Influence of intestinal bacterial desulfation on the enterohepatic circulation of dehydroepiandrosterone sulfate

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI:10.1016/0022-4731(90)90087-9

J. Van Eldere, J. Mertens, H. Eyssen

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引用次数: 5

Abstract

Selective association of germ-free (GF) rats with dehydroepiandrosterone sulfate (DHEAS) desulfating bacteria allowed us to assess the exact impact of intestinal bacterial desulfation on the excretion and enterohepatic circulation of orally administered DHEAS. Germ-free rats selectively associated with the DHEAS-desulfating strain Peptococcus niger H4 (H4 rats) excreted 50% of the total label recovered within 17 h vs 21 h in GF rats and 13 h 23 min in conventional (CV) rats. Germ-free rats excreted 30% of the total label recovered via their urine. However, association of GF rats with the desulfating microorganism increased urinary excretion to 46%, comparable to the 45.5% found in CV rats. Fractionation of fecal label yielded 70% sulfoconjugated DHEAS and 2% unconjugated dehydroepiandrosterone in GF rats vs 5 and 77% in CV rats, and 55 and 14% in H4 rats, respectively. Our results demonstrate that the intestinal bacterial desulfation of DHEAS stimulated the enterohepatic circulation of DHEAS. This in turn increased the urinary excretion of label resulting in an accelerated elimination of labeled DHEAS from the body.

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肠道细菌脱硫对硫酸脱氢表雄酮肠肝循环的影响

无菌(GF)大鼠与脱氢表雄酮硫酸脱氢表雄酮(DHEAS)脱硫细菌的选择性关联使我们能够评估肠道细菌脱硫对口服DHEAS排泄和肠肝循环的确切影响。与dheas脱硫菌株黑胃球菌H4选择性相关的无菌大鼠(H4大鼠)在17小时内排泄了总标签的50%，而GF大鼠为21小时，常规(CV)大鼠为13小时23分钟。无菌大鼠通过尿液排泄了总标签的30%。然而，GF大鼠与脱硫微生物的关联增加了46%的尿排泄，而CV大鼠的比例为45.5%。粪标分离在GF大鼠中产生70%的硫偶联DHEAS和2%的非偶联脱氢表雄酮，而CV大鼠分别为5%和77%，H4大鼠为55%和14%。我们的研究结果表明，肠道细菌对DHEAS的脱硫刺激了DHEAS的肠肝循环。这反过来又增加了尿液中标签的排泄，从而加速了从体内消除标签DHEAS。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of steroid biochemistry

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