Journal of Reports in Pharmaceutical Sciences最新文献_第6页

Evaluation of total phenolics, flavonoids, and antioxidant and cytotoxic potential of Ailanthus altissima (Mill.) swingle leaves 臭椿单叶总酚、总黄酮、抗氧化和细胞毒潜能的评价

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.JRPTPS_7_21

H. Mohamed, E. El-wakil, E. Abdel-hameed, M. El-hashash, Mohamed A Shemis

{"title":"Evaluation of total phenolics, flavonoids, and antioxidant and cytotoxic potential of Ailanthus altissima (Mill.) swingle leaves","authors":"H. Mohamed, E. El-wakil, E. Abdel-hameed, M. El-hashash, Mohamed A Shemis","doi":"10.4103/jrptps.JRPTPS_7_21","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_7_21","url":null,"abstract":"Context: People all over the world are suffering from cancer. Liver cancer is considered the second most common malignancy among Egyptian men and the sixth most common malignancy among Egyptian women. Plant-derived antioxidants are believed to prevent or delay the occurrence of many chronic diseases such as cancer. Ailanthus altissima has been used in many traditional prescriptions. Aims: The current study aimed at investigating the phytochemical profile of A. altissima leaves’ extract and its derived fractions, determining their content of phenolics and flavonoids as well as assessing their antioxidant and cytotoxic potential. Materials and Methods: The phytochemical screening was carried out using standard methods. The total phenolic, flavonoid, and flavonol contents were determined using Folin-Ciocalteu, aluminum chloride, and aluminum chloride/ sodium acetate assays, respectively. The antioxidant activity was evaluated using different in vitro methods: DPPH•, total antioxidant capacity, hydroxyl (•OH), nitric oxide (NO•) radical scavenging activities, and permanganate-reducing antioxidant capacity (PRAC). The antiproliferative potential against HepG2 cells was evaluated using sulforhodamine-B assay (SRB). Results: The results showed that the ethyl acetate fraction had the highest content of phenolics, flavonoids, and flavonols (551.72 ± 1.81 mg GAE/g ext., 371.24 ± 4.36 mg RE/g ext., and 100.47 ± 1.30 mg QE/g ext., respectively). It also had the most potent reducing power (DPPH• SC50 = 7.19 ± 0.05 µg/mL, TAC= 369.88 ± 1.51 mg AAE/g ext., •OH SA = 95.46 ± 0.14%, NO• SA = 40.65 ± 0.91%, and PRAC = 77.19 ± 0.27%). The n-butanol fraction exhibited the most potent cytotoxic potential against HepG2 cells (IC50 = 16.70 µg/mL). Conclusion: A. altissima leaves could be considered potent antioxidant and cytotoxic alternatives.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45550717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Simultaneous RP-HPLC estimation and validation of metronidazole, furazolidone, and dicyclomine in capsule RP-HPLC同时测定胶囊中甲硝唑、呋喃唑酮和二环胺的含量

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.JRPTPS_104_19

Amuthalakshmi Sivaperuman, N. Nagarajan, Uma Jayapaul

{"title":"Simultaneous RP-HPLC estimation and validation of metronidazole, furazolidone, and dicyclomine in capsule","authors":"Amuthalakshmi Sivaperuman, N. Nagarajan, Uma Jayapaul","doi":"10.4103/jrptps.JRPTPS_104_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_104_19","url":null,"abstract":"For the first time, a new, simple, precise reversed-phase high-performance liquid chromatography method was developed for the simultaneous estimation of metronidazole, furazolidone, and dicyclomine hydrochloride in capsule dosage form. The method was performed with Thermo, C8 (150 mm×4.6) column. The best separation was achieved by gradient elution with mobile phase of acetonitrile, water (40:60), and 20 mm phosphate buffer with 10% w/v sodium hydroxide (pH 7.5) with a detection wavelength of 215 nm. The separation was completed within 15 min of runtime. The retention time of metronidazole, furazolidone, and dicyclomine hydrochloride was found to be 1.79, 2.45, and 11.50 min, respectively. The proposed method was found to be linear. The method was statistically validated as per the ICH guidelines and shown to be simple, accurate, precise, linear, and reproducible in the range of 40.2–60, 40.2–60.4, and 3–5 µg/mL for metronidazole, furazolidone, and dicyclomine, respectively. For the first time, the developed method foretells the suitability of the method for the simultaneous estimation of three drugs in the commercially available dosage forms.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42252231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Attenuation of hyperinsulinemia-induced DNA damage of peripheral lymphocytes by carvedilol 卡维地洛对高胰岛素诱导的外周血淋巴细胞DNA损伤的抑制作用

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.jrptps_1_21

M. Etebari, Farshad Naghsh-Nilchi

引用次数: 0

Study of monoamine oxidase inhibitory effects of seven Iranian medicinal plant extracts 7种伊朗药用植物提取物抑制单胺氧化酶作用的研究

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.jrptps_23_20

M. Soodi, Mohsen Shamsi, H. Hajimehdipoor, A. Ghazanfari

引用次数: 0

Immunoregulatory effects of paroxetine in healthy volunteers: An in vitro investigation 帕罗西汀对健康志愿者免疫调节作用的体外研究

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.JRPTPS_66_20

Alireza Karimollah, Anahid Hemmatpur, M. Asadi, Mohammad Hadinedoushan

{"title":"Immunoregulatory effects of paroxetine in healthy volunteers: An in vitro investigation","authors":"Alireza Karimollah, Anahid Hemmatpur, M. Asadi, Mohammad Hadinedoushan","doi":"10.4103/jrptps.JRPTPS_66_20","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_66_20","url":null,"abstract":"Background: Paroxetine has been a commonly prescribed antidepressant for treatment of major depression and various anxiety disorders for almost 30 years due to its fewer side effects and toxicity compared with its counterparts. Despite several investigations performed, the paroxetine immunoregulatory effect in healthy subjects is still controversial. In this study, the paroxetine effect on the cell proliferation along with IL-4 and interferon-gamma (IFN-γ) secretion in peripheral blood mononuclear cells (PBMCs) of physically and mentally healthy subjects is investigated. Materials and Methods: Blood was drawn from 20 healthy subjects and PBMCs were isolated. Cells were treated with paroxetine and/or phytohemagglutinin (PHA) for 72 h. IL-4 and IFN-γ concentrations were assessed in the supernatant using an enzyme-linked immunosorbent assay. The BrdU cell proliferation assay was also performed to evaluate the paroxetine effect on PBMCs in the absence or presence of PHA. Results: Paroxetine (25 μM) significantly inhibited the production of IL-4 and IFN-γ in PHA-stimulated human PBMC cultures. Surprisingly, paroxetine suppressed cell proliferation in the unstimulated culture in a dose-independent manner. Paroxetine also attenuated cell proliferation in the PHA-stimulated culture, especially at 25 μM concentration. Conclusion: The obtained results suggest that paroxetine can be a potent therapeutic option in inflammatory diseases by balancing immune responses.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45642259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory effects of memantine in carrageenan-induced paw edema model in rats 美金刚对卡拉胶致大鼠足跖水肿模型的抗炎作用

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.JRPTPS_37_20

Mahtab Azarbaijani, Mehdi Kian, H. Soraya

{"title":"Anti-inflammatory effects of memantine in carrageenan-induced paw edema model in rats","authors":"Mahtab Azarbaijani, Mehdi Kian, H. Soraya","doi":"10.4103/jrptps.JRPTPS_37_20","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_37_20","url":null,"abstract":"Background: The anti-inflammatory effect of memantine (MEM) was investigated using carrageenan-induced hind paw edema model in rats. Materials and Methods: Thirty male Wistar rats were randomly assigned to five groups (n = 6). Group 1 received 0.1 mL of 1% carrageenan at the right hind paw. Group 2 received dexamethasone (10 mg/kg) and Groups 3, 4, and 5 received 5, 10, and 20 mg/kg MEM intraperitoneally (ip), 20 min after injection of carrageenan into the right hind paw, respectively. Animals’ paw thickness was measured at 0, 1, 2, 3, 4, and 5 h after carrageenan injection. Then, animals were euthanized and myeloperoxidase (MPO) and malondialdehyde (MDA) levels were measured in the paw tissues. The tissue samples were further examined histopathologically using light microscopy. One-way ANOVA and Tukey post hoc test was used to compare the mean values between the groups. Results: Treating with MEM at all doses significantly decreased hind paw thickness at 2 (P < 0.05 and P < 0.01 at MEM 10 mg/kg and MEM 5 and 20 mg/kg, respectively), 3 (P < 0.001), and 4 (P < 0.001 at 5 mg/kg and P < 0.01 at MEM 10 and 20 mg/kg) hours after carrageenan injection in comparison to the carrageenan group. There was a significant (P < 0.05 and P < 0.001, respectively) reduction in MPO activity and MDA levels in MEM-treated groups when compared with the carrageenan group. Conclusion: This study showed that MEM decreased paw edema, leukocyte infiltration, MPO activity, and MDA levels, and MEM can be considered as an effective anti-inflammatory agent.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44031736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hepatoprotective effects of Hertia cheirifolia butanolic extract and selenium against CCl4-induced toxicity in rats 何首乌丁醇提取物和硒对ccl4致大鼠肝保护作用的研究

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.jrptps_25_20

Mouna Menakh, Saber Boutellaa, D. Mahdi, A. Zellagui, M. Lahouel, M. Ozturk

引用次数: 0

Quantitative structure–activity relationship modeling of some naphthoquinone derivatives as inhibitors of pathogenic agent IDO1 一些萘醌衍生物作为致病菌IDO1抑制剂的定量构效关系模型

IF 0.6

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.jrptps_124_20

S. Asadpour, Sajjad Jazayeri Farsani, A. Semnani, Shima Ghanavati Nasab

引用次数: 0

A review on novel topical formulations of vitamins 新型维生素外用制剂的研究进展

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Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.jrptps_91_20

R. Mallya, Juhi Desai

引用次数: 1

A review on solubility enhancement methods for poorly water-soluble drugs 难溶性药物溶解度增强方法综述

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Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.4103/jrptps.JRPTPS_134_19

A. Ainurofiq, D. Putro, Dhea A. Ramadhani, Gemala Putra, Laura Do Espirito Santo

引用次数: 14