Journal of receptor research最新文献_第8页

Primary culture of canine tracheal smooth muscle cells in serum-free medium: effects of insulin-like growth factor I and insulin. 犬气管平滑肌细胞在无血清培养基中的原代培养:胰岛素样生长因子I和胰岛素的影响。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073702

C M Yang, S P Chou

{"title":"Primary culture of canine tracheal smooth muscle cells in serum-free medium: effects of insulin-like growth factor I and insulin.","authors":"C M Yang, S P Chou","doi":"10.3109/10799899309073702","DOIUrl":"https://doi.org/10.3109/10799899309073702","url":null,"abstract":"The effects of growth factors on cell growth and muscarinic receptor (mAChR) expression of canine tracheal smooth muscle cells (TSMCs) were observed under serum-free medium supplemented with 0.1% BSA. In the presence of 0.1% BSA, TSMCs withdraw from cell cycle as compared with 10% FBS and allow to determine the effects of growth factors on mAChR expression. The individual components of growth factors (IGF-I, insulin, and aFGF) at the concentration used are not sufficient to stimulate growth of TSMCs in the primary culture with 0.1% BSA. IGF-I (10 ng/ml) and insulin (1 microgram/ml), alone or in combination, could stimulate the expression of mAChRs of cultured TSMCs. Heparin could inhibit these stimulatory effects of mAChR expression. The stimulatory effects of IGF-I and insulin on mAChR expression were mediated through their own receptors since these effects were reversed by pretreatment of TSMCs with antibodies of the respective growth factor receptors. The pharmacological response of functional mAChRs, determined as accumulation of inositol phosphates induced by carbachol, is greater in the medium containing IGF-I and insulin than that cultured in 0.1% BSA. These results firmly establish that IGF-I and insulin could stimulate the expression of mAChRs in TSMCs under serum-free culture condition.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 6","pages":"943-60"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19492643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Modulation of calcitonin binding by calcium: differential effects of divalent cations. 钙对降钙素结合的调节:二价阳离子的差异效应。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309063271

S D Stroop, E E Moore, R E Kuestner, D L Thompson

{"title":"Modulation of calcitonin binding by calcium: differential effects of divalent cations.","authors":"S D Stroop, E E Moore, R E Kuestner, D L Thompson","doi":"10.3109/10799899309063271","DOIUrl":"https://doi.org/10.3109/10799899309063271","url":null,"abstract":"Binding of salmon calcitonin to bovine hypothalamic membranes is enhanced about 25% by calcium with a half-maximal effect at 15 mM calcium. In contrast, membranes prepared from a cell line expressing a recombinant human calcitonin receptor show no effect of calcium under similar conditions. The hypothalamic calcitonin receptor solubilized with CHAPS detergent retains an apparent Kd of 0.3 nM for salmon calcitonin; however, binding of calcitonin to the detergent-solubilized receptor complex can be inhibited by divalent cations in order of potency Mn > Ca approximately Sr approximately Mg >> NaCl with Mn and Ca having apparent Ki's of 5 mM and 20 mM respectively. Dixon and Scatchard plots of Mn and Ca inhibition of binding to the soluble receptor complex suggest a noncompetitive mechanism of inhibition. Calcium also inhibits calcitonin binding to a detergent-solubilized recombinant human calcitonin receptor. Inhibition of calcitonin binding is observed using two independent methods for determining soluble receptor-hormone complex and inhibition is reversed by EDTA.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 8","pages":"1173-97"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309063271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19242502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Rhodopsin phosphorylation by transiently expressed human beta ARK1: a new method for drug development. 瞬时表达人β - ARK1的视紫红质磷酸化:一种药物开发的新方法。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073648

G Parruti, M S Lombardi, T T Chuang, A De Blasi

引用次数: 7

Structure functional expression and spatial distribution of a cloned cDNA encoding a rat 5-HT1D-like receptor. 大鼠5- ht1d样受体cDNA克隆的结构、功能表达及空间分布。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073674

A W Bach, L Unger, R Sprengel, G Mengod, J Palacios, P H Seeburg, M M Voigt

{"title":"Structure functional expression and spatial distribution of a cloned cDNA encoding a rat 5-HT1D-like receptor.","authors":"A W Bach, L Unger, R Sprengel, G Mengod, J Palacios, P H Seeburg, M M Voigt","doi":"10.3109/10799899309073674","DOIUrl":"https://doi.org/10.3109/10799899309073674","url":null,"abstract":"Using polymerase chain reaction (PCR) a complementary DNA (cDNA) encoding a 5-hydroxytryptamine (5-HT) receptor was isolated from rat forebrain. The amplified cDNA specifies an open reading frame of 374 amino acids comprising seven putative transmembrane regions. Expression of the cloned cDNA in human embryonic kidney cells (HEK 293) was used to establish the pharmacological profile of the encoded receptor polypeptide. Membranes containing the cloned receptor showed high affinity binding of [3H]-5-HT. Competition binding experiments with a variety of serotonin receptor ligands displayed a rank order of affinities corresponding to a 5-HT1D subtype: 5-CT > 5-HT = metergoline > CGS 12066 > methysergide > sumatriptan > mianserin = (-)alpha-Me-5-HT = yohimbine > 8-OH-DPAT > or = rauwolscine > spiperone > DOI > propranolol > or = 2-Me-5-HT > or = ICS 205930. Ketanserin and ritanserin displaced [3H]-5-HT-binding in a biphasic manner. In situ hybridization revealed highest expression of the corresponding mRNA in the pyramidal layer of the olfactory tubercle and the nucleus caudatus and accumbens.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 1-4","pages":"479-502"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19434381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Insulin and IGE-1 receptors in a human intestinal adenocarcinoma cell line (CACO-2): regulation of Na+ glucose transport across the brush border. 人肠腺癌细胞系(CACO-2)中胰岛素和IGE-1受体:Na+葡萄糖在刷状边界运输的调节

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309063266

R S MacDonald, W H Thornton, T L Bean

{"title":"Insulin and IGE-1 receptors in a human intestinal adenocarcinoma cell line (CACO-2): regulation of Na+ glucose transport across the brush border.","authors":"R S MacDonald, W H Thornton, T L Bean","doi":"10.3109/10799899309063266","DOIUrl":"https://doi.org/10.3109/10799899309063266","url":null,"abstract":"Both insulin and IGF-1 receptors are present in intestinal mucosal cells, although their role in this tissue is unclear. We have characterized these receptors in a human adenocarcinoma cell line, Caco-2, and examined their role in the regulation of glucose transport and absorption in these cells. The Caco-2 cells demonstrated specific insulin and IGF-1 receptors. They also bound cytochalasin B, suggesting the presence of a glucose transporter-like protein. When grown on membranes, the Caco-2 cells formed columnar, bipolar cells with tight junctions. The monolayer selectively transported D-glucose and methyl-D-glucose, with complete exclusion of L-glucose, D-mannitol and inulin. The absorption of glucose across the monolayer occurred via a Na+/glucose cotransporter, as indicated by a change in short circuit current after addition of glucose to the apical membrane. When examined under several conditions, neither insulin nor IGF-1 had an affect on the transport of glucose across the Caco-2 monolayer, nor the production of lactate by the cells. It is concluded that the insulin and IGF-1 receptors of Caco-2 cells do not regulate glucose transport.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 7","pages":"1093-113"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309063266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19351613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Receptor imaging by PET and SPECT: focus on the opiate receptor. PET和SPECT受体成像:聚焦于阿片受体。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073644

J J Frost

引用次数: 15

The Xenopus oocyte as an ectopic expression system for the selection of protein isoform-specific antibodies. 非洲爪蟾卵母细胞作为一种异位表达系统，用于选择蛋白异构体特异性抗体。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073676

S Reinhardt-Maelicke, S Kurz, S Sewing, M Stocker, O Pongs

引用次数: 1

Tissue plasminogen activator enhancing activity of vasopressin analogues in monkeys: structure-activity study. 组织纤溶酶原激活剂增强猴抗利尿激素类似物的活性:结构-活性研究。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073662

H Vilhardt, T Barth

引用次数: 0

Functional testing of human dopamine D1 and D5 receptors expressed in stable cAMP-responsive luciferase reporter cell lines. 稳定的camp反应性荧光素酶报告细胞系表达的人多巴胺D1和D5受体的功能检测。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073647

A Himmler, C Stratowa, A P Czernilofsky

{"title":"Functional testing of human dopamine D1 and D5 receptors expressed in stable cAMP-responsive luciferase reporter cell lines.","authors":"A Himmler, C Stratowa, A P Czernilofsky","doi":"10.3109/10799899309073647","DOIUrl":"https://doi.org/10.3109/10799899309073647","url":null,"abstract":"A large number of G-protein coupled receptors are known to modulate adenylyl cyclase activity. In order to find new compounds modulating the activity of specific receptor subtypes we developed a cellular screening system that measures the biological activity of drugs acting on receptors rather than merely their binding characteristics. The activity of the receptor coupling to the cAMP signal transduction pathway was measured via transcriptional activation of a reporter gene. A chinese hamster ovary cell line was stably transformed with a reporter plasmid containing the firefly luciferase gene under the transcriptional control of multiple cAMP responsive elements (CRE). This CRE reporter cell line exhibited 20 to 30-fold induction of luciferase activity upon stimulation of adenylyl cyclase with forskolin, but did not respond to dopamine agonists. Stable test cell lines were developed by transfecting reporter cell lines with human dopamine D1 and D5 receptor genes, respectively. Treatment of these test cell lines with dopamine receptor agonists and antagonists modulated the luciferase expression in a dose-dependent manner. The rank of potency of dopamine receptor agonists and antagonists was in agreement with reported data obtained from binding studies. The non-isotopic assay can be performed in microtiter plate format and is far less work intensive than the determination of adenylyl cyclase activity by direct cAMP measurement. This technology could also be utilized for discovery of new classes of compounds, e.g. allosteric effectors or non competitive ligands.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 1-4","pages":"79-94"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073647","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19369029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 109

Analysis of c-erb A RNA expression in the triiodothyronine-sensitive Ob 17 preadipocyte cell line. c- erba RNA在三碘甲状腺原氨酸敏感的ob17前脂肪细胞系中的表达分析。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073695

M Teboul, J Torresani

{"title":"Analysis of c-erb A RNA expression in the triiodothyronine-sensitive Ob 17 preadipocyte cell line.","authors":"M Teboul, J Torresani","doi":"10.3109/10799899309073695","DOIUrl":"https://doi.org/10.3109/10799899309073695","url":null,"abstract":"The protooncogenes erb A alpha and beta encode, in the rat, three functional thyroid hormone receptors (erb A alpha 1, beta 1 and beta 2), and two isoforms (erb A alpha 2, alpha 3) that do not bind triiodothyronine (T3). We previously reported on a mouse preadipocyte cell line (Ob 17) which was found to be sensitive to thyroid hormones and retinoic acid. Using antibodies or cDNA probes, we reported that the c-erb A products are mainly of the alpha-type in these cells. We also showed that the thyroid hormone receptors/c-erb A products are down-regulated moderately by T3 and strongly by retinoic acid added to the culture medium. In this work, different c-erb A subtypes are identified in this mouse cell line. Using couples of oligonucleotides known to be specific of each rat c-erb A subtype, we could detect the presence of alpha 1, alpha 2 and beta 1-type transcripts. We also detected Rev transcripts of a Reverse erb A alpha gene present in the rat at the c-erb A alpha locus and that contains a region complementary to the c-erb A alpha 2-specific region. These transcripts were identified by PCR amplification from cell cDNAs and in Northern analyses using the corresponding PCR-designed c-erb A probes. All the detected transcripts were found to be down-regulated by retinoic acid.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 5","pages":"815-28"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073695","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19370406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4