps use the same principles for attracting gravid Ae. aegypti females as traditional ovitraps, such as dark colors, standing water, and decomposing plant materials. The traps differ in size, AGO being the biggest and SGT the smallest. Average captures of female Ae. aegypti per trap per week were 11.1, 7.2, and 1.7 in AGO, GAT, and SGT traps, respectively, a pattern consistent with the sizes of the traps. These results indicated that GAT traps and SGT traps captured 35.5% and 84.7% fewer females of Ae. aegypti, respectively, than AGO traps. Although Ae. aegypti was present in all 20 sites during the 8 wk of observations, AGO, GAT, and SGT traps did not catch specimens in 1, 9, and 58 out of 160 observations per trap type (trap-wk), respectively. Trap failures were 1, 6, and 1 for the AGO, GAT, and SGT traps, respectively. Despite the absence of females of Ae. aegypti at some sites and weeks in each of the traps, all 3 traps were able to detect the presence of this mosquito at each of the 20 sites during the 8 wk of observations and could be used for Ae. aegypti surveillance.","PeriodicalId":17192,"journal":{"name":"Journal of the American Mosquito Control Association","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPARISON OF NANOPORE AND CLASSICAL SANGER SEQUENCING TO IDENTIFY MOSQUITO BLOODMEAL HOSTS. 纳米孔测序与经典桑格测序鉴定蚊血宿主的比较。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2024-12-12 DOI: 10.2987/24-7191

Linda Kothera, Spencer J Hills, Nicholas A Panella, Aaron C Brault

{"title":"COMPARISON OF NANOPORE AND CLASSICAL SANGER SEQUENCING TO IDENTIFY MOSQUITO BLOODMEAL HOSTS.","authors":"Linda Kothera, Spencer J Hills, Nicholas A Panella, Aaron C Brault","doi":"10.2987/24-7191","DOIUrl":"https://doi.org/10.2987/24-7191","url":null,"abstract":"The tools available to vector control districts (VCDs) to collect mosquito surveillance data are constantly evolving. As more VCDs obtain real-time polymerase chain reaction (PCR) instruments and the costs associated with computing power and next-generation sequencing continue to decrease, the option of generating useful molecular data in-house becomes more viable. Measures such as arbovirus testing and genotyping for insecticide resistance mutations using RT-qPCR, and identifying species used for mosquito bloodmeals with next-generation sequencing or Sanger sequencing are examples. In this study we identify mosquito host bloodmeal species using Nanopore sequencing from Oxford Nanopore Technologies. We used MinION and Flongle flow cells and a Mk1C device to sequence 96 barcoded amplicon samples in a single sequencing run, and share details of data analysis using the free-to-use Galaxy bioinformatics platform. After sequencing the same samples with Sanger sequencing, we conclude that Nanopore sequencing is better at identifying species in mixed bloodmeals. This work demonstrates a potential use of nanopore sequencing by VCDs with basic biology laboratory and computing equipment.","PeriodicalId":17192,"journal":{"name":"Journal of the American Mosquito Control Association","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEASONAL MOSQUITO PRODUCTION IN STORM SEWER CATCH BASINS OF THE NORTH SHORE SUBURBS OF CHICAGO, 2021 TO 2024. 2021 年至 2024 年芝加哥北岸郊区雨水渠集水盆地的季节性蚊子产量。

IF 0.9 4区农林科学

Journal of the American Mosquito Control Association Pub Date : 2024-12-01 DOI: 10.2987/24-7202

Justin E Harbison

引用次数: 0