Journal of Pharmaceutical and Biopharmaceutical Research最新文献_第4页

Microbial synthesis of anti-tumor agent oxysophoridine through one step by filamentous fungus 丝状真菌一步法合成抗肿瘤药物氧化硫定

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-05-09 DOI: 10.25082/JPBR.2019.02.001

Qing-feng Meng, Xuan Zhou, Xu Ran, Song Yan, Shaobin Fu

{"title":"Microbial synthesis of anti-tumor agent oxysophoridine through one step by filamentous fungus","authors":"Qing-feng Meng, Xuan Zhou, Xu Ran, Song Yan, Shaobin Fu","doi":"10.25082/JPBR.2019.02.001","DOIUrl":"https://doi.org/10.25082/JPBR.2019.02.001","url":null,"abstract":"Sophoridine and oxysophoridine are alkaloids extracted from Chinese traditional medicine with significant antitumor activities. Computer-aided design and preliminary structure-activity relationship studies showed that the biological activity of sophoridine can be improved after oxidation (oxysophoridine) while its toxicity significantly decreased. A new method to prepare oxysophoridine from natural product Sophoridine by biotransformation was established for the first time except for traditional extraction from plants and chemical synthesis. Positive biocatalytic reaction was detected by TLC. Structure of conversion product was elucidated based on NMR spectroscopy. Optimizations of reactions including substrate concentration, pH, conversion time and inoculation quantity were complemented which analyzed by LC-MS. Results showed that filamentous fungus Xylariales. sp F005 (CCTCC M2014660) has the capacity of synthetizing oxysophoridine from sophoridine in the selected 37 strains. The yield of oxysophoridine reached highest at conditions pH 6.0, the third day of conversion time, 5.0 mg substrate and 5% inoculums respectively. The method for preparing oxysophoridine catalyzed by enzymes with green and sustainable synthetic processes was developed.","PeriodicalId":16703,"journal":{"name":"Journal of Pharmaceutical and Biopharmaceutical Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86766748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

SeDeM Expert System：A review and new perspectives SeDeM专家系统:综述与新观点

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-04-24 DOI: 10.25082/jpbr.2019.01.005

Huimin Sun, Mingxian Gu, Tianbing Guan, Shulin Wan, Han Zhang, Xuelian Li, S. Kong, Jianbing Ren, Chuanyun Dai

引用次数: 5

Spectroscopic study on the mechanism of meloxicam and α-amylase 美洛昔康与α-淀粉酶作用机理的光谱研究

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-04-17 DOI: 10.25082/JPBR.2019.01.004

Xu Cheng, Bao-Sheng Liu, Hongcai Zhang

{"title":"Spectroscopic study on the mechanism of meloxicam and α-amylase","authors":"Xu Cheng, Bao-Sheng Liu, Hongcai Zhang","doi":"10.25082/JPBR.2019.01.004","DOIUrl":"https://doi.org/10.25082/JPBR.2019.01.004","url":null,"abstract":"In order to explore the mechanism of action of meloxicam and α-amylase. The interaction between the rheumatoid arthritis drug meloxicam and α-amylase was studied by fluorescence spectroscopy, synchronous fluorescence spectroscopy and molecular docking under the experimental conditions of pH=6.80. The results showed that meloxicam was able to effectively quench the endogenous fluorescence of α-amylase in a static quenching form a 1:1 complex and change the conformation of α-amylase. Thermodynamic results indicated that the main type of meloxicam and α-amylase system was hydrophobic interaction. Molecular docking indicated that the binding system had hydrogen bonds in addition to hydrophobic interaction and meloxicam was surrounded by the active amino acid residues Trp13 and Trp263 of α-amylase, which changed the microenvironment of amino acid residues at the active center of α-amylase. By establishing the binding model, it can be seen that the protein binding rate W(B) of meloxicam to α-amylase was 2.76%-41.79% under the experimental conditions. The results showed that the binding of meloxicam to α-amylase had an effect on the number of free α-amylase. The drug binding rate W(Q) of the system was 2.76%-1.67%, which indicated that the combination of α-amylase and meloxicam would not affect the efficacy of meloxicam.","PeriodicalId":16703,"journal":{"name":"Journal of Pharmaceutical and Biopharmaceutical Research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78796628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binding studies of trans-resveratrol with superoxide dismutase (SOD1): Docking assessment and Thermoanalysis 反式白藜芦醇与超氧化物歧化酶(SOD1)的结合研究:对接评估和热分析

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-02-22 DOI: 10.25082/JPBR.2019.01.003

Janhvi Dureja, R. Chadha, M. Karan, Akshita Jindal, K. Chadha

{"title":"Binding studies of trans-resveratrol with superoxide dismutase (SOD1): Docking assessment and Thermoanalysis","authors":"Janhvi Dureja, R. Chadha, M. Karan, Akshita Jindal, K. Chadha","doi":"10.25082/JPBR.2019.01.003","DOIUrl":"https://doi.org/10.25082/JPBR.2019.01.003","url":null,"abstract":"The binding pursuits of trans-resveratrol (t-RSV), an amazing health supplement are investigated with an antioxidant enzyme, superoxide dismutase (SOD1). The aim of the study is to dock t-RSV on the adrenaline binding site on SOD1 in order to explore its potential to act as a safety net against amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disorder that affects motor neurons. In silico GLIDE docking methodology and in vitro microcalorimetry technique is utilized for the investigation of binding parameters of t-RSV with SOD1. The study provides useful and distinct information about the amino acids involved in the interactions at molecular level along with the nature of forces involved in binding of t-RSV with SOD1. The docking analysis using the scoring functions of Schrodinger's Glide package depicts that GLU100, PRO28, LYS23, TRP32 residues of the peptide backbone on SOD 1 interact with phenolic groups of t-RSV. The information on thermodynamic parameters, i.e. binding constant (Kb), free energy (ΔG) and enthalpy (ΔH) generated through calorimetric titrations suggests that the reaction between t-RSV and SOD 1 is spontaneous and exothermic. Both the studies are found to be in close agreement with each other based as far as the magnitude of binding constant (Kb =9.9 x10 4) is concerned.","PeriodicalId":16703,"journal":{"name":"Journal of Pharmaceutical and Biopharmaceutical Research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75993212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The preparation of Garcinia Glycosides solid dispersion and intestinal absorption by rat in situ single pass intestinal perfusion 大鼠原位单次肠灌注制备藤黄苷固体分散体及肠吸收

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-01-24 DOI: 10.25082/JPBR.2019.01.002

Ye-jun Chen, L. Shengnan, Ji Jingchao, Cheng Yinghui, Ju Liu, Yang Wang, Hongsheng Liu

引用次数: 0

Functionalization of carbopol with NVP for designing antibiotic drug loaded hydrogel dressings for better wound management 卡泊泊与NVP功能化设计抗生素药物负载水凝胶敷料，以改善伤口管理

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-01-22 DOI: 10.25082/JPBR.2019.01.001

Baljit Singh, A. Dhiman

引用次数: 11

Pharmacy, the interdisciplinary field of science 药学，跨学科的科学领域

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-01-14 DOI: 10.25082/JPBR.2018.01.001

P. Perjési

引用次数: 1

Synthesis of cocrystals of sulfonyl urea class drug using suitable coformers for enhancement of aqueous solubility 磺酰脲类药物的共晶合成，采用合适的共构象增强其水溶性

Journal of Pharmaceutical and Biopharmaceutical Research Pub Date : 2019-01-01 DOI: 10.25082/jpbr.2019.02.003

Santosh S. Chhajed, Yatish R. Rajderkar, Anjali B. Tajanpure, Jayprakash B. Ugale, Sanjay J. Kshirsagar

引用次数: 1