Journal of submicroscopic cytology and pathology最新文献_第4页

Reduplicated basal lamina of the peritubular capillaries in renal biopsy specimens. 肾活检标本中小管周围毛细血管的重复基层。

Journal of submicroscopic cytology and pathology Pub Date : 2005-11-01

S Sato, H Kitamura, A Adachi, Y Sasaki, M Ishizaki, K Wakamatsu, K Inoue, Y Sugisaki, M Ghazizadeh

{"title":"Reduplicated basal lamina of the peritubular capillaries in renal biopsy specimens.","authors":"S Sato, H Kitamura, A Adachi, Y Sasaki, M Ishizaki, K Wakamatsu, K Inoue, Y Sugisaki, M Ghazizadeh","doi":"","DOIUrl":"","url":null,"abstract":"Reduplicated basal lamina of the peritubular capillaries (PTC) is usually found in kidney allografts in association with chronic transplant nephropathy and sometimes in native renal biopsies. In order to assess the incidence of this phenomenon in native renal biopsy specimens, we have carried out a retrospective review of the diagnostic ultrastructural pathology records of 80 consecutive renal biopsies excluding renal allografts and children with clinical signs of heavy proteinuria. Reduplicated basal lamina of the PTC was found in 19 out of the 80 cases (23.8%) with renal diseases. It was frequently seen in lupus nephritis, IgA nephropathy, and membranoproliferative glomerulonephropathy, being the subtypes of mesangial proliferative lesions. In a few cases it was also found in anti-neutrophil cytoplasmic autoantibody (ANCA) associated glomerulonephritis and benign nephrosclerosis renal biopsies. Reduplicated basal lamina of the PTC was strongly associated with glomerular and peritubular inflammation, and tubular necrosis. Peritubular interstitial edema, slight to moderately increased collagen fibrils, many spiraled collagen fibrils (indicative of degeneration), and collagen fibrils drawing from basal lamina were found around the reduplicated basal lamina of the PTC but not in normal basal lamina. These results indicate that in native renal biopsy specimens, reduplication of the basal lamina of the PTC is associated with endothelial cell injury and capillary permeability abnormality.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25971122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural investigations on protective effects of NCX 4016 (nitroaspirin) on macrovascular endothelium in diabetic Wistar rats. 硝酸阿司匹林ncx4016对糖尿病大鼠大血管内皮保护作用的超微结构研究。

Journal of submicroscopic cytology and pathology Pub Date : 2005-08-01

M V Ambrosini, G Mariucci, M G Rambotti, M Tantucci, C Covarelli, L De Angelis, P Del Soldato

引用次数: 0

Factors which influence sperm ability to fertilize. 影响精子受精能力的因素。

Journal of submicroscopic cytology and pathology Pub Date : 2005-08-01

F Geraci, G Giudice

引用次数: 0

Apoptosis of Trypanosoma musculi co-cultured with LPS activated macrophages: enhanced expression of nitric oxide synthase INF-gamma and caspase. 与LPS激活巨噬细胞共培养的肌肉锥虫的凋亡:一氧化氮合酶inf - γ和半胱天蛋白酶的表达增强。

Journal of submicroscopic cytology and pathology Pub Date : 2005-08-01

A Gugssa, S Gebru, C M Lee, B Baccetti, W Anderson

{"title":"Apoptosis of Trypanosoma musculi co-cultured with LPS activated macrophages: enhanced expression of nitric oxide synthase INF-gamma and caspase.","authors":"A Gugssa, S Gebru, C M Lee, B Baccetti, W Anderson","doi":"","DOIUrl":"","url":null,"abstract":"Trypanosoma musculi-macrophage co-cultures were studied to investigate the biological role of lipopolysaccharide (LPS) induced cytokines in controlling the proliferation of parasites in vitro. Macrophages, isolated by peritoneal lavage, sustained the growth and proliferation of the parasites. Macrophages activated with LPS were characterized by up-regulation of nitric oxide synthase (iNOS) and phagocytosis of fluorescent latex spheres. Activated macrophages showed marked inhibition of the association and proliferation of the parasites. The LPS treated macrophages produced cytokines, especially interferon gamma (INF-gamma), which was detected by Western blot. Trypanosomes, inhibited from association with macrophages, did not proliferate and instead formed clusters held together by their flagella. Cells in these clusters were apoptotic, as demonstrated by the Apoptag reaction and gel fragmentation assay. In addition, high levels of caspase 8 and caspase 3 were shown in floating trypanosome clusters. The results would suggest that INF-gamma and other cytokines released by activated macrophages, possibly functioning through the INF-gammaR1, Fas ligand, CD95 or other death ligands in the trypanosome plasma membrane initiates the apoptosis cascade in trypanosomes.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25730599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The myofibroblast: a study of normal, reactive and neoplastic tissues, with an emphasis on ultrastructure. Part 1--normal and reactive cells. 肌成纤维细胞:对正常、反应性和肿瘤组织的研究，重点是超微结构。第一部分，正常细胞和反应细胞。

Journal of submicroscopic cytology and pathology Pub Date : 2005-08-01

B Eyden

{"title":"The myofibroblast: a study of normal, reactive and neoplastic tissues, with an emphasis on ultrastructure. Part 1--normal and reactive cells.","authors":"B Eyden","doi":"","DOIUrl":"","url":null,"abstract":"The myofibroblast is essential for the integrity of the mammalian body by virtue of its role in wound-healing, but it can also threaten it by its ability to promote tumour progression. It is an almost universal cellular component in mammalian lesions, but not a typical component of normal untraumatised tissues. Partly because of its absence from normal tissue, it has not been part of conventional histology teaching. This has contributed to difficulties in appreciating the nature of the myofibroblast and defining it by scientists interested in the mechanism of disease and pathologists wanting to diagnose myofibroblastic rumours. This paper documents the features of the myofibroblast with an emphasis on ultrastructure. A base-line of understanding is first provided by a description of normal cells found in untraumatised tissues, from which the myofibroblast has on occasion been postulated as arising, or with which, to varying degrees, the myofibroblast has been confused--fibroblasts, smooth-muscle cells, endothelium, pericytes, myoepithelium and lymphoid reticulum cells. The biology, light microscopy features and ultrastructure of the myofibroblast are then documented for comparison. Features emphasised for defining the myofibroblast include: a spindled cell morphology, an abundant matrix, immunostaining for alpha-smooth-muscle actin (in the absence of desmin and h-caldesmon) and the ED-A splice variant of cellular fibronectin, rough endoplasmic reticulum, peripherally located smooth-muscle type myofilaments, a Golgi apparatus producing collagen-secretion granules, gap junctions and fibronexus junctions. The fibronexus is emphasised as a distinctive organelle for identifying the myofibroblast and lamina is emphasised as absent. The mechanism by which myofibroblasts arise in granulation tissue and promote tumour progression is discussed briefly, and an appendix provides summaries of the involvement of myofibroblasts in non-neoplastic diseases.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25742008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and ultrastructure of the spermatozoa of Halictidae (Hymenoptera, Apoidea). 膜翅目蜂科昆虫精子的结构和超微结构。

Journal of submicroscopic cytology and pathology Pub Date : 2005-04-01

B S Fiorillo, A A M Coelho, J Lino-Neto, S N Báo

{"title":"Structure and ultrastructure of the spermatozoa of Halictidae (Hymenoptera, Apoidea).","authors":"B S Fiorillo, A A M Coelho, J Lino-Neto, S N Báo","doi":"","DOIUrl":"","url":null,"abstract":"The spermatozoa in Halictidae are sometimes observed in spermatodesmata in the seminal vesicle. They are linear, long, slender and their lengths vary from 213 microm to about 330 pm. The head region consists in the anterior acrosomal complex, formed by a conical acrosomal vesicle that shows an inner paracrystalline perforatorium extending into the nucleus. The nucleus, measuring about 16 microm to 46 microm, is linear and strongly electron-dense, however some electron-lucent lacunae with electron-dense granules homogeneously organized were observed. The nucleus is attached to the flagellum by the centriolar adjunct, which is compact and electron-dense. It begins at the nuclear base and finishes just above the smaller mitochondrial derivative. The flagellum consists of two mitochondrial derivatives, an axoneme and two accessory bodies. Halictidae have an axoneme with 9+9+2 microtubule pattern which gradually disorganizes towards the final region. The mitochondrial derivatives are asymmetric in both length and diameter and only the larger presents the paracrystalline region. The typical pattern for Halictidae spermatozoa here described may provide useful additional information for future phylogenetic analysis of the superfamily Apoidea.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25278937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The evolution of the atheromatous plaque: ultrastructural evidence. 动脉粥样斑块的演变:超微结构证据。

Journal of submicroscopic cytology and pathology Pub Date : 2005-04-01

A Aleotti, P Boldrini, M R Bovolenta, F Cervellati, G Martines

引用次数: 0

Necrosis in human spermatozoa. II. Ultrastructural features and FISH study in semen from patients with recovered uro-genital infections. 人精子坏死。2泌尿生殖道感染恢复患者精液的超微结构特征和FISH研究。

Journal of submicroscopic cytology and pathology Pub Date : 2005-04-01

E Moretti, B Baccetti, S Capitani, G Collodel

{"title":"Necrosis in human spermatozoa. II. Ultrastructural features and FISH study in semen from patients with recovered uro-genital infections.","authors":"E Moretti, B Baccetti, S Capitani, G Collodel","doi":"","DOIUrl":"","url":null,"abstract":"Inflammation of the male genital tract is a potential cause of male sterility. The quality of spermatozoa from ten patients with recovered uro-genital infections was examined by transmission electron microscopy (TEM); fluorescence in situ hybridization (FISH) was performed on sperm nuclei in six our of ten patients to investigate the frequency of aneuploidies. TEM analysis demonstrated the presence of a high percentage of necrosis in all patients, whereas apoptosis was present in only five of them. Meiotic segregation was altered in all analysed semen samples. Recovery from infections does not seem to coincide with improved sperm quality, probably because a persistent inflammatory state demonstrated by a high percentage of sperm necrosis sometimes associated with the presence of white blood cells (WBC) in the seminal plasma, is present. The effects of infections of the male genital tract could proceed in the absence of microbial agents due to immunological mechanisms involving the pattern of chemical products typical of inflammation. Our results suggest that the presence of necrosis, sometimes associated with apoptosis, could be considered to be an indicator of male genital tract inflammation. However, further studies are necessary to test the correlation between biochemical parameters and ultrastructural and molecular markers of inflammation.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25278939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foci of amorphous/granulofilamentous matrix in the extracellular domain of tumours. 2. Immunohistochemical and immunogold characterization of a fibronectin-rich matrix component. 肿瘤细胞外区域的无定形/颗粒丝状基质灶。2. 富纤维连接蛋白基质组分的免疫组化和免疫金表征。

Journal of submicroscopic cytology and pathology Pub Date : 2005-04-01

B Eyden, I Shore, J Moss, K Yamazaki, Y Ru, J H Shanks, S S Banerjee

{"title":"Foci of amorphous/granulofilamentous matrix in the extracellular domain of tumours. 2. Immunohistochemical and immunogold characterization of a fibronectin-rich matrix component.","authors":"B Eyden, I Shore, J Moss, K Yamazaki, Y Ru, J H Shanks, S S Banerjee","doi":"","DOIUrl":"","url":null,"abstract":"The term FEAM (foci of extracellular amorphous matrix) has been used for discretely outlined areas of moderately dense material having a filamentous/granular substructure located in the extracellular matrix of tumours. In spite of being widespread in mesenchymal tumours especially, and often abundant, they have received little attention in terms of structure, composition and origin. Mostly, they have been regarded as a variant or a product of lamina ('basement membrane material'). However, they also appear in tumours whose cells should and do lack a lamina, such as giant-cell fibroblastoma and solitary fibrous tumour. This paper describes their fine structure in a variety of predominantly mesenchymal tumours, and documents their composition using light microscope immunostaining and immunogold labelling. Small amounts of type IV collagen and laminin were found focally and inconsistently among the five tumours by light microscope immunostaining, but fibronectin was strongly and consistently identified. Strong fibronectin staining was also identified by immuno-electronmicroscopy. These data suggest that FEAM represent a fibronectin-rich matrix constituent, which might be a common final product of either lamina or the external component of the subplasmalemmal linear density (focal adhesion). There is little support light microscopically for a relationship to immune-complexes or cryoglobulins.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25280302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural study of the blood cells of the coelacanth Latimeria chalumnae (Rhipidistia: Coelacanthini). 腔棘鱼血细胞的超微结构研究。

Journal of submicroscopic cytology and pathology Pub Date : 2005-04-01

M S Jarial

引用次数: 0