Journal of Plant Pathology最新文献_第9页

Species-specific real-time PCR assays for the detection of Venturia spp. on apple and pear, including the quarantine species V. nashicola 用于检测苹果和梨上卷曲病毒属（包括检疫物种 V. nashicola）的物种特异性实时 PCR 检测方法

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-22 DOI: 10.1007/s42161-024-01615-1

Elodie Muller, Jason Shiller, Bruno Le Cam, Yohana Laloum, Michel Girault, Renaud Ioos, Cécile Guinet

{"title":"Species-specific real-time PCR assays for the detection of Venturia spp. on apple and pear, including the quarantine species V. nashicola","authors":"Elodie Muller, Jason Shiller, Bruno Le Cam, Yohana Laloum, Michel Girault, Renaud Ioos, Cécile Guinet","doi":"10.1007/s42161-024-01615-1","DOIUrl":"https://doi.org/10.1007/s42161-024-01615-1","url":null,"abstract":"Several species of Venturia spp. cause scab disease on fruit trees: V. inaequalis on apple, V. pirina on pear, and V. nashicola on Asian pear that is listed as a quarantine pathogen in several countries in the world. An emerging disease caused by V. asperata on apple has very recently been reported in France, Italy and China. Fruit tree scab causes high economic losses and requires frequent fungicide treatments in orchards. Early detection of these pathogens is important in the management of this disease and—in the case of V. nashicola—to prevent its introduction and spread in disease-free areas. Using genomic resources available on these species, we identified polymorphic regions between them to develop a set of real-time PCR assays enabling detection of the four species on symptomatic fruits and leaves. We focused in particular on V. nashicola to establish a comprehensive validation procedure. The assay proved to be effective for targeting this quarantine species, thereby ensuring the reliability of analysis results in the context of regulatory monitoring.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"299 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139921861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal activity of zinc nanoparticles biosynthesized by Paenibacillus polymyxa D41 provides potent control against Fusarium culmorum in wheat 由多肉芽孢杆菌 D41 生物合成的纳米锌抗真菌活性可有效防治小麦中的镰刀菌

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-22 DOI: 10.1007/s42161-024-01607-1

{"title":"Antifungal activity of zinc nanoparticles biosynthesized by Paenibacillus polymyxa D41 provides potent control against Fusarium culmorum in wheat","authors":"","doi":"10.1007/s42161-024-01607-1","DOIUrl":"https://doi.org/10.1007/s42161-024-01607-1","url":null,"abstract":"<h3>Abstract</h3> Losses in cereal crops caused by Fusarium species are controlled by using chemical fungicides, which also adversely affect human health and the environment. Therefore, in this study, zinc nanoparticles (ZnNPs) with strong antifungal activity were biosynthesized by the bacterium Paenibacillus polymyxa and then used as a green fungicide to manage root rot disease in wheat. The ZnNPs were 44 nm, spherical, and had a net surface charge of − 28.65 mV and with the active coating, provided significant antioxidant and antifungal activity. The ZnNPs scavenged 89% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and at 40 µg/mL, inhibited growth of the pathogenic fungi Fusarium culmorum (FC), F. oxysporum, Candida albicans, and C. gelberta. In an in vivo experiment with FC-infected wheat, ZnNPs in water at 160 µg/mL significantly (p = 0.006) reduced preemergent root rot disease by 86% and significantly prevented postemergent disease (p = 0.001). As a result, incidence and severity of crown and root rot disease decreased by 79% and 89%, respectively. With ZnNPs, root weight remained similar to that in the control, but shoot weight decreased by 33%. Treatment with ZnNPs increased grain yield by 10% in healthy wheat and maintained it in FC-infected plants. Compared with control plants, the 1,000-grain weight increased by 40%. Total chlorophyll, carotenoids, and antioxidant contents were similar between FC-infected wheat and control plants. Thus, on the basis of the results, ZnNPs are recommended as a new green and safe fungicide.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"63 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139921808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil amendments for management of Phytophthora root rot in avocado and their impact on the soil microbiome 用于防治牛油果根腐病的土壤改良剂及其对土壤微生物组的影响

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-21 DOI: 10.1007/s42161-024-01604-4

Qurrat Ul Ain Farooq, Jen McComb, Giles E. St. J. Hardy, Treena I. Burgess

引用次数: 0

Growing our own poison–a vicious circle of more fungicides and more resistant Botrytis cinerea isolates 种植我们自己的毒药--更多杀菌剂和更多抗药性灰霉病菌分离物的恶性循环

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-20 DOI: 10.1007/s42161-023-01587-8

Jelenić Jelena, Ilić Jelena, Ćosić Jasenka, Vrandečić Karolina, Velki Mirna

{"title":"Growing our own poison–a vicious circle of more fungicides and more resistant Botrytis cinerea isolates","authors":"Jelenić Jelena, Ilić Jelena, Ćosić Jasenka, Vrandečić Karolina, Velki Mirna","doi":"10.1007/s42161-023-01587-8","DOIUrl":"https://doi.org/10.1007/s42161-023-01587-8","url":null,"abstract":"Grape production is seriously impacted by pests and diseases worldwide. Most producers rely heavily on the application of chemical pesticides to control pests and diseases of grapes and grapevines. With increasing rates of fungicide use, active ingredients may decrease in efficacy or become inefficacious due to the emergence of resistance in the organism targeted by the treatment. This research was conducted with the aim of assessing the sensitivity of Botrytis cinerea to five fungicide formulations (active ingredients: boscalid, cyprodinil + fludioxonil, fenpyrazamine, fenhexamid, and pyrimethanil), four of which have been registered and used for more than 10 years in Croatia. Even at the highest concentrations tested, pyrimethanil, boscalid and fenhexamid caused fungal inhibition at a rate significantly lower than 90%. Conversely, cyprodinil + fludioxonil had inhibition rates greater than 90% for 72% of the isolates. Finally, the fungicide fenpyrazamine, which is not registered for use in Croatian viticulture, resulted in fungal inhibition rates of less than 40%. To our knowledge, this is one of the few studies conducted in the Slavonia region of Croatia on the potential emergence of resistance to chemically active ingredients in B. cinerea populations. The results of the present study show that B. cinerea resistance to several active ingredients is of significant concern due to the small number of registered products available in Croatia to combat the disease this fungus causes on grapes.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"72 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139910902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of tomato spotted wilt virus by real-time RT-LAMP and in-field application 通过实时 RT-LAMP 和田间应用快速检测番茄斑萎病毒

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-19 DOI: 10.1007/s42161-024-01613-3

A.G. Caruso, A. Ragona, G. Agrò, S. Bertacca, E. Yahyaoui, L. Galipienso, L. Rubio, S. Panno, S. Davino

{"title":"Rapid detection of tomato spotted wilt virus by real-time RT-LAMP and in-field application","authors":"A.G. Caruso, A. Ragona, G. Agrò, S. Bertacca, E. Yahyaoui, L. Galipienso, L. Rubio, S. Panno, S. Davino","doi":"10.1007/s42161-024-01613-3","DOIUrl":"https://doi.org/10.1007/s42161-024-01613-3","url":null,"abstract":"Tomato spotted wilt virus (TSWV) is considered one of the most threatening viruses worldwide for different economically important agricultural crops. In this scenario, it is important to perform an early detection by laboratory tests to prevent TSWV spread. A rapid and sensitive TSWV detection protocol based on real time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed in this work, also using cost-effective and simplified sample preparation procedure, to assess the suitability of the RT-LAMP assay in field conditions on tomato and pepper samples. A set of six primers was designed within the nucleotide sequence region coding for the nucleocapsid protein (N) of segment S, targeting a 220-nucleotide sequence. Sensitivity, specificity, accuracy, and in-field application of the real-time RT-LAMP assay were evaluated. The developed real-time RT-LAMP assay proved to be one thousand and one hundred times more sensitive than end-point RT-PCR and real-time RT-PCR methods, respectively, detecting a total of 9.191 × 101 genome copies as minimum target, and no cross-reactivity were detected with other viruses belonging to Tospoviridae and Bromoviridae families used as outgroup. In addition, the in-field application of the assay using the rapid sample preparation gave adequate and reliable results within 60 minutes, with an acceptable reaction delay when compared to canonical RNA extraction. The in-field analyses showed an increase of TSWV-positive samples (37%) detection compared with end-point RT-PCR and real-time RT-PCR (32% and 29%, respectively), particularly on asymptomatic samples, confirming that the real-time RT-LAMP assay can be implemented as a routine test both in-field and laboratory conditions as a rapid and sensitive technique for TSWV detection.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"210 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139901997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burning questions for fire blight research: I. Genomics and evolution of Erwinia amylovora and analyses of host-pathogen interactions 火疫病研究的迫切问题：I. Erwinia amylovora 的基因组学和进化以及宿主-病原体相互作用分析

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-14 DOI: 10.1007/s42161-023-01581-0

Fabio Rezzonico, Ofere Francis Emeriewen, Quan Zeng, Andreas Peil, Theo H.M. Smits, George W. Sundin

引用次数: 0

Evaluation of antagonistic mechanisms of bacterial species for their biocontrol activity against fire blight (Erwinia amylovora) 评估细菌物种的拮抗机制，以确定其对火疫病（Erwinia amylovora）的生物防治活性

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-12 DOI: 10.1007/s42161-024-01601-7

Artur Mikiciński, Joanna Puławska, Assel Molzhigitova, Piotr Sobiczewski

{"title":"Evaluation of antagonistic mechanisms of bacterial species for their biocontrol activity against fire blight (Erwinia amylovora)","authors":"Artur Mikiciński, Joanna Puławska, Assel Molzhigitova, Piotr Sobiczewski","doi":"10.1007/s42161-024-01601-7","DOIUrl":"https://doi.org/10.1007/s42161-024-01601-7","url":null,"abstract":"The paper presents an attempt to explain the potential mechanisms related to the biocontrol capacity of the four strains representing bacterial species in which this activity was observed for the first time (Pseudomonas vancouverensis L16, Pseudomonas chlororaphis subsp. aureofaciens 3M, Enterobacter ludwigii 43M and Pseudomonas protegens 59M). The P. protegens 59M strain showed the highest effectiveness in protecting pear fruitlets against fire blight. The phenotypic analyses of tested mechanisms showed that all strains demonstrated the ability of motility, hydrogen cyanide production and degradation of nicotinic acid. Also, all strains except E. ludwigii 43M, produced siderophores and P. vancouverensis L16 was able to produce biosurfactant, salicylic acid and indole-3-acetic acid (IAA) while the E. ludwigii 43M and P. chlororaphis subsp. aureofaciens 3M strains produced IAA and homoserine lactones (AHL), respectively. A study on the detection of genes encoding antibiotics characteristic of pseudomonads showed the presence of prnD in the P. chlororaphis subsp. aureofaciens 3M strain and phlD, pltC, pltB and pltC in P. protegens 59M, which suggested that the latter strain had the largest number of antibiotic-coding genes among all the strains tested. The regulatory gene gacA was also present in P. protegens 59M and P. chlororaphis subsp. aureofaciens 3M strains. However, none of the genes sought were detected in the L16 strain. It was assumed that the ability of strain P. protegens 59M to completely protect slices of pear fruitlet against E. amylovora infection is related to the production of antibiotics and possibly also to the other detected mechanisms of antagonism, such as siderophore, IAA and HCN production.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"79 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139770032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of boron and mefenoxam on growth of the isolates of Phytophthora cinnamomi obtained from avocado orchards 硼和甲拌磷对从鳄梨果园获得的 Phytophthora cinnamomi 分离物生长的影响

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-12 DOI: 10.1007/s42161-024-01598-z

İlker Kurbetli

{"title":"Effects of boron and mefenoxam on growth of the isolates of Phytophthora cinnamomi obtained from avocado orchards","authors":"İlker Kurbetli","doi":"10.1007/s42161-024-01598-z","DOIUrl":"https://doi.org/10.1007/s42161-024-01598-z","url":null,"abstract":"Avocado root rot caused by Phytophthora cinnamomi is a major disease of the crop, management of which can be accomplished, partially, by reducing the amount of the pathogen inoculum in the soil. Boron is known to have fungicidal properties and has been used in controlling plant diseases. Although boron at a concentration of 1 μg mL−1 led to increased mycelial growth of P. cinnamomi, increasing the dose rate to 10 μg mL−1 decreased colony diameter; no mycelial growth occurred at 1000 μg mL−1. In the absence of inoculation with P. cinnamomi or treatment with boron (negative controls), avocado seeds germinated and emerged healthy, whereas no germination occurred in seeds of the positive control (inoculated with P. cinnamomi; no boron) treatment, or seeds inoculated with P. cinnamomi and treated with different concentrations of boron. Seeds treated with 500 μg mL−1 boron were more severely infected with P. cinnamomi than the positive controls, but seed infectivity of the pathogen decreased with increasing dose rates of boron. Although little fresh roots growth was observed even in the roots of the positive control seedlings (inoculated with P. cinnamomi and not treated with boron), almost no fresh roots formed on boron-treated plants, while negative control seedlings (non-inoculated with P. cinnamomi, not treated with boron) formed abundant fresh roots. However, as the applied boron dose increased, there was a decrease in the severity of root crown rot of the seedlings. All P. cinnamomi isolates were sensitive to mefenoxam with EC50 values between 0.002 and 0.3 μg mL−1.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"185 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139770039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unleashing the potential of multitrait onion seed endophytic bacteria in combating purple blotch incited by Alternaria porri (Ellis) Cif 挖掘洋葱种子内生细菌的潜力，防治由多孔菌（Alternaria porri (Ellis) Cif）引发的紫斑病

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-12 DOI: 10.1007/s42161-024-01594-3

Shubham Saini, Kushal Raj, Leela Wati, Rakesh Kumar, Anil Kumar Saini, Mukul Kumar Bhambhu, Makhan Lal

{"title":"Unleashing the potential of multitrait onion seed endophytic bacteria in combating purple blotch incited by Alternaria porri (Ellis) Cif","authors":"Shubham Saini, Kushal Raj, Leela Wati, Rakesh Kumar, Anil Kumar Saini, Mukul Kumar Bhambhu, Makhan Lal","doi":"10.1007/s42161-024-01594-3","DOIUrl":"https://doi.org/10.1007/s42161-024-01594-3","url":null,"abstract":"Onion purple blotch incited by Alternaria porri is a serious bottleneck in sound cultivation of crop. The management of disease through the use of fungicides is matter of concern owing their indispensable impact on environment. A paradigm shift toward use of eco-friendly management tactics involving the use of seed endophytes presents new frontiers in research. The endophytic bacteria retrieved from onion seeds (Hisar Onion 2) presented diverse range of biocontrol and plant growth promoting traits. Dual culture assay revealed pre-eminence of OSEB 11 (72.35 per cent) and OSEB 3 (67.06 per cent) in supressing mycelial growth of Alternaria porri. Ten bacterial isolates exhibited ammonia excretion, seven isolates were recorded positive for siderophore production, four isolates presented cyanogenic potential while five isolates exhibited cellulase activity. OSEB 1, OSEB 12 and OSEB 14 were found positive for all the performed biocontrol and plant growth promoting traits. The MALDI TOF based characterization of promising endophytic bacteria revealed identity of OSEB 3 as Enterobacter cloacae and that of OSEB 11 as Stenotrophomonas maltophila. In planta evaluation of promising endophytes indicated that foliar spray of Stenotrophomonas maltophila was highly influential in enhancing phenolics and antioxidant enzymes (peroxidase, polyphenol oxidase and phenylalanine ammonia lyase) in onion with consequent suppression of purple blotch disease, leading to 41.80 per cent management over control. Field evaluation of promising endophytes also revealed efficacy of foliar spray of Stenotrophomonas maltophila in supressing the disease (33.85 per cent disease management over control) and enhancing seed yield.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"6 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139769946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of susceptibility of commercial citrus rootstocks to white root rot incited by Rosellinia necatrix 评估商用柑橘砧木对白根腐病（Rosellinia necatrix）的敏感性

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-02-12 DOI: 10.1007/s42161-024-01599-y

Juan M. Arjona-López, José A. Monferrer-Salinas, José Luís Cantero-Sánchez, Estefanía Romero-Rodríguez, Carlos J. López-Herrera, Francisco J. Arenas-Arenas

{"title":"Evaluation of susceptibility of commercial citrus rootstocks to white root rot incited by Rosellinia necatrix","authors":"Juan M. Arjona-López, José A. Monferrer-Salinas, José Luís Cantero-Sánchez, Estefanía Romero-Rodríguez, Carlos J. López-Herrera, Francisco J. Arenas-Arenas","doi":"10.1007/s42161-024-01599-y","DOIUrl":"https://doi.org/10.1007/s42161-024-01599-y","url":null,"abstract":"Spain reached the fifth position of citrus production and the first of fresh fruit exporter worldwide. Pre-harvest diseases caused by soil-borne pathogens can limit citrus crop production. Phytophthora spp. are considered the most serious soil-borne pathogens of citrus crops worldwide, but their management strategies are highly developed. Conversely, Rosellinia necatrix is another soil-borne pathogen with polyphagous behavior, including citrus trees, and without fully-effective control tools. In this sense, the aim of this study was to characterize the susceptibility of several commercial citrus rootstocks to R. necatrix disease. Potted plants from 10 different rootstocks were artificial inoculated with an isolate of R. necatrix. Plant physiological measurements of above-ground symptoms, chlorophyll content, biomass and leaf area were obtained. Flying Dragon and Forner-Alcaide 517 citrus rootstocks displayed the lowest disease incidence of SAUDPC, whereas Citrus macrophylla the highest symptoms rate. The highest chlorophyll content was found in Forner-Alcaide 517, and the lowest in Citrus macrophylla and Bitters C-22. Additionally, this last rootstock reached the highest reduction of biomass and the lowest leaf area rate. In conclusion, Forner-Alcaide 517 could be an interesting choice for those orchards with R. necatrix problems; contrarily, Citrus macrophylla and Bitters C-22 were the most sensitive candidates to this pathogen.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"11 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139770037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0