Journal of steroid biochemistry最新文献_第5页

Progestin binds to the glucocorticoid receptor and mediates antiglucocorticoid effect in rat adipose precursor cells 黄体酮在大鼠脂肪前体细胞中与糖皮质激素受体结合并介导抗糖皮质激素作用

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90089-B

Xuefan Xu , Johan Hoebeke , Per Björntorp

{"title":"Progestin binds to the glucocorticoid receptor and mediates antiglucocorticoid effect in rat adipose precursor cells","authors":"Xuefan Xu , Johan Hoebeke , Per Björntorp","doi":"10.1016/0022-4731(90)90089-B","DOIUrl":"10.1016/0022-4731(90)90089-B","url":null,"abstract":"<div>The binding of progestin and glucocorticoid hormones was examined in the cytosol of rat adipose precursor cells. Progestin binding sites of high affinity and limited capacity were present in the cytosol of adipose precursor cells from female rats, but not from male rats, by using [3H]R5020 as radioligand. Glucocorticoid binding sites of high affinity and limited capacity were present in the cytosol of these cells from both male and female rats by using [3H]dexamethasone and [3H]triamcinolone acetonide as radioligands. The dissociation constants were in the physiological concentration range. Studies of competitive binding showed that progestin could compete with glucocorticoids at glucocorticoid binding sites. In a serum free medium glucocorticoid effect on cellular differentiation, monitored by glycerophosphate dehydrogenase (GPDH), was effectively counteracted by progesterone which by itself had no effect.These results demonstrate that progestin receptor exists only in rat adipose precursor cells from female rats, while glucocorticoid receptor exists in rat adipose precursor cells of both sexes. Glucocorticoid effects on cellular differentiation in these cells are mediated by the glucocorticoid receptor. Progestin binds to the glucocorticoid receptor and antagonizes glucocorticoid effect on cellular differentiation in these cells.</div>","PeriodicalId":17138,"journal":{"name":"Journal of steroid biochemistry","volume":"36 5","pages":"Pages 465-471"},"PeriodicalIF":0.0,"publicationDate":"1990-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0022-4731(90)90089-B","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13367567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Influence of intestinal bacterial desulfation on the enterohepatic circulation of dehydroepiandrosterone sulfate 肠道细菌脱硫对硫酸脱氢表雄酮肠肝循环的影响

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90087-9

J. Van Eldere, J. Mertens, H. Eyssen

{"title":"Influence of intestinal bacterial desulfation on the enterohepatic circulation of dehydroepiandrosterone sulfate","authors":"J. Van Eldere, J. Mertens, H. Eyssen","doi":"10.1016/0022-4731(90)90087-9","DOIUrl":"10.1016/0022-4731(90)90087-9","url":null,"abstract":"<div>Selective association of germ-free (GF) rats with dehydroepiandrosterone sulfate (DHEAS) desulfating bacteria allowed us to assess the exact impact of intestinal bacterial desulfation on the excretion and enterohepatic circulation of orally administered DHEAS. Germ-free rats selectively associated with the DHEAS-desulfating strain Peptococcus niger H4 (H4 rats) excreted 50% of the total label recovered within 17 h vs 21 h in GF rats and 13 h 23 min in conventional (CV) rats. Germ-free rats excreted 30% of the total label recovered via their urine. However, association of GF rats with the desulfating microorganism increased urinary excretion to 46%, comparable to the 45.5% found in CV rats. Fractionation of fecal label yielded 70% sulfoconjugated DHEAS and 2% unconjugated dehydroepiandrosterone in GF rats vs 5 and 77% in CV rats, and 55 and 14% in H4 rats, respectively. Our results demonstrate that the intestinal bacterial desulfation of DHEAS stimulated the enterohepatic circulation of DHEAS. This in turn increased the urinary excretion of label resulting in an accelerated elimination of labeled DHEAS from the body.</div>","PeriodicalId":17138,"journal":{"name":"Journal of steroid biochemistry","volume":"36 5","pages":"Pages 451-456"},"PeriodicalIF":0.0,"publicationDate":"1990-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0022-4731(90)90087-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13299806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Oestrogen enhances the responsiveness of the MMTV-LTR to glucocorticoid in ZR-75-1 human breast cancer cells 雌激素增强ZR-75-1人乳腺癌细胞中MMTV-LTR对糖皮质激素的反应性

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90080-C

Gurpal S. Bansal, David S. Latchman

引用次数: 3

A novel lanosterol isomer produced in response to azole antifungals 一种新的羊毛甾醇异构体对唑类抗真菌药的反应

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90095-A

S.A. Howell, A.I. Mallet

引用次数: 2

Estrogen-like effects of 7,12-dimethylbenz(a)anthracene on the female rat hypothalamo-pituitary axis 7,12-二甲基苯(a)蒽对雌性大鼠下丘脑-垂体轴的雌激素样作用

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90092-7

Catherine Pasqualini , Alain Sarrieau , Monique Dussaillant , Maithé Corbani , Florence Bojda-Diolez , William Rostène , Bernard Kerdelhué

{"title":"Estrogen-like effects of 7,12-dimethylbenz(a)anthracene on the female rat hypothalamo-pituitary axis","authors":"Catherine Pasqualini , Alain Sarrieau , Monique Dussaillant , Maithé Corbani , Florence Bojda-Diolez , William Rostène , Bernard Kerdelhué","doi":"10.1016/0022-4731(90)90092-7","DOIUrl":"10.1016/0022-4731(90)90092-7","url":null,"abstract":"<div>We have recently demonstrated that 7,12-dimethylbenz(a)anthracene (DMBA), a potent inducer of mammary tumors in rodents, can in vitro decrease the number of membrane dopamine D2 receptors and stimulate prolactin (PRL) release, by direct estrogen-like actions on anterior pituitary. In the present study, we tested the ability of DMBA to mimic the in vivo estradiol (17βE2) effects on pituitary D2 receptors and on PRL as well as LH release. We have found that DMBA, like 17βE2, when injected to ovariectomized rats, induced a decrease in the number of anterior pituitary D2 receptors, a release of PRL and exerted a biphasic (acute negative and longer term positive) action on LH secretion. We thus examined the ability of DMBA to interact with 17βE2 receptors in the hypothalamo-pituitary axis: DMBA binds to the pituitary cytosolic estrogen receptors with an affinity 0.001% that of 17βE2. Finally [3H]DMBA binds to hypothalamus-containing brain sections. This binding was displaced partially by RU 2858 a pure estrogen agonist and totally by tamoxifen, a purported estrogen antagonist. No competition for [3H]DMBA binding was observed with an androgen (RU 1881) or a glucocorticoid (RU 26988) agonist.From these data, it may be concluded that DMBA can act as a partial estrogen in pituitary and hypothalamic tissues.</div>","PeriodicalId":17138,"journal":{"name":"Journal of steroid biochemistry","volume":"36 5","pages":"Pages 485-491"},"PeriodicalIF":0.0,"publicationDate":"1990-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0022-4731(90)90092-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13299808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Use of a monoclonal antibody to estrone-3-glucuronide in an enzyme-linked immunosorbent assay (ELISA) 雌激素-3-葡萄糖醛酸单克隆抗体在酶联免疫吸附试验(ELISA)中的应用

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90085-7

Peter A. Elder, Laurette Manley, John G. Lewis

引用次数: 11

Further characterization of the inhibitory effect of monensin on adrenal steroidogenesis 莫能菌素对肾上腺甾体生成抑制作用的进一步表征

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90093-8

Behling Cheng , Iris A. Horst , Jerome Kowal

{"title":"Further characterization of the inhibitory effect of monensin on adrenal steroidogenesis","authors":"Behling Cheng , Iris A. Horst , Jerome Kowal","doi":"10.1016/0022-4731(90)90093-8","DOIUrl":"10.1016/0022-4731(90)90093-8","url":null,"abstract":"<div>We have previously reported that treatment of cultured mouse adrenal tumor cells with 0.6-1.2 μ M monensin, a monovalent carboxylic ionophore, results in disruption of the organized structure of the Golgi complex. This is associated with an inhibition of adrenocorticotropic hormone (ACTH) or dibutyryl cAMP-stimulated steroidogenesis and impairment of mitochondrial cholesterol side-chain cleavage activity. The present report describes further investigations regarding possible mechanisms for the inhibition. Monensin inhibits both synthesis of fluorogenic steroids and incorporation of [14C]acetate into the end-product steroid 11β,20α-dihydroxy-4-pregnen-3-one. Supplementation of monensin-treated cells with 25-hydroxycholesterol, a readily available substrate for steroidogenesis, does not reverse the inhibitory effect on the reaction. The incorporation of l-[35S]methionine into trichloroacetic acid precipitable proteins in the isolated mitochondria of monensin-treated cells is inhibited approximately by 40%, whereas the inhibitory effect on the proteins in the cell homogenate is marginal. These findings suggest that a deficiency of newly synthesized proteins in mitochondria, rather than the availability of the substrate cholesterol, may be the primary factor causing impairment of steroidogenesis.</div>","PeriodicalId":17138,"journal":{"name":"Journal of steroid biochemistry","volume":"36 5","pages":"Pages 493-499"},"PeriodicalIF":0.0,"publicationDate":"1990-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0022-4731(90)90093-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13323758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Turner syndrome 特纳综合征

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90099-E

引用次数: 0

10th International symposium of the journal of steroid biochemistry and molecular biology 第10届类固醇生物化学与分子生物学国际研讨会

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90101-W

引用次数: 0

Short-term stimulatory effect of sertoli cell conditioned medium on leydig cell steroidogenesis is not mediated by inhibin 支持细胞条件培养基对间质细胞类固醇生成的短期刺激作用不是由抑制素介导的

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI: 10.1016/0022-4731(90)90086-8

A.J. Grootenhuis, R. Melsert, M.A. Timmerman, J.W. Hoogerbrugge, F.F.G. Rommerts, F.H. De Jong

引用次数: 10