Journal of Spectroscopy最新文献_第5页

Feasibility Study on the Use of a Portable NIR Spectrometer and Multivariate Data Analysis to Discriminate and Quantify Adulteration in Fertilizer 便携式近红外光谱仪与多变量数据分析鉴别定量肥料掺假的可行性研究

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-11-29 DOI: 10.1155/2022/1412526

E. Teye, C. Amuah, K. Atiah, R. Darko, K. Amoah, E. Afutu, Rebecca Owusu

{"title":"Feasibility Study on the Use of a Portable NIR Spectrometer and Multivariate Data Analysis to Discriminate and Quantify Adulteration in Fertilizer","authors":"E. Teye, C. Amuah, K. Atiah, R. Darko, K. Amoah, E. Afutu, Rebecca Owusu","doi":"10.1155/2022/1412526","DOIUrl":"https://doi.org/10.1155/2022/1412526","url":null,"abstract":"The rise in population growth worldwide requires efficient management of agricultural lands through the correct determination of authentic fertilizers. In this current study, a rapid on-site detection technique was developed by using portable NIR spectroscopy in the wavelength range of 740–1070 nm together with optimum multivariate algorithms to identify fertilizer integrity (unexpired, expired, and adulterated) as well as quantify the levels (10–50%) of adulteration. NIR models were built based on support vector machine (SVM) and random forest (RF) for identification, while different types of partial least square regression (PLS, iPLS, Si-PLS, and GaPLS) were used for quantification purposes. The models were evaluated according to identification rate (Rt), coefficient of correlation in prediction (Rpre2), and root mean square error of prediction (RMSEP). For the identification of the integrity of the fertilizer, among the mathematical pretreatments used, the first derivative (FD) together with SVM gave above 99.20% identification rate in both calibration and prediction sets. For the quantification of the adulterants, Si-PLS was found to be superior and showed an excellent predictive potential of Rpre2 = 0.95–0.98 and RMSEP = 0.069–0.11 for the two fertilizer types used. The overall results indicated that a handheld NIR spectrometer together with appropriate algorithms could be employed for fast and on-site determination of fertilizer integrity.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"2 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80472150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reduction of Background Fluorescence from Impurities in Protein Samples for Raman Spectroscopy 拉曼光谱中蛋白质样品中杂质背景荧光的还原

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-11-24 DOI: 10.1155/2022/1928091

Marco Pinto Corujo, Pavel Michal, Rod Wesson, D. P. Amarasinghe, A. Rodger, N. Chmel

{"title":"Reduction of Background Fluorescence from Impurities in Protein Samples for Raman Spectroscopy","authors":"Marco Pinto Corujo, Pavel Michal, Rod Wesson, D. P. Amarasinghe, A. Rodger, N. Chmel","doi":"10.1155/2022/1928091","DOIUrl":"https://doi.org/10.1155/2022/1928091","url":null,"abstract":"Background fluorescence remains the biggest challenge in Raman spectroscopy because of the consequent curvature of the baseline and the degradation of the signal-to-noise ratio of the Raman signal. While the concentrations of the fluorophore impurities are usually too low to be detected by other analytical methods, they are often sufficient to prevent Raman data collection. Among the different existing methods to remove the fluorescence signal, photobleaching remains the most popular due to its simplicity. However, using the spectrometer laser to photobleach is far from optimal. Most commercially available instruments have little or no choice of wavelength, and their output powers are in many cases not suitable for highly fluorescent samples such as those from biological systems (e.g., proteins). In this article, we assess practical aspects of photobleaching such as the apparent reversibility of the process and the effect of convection currents due to what we speculate to be temperature gradients across the bulk of the solution. We also introduce an affordable custom made external photobleaching unit with a choice of excitation wavelength and demonstrate its viability with a highly fluorescent bovine serum albumin protein solution, which had proved most challenging for Raman spectroscopy as it contained ∼10% w/w impurities.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"133 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89067716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A New Method for Spectral Wavelength Selection Based on Multiple Linear Regression Combined with Ant Colony Optimization and Genetic Algorithm 一种结合蚁群优化和遗传算法的多元线性回归光谱波长选择新方法

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-11-22 DOI: 10.1155/2022/2440518

Qing Huang, Heru Xue, Jiangping Liu, Xinhua Jiang

{"title":"A New Method for Spectral Wavelength Selection Based on Multiple Linear Regression Combined with Ant Colony Optimization and Genetic Algorithm","authors":"Qing Huang, Heru Xue, Jiangping Liu, Xinhua Jiang","doi":"10.1155/2022/2440518","DOIUrl":"https://doi.org/10.1155/2022/2440518","url":null,"abstract":"Wavelength selection is one of the key steps in quantitative spectral analysis, which reduces the computation time while also improving the prediction accuracy of the model. In this paper, we propose a wavelength selection algorithm based on the ant colony optimization (ACO), in which the absolute value of the regression coefficient of the multiple linear regression (MLR) model is used as the basis for evaluating the importance of wavelengths, and the absolute value of the regression coefficient after full wavelength MLR modeling is used as the initial pheromone value of the ant colony optimization (MLR-ACO). In each iteration, the absolute value of the regression coefficient corresponding to each wavelength of the individual with the highest fitness value is used as the basis for a pheromone update. The crossover operator is introduced in MLR-ACO (MLR-ACO-GA), and the individuals with the top 100 fitness values in MLR-ACO are used as the initial population of the genetic algorithm (GA). A selected frequency of wavelengths greater than the threshold among MLR-ACO individuals is calculated. A number of coarse interval points are generated according to the selected frequency, and a coarse crossover operation is performed at the coarse interval points. Fine crossover points are randomly generated within the coarse interval, and fine crossover operations are performed within the coarse interval to exploit the potential of combining excellent individuals in MLR-ACO with each other as much as possible. MLR-ACO can well solve the problem of traditional ACO initial pheromone scarcity, and MLR-ACO-GA can avoid MLR-ACO falling into a local optimum to a certain extent and be more flexible in the selection of the number of wavelengths, which can give full play to the advantages of MLR-ACO.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"72 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79954278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Simple Determination of Gemifloxacin Levels in Human Plasma using High-Performance Liquid Chromatography-Tandem Mass Spectrometry 高效液相色谱-串联质谱法测定人血浆中吉氟沙星的含量

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-11-10 DOI: 10.1155/2022/8935929

Gaeun Kang, Sang Heon Suh, S. Ma, Jong-Keun Kim

{"title":"Simple Determination of Gemifloxacin Levels in Human Plasma using High-Performance Liquid Chromatography-Tandem Mass Spectrometry","authors":"Gaeun Kang, Sang Heon Suh, S. Ma, Jong-Keun Kim","doi":"10.1155/2022/8935929","DOIUrl":"https://doi.org/10.1155/2022/8935929","url":null,"abstract":"Gemifloxacin, a broad-spectrum antibacterial agent of the fluoroquinolone class, is used to treat bacterial infections, including acute bacterial exacerbation of chronic bronchitis and community-acquired pneumonia. This study aimed to develop a simple and robust analysis of gemifloxacin in human plasma by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The sample was prepared using simple protein precipitation procedures with acetonitrile and separated using the Gemini C18 column with a mobile phase (0.1% formic acid: 0.1% formic acid in acetonitrile = 78: 22 (V/V)). Moxifloxacin was used as an internal standard. The mass spectrometer was operated in the positive ion mode using multiple reaction monitoring. Each precursor ion of gemifloxacin and moxifloxacin was monitored at m/z 390.1/402.1, and their product ions were monitored at m/z 372.3/384.4. The calibration curve showed linearity in 0.005–5 μg/mL with an appropriate correlation coefficient (≥0.99). The variation coefficient of intra- and interprecision values of gemifloxacin was <15%. The intra- and interaccuracy values ranged from 85% to 115%, except for the lower limit of quantification (accuracy range: 80%–120%). The proposed method was performed with a simple preparation step, and moxifloxacin, which is easily accessible, was used as the internal standard. These results suggest that the present assay is a practical analytical method and, therefore, can be readily applied for analysis, including in pharmacokinetic studies and therapeutic drug monitoring of gemifloxacin.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"72 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76545477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of a Peak-Free Chemometric Laser-Induced Breakdown Spectroscopy Method for Direct Rapid Cancer Detection via Trace Metal Biomarkers in Tissue 利用组织中痕量金属生物标志物直接快速检测癌症的无峰化学测量激光诱导击穿光谱方法的评价

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-10-26 DOI: 10.1155/2022/1874173

Otieno Emily Akinyi, A. Kalambuka, A. Dehayem-kamadjeu

{"title":"Evaluation of a Peak-Free Chemometric Laser-Induced Breakdown Spectroscopy Method for Direct Rapid Cancer Detection via Trace Metal Biomarkers in Tissue","authors":"Otieno Emily Akinyi, A. Kalambuka, A. Dehayem-kamadjeu","doi":"10.1155/2022/1874173","DOIUrl":"https://doi.org/10.1155/2022/1874173","url":null,"abstract":"The ability to perform direct rapid analysis in air and at atmospheric pressure is a remarkable attraction of laser-induced breakdown spectroscopy (LIBS) for the diagnostic quantification of disease biomarker metals in body tissue. However, accurate trace analysis is limited by matrix effects and a pronounced background that masks the subtle (peak-free) analyte signals because tissue plasma is dense and most lines are optically thick. In this work, a peak-free chemometric LIBS method based on a single-shot (for rapidity and nondestructiveness) and an artificial neural network multivariate calibration strategy with spectral feature selection was evaluated for its utility for direct trace quantitative analysis of copper (Cu), iron (Fe), manganese (Mg), magnesium (Mg), and zinc (Zn) in model soft body tissue. The spectral signatures corresponding to the biometals (so-called because the metals are intrinsic to tissue biochemistry) were generated by spiking their known human-body-representative concentrations in molten paraffin wax. The developed multivariate analytical model achieved ≥95% accuracy as determined from the analysis of oyster tissue-certified reference material. The analytical models were tested on the liver, breast, and abdominal tissue biopsies. The results of applying the model to the clinical tissues indicated the absence or presence (including severity) of cancer as either malignant or benign, in agreement with the pathological examination report.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"88 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90357963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices 酸橙汁中酚类化合物的光谱色谱测定

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-10-22 DOI: 10.1155/2022/9228394

F. Shakeri, M. Shakeri, Fatemeh Pour Ramezani, Mohadeseh Pirhadi, Masoud Aman Mohammadi, S. Shokri, T. Zeinali

{"title":"Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices","authors":"F. Shakeri, M. Shakeri, Fatemeh Pour Ramezani, Mohadeseh Pirhadi, Masoud Aman Mohammadi, S. Shokri, T. Zeinali","doi":"10.1155/2022/9228394","DOIUrl":"https://doi.org/10.1155/2022/9228394","url":null,"abstract":"Juice demand has been increasing at a rapid rate in recent years, and one of the major efforts underway to meet this demand is to minimize changes during the juice process. Due to the high consumption of juice and the carcinogenicity of synthetic and chemical substances, this research uses high performance liquid chromatography (HPLC) and spectrophotometry to detect fake juices. For the detection of fraud, the tests of sodium and potassium content along with determining the amount of flavonoids (hesperidin and eriocitrin) were carried out using spectrophotometry and HPLC. The results showed the average amount of total polyphenol was from 32.4 to 42.6 mg L-1. The total polyphenol content in all samples conformed to the standard, and there was no significant difference between the samples and the standard. The amounts of flavonoids (hesperidin and eriocitrin) in the juice samples were below the standard level (a minimum of 90 and 20 g/mL, respectively). Also, there was a significant difference between the mean sodium and potassium content of standard versus feigned juices. Generally, the amount of hesperidin, eriocitrin, Na+, and K+ as diagnostic biomarkers of natural juice in all samples was below the standard level. All the analysed samples in the experiment were nonstandard. There is a lot of fraud in the juice business, so it has been suggested that the government should have more control over how manufacturing companies make juice.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"5 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73591186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Study on the Quantitative Assessment of Impact Damage of Yellow Peaches Using the Combined Hyperspectral Technology and Mechanical Parameters 利用高光谱技术和力学参数联合定量评价黄桃冲击损伤的研究

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-10-14 DOI: 10.1155/2022/7526826

Feng Zhang, Bin Li, Hai-Long Yin, Jiping Zou, Aiguo Ouyang

{"title":"Study on the Quantitative Assessment of Impact Damage of Yellow Peaches Using the Combined Hyperspectral Technology and Mechanical Parameters","authors":"Feng Zhang, Bin Li, Hai-Long Yin, Jiping Zou, Aiguo Ouyang","doi":"10.1155/2022/7526826","DOIUrl":"https://doi.org/10.1155/2022/7526826","url":null,"abstract":"The quantitative description of the impact damage of yellow peaches is an essential basis for evaluating their quality and guiding their postharvest handling. In this study, the combined hyperspectral technology and mechanical parameters method were used to quantitatively investigate the impact damage of yellow peaches. Firstly, the mechanical parameters, which are damaged area, absorbed energy, maximum contact force, and maximum stress of yellow peaches, were obtained by the impact device. The statistical regression models between mechanical parameters and damage area were established, and the results showed that the absorbed energy and maximum contact force are the optimal parameters to characterize the impact damage of yellow peaches. Then, the raw spectra were preprocessed by three spectral pretreatment methods, which are standard normal variate (SNV), multiplicative scatter correction (MSC), and SG smoothing, respectively, and the feature wavelengths were selected by the competitive adaptive reweighted sampling (CARS), and the quantitative relationships between spectra and mechanical parameters were successfully modeled based on partial least squares regression (PLSR). The results showed that there is a strong linear correlation between the spectral data and the mechanical parameters, and the prediction performance of the SNV-CARS-PLSR model is best, and the RP and RMSEP of the damaged area, absorbed energy, maximum contact force, and maximum stress of it were 0.920 and 86.452 mm2, 0.845 and 1.303 J, 0.943 and 49.666 N, 0.660 and 0.146 MPa, respectively. In a word, this study shows that the combined hyperspectral technology and mechanical parameters method can be used to quantitatively assess the impact damage of yellow peaches, and guide the postharvest handling of fruits.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"8 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78484675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Quantitative Raman Spectroscopic Determination of the Composition, Pressure, and Density of CO2-CH4 Gas Mixtures 定量拉曼光谱测定CO2-CH4气体混合物的组成、压力和密度

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-09-30 DOI: 10.1155/2022/7238044

Ying Chen, I. Chou

{"title":"Quantitative Raman Spectroscopic Determination of the Composition, Pressure, and Density of CO2-CH4 Gas Mixtures","authors":"Ying Chen, I. Chou","doi":"10.1155/2022/7238044","DOIUrl":"https://doi.org/10.1155/2022/7238044","url":null,"abstract":"The Raman spectra for pure CO2 and CH4 gases and their ten gas mixtures were collected at pressures and temperatures ranging from 2 MPa to 40 MPa and room temperature (∼24°C) to 300°C, respectively. A systematic analysis was carried out to establish a methodology for the quantitative determination of the composition, pressure, and density of CO2-CH4 mixtures. The shift in the peak position of the υ1 band for CH4 was sufficiently large to enable the accurate determination of the pressure of pure CH4 and CH4-dominated fluids (>50 mol% CH4). An equation representing the observed relationship of the peak position of the υ1 band of CH4, density, and composition was developed to calculate the density of CO2-CH4 mixtures. The Raman quantification factor F (CH4)/F (CO2) was demonstrated to be near a constant value of 5.048 ± 0.4 and was used to determine the CH4 to CO2 molar ratio in an unknown CO2-CH4‐bearing fluid with high internal pressure (>10 MPa) based on the Raman peak area ratio. The effect of temperature on the variation in Raman spectral parameters was also investigated at temperatures up to 300°C. The results showed that the effect of temperature must be considered when Raman spectral parameters are used to calculate the pressure, density, and composition of CO2-CH4 gas mixtures. Raman spectroscopic analysis results obtained for six samples prepared in fused silica capillary capsules were validated by comparison with the results obtained from microthermometry measurements.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"51 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79133427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Spectroscopic Study on the Species and Color Differences of Gem-Quality Red Garnets from Malawi 马拉维宝石级红石榴石的种类和颜色差异的光谱研究

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-09-27 DOI: 10.1155/2022/1638042

Ming Li

{"title":"Spectroscopic Study on the Species and Color Differences of Gem-Quality Red Garnets from Malawi","authors":"Ming Li","doi":"10.1155/2022/1638042","DOIUrl":"https://doi.org/10.1155/2022/1638042","url":null,"abstract":"To reveal the species of gem-grade red garnets with similar colors, especially the mechanisms underlying their subtle color differences, a series of tests, including conventional gemological tests, X-ray diffraction, Fourier-transform infrared spectroscopy, ultraviolet-visible spectroscopy, and X-ray photoelectron spectroscopy, were performed on gem-grade red garnets mined from Malawi. The results demonstrated that the color difference is not caused by the difference in species, and both the purplish-red and maroon-red garnets were magnesium-aluminum garnets (pyrope). They both contained the transition metal ions Fe2+, Mn2+, Fe3+, and Cr3+, with Fe2+ and Mn2+ occupying crystal site A and Fe3+ and Cr3+ occupying crystal site B. Cr3+ absorption peaks were observed at 367 and 690 nm; Fe3+ absorption peaks were observed at 502, 528, and 570 nm; and Mn2+ absorption peaks were observed at 400, 423, and 460 nm, which contributed to their respective colors. However, while the maroon-red pyrope had a larger Fe2+/Fe3+ ratio than the purplish-red pyrope, it lacked Mn2+ ions, which is the cause of the color difference between the two pyrope garnets. To date, the study of color differences in red garnets remains a major controversial topic. This study proposed an innovative spectroscopic approach, particularly the combination of ultraviolet-visible spectroscopy and X-ray photoelectron spectroscopy, thus providing a novel methodology for investigating color differences in red garnets.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"37 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86779736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine 中草药中地塞米松含量测定的简易分析方法

IF 2 4区化学

Journal of Spectroscopy Pub Date : 2022-09-27 DOI: 10.1155/2022/5141647

Rimadani Pratiwi, Adira Rahmawaty, A. N. Hasanah

{"title":"Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine","authors":"Rimadani Pratiwi, Adira Rahmawaty, A. N. Hasanah","doi":"10.1155/2022/5141647","DOIUrl":"https://doi.org/10.1155/2022/5141647","url":null,"abstract":"Chemical drugs have been added to some of the traditional herbal medicine to enhance the therapeutic effect. One of them is dexamethasone which is added in herbal pain relief. To determine the content of dexamethasone in herbal medicine, a quantitative analytical method was developed with the reaction of the formation of a dexamethasone-hydroxylamine complex with fluoranil which was measured by UV-Vis spectrophotometry. Under optimized conditions, the performance of this system to analyze dexamethasone exhibited a good analytical response. The correlation coefficient was (R2) of 0.9965 from a concentration of 1–40 g/mL, LOD, and LOQ of 0.21 μg/mL and 0.64 μg/mL, % recovery for concentrations of 80%,100%, and 120%, are 100.14% ± 0.58; 98.35% ± 1.19; and 99.00% ± 1.18, respectively; and %RSD is 0.75%. The sixteen herbal medicine samples were analyzed to confirm the application of this method. The result showed six samples confirmed containing dexamethasone with levels ranging from 7.44 to 21.61 μg/mL and confirmed with HPLC data. The UV-Vis spectrophotometry method is simple and effective for dexamethasone analysis in herbal medicine.","PeriodicalId":17079,"journal":{"name":"Journal of Spectroscopy","volume":"53 74 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83061478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1