Journal of pharmaceutical and biomedical analysis最新文献_第6页

Novel approaches in CRISPR/Cas12a-based sensing for HCC diagnosis - A review (2020–2025) 基于CRISPR/ cas12的HCC诊断传感新方法综述（2020-2025）

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-07 DOI: 10.1016/j.jpba.2025.116878

Cunhong Cen , Xiyu Liu , Wei He , Xiaohong Tan , Guiyin Li , Na Jintong

引用次数: 0

Pseudotargeted metabolomics profiles potential damage-associated molecular patterns as machine learning predictors for acute pancreatitis 伪靶向代谢组学描述了潜在的损伤相关分子模式，作为急性胰腺炎的机器学习预测因子

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-07 DOI: 10.1016/j.jpba.2025.116874

Wen-Lu Cai , Can Fang , Hong-Xu Leng , Jia-Yi Zheng , Li-Fang Liu , Guan-Wen Gong , Gui-Zhong Xin

{"title":"Pseudotargeted metabolomics profiles potential damage-associated molecular patterns as machine learning predictors for acute pancreatitis","authors":"Wen-Lu Cai , Can Fang , Hong-Xu Leng , Jia-Yi Zheng , Li-Fang Liu , Guan-Wen Gong , Gui-Zhong Xin","doi":"10.1016/j.jpba.2025.116874","DOIUrl":"10.1016/j.jpba.2025.116874","url":null,"abstract":"<div><div>Acute pancreatitis (AP) is a common gastrointestinal disease characterized by pancreatic cell damage and inflammation. Given the early clinical diagnosis and management challenges, exploring novel analytical frameworks from new orientations for interrogating AP is urgent. The release of damage-associated molecular patterns (DAMPs) and their receptor recognition initiate sterile inflammation, serving as key drivers in the development and progression of AP. Thus, this study aimed to delineate the underlying correlations between alterations in the DAMP profile and the AP state. We have developed a new framework combining potential DAMPs profiles obtained from pseudotargeted metabolomics method with machine learning (ML) models for AP prediction. 2-(1-Piperazinyl) pyrimidine chemical labeling was utilized to provide characteristic fragment ions and improve the quantitative sensitivity of targeted metabolites. A total of 49 potential DAMPs were identified and semi-quantified from collected serum samples (n = 84), positive or negative for APs. For modeling obtained datasets with five different ML algorithms, the support vector machine model was chosen as the optimal model to differentiate with high accuracy, achieving an area under the receiver-operating characteristic curve (AUROC) of 0.944. It also showed a strong performance in an external independent validation set (AUROC: 0.907). Moreover, the model was interpreted using the Shapley Additive exPlanations analysis to specify the important features and identify specific free fatty acids as key contributors. Overall, the novel framework enables high accuracy in predicting the presence of AP status. Meanwhile, it underlines the utility of DAMPs in inflammatory diseases and provides reference values for diagnosing in first-line clinics.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116874"},"PeriodicalIF":3.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alcohol-based solvents as mobile phases for LC-MS characterization of therapeutic proteins 醇基溶剂作为流动相用于治疗性蛋白的LC-MS表征

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-07 DOI: 10.1016/j.jpba.2025.116879

Mostafa Zarei , Jérôme Jonveaux, Michael Jahn

{"title":"Alcohol-based solvents as mobile phases for LC-MS characterization of therapeutic proteins","authors":"Mostafa Zarei , Jérôme Jonveaux, Michael Jahn","doi":"10.1016/j.jpba.2025.116879","DOIUrl":"10.1016/j.jpba.2025.116879","url":null,"abstract":"<div><div>Acetonitrile (ACN) is currently the preferred solvent in reversed phase (RP) chromatography for protein characterization through peptide mapping. Despite its effective performance, ACN poses toxicity risks to humans and has adverse effects on environmental sustainability. In the current work, we developed a novel alcohol-based solvent system for peptide mapping by systematic evaluation of parameters such as organic eluent composition, solvent gradient, flow rate, and column temperature. We compared the chromatographic performance and MS response of peptides between the standard (ACN based) and the new developed solvent systems (EtOH/IPA based). The results of our study show that the EtOH/IPA based solvent system improves selectivity factor (α) and resolution (R), while the standard ACN based solvent system provides a lower peak width and hence a higher peak height. The majority of the analysed peptides exhibited shorter retention times, whereas hydrophobic peptides eluted later when using the EtOH/IPA solvent system. Several critical quality attributes (CQA) of a monoclonal antibody (mAb) were successfully characterized by this method without compromising the chromatographic separations and MS response of the peptides. Furthermore, the suitability of the new approach for LC-UV assessment of a mAb as part of an identity test of therapeutic proteins was demonstrated. Our proposed approach, which prioritizes safety, non-toxicity, and compatibility with all LC-MS instruments, offers significant support to a broad community of academic and biopharmaceutical scientists in their pursuit of a bottom-up green strategy.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116879"},"PeriodicalIF":3.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode 掺杂硼金刚石电极上新型大麻素分离电化学检测的高效液相色谱方法

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-07 DOI: 10.1016/j.jpba.2025.116875

Zeynab Belbasi , Jan Petr , Jan Hrbac , David Jirovsky

{"title":"HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode","authors":"Zeynab Belbasi , Jan Petr , Jan Hrbac , David Jirovsky","doi":"10.1016/j.jpba.2025.116875","DOIUrl":"10.1016/j.jpba.2025.116875","url":null,"abstract":"<div><div>In this study, we developed a novel and environmentally friendly method for the determination of selected cannabinoids — cannabidiol, cannabinol, tetrahydrocannabinol, JWH 073 synthetic cannabinoid, hexahydrocannabinol (HHC), and hexahydrocannabinol-O-acetate — using high-performance liquid chromatography with electrochemical detection. The method employs a boron-doped diamond working electrode, which offers significant advantages in comparison with commonly used materials. Its main strengths include low background currents, reduced fouling, and an extended potential window, making it suitable for detecting compounds that are not readily oxidizable. The chromatographic system utilized a reversed-phase Gemini C18 column with a mobile phase consisting of acetonitrile and 25 mM phosphate buffer (pH 4.4) in step-gradient mode from 68 % to 88 % acetonitrile. The separation and determination of cannabinoids were optimized to be completed in less than 25 min. Hydrodynamic voltammetry was performed to identify the optimal potential for the amperometric detection, with a working potential of + 1400 mV (vs. Pd/H₂) providing the best analytical response based on S/N ratio. Under these optimal separation and detection conditions, the limits of detection were within the range of 6.1–666 nM (for cannabidiol and 9(R)-hexahydrocannabinol-O-acetate), while the response was linear within the concentration range measured up to 10 μM. The method demonstrated good linearity, precision, and sensitivity, with limits of detection and quantitation reaching nanomolar levels. The developed method was successfully applied to commercial samples of HHC jelly bears and HHC distillate.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116875"},"PeriodicalIF":3.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intervention study of Yanghe decoction on plasma cell mastitis based on mammary microecology and metabolomics investigation 基于乳腺微生态学和代谢组学研究的洋河煎剂对浆细胞性乳腺炎的干预研究

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-05 DOI: 10.1016/j.jpba.2025.116870

Feixia Ma , Yi Xiao , Luyao Qian , Shuo Zhang

{"title":"Intervention study of Yanghe decoction on plasma cell mastitis based on mammary microecology and metabolomics investigation","authors":"Feixia Ma , Yi Xiao , Luyao Qian , Shuo Zhang","doi":"10.1016/j.jpba.2025.116870","DOIUrl":"10.1016/j.jpba.2025.116870","url":null,"abstract":"<div><div>Plasma cell mastitis (PCM), a chronic breast inflammatory disease, is characterized by ductal dilatation and plasma cell infiltration. Traditional Chinese Medicine (TCM) Yanghe decoction is used to relieve mastitis symptoms clinically. However, its efficacy and mechanism on PCM remain undefined. The PCM patients receiving dexamethasone and metronidazole tablets, served as control group, with the other group receiving combination of Yanghe decoction, 15 cases each. Main symptom indicators included mass size, skin colour, skin temperature, breast pain, blood cell count (WBC), C reactive protein (CRP), interleukin (IL)-6, IL-4, serum prolactin (PRL), erythrocyte sedimentation rate (ESR), immunoglobulin A (IgA), IgM, and IgG levels. Secondary indicators included TCM syndrome and anxiety/depression scores. Breast differential flora (DF) and metabolites (DAMs) post-treatment between two groups were detected by 16S rRNA sequencing and metabolomics, with further correlation analysis. Post-treatment symptom scores exhibited lower than pre-treatment in both groups, with greater decline on WBC, CRP, IL-6, IL-4, PRL, and ESR, and enhanced IgA, IgM, and IgG levels in Yanghe decoction group than in control group. Notably, Yanghe decoction group demonstrated no significant DF, with decreased <em>Corynebacterium</em> and <em>Rhodococcus,</em> and elevated <em>Staphylococcus</em>, which correlated significantly with the above indicators. Moreover, 43 DAMs were detected between these two groups, with Glycerophosphocholine, 9,10-Epoxyoctadecenoic acid, Arachidic acid, and 4,5-Dihydroorotic acid showing strong correlations with the above flora. Based on the clarification of PCM improvement with Yanghe decoction, we preliminarily explored potential roles of breast tissue microorganisms and DAMs, providing scientific basis for its clinical application and potential clinical biomarkers in PCM.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116870"},"PeriodicalIF":3.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the hepatoprotective effects of Bicyclol's forced degradation products using a zebrafish model 利用斑马鱼模型评估双环醇强制降解产物的肝保护作用

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-04 DOI: 10.1016/j.jpba.2025.116871

Mengqi Jia , Runjuan Yang , Cui Ma , Chao Gu , Dongying Wu , Xucong Huang , Lili Jing , Guorong Fan

{"title":"Assessment of the hepatoprotective effects of Bicyclol's forced degradation products using a zebrafish model","authors":"Mengqi Jia , Runjuan Yang , Cui Ma , Chao Gu , Dongying Wu , Xucong Huang , Lili Jing , Guorong Fan","doi":"10.1016/j.jpba.2025.116871","DOIUrl":"10.1016/j.jpba.2025.116871","url":null,"abstract":"<div><div>Bicyclol (BIC), a synthetic hepatoprotective agent widely prescribed in China, lacks comprehensive safety and activity profiles for its degradation products (DPs). Here, we systematically investigated BIC’s forced degradation behavior using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Three hydrolytic degradation products (DP1-DP3) were isolated via HPLC and structurally characterized, revealing methylenedioxy group hydrolysis as the primary degradation pathway. Molecular docking simulations demonstrated enhanced target binding affinities of DPs compared to BIC, supported by absorption, distribution, metabolism, and excretion (ADME) predictions showing improved drug-likeness. In an alcohol-induced fatty liver zebrafish model, both BIC and its DPs attenuated hepatic macrovesicular steatosis and inflammatory responses. Mechanistically, treatment normalized lipid metabolism by downregulating alcohol-induced expression of <em>FASN</em>, <em>SREBP1</em>, <em>PPARα</em>, and <em>PPARγ</em>, while reduced IL-6 and TNF-α levels confirmed anti-inflammatory efficacy. These findings demonstrate that BIC DPs exhibit dual pharmacological activity through lipid homeostasis modulation and inflammation suppression, providing critical insights for quality control and therapeutic applications.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116871"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated cartilage metabolomics and proteomics analysis reveals the therapeutic effect of Wenjing Tongluo Decoction on Knee osteoarthritis rats 综合软骨代谢组学和蛋白质组学分析揭示文静通络汤对膝关节骨性关节炎大鼠的治疗作用

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-04 DOI: 10.1016/j.jpba.2025.116869

Wei Wei , Chenjian Peng , Renjun Gu , Xiwu Yan , Jiapeng Ye , An Kang , Luning Sun

{"title":"Integrated cartilage metabolomics and proteomics analysis reveals the therapeutic effect of Wenjing Tongluo Decoction on Knee osteoarthritis rats","authors":"Wei Wei , Chenjian Peng , Renjun Gu , Xiwu Yan , Jiapeng Ye , An Kang , Luning Sun","doi":"10.1016/j.jpba.2025.116869","DOIUrl":"10.1016/j.jpba.2025.116869","url":null,"abstract":"<div><div>Knee osteoarthritis (KOA)is an age-related degenerative whole-joint disease characterized by poor outcomes. Wenjing Tongluo Decoction (WJTLD), a Chinese herbal remedy, has demonstrated favorable clinical effects on KOA. However, the precise mechanisms therein remain poorly defined. In this study, we employed the method of anterior cruciate ligament transection (ACLT) method to establish a rat model of KOA. Following 8 weeks of oral administration of WJTLD, the morphology of knee joint cartilage was evaluated using Safranin-O/Fast green staining, H&E staining, and micro-CT imaging. Utilizing GC-MS based untargeted metabolomics and nano-LC-QE-MS based proteomics, we identified altered metabolites and proteins associated with knee cartilage in different rat groups, which were further validated through western blotting and real-time PCR. Our findings indicate that WJTLD alleviates damage to knee joint cartilage and inhibits cartilage degradation. Proteomics data revealed that the altered proteins in OA and WJTLD treated group were related to the biological process including amoebiasis, platelet activation, ECM-receptor interaction, protein digestion and absorption, and ribosome function. Western blotting results confirmed that the expression levels of MMP8 and LDHA were significantly upregulated in the KOA group but were rescued by WJTLD treatment. According to untargeted metabolomics, the intensities of lactic acid, isoleucine, lysine, glutamate, myo-inositol, adenosine, and β-alanine were significantly elevated in the KOA group, however, these metabolites experienced a dramatic following WJTLD treatment. These results suggest that WJTLD exerts a therapeutic effect on KOA by suppressing inflammation and cartilage degradation, as well as regulating multiple pathways related to ECM degradation, amino acid metabolism, and energy metabolism, including glycolysis.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116869"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fourier transform infrared spectroscopy, high-performance liquid chromatography with diode array detection, and gas chromatography-mass spectrometry fingerprints combined with chemometrics for comprehensive evaluation and identification of raw and bran-fried Atractylodis Rhizoma 采用傅里叶变换红外光谱、高效液相色谱二极管阵列检测、气相色谱-质谱指纹图谱结合化学计量学对生煎苍术进行综合评价和鉴定

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-04 DOI: 10.1016/j.jpba.2025.116872

Zicheng Ma , Xiaoran Zhao , Zhiyan Xie , Mengjie Lv , Jie Gao , Lili Sun , Jia Li , Xiaoliang Ren

{"title":"Fourier transform infrared spectroscopy, high-performance liquid chromatography with diode array detection, and gas chromatography-mass spectrometry fingerprints combined with chemometrics for comprehensive evaluation and identification of raw and bran-fried Atractylodis Rhizoma","authors":"Zicheng Ma , Xiaoran Zhao , Zhiyan Xie , Mengjie Lv , Jie Gao , Lili Sun , Jia Li , Xiaoliang Ren","doi":"10.1016/j.jpba.2025.116872","DOIUrl":"10.1016/j.jpba.2025.116872","url":null,"abstract":"<div><div>The quality and therapeutic properties of Atractylodis Rhizoma (AR) can be significantly influenced by processing methods such as bran frying. This study aims to comprehensively evaluate and identify raw and bran-fried AR using FT-IR, HPLC-DAD, and GC/MS fingerprints combined with chemometric analysis. FT-IR provided characteristic spectra for qualitative analysis, while HPLC-DAD and GC/MS revealed significant differences in chemical profiles, respectively. FT-IR spectra, coupled with chemometric methods such as genetic algorithm-based backpropagation neural network (GA-BPNN), effectively distinguished between raw and bran-fried samples. Preprocessing the original spectra with second-order derivative and VISSA resulted in 100 % accuracy, precision, recall, and F1 score in the test set. HPLC fingerprint, combined with hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), successfully differentiated raw AR from its processed products. The results indicated that bran frying significantly increased the levels of atractylenolide I and atractylenolide III, while decreasing the levels of atractylon. The GC/MS fingerprint, combined with a counter propagation-artificial neural network model, effectively distinguished between raw and bran-fried products, identifying characteristic volatile markers. This study highlights the potential of combining various advanced techniques for the quality evaluation and identification of AR and its processed products, providing valuable insights for quality control and therapeutic application.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116872"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualizing the spatial distribution of functional metabolites in the root of Codonopsis pilosula: An investigation integrating metabolomics, serum pharmacochemistry, and MALDI-MSI 可视化党参根部功能代谢物的空间分布：一项结合代谢组学、血清药物化学和MALDI-MSI的研究

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-04 DOI: 10.1016/j.jpba.2025.116873

Xinjie Wu , Tongtong Li , Yan He , Hui He , Tong Tong , Boyi Fan , Jianping Gao , Jinfang Xu

{"title":"Visualizing the spatial distribution of functional metabolites in the root of Codonopsis pilosula: An investigation integrating metabolomics, serum pharmacochemistry, and MALDI-MSI","authors":"Xinjie Wu , Tongtong Li , Yan He , Hui He , Tong Tong , Boyi Fan , Jianping Gao , Jinfang Xu","doi":"10.1016/j.jpba.2025.116873","DOIUrl":"10.1016/j.jpba.2025.116873","url":null,"abstract":"<div><div><em>Codonopsis pilosula</em> is a traditional Chinese tonic herb commonly used as food and medicine, particularly for the treatment of spleen deficiency syndrome. However, the specific bioactive components responsible for <em>C. pilosula</em> effects remain unclear, and their distribution in the root of <em>C. pilosula</em> has not been fully elucidated due to the lack of efficient analytical techniques. In this work, we combined metabolomics with serum pharmacochemistry to investigate <em>C. pilosula</em> effects in a rat model of spleen deficiency syndrome. Pearson correlative analysis was applied to discover that 8 chemical compounds, including lobetyolin, atractylenolide III, syringin, luteolin, asperuloside, O-acetyl-L-serine, L-citrulline and creatine, were significantly correlated with biomarkers and considered as potential pharmacodynamic basis of <em>C. pilosula</em>. Furthermore, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was performed to visualize the spatial distribution of the bioactive substances in the root of <em>C. pilosula</em>. L-citrulline was widely distributed in roots. The content of atractylenolide III and luteolin in the cortex and xylem was much higher than that in the phloem. O-Acetyl-L-serine was present in the entire phloem and a small portion of the xylem. Creatine, lobetyolin, asperuloside, and syringin were mostly distributed in the peridermal cortex, and their contents were low. By integrating metabolomics, serum pharmacochemistry and MALDI-MSI, our proposed analytical method elucidated the material basis of <em>C. pilosula</em> and figured out their spatial distribution in the root of <em>C. pilosula</em>, which provides new insights for improving the quality of medicinal herbs.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"262 ","pages":"Article 116873"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of related substances in minocycline hydrochloride via 2D-LC-Q-TOF /MS 2D-LC-Q-TOF /MS法鉴别盐酸米诺环素中有关物质

IF 3.1 3区医学

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-04-03 DOI: 10.1016/j.jpba.2025.116862

Li Jiang , Zhaoliang Hu , Jing Jiang , Taijun Hang , Yuting Lu

引用次数: 0