Journal of receptor research最新文献_第7页

Myasthenia gravis: effect on antibody binding of conservative substitutions of amino acid residues forming the main immunogenic region of the nicotinic acetylcholine receptor. 重症肌无力:烟碱乙酰胆碱受体主要免疫原区氨基酸残基保守取代对抗体结合的影响。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073698

J L Wahlsten, J M Lindstrom, N Ostlie, X D Wu, B M Conti-Tronconi

{"title":"Myasthenia gravis: effect on antibody binding of conservative substitutions of amino acid residues forming the main immunogenic region of the nicotinic acetylcholine receptor.","authors":"J L Wahlsten, J M Lindstrom, N Ostlie, X D Wu, B M Conti-Tronconi","doi":"10.3109/10799899309073698","DOIUrl":"https://doi.org/10.3109/10799899309073698","url":null,"abstract":"In Myasthenia Gravis most anti-acetylcholine receptor (AChR) antibodies are against a highly conserved area of the AChR alpha-subunit called the Main Immunogenic Region (MIR). Amino acid residues critical for MIR formation have been located within the sequence alpha 67-76. In the present study, binding of anti-AChR monoclonal antibodies (mAbs) to synthetic peptide analogues of the sequence alpha 67-76 of human and Torpedo AChRs containing conservative single-residue substitutions identified the amino acid residues most important to the antigenicity of the MIR sequence, and offered clues to its tridimensional structure. Conservative substitutions of residues Asn68 and Asp71 greatly diminished mAb binding, identifying them as critical contact residues for anti-MIR mAbs. Substitutions at Asp70 and Tyr72 moderately affected binding. Cross-reactive mAbs originally raised against Electrophorus AChR bound single residue-substituted synthetic peptides in a manner consistent with the possibility that Electrophorus AChR may have a glutamic acid residue at position alpha 70 or alpha 71. Substitutions at residues Asp/Ala70 and Val/Ile70 between human and Torpedo alpha-subunits may be size-compensating, suggesting these amino acids in the native AChR may be in closer proximity than proposed in previous models of the MIR.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 5","pages":"863-79"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19445972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Investigation on the potential role of the Ah receptor nuclear translocator protein in vitamin D receptor action. Ah受体核转运蛋白在维生素D受体作用中的潜在作用研究。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309063269

S Reisz-Porszasz, H Reyes, H F DeLuca, J M Prahl, O Hankinson

引用次数: 1

Binding of doxorubicin-conjugated transferrin to U937 cells. 阿霉素偶联转铁蛋白与U937细胞的结合。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309063263

V Szüts, A Bérczi, E Schweinzer, H Goldenberg

{"title":"Binding of doxorubicin-conjugated transferrin to U937 cells.","authors":"V Szüts, A Bérczi, E Schweinzer, H Goldenberg","doi":"10.3109/10799899309063263","DOIUrl":"https://doi.org/10.3109/10799899309063263","url":null,"abstract":"Binding of transferrin (Trf) and its doxorubicin-conjugated forms (Conj) to U937 cells at 0 degrees C were compared using 125I-labelled Trf or Conj. The apparent binding affinity (Ka) of Conj to the surface of U937 cells was (1.9 +/- 0.4).10(8) l/mol; it is about 40% of that of Trf [(5.0 +/- 1.2).10(8) l/mol]. Binding of 125I-labelled ligands was blocked by the unlabelled ligands to the same degree, however, it was not blocked by a great excess of doxorubicin (Dox). N-ethylmaleimide caused about 10% inhibition while dithiothreitol was without effect. Dissociation of 125I-labelled ligands in the presence of different concentrations of unlabelled ligands (Trf and Conj in the all 4 variations) resulted in different R50 values (the concentration of the unlabelled ligand where 50% of the radiolabelled ligand was released). While Trf displaced Trf with an R50 value close to the binding affinity, Conj displacement by Conj occurred with much lower efficiency. The heterolog displacement experiments yielded R50 values in between the two extrema. These results suggest that 1) binding of Conj to the surface of cells is governed by the binding of the Trf part of Conj to the transferrin receptor, 2) -SH groups are not involved in the binding, and 3) a second interaction between the Conj and some constituent(s) of the plasma membrane may modify the binding of Conj in comparison to that of Trf.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 7","pages":"1041-54"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309063263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19351611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

High level functional expression of human beta 1-adrenergic receptor in baculovirus-infected cells screened by a rapid in situ procedure. 快速原位筛选杆状病毒感染细胞中人β 1-肾上腺素能受体的高水平功能表达

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073678

V Ravet, N Blin, J L Guillaume, F Petitjean, L Cabanié, A D Strosberg

{"title":"High level functional expression of human beta 1-adrenergic receptor in baculovirus-infected cells screened by a rapid in situ procedure.","authors":"V Ravet, N Blin, J L Guillaume, F Petitjean, L Cabanié, A D Strosberg","doi":"10.3109/10799899309073678","DOIUrl":"https://doi.org/10.3109/10799899309073678","url":null,"abstract":"A novel screening assay for the identification of baculovirus infected cells expressing membrane receptors was developed by using a replica transfer technique. Sf9 cells were cotransfected with wild type baculoviral DNA and the transfer vector pVL941-beta 1 containing the coding region of the human beta 1-adrenergic receptor gene. Infected cells embedded in agarose were incubated with [125I]-iodocyanopindolol and transferred onto filters that were subsequently autoradiographed. This procedure resulted in the isolation of recombinant baculoviruses that expressed beta 1-adrenergic receptors. Binding assays carried out with [125I]-ICYP indicated that more than 600,000 receptors were expressed per cell, the highest level noted so far for this receptor in genetically engineered cells. Sf9 cells expressing the beta 1-AR were analysed by ligand binding, competition experiments, adenylyl cyclase stimulation and photoaffinity labeling. These cells express a homogenous population of receptors and display the known pharmacological properties of beta 1-AR in human tissues.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 1-4","pages":"541-58"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19369688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Functional domains of the Gs alpha subunit: role of the C-terminus in the receptor-dependent and receptor-independent activation. g α亚基的功能域:c端在受体依赖性和受体非依赖性激活中的作用。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073681

C Pantaloni, Y Audigier

引用次数: 4

New instruments for high throughput receptor binding assays. 高通量受体结合测定的新仪器。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073651

N Roessler, D Englert, K Neumann

引用次数: 8

A computer program for motion analysis of single cardiac myocytes. 用于单个心肌细胞运动分析的计算机程序。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073649

U T Rüegg, J F Zuber, R Best

{"title":"A computer program for motion analysis of single cardiac myocytes.","authors":"U T Rüegg, J F Zuber, R Best","doi":"10.3109/10799899309073649","DOIUrl":"https://doi.org/10.3109/10799899309073649","url":null,"abstract":"Single adult cardiac ventricular cells were prepared by collagenase perfusion of a rat heart. They were stimulated electrically in a perfusion chamber and their length changes were followed under a microscope. The motion was followed via a video camera and by a TV-line counting device and was recorded on-line by a personal computer. The program RECORD was used to calculate peak amplitude, base line drift and peak width at different peak heights allowing the determination of a number of variables of the cellular motion. The method was applied to drugs affecting the amplitude of contractions and the speed of relaxation. Results of beta-adrenergic stimulation, muscarinic inhibition and of the Ca(2+)-ATPase inhibitor cyclopiazonic acid (CPA) are shown. Besides its stimulatory effect on length, the beta-adrenergic agonist isoprenaline concentration-dependently shortened relaxation time. Carbachol reversed the increase in cellular shortening caused by isoprenaline in a concentration-dependent manner without fully reversing the shortened relaxation. CPA prolonged the return to diastole, presumably due to its inhibition of Ca(2+)-reuptake into the sarcoplasmatic reticulum.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 1-4","pages":"105-22"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19433776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Molecular cloning, stable expression and desensitization of the human dopamine D1b/D5 receptor. 人多巴胺D1b/D5受体的克隆、稳定表达及脱敏

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073680

K R Jarvie, M Tiberi, C Silvia, J A Gingrich, M G Caron

{"title":"Molecular cloning, stable expression and desensitization of the human dopamine D1b/D5 receptor.","authors":"K R Jarvie, M Tiberi, C Silvia, J A Gingrich, M G Caron","doi":"10.3109/10799899309073680","DOIUrl":"https://doi.org/10.3109/10799899309073680","url":null,"abstract":"The sub-family of dopamine D1-like receptors is now known to be comprised of at least two members: the originally cloned D1 receptor (herein referred to as the D1a receptor) and a related receptor referred to as the D1b, D1 beta or D5 dopamine receptor (herein referred to as the D1b/D5 receptor). Here, we characterize the D1b/D5 receptor expressed transiently in COS-7 cells and permanently in Ltk- cells. Transiently expressed human D1b/D5 receptors bind the D1 specific ligand [125I]SCH 23982 saturably and with high affinity (KD = 500 pM). Competition for [125I]SCH 23982 binding to rat D1b/D5 and human D1a and D1b/D5 receptors supports the contention that the two D1b/D5 receptors are species homologues. Furthermore, in COS-7 cells, as previously observed, dopamine competes for the binding of [125I]SCH 23982 to human D1b/D5 receptors with a higher affinity than that seen at the human D1a receptor. These results are similar to those seen in Ltk- cells permanently transfected with the human D1b/D5 receptor. In these cells, dopamine competition for [125I]SCH 23982 binding is complex, sensitive to guanine nucleotides and of a higher affinity than that observed for dopamine binding to the human D1a receptor expressed in these same cells. In both D1a and D1b/D5 expressing Ltk- cells, dopamine stimulates adenylyl cyclase with an EC50 of approximately 200 nM. Furthermore, preincubation of Ltk- cells expressing the D1a and D1b/D5 receptors with dopamine results in desensitization of the response of adenylyl cyclase to subsequent agonist stimulation.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 1-4","pages":"573-90"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073680","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19434351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Characterization of [3H]SK&F 108566 as a radioligand for angiotensin type-1 receptor. [3H]SK&F 108566作为血管紧张素1型受体放射配体的表征

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073697

N Aiyar, E Griffin, A Shu, R Heys, D J Bergsma, J Weinstock, R Edwards

{"title":"Characterization of [3H]SK&F 108566 as a radioligand for angiotensin type-1 receptor.","authors":"N Aiyar, E Griffin, A Shu, R Heys, D J Bergsma, J Weinstock, R Edwards","doi":"10.3109/10799899309073697","DOIUrl":"https://doi.org/10.3109/10799899309073697","url":null,"abstract":"Rat aortic smooth muscle cells were used as a model system to characterize the binding properties of [3H]SK&F 108566, an angiotensin type-1 (AT1) receptor antagonist. The binding was specific, saturable and reversible. The association and dissociation rates of [3H]SK&F 108566 binding to smooth muscle cells were monophasic and Scatchard analysis of equilibrium binding data yielded a linear plot indicating a homogenous population of binding sites. The maximum binding (Bmax) and apparent dissociation constant (Kd) were 22,000 +/- 6000 sites/cell and 0.83 +/- 0.08nM respectively. The pharmacological specificity of [3H]SK&F 108566 binding to smooth muscle cells is consistent with that observed for AT1 and confirms AT1 receptor specificity of this radioligand. High affinity binding was observed in membranes prepared from bovine adrenal cortex, rat liver and rat kidney glomeruli. COS cells transfected with cDNA encoding human AT1 angiotensin II receptors also displayed high affinity binding site for [3H]SK&F 108566. No specific binding could be detected on membranes prepared from bovine cerebellum, a tissue rich in the angiotensin type-2 (AT2) receptor. These observations indicate that [3H]SK&F 108566 binds to sites which have pharmacological characteristics of angiotensin II AT1 subtype receptors and can be used as a subtype-selective radioligand to characterize AII receptors in various systems.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 5","pages":"849-61"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19445971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Receptor sites for open channel blockers of Shaker voltage-gated potassium channels--molecular approaches. Shaker电压门控钾通道开放通道阻滞剂受体位点——分子途径。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073675

O Pongs

引用次数: 3