European Journal of Immunology最新文献_第9页

An in vitro model for osteoarthritis using long-cultured inflammatory human macrophages repeatedly stimulated with TLR agonists 一种骨关节炎的体外模型，使用TLR激动剂反复刺激长期培养的炎症性人类巨噬细胞。

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-15 DOI: 10.1002/eji.202350507

Aldo Ummarino, Alba Pensado-López, Roberta Migliore, Lourdes Alcaide-Ruggiero, Nicholas Calà, Michele Caputo, Francesco M. Gambaro, Clément Anfray, Flavio L. Ronzoni, Elizaveta Kon, Paola Allavena, Fernando Torres Andón

{"title":"An in vitro model for osteoarthritis using long-cultured inflammatory human macrophages repeatedly stimulated with TLR agonists","authors":"Aldo Ummarino, Alba Pensado-López, Roberta Migliore, Lourdes Alcaide-Ruggiero, Nicholas Calà, Michele Caputo, Francesco M. Gambaro, Clément Anfray, Flavio L. Ronzoni, Elizaveta Kon, Paola Allavena, Fernando Torres Andón","doi":"10.1002/eji.202350507","DOIUrl":"10.1002/eji.202350507","url":null,"abstract":"Osteoarthritis (OA) is characterized by an abundance of inflammatory M1-like macrophages damaging local tissues. The search for new potential drugs for OA suffers from the lack of appropriate methods of long-lasting inflammation. Here we developed and characterized an in vitro protocol of long-lasting culture of primary human monocyte-derived macrophages differentiated with a combination of M-CSF+GM-CSF that optimally supported long-cultured macrophages (LC-Mϕs) for up to 15 days, unlike their single use. Macrophages repeatedly stimulated for 15 days with the TLR2 ligand Pam3CSK4 (LCS-Mϕs), showed sustained levels over time of IL-6, CCL2, and CXCL8, inflammatory mediators that were also detected in the synovial fluids of OA patients. Furthermore, macrophages isolated from the synovia of two OA patients showed an expression profile of inflammation-related genes similar to that of LCS-Mϕs, validating our protocol as a model of chronically activated inflammatory macrophages. Next, to confirm that these LCS-Mϕs could be modulated by anti-inflammatory compounds, we employed dexamethasone and/or celecoxib, two drugs widely used in OA treatment, that significantly inhibited the production of inflammatory mediators. This easy-to-use in vitro protocol of long-lasting inflammation with primary human macrophages could be useful for the screening of new compounds to improve the therapy of inflammatory disorders.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"53 12","pages":""},"PeriodicalIF":5.4,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202350507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10242827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation and differentiation of cognate T cells by a dextran-based antigen-presenting system for cancer immunotherapy 癌症免疫治疗用右旋糖酐抗原提呈系统激活和分化同源T细胞。

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-12 DOI: 10.1002/eji.202350528

Dhrubajyoti Mahata, Debangshu Mukherjee, Debarati Biswas, Shyam Basak, Aditya Jyoti Basak, Imlilong Jamir, Nidhi Pandey, Huma Khatoon, Dibyendu Samanta, Amit Basak, Gayatri Mukherjee

{"title":"Activation and differentiation of cognate T cells by a dextran-based antigen-presenting system for cancer immunotherapy","authors":"Dhrubajyoti Mahata, Debangshu Mukherjee, Debarati Biswas, Shyam Basak, Aditya Jyoti Basak, Imlilong Jamir, Nidhi Pandey, Huma Khatoon, Dibyendu Samanta, Amit Basak, Gayatri Mukherjee","doi":"10.1002/eji.202350528","DOIUrl":"10.1002/eji.202350528","url":null,"abstract":"Immunotherapeutic modulation of antigen-specific T-cell responses instead of the whole repertoire helps avoid immune-related adverse events. We have developed an artificial antigen-presenting system (aAPS) where multiple copies of a multimeric peptide-MHC class I complex presenting a murine class I MHC restricted ovalbumin-derived peptide (signal 1), along with a costimulatory ligand (signal 2) are chemically conjugated to a dextran backbone. Cognate naive CD8+ T cells, when treated with this aAPS underwent significant expansion and showed an activated phenotype. Furthermore, elevated expression of effector cytokines led to the differentiation of these cells to cytotoxic T lymphocytes which resulted in target cell lysis, indicative of the functional efficacy of the aAPS. CD8+ T cells with decreased proliferative potential due to repeated antigenic stimulation could also be re-expanded by the developed aAPS. Thus, the developed aAPS warrants further engineering for future application as a rapidly customizable personalized immunotherapeutic agent, incorporating patient-specific MHC-restricted tumor antigens and different costimulatory signals to modulate both naive and antigen-experienced but exhausted tumor-specific T cells in cancer.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"53 12","pages":""},"PeriodicalIF":5.4,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10564920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Joint Conference of the Société Française d'Immunologie (SFI) and the Deutsche Gesellschaft für Immunologie (DGfI), 26–29 September, 2023, Strasbourg, France 法国免疫学协会 (SFI) 和德国免疫学协会 (DGfI) 联合会议，2023 年 9 月 26-29 日，法国斯特拉斯堡

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-10 DOI: 10.1002/eji.202370300

引用次数: 0

CD8+ T cells reduce neuroretina inflammation in mouse by regulating autoreactive Th1 and Th17 cells through IFN-γ CD8+ T细胞通过IFN-γ调节自反应性Th1和Th17细胞，从而减轻小鼠神经视网膜炎症

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-09 DOI: 10.1002/eji.202350574

Sihan Wu, Xuan Zhang, Cuiping Hu, Yajie Zhong, Jun Chen, Wai Po Chong

{"title":"CD8+ T cells reduce neuroretina inflammation in mouse by regulating autoreactive Th1 and Th17 cells through IFN-γ","authors":"Sihan Wu, Xuan Zhang, Cuiping Hu, Yajie Zhong, Jun Chen, Wai Po Chong","doi":"10.1002/eji.202350574","DOIUrl":"10.1002/eji.202350574","url":null,"abstract":"Various regulatory CD8+ T-cell subsets have been proposed for immune tolerance and have been implicated in controlling autoimmune diseases. However, their phenotypic identities and suppression mechanisms are not yet understood. This study found that coculture of T-cell receptor (TCR)- or interferon (IFN)-β-activated CD8+ T cells significantly suppressed the cytokine production of Th1 and Th17 cells. By experimenting with the experimental autoimmune uveitis (EAU), we found that adoptive transfer of TCR or IFN-β-activated CD8+ T cells significantly lessened disease development in an IFN-γ-dependent manner with a decreased uveitogenic Th1 and Th17 response. Interestingly, after adoptive transfer into the EAU mice, the IFN-γ+CD8+ T cells were recruited more efficiently into the secondary lymphoid organs during the disease-priming phase. This recruitment depends on the IFN-γ-inducible chemokine receptor CXCR3; knocking out CXCR3 abolishes the protective effect of CD8+ T cells in EAU. In conclusion, we identified the critical role of IFN-γ for CD8+ T cells to inhibit Th1 and Th17 responses and ameliorate EAU. CXCR3 is necessary to recruit IFN-γ+CD8+ T cells to the secondary lymphoid organ for the regulation of autoreactive Th1 and Th17 cells.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"53 12","pages":""},"PeriodicalIF":5.4,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202350574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10284293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD40L modulates CD4+ T-cell activation through receptor for activated C kinase 1 CD40L通过活化的C激酶1受体调节CD4+ t细胞活化。

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-08 DOI: 10.1002/eji.202350520

Bram W. van Os, Winnie G. Vos, Laura A. Bosmans, Claudia M. van Tiel, Myrthe den Toom, Linda Beckers, Merel Admiraal, Marten A. Hoeksema, Menno P. de Winther, Esther Lutgens

引用次数: 0

Impressum 铭刻

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-07 DOI: 10.1002/eji.202370094

引用次数: 0

Cover Story: Eur. J. Immunol. 9'23 封面故事:欧元。[j] .免疫学杂志，2009

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-07 DOI: 10.1002/eji.202370091

引用次数: 0

Costimulatory capacity of CD137 mAbs on T cells depends on elaborate CRD structures but not on blocking ligand‒receptor binding CD137mAb对T细胞的共刺激能力取决于复杂的CRD结构，而不取决于阻断配体-受体结合。

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-07 DOI: 10.1002/eji.202350493

Xin Jin, Ling Yi, Xiaojue Wang, Zhuohong Yan, Panjian Wei, Bin Yang, Hongtao Zhang

{"title":"Costimulatory capacity of CD137 mAbs on T cells depends on elaborate CRD structures but not on blocking ligand‒receptor binding","authors":"Xin Jin, Ling Yi, Xiaojue Wang, Zhuohong Yan, Panjian Wei, Bin Yang, Hongtao Zhang","doi":"10.1002/eji.202350493","DOIUrl":"10.1002/eji.202350493","url":null,"abstract":"CD137 is mainly a costimulatory receptor of CD8+ T cells. Two representative CD137 antibodies, utomilumab, and urelumab, show different costimulatory capacities in clinical trials. Balancing the antitumor effect and systemic toxicity of T cells activated by CD137 signaling is a challenge that requires clinical consideration. In this study, a panel of specific anti-human CD137 monoclonal antibodies (mAbs) were prepared and their affinities, isotypes, CD137-CRD (cysteine-rich domain) binding regions, cross-reactivity to mouse and rhesus CD137, inhibition of ligand‒receptor binding and costimulatory activities were analyzed. The results showed that anti-human CD137 mAbs had high cross-reactivity with rhesus CD137. MAbs fell into three clusters according to their different binding regions of the CD137 extracellular domain. They bound to CRDI+CRDII, CRDIII or CRDIV+STP. CRDIII-binding mAbs had the strongest blocking activity. Highly costimulatory CD137 mAbs showed stronger abilities to promote CD8+ T-cell proliferation. However, the costimulatory capacity of mAbs on T cells was not closely related to their ability to block CD137L-CD137 binding and may be controlled by more elaborate CRD conformational structures. This study provides additional information for the development of next-generation CD137 mAbs to meet clinical needs.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"53 12","pages":""},"PeriodicalIF":5.4,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Eur. J. Immunol. 9'23 发行信息:欧元。[j] .免疫学杂志，2009

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-07 DOI: 10.1002/eji.202370092

引用次数: 0

SIRPα: A key player in innate immunity SIRPα：先天免疫的关键因素。

IF 5.4 3区医学

European Journal of Immunology Pub Date : 2023-09-06 DOI: 10.1002/eji.202350375

Yongyi Feng, Chunliu Huang, Yingzhao Wang, Jun Chen

引用次数: 0