中草药中地塞米松含量测定的简易分析方法

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-09-27 DOI:10.1155/2022/5141647

Rimadani Pratiwi, Adira Rahmawaty, A. N. Hasanah

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引用次数: 1

摘要

一些传统的草药中加入了化学药物以增强治疗效果。其中一种是地塞米松，它被添加到草药止痛剂中。为测定中草药中地塞米松的含量，建立了用紫外-可见分光光度法测定地塞米松-羟胺配合物与氟腈反应的定量分析方法。在优化条件下，该系统对地塞米松的分析效果良好。在浓度为1 ~ 40 g/mL时，LOD和LOQ分别为0.21和0.64 μg/mL，相关系数为(R2) 0.9965;在浓度为80%、100%和120%时，回收率分别为100.14%±0.58;98.35%±1.19;分别为99.00%±1.18;%RSD为0.75%。对16份药材样品进行分析，验证该方法的适用性。结果证实6份样品含有地塞米松，含量范围为7.44 ~ 21.61 μg/mL。紫外可见分光光度法简便、有效地测定中草药中地塞米松的含量。

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Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine

Chemical drugs have been added to some of the traditional herbal medicine to enhance the therapeutic effect. One of them is dexamethasone which is added in herbal pain relief. To determine the content of dexamethasone in herbal medicine, a quantitative analytical method was developed with the reaction of the formation of a dexamethasone-hydroxylamine complex with fluoranil which was measured by UV-Vis spectrophotometry. Under optimized conditions, the performance of this system to analyze dexamethasone exhibited a good analytical response. The correlation coefficient was (R2) of 0.9965 from a concentration of 1–40 g/mL, LOD, and LOQ of 0.21 μg/mL and 0.64 μg/mL, % recovery for concentrations of 80%,100%, and 120%, are 100.14% ± 0.58; 98.35% ± 1.19; and 99.00% ± 1.18, respectively; and %RSD is 0.75%. The sixteen herbal medicine samples were analyzed to confirm the application of this method. The result showed six samples confirmed containing dexamethasone with levels ranging from 7.44 to 21.61 μg/mL and confirmed with HPLC data. The UV-Vis spectrophotometry method is simple and effective for dexamethasone analysis in herbal medicine.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.