Evaluation of total phenolics, flavonoids, and antioxidant and cytotoxic potential of Ailanthus altissima (Mill.) swingle leaves

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
H. Mohamed, E. El-wakil, E. Abdel-hameed, M. El-hashash, Mohamed A Shemis
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引用次数: 4

Abstract

Context: People all over the world are suffering from cancer. Liver cancer is considered the second most common malignancy among Egyptian men and the sixth most common malignancy among Egyptian women. Plant-derived antioxidants are believed to prevent or delay the occurrence of many chronic diseases such as cancer. Ailanthus altissima has been used in many traditional prescriptions. Aims: The current study aimed at investigating the phytochemical profile of A. altissima leaves’ extract and its derived fractions, determining their content of phenolics and flavonoids as well as assessing their antioxidant and cytotoxic potential. Materials and Methods: The phytochemical screening was carried out using standard methods. The total phenolic, flavonoid, and flavonol contents were determined using Folin-Ciocalteu, aluminum chloride, and aluminum chloride/ sodium acetate assays, respectively. The antioxidant activity was evaluated using different in vitro methods: DPPH•, total antioxidant capacity, hydroxyl (•OH), nitric oxide (NO•) radical scavenging activities, and permanganate-reducing antioxidant capacity (PRAC). The antiproliferative potential against HepG2 cells was evaluated using sulforhodamine-B assay (SRB). Results: The results showed that the ethyl acetate fraction had the highest content of phenolics, flavonoids, and flavonols (551.72 ± 1.81 mg GAE/g ext., 371.24 ± 4.36 mg RE/g ext., and 100.47 ± 1.30 mg QE/g ext., respectively). It also had the most potent reducing power (DPPH• SC50 = 7.19 ± 0.05 µg/mL, TAC= 369.88 ± 1.51 mg AAE/g ext., •OH SA = 95.46 ± 0.14%, NO• SA = 40.65 ± 0.91%, and PRAC = 77.19 ± 0.27%). The n-butanol fraction exhibited the most potent cytotoxic potential against HepG2 cells (IC50 = 16.70 µg/mL). Conclusion: A. altissima leaves could be considered potent antioxidant and cytotoxic alternatives.
臭椿单叶总酚、总黄酮、抗氧化和细胞毒潜能的评价
背景:世界各地的人们都在遭受癌症的折磨。癌症被认为是埃及男性中第二常见的恶性肿瘤,埃及女性中第六常见的恶性疾病。植物来源的抗氧化剂被认为可以预防或延缓许多慢性疾病的发生,如癌症。臭椿被用于许多传统方剂中。目的:本研究旨在研究高铁叶提取物及其衍生部分的植物化学特征,测定其酚类和黄酮类化合物的含量,并评估其抗氧化和细胞毒性潜力。材料与方法:采用标准方法进行植物化学筛选。分别使用Folin-Ciocalteu、氯化铝和氯化铝/乙酸钠测定法测定总酚、类黄酮和黄酮醇含量。使用不同的体外方法评估抗氧化活性:DPPH•、总抗氧化能力、羟基(•OH)、一氧化氮(NO•)自由基清除活性和高锰酸盐还原抗氧化能力(PRAC)。使用磺基罗丹明-B测定法(SRB)评估对HepG2细胞的抗增殖潜力。结果:乙酸乙酯组分的酚类、黄酮类和黄酮醇含量最高(551.72±1.81) mg GAE/g ext.,371.24±4.36 mg RE/g ext.和100.47±1.30 mg QE/g ext.)。它也具有最强的还原力(DPPH•SC50=7.19±0.05µg/mL,TAC=369.88±1.51 mg AAE/g ext.,•OH SA=95.46±0.14%,NO•SA=40.65±0.91%,PRAC=77.19±0.27%)。正丁醇组分对HepG2细胞表现出最强的细胞毒性潜力(IC50=16.70µg/mL)。结论:高铁叶可作为有效的抗氧化剂和细胞毒性替代品。
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
0.00%
发文量
0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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