Journal of oral tissue engineering最新文献_第6页

Influence of the ES-D3 Cell Differentiation and Cell Viability Rate by the 6 Essential Amino Acids Adding in the Minimum Essential Medium 在最低必需培养基中添加6种必需氨基酸对ES-D3细胞分化和细胞存活率的影响

Journal of oral tissue engineering Pub Date : 2015-01-01 DOI: 10.11223/JARDE.13.10

K. Imai, Tatsuro Miyake, I. Tamura, Hiroki Ishikawa, T. Iseki, S. Morita, H. Sawai, T. Ohkubo, M. Shida, T. Nishikawa, Tomoharu Okamura, A. Tanaka, K. Suese

{"title":"Influence of the ES-D3 Cell Differentiation and Cell Viability Rate by the 6 Essential Amino Acids Adding in the Minimum Essential Medium","authors":"K. Imai, Tatsuro Miyake, I. Tamura, Hiroki Ishikawa, T. Iseki, S. Morita, H. Sawai, T. Ohkubo, M. Shida, T. Nishikawa, Tomoharu Okamura, A. Tanaka, K. Suese","doi":"10.11223/JARDE.13.10","DOIUrl":"https://doi.org/10.11223/JARDE.13.10","url":null,"abstract":"SYNOPSIS Although research on regenerative medicine using iPS cells has markedly progressed, no culture medium meeting the objective of studies on cell differentiation using iPS cells has been developed. The establishment of the in vitro cell growth method and development of MEM and DMEM as cell culture media have facilitated the stable growth of many types of cultured cell. These culture media are of marked historical value in cell culture methods, and they are used for the cell differentiation of iPS and ES cells without modification as the standard media. There are only a few reports providing a basis for the essential amino acid contents of these culture media. To develop a new culture medium for cell differentiation, we started a basic investigation of the essential amino acid contents using mouse-derived ES-D3 cells which do not require feeder cells. Dulbecco's Modified Eagle Medium (DMEM) is normally used for ES-D3 cell culture. The essential amino acid content of DMEM is higher than that of Minimum Essential Medium (MEM). Of the 7 essential amino acid contained in MEM, the contents of 6 amino acids are lower than those of DMEM excluding L-arginine. Thus, we investigated the influence of the 6 essential amino acids excluding L-arginine by adding them individually to MEM, and the viability and differentiation rate of ES-D3 cells were investigated. The addition of L-glutamine improved the cell viability and differentiation rate. It was clarified that the accumulation of data on the differentiation of various cells is necessary to determine the appropriate essential amino acid contents, aiming at the preparation of culture media for regenerative medicine.","PeriodicalId":16589,"journal":{"name":"Journal of oral tissue engineering","volume":"7 1","pages":"10-17"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79780273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Study of Embryotoxicity of Commercial Dental Gold-silver-palladium Alloy by Corrosion using Acid and Artificial Saliva 用酸和人工唾液腐蚀商用牙科金银钯合金的胚胎毒性研究

Journal of oral tissue engineering Pub Date : 2015-01-01 DOI: 10.11223/JARDE.13.77

K. Imai, Y. Nishitani, Tomohiro Hoshika, M. Akiyama, S. Morita, T. Iseki, H. Yoshida, Kazuhiro Matsumoto, I. Tamura, M. Shida, H. Sawai, T. Ohkubo, K. Suese

引用次数: 4

The Effect of Artificial Recombinant Bone Morphogenetic Protein 2 with Titanium-binding Peptide on a Ti Graft in Vivo 含钛结合肽的人工重组骨形态发生蛋白2对体内钛移植物的影响

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.1

E. Kokubu, K. Kokubun, K. Matsuzaka, K. Kashiwagi, K. Shiba, Takashi Inoue, K. Ishihara, M. Yoshinari

引用次数: 1

In Vitro Study of Cell Differentiation by Two Type Mouse Embryo Stem Cells on the Dental Adhesives 两型小鼠胚胎干细胞对牙黏合剂细胞分化的体外研究

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.85

K. Imai, K. Suese, Y. Honda, Tsubasa Shirai, Fumiya Ogawa, H. Sawai, H. Takashima

引用次数: 2

Clinical Usage Experience and Evaluation of Application of Platelet Rich Plasma Gel in Impacted Tooth Extraction: A Preliminary Study 富血小板血浆凝胶在阻生牙拔除中的临床应用体会及评价

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.80

N. Fukui, T. Ueno, Y. Kimura, Y. Nakajima, Akihiro Sunano, Yuichi Ito, Shin Kasuya

引用次数: 0

Microdevice in the Oral Implant Superstructure 口腔种植体上部结构中的微型装置

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.36

K. Imai

引用次数: 2

Induction of ES cells from Human Bone Marrow Mesenchymal Stem Cells: Epigenetic Control and Initialization of the Cells 人骨髓间充质干细胞诱导胚胎干细胞:表观遗传控制和细胞初始化

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.11.220

S. Izawa, K. Yamaji, Tomohiro Hoshika, Y. Nishitani, M. Yoshiyama

引用次数: 1

Study on the Possibility of Cell Growth in Tissue Culture Medium Containing Artificial Saliva 含人工唾液组织培养基中细胞生长可能性的研究

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.8

K. Imai, H. Takashima

引用次数: 1

Effects on Circulating VEGF Concentration on Periodontal Surgery in Diabetic Rats 血管内皮生长因子循环浓度对糖尿病大鼠牙周手术的影响

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.57

Hiromasa Morita, N. Shigematsu, T. Kono, M. Umeda

{"title":"Effects on Circulating VEGF Concentration on Periodontal Surgery in Diabetic Rats","authors":"Hiromasa Morita, N. Shigematsu, T. Kono, M. Umeda","doi":"10.11223/JARDE.12.57","DOIUrl":"https://doi.org/10.11223/JARDE.12.57","url":null,"abstract":"57 INTRODUCTION The number of diabetic patients in Japan is estimated to be 7.2 million, and a total of about 22 million are considered to have, or be at a high risk of, diabetes. Diabetes is classified into types 1 (insulin-dependent) and 2 (non-insulindependent), but type 2, which has an onset in middle to old age, is predominant, accounting for about 95% of all diabetic patients. Once it develops, diabetes causes various complications, which are classified into macroand microvascular disorders. Macrovascular disorders include cerebrovascular disturbances, such as cerebral infarction, and cardiovascular disturbances, such as myocardial infarction. Microvascular disorders include retinopathy, nephropathy, and neuropathy. Vascular impairment is associated with the etiology of all these 5 diabetic complications. In 2008, the Japan Diabetes Society proposed periodontal disease as the sixth complication of diabetes mellitus. Diabetic patients frequently develop periodontal disease, and diabetes mellitus has been reported to be an important risk factor for the disease. In addition, periodontal disease in diabetic patients tends to be severe and often requires periodontal surgery for its treatment. Effects on Circulating VEGF Concentration on Periodontal Surgery in Diabetic Rats","PeriodicalId":16589,"journal":{"name":"Journal of oral tissue engineering","volume":"92 1","pages":"57-68"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80339629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Novel Research Targets of Regenerative Medicine 再生医学的新研究目标

Journal of oral tissue engineering Pub Date : 2014-01-01 DOI: 10.11223/JARDE.12.100

K. Imai

引用次数: 2