Journal of neurobiology最新文献_第3页

High-resolution imaging demonstrates dynein-based vesicular transport of activated Trk receptors. 高分辨率成像显示激活的Trk受体基于动力蛋白的囊泡运输。

Journal of neurobiology Pub Date : 2002-06-15 DOI: 10.1002/NEU.10062

A. Bhattacharyya, F. L. Watson, S. Pomeroy, Yan-zhen Zhang, C. Stiles, R. Segal

引用次数: 102

L1 mediated homophilic binding and neurite outgrowth are modulated by alternative splicing of exon 2. L1介导的亲同性结合和神经突生长是由外显子2的选择性剪接调节的。

Journal of neurobiology Pub Date : 2002-06-05 DOI: 10.1002/NEU.10052

J. Jacob, J. Haspel, Noriko Kane-Goldsmith, M. Grumet

{"title":"L1 mediated homophilic binding and neurite outgrowth are modulated by alternative splicing of exon 2.","authors":"J. Jacob, J. Haspel, Noriko Kane-Goldsmith, M. Grumet","doi":"10.1002/NEU.10052","DOIUrl":"https://doi.org/10.1002/NEU.10052","url":null,"abstract":"The neural cell adhesion molecule (CAM) L1 is a member of the immunoglobulin superfamily that has been implicated in neuronal adhesion, neurite outgrowth, and axon guidance. The clinical importance of L1 is illustrated by pathological mutations that lead to hydrocephalus, mental retardation, motor defects, and early mortality. The L1 gene is composed of 28 exons, including exons 2 and 27 that are spliced alternatively, and mutations in exon 2 are associated with severe neurological abnormalities in humans. To elucidate the role of L1 exon 2, a recombinant Fc fusion protein called Delta2L1 was constructed lacking the second exon in the extracellular domain of L1. When bound to fluorescent beads, L1 exhibited homophilic binding while Delta2L1 did not. However, L1 beads coaggregated with the Delta2L1 beads. Similarly, in cell binding studies, L1 bound to L1 and Delta2L1 did not bind to Delta2L1 but it bound moderately to L1. Given the reduced binding of Delta2L1, we tested its effect on neurons. By comparison to L1, a lower percentage of dissociated neurons extended neurites on Delta2L1, and there was a modest decrease in the length of the neurites that grew. Neurite outgrowth from reaggregated neurons was much less robust on Delta2L1 than on L1. The combined results indicate that Delta2L1 does not bind homophilically but it can interact with L1 containing exon 2. The reduced binding and neurite promoting activity of Delta2L1 provides an explanation for certain pathological mutations in L1 that lead to clinically apparent disease in the absence of the normal form of L1 in the nervous system.","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"17 1","pages":"177-89"},"PeriodicalIF":0.0,"publicationDate":"2002-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78812699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP. IgLON家族成员neurotrimin和LAMP之间的互补表达和异亲性相互作用。

Journal of neurobiology Pub Date : 2002-06-05 DOI: 10.1002/NEU.10050

O. Gil, Li Zhang, Suzanne Chen, Y. Ren, A. Pimenta, G. Zanazzi, D. Hillman, P. Levitt, J. Salzer

{"title":"Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP.","authors":"O. Gil, Li Zhang, Suzanne Chen, Y. Ren, A. Pimenta, G. Zanazzi, D. Hillman, P. Levitt, J. Salzer","doi":"10.1002/NEU.10050","DOIUrl":"https://doi.org/10.1002/NEU.10050","url":null,"abstract":"Neurotrimin (Ntm) and the limbic system-associated membrane protein (LAMP) are members of the IgLON (LAMP, OBCAM, Ntm) family of glycorylphosphatidylinositol anchored neural cell adhesion molecules. We previously reported that LAMP and Ntm promote adhesion and neurite outgrowth via a homophilic mechanism, suggesting that these proteins promote the formation of specific neuronal circuits by homophilic interactions. In this report, we have further characterized the expression and binding specificity of Ntm. Using a newly generated monoclonal antibody to Ntm, we demonstrated that this protein is largely expressed in a complementary pattern to that of LAMP in the nervous system, with co-expression at a few sites. Ntm is expressed at high levels in sensory-motor cortex and, of particular note, is transiently expressed in neurons of cortical barrel fields and corresponding thalamic \"barreloids.\" Binding of a recombinant, soluble form of Ntm to CHO cells expressing either Ntm or LAMP demonstrates that Ntm and LAMP interact both homophilically and heterophilically. In contrast to conventional growth-promoting activity of Ig superfamily members, LAMP strongly inhibits the outgrowth of Ntm-expressing dorsal root ganglion (DRG) neurons in a heterophilic manner. These anatomical and functional data support the concept that homophilic and heterophilic interactions between IgLON family members are likely to play a role in the specification of neuronal projections via growth promoting and inhibiting effects, respectively.","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"12 1","pages":"190-204"},"PeriodicalIF":0.0,"publicationDate":"2002-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81952016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

Multiphoton stimulation of neurons. 神经元的多光子刺激。

Journal of neurobiology Pub Date : 2002-06-05 DOI: 10.1002/NEU.10056

H. Hirase, Volodymyr Nikolenko, J. Goldberg, R. Yuste

引用次数: 163

Septum volume and food-storing behavior are related in parids. 雌雄同体中隔膜体积与食物储存行为有关。

Journal of neurobiology Pub Date : 2002-06-05 DOI: 10.1002/NEU.10054

Michael W Shiflett, K. Gould, T. Smulders, T. Devoogd

引用次数: 31

Forked end: a novel transmembrane protein involved in neuromuscular specificity in drosophila identified by gain-of-function screening. 分叉端:一种新的跨膜蛋白参与神经肌肉特异性在果蝇的功能获得筛选鉴定。

Journal of neurobiology Pub Date : 2002-06-05 DOI: 10.1002/NEU.10063

T. Umemiya, Etsuko Takasu, M. Takeichi, T. Aigaki, A. Nose

引用次数: 12

Inactivation of olfactory sensilla of a single morphological type differentially affects the response of Drosophila to odors. 单一形态类型的嗅感觉器失活对果蝇对气味的反应有不同的影响。

Journal of neurobiology Pub Date : 2002-06-05 DOI: 10.1002/NEU.10057

S.-K. Park, S. Shanbhag, A. Dubin, M. de Bruyne, Q. Wang, P. Yu, N. Shimoni, S. D'Mello, J. Carlson, G. L. Harris, R. Steinbrecht, C. Pikielny

{"title":"Inactivation of olfactory sensilla of a single morphological type differentially affects the response of Drosophila to odors.","authors":"S.-K. Park, S. Shanbhag, A. Dubin, M. de Bruyne, Q. Wang, P. Yu, N. Shimoni, S. D'Mello, J. Carlson, G. L. Harris, R. Steinbrecht, C. Pikielny","doi":"10.1002/NEU.10057","DOIUrl":"https://doi.org/10.1002/NEU.10057","url":null,"abstract":"The olfactory organs on the head of Drosophila, antennae and maxillary palps, contain several hundred olfactory hairs, each with one or more olfactory receptor neurons. Olfactory hairs belong to one of three main morphological types, trichoid, basiconic, and coeloconic sensilla, and show characteristic spatial distribution patterns on the surface of the antenna and maxillary palps. Here we show that targeting expression of the cell-death gene reaper to basiconic sensilla (BS) causes the specific inactivation of most olfactory sensilla of this type with no detectable effect on other types of olfactory sensilla or the structure of the antennal lobe. Our data suggest that BS are required for a normal sensitivity to many odorants with a variety of chemical structures, through a wide range of concentrations. Interestingly, however, in contrast to other odorants tested, the behavioral response of ablated flies to intermediate concentrations of propionic and butyric acids is normal, suggesting the involvement of sensilla unaffected by ectopic reaper expression, probably coeloconic sensilla that respond strongly to these two organic acids. As inactivation of BS causes an underestimation of the concentration of both acids detectable at both the highest and lowest odorants concentrations, our results suggest that concentration coding for these two odorants relies on the integration of signals from different subsets of sensilla, most likely of different morphological types.","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"18 1","pages":"248-60"},"PeriodicalIF":0.0,"publicationDate":"2002-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88992148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Changes in spatial memory mediated by experimental variation in food supply do not affect hippocampal anatomy in mountain chickadees (Poecile gambeli). 实验性食物供应变化介导的空间记忆变化不影响山山雀海马解剖结构。

Journal of neurobiology Pub Date : 2002-05-01 DOI: 10.1002/NEU.10045

V. Pravosudov, Pierre Lavenex, N. S. Clayton

{"title":"Changes in spatial memory mediated by experimental variation in food supply do not affect hippocampal anatomy in mountain chickadees (Poecile gambeli).","authors":"V. Pravosudov, Pierre Lavenex, N. S. Clayton","doi":"10.1002/NEU.10045","DOIUrl":"https://doi.org/10.1002/NEU.10045","url":null,"abstract":"Earlier reports suggested that seasonal variation in food-caching behavior (caching intensity and cache retrieval accuracy) might correlate with morphological changes in the hippocampal formation, a brain structure thought to play a role in remembering cache locations. We demonstrated that changes in cache retrieval accuracy can also be triggered by experimental variation in food supply: captive mountain chickadees (Poecile gambeli) maintained on limited and unpredictable food supply were more accurate at recovering their caches and performed better on spatial memory tests than birds maintained on ad libitum food. In this study, we investigated whether these two treatment groups also differed in the volume and neuron number of the hippocampal formation. If variation in memory for food caches correlates with hippocampal size, then our birds with enhanced cache recovery and spatial memory performance should have larger hippocampal volumes and total neuron numbers. Contrary to this prediction we found no significant differences in volume or total neuron number of the hippocampal formation between the two treatment groups. Our results therefore indicate that changes in food-caching behavior and spatial memory performance, as mediated by experimental variations in food supply, are not necessarily accompanied by morphological changes in volume or neuron number of the hippocampal formation in fully developed, experienced food-caching birds.","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"57 1","pages":"142-8"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84719930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Comparison of the effects of HGF, BDNF, CT-1, CNTF, and the branchial arches on the growth of embryonic cranial motor neurons. HGF、BDNF、CT-1、CNTF和鳃弓对胚胎颅运动神经元生长影响的比较

Journal of neurobiology Pub Date : 2002-05-01 DOI: 10.1002/NEU.10048

Arifa Naeem, L. Abbas, S. Guthrie

{"title":"Comparison of the effects of HGF, BDNF, CT-1, CNTF, and the branchial arches on the growth of embryonic cranial motor neurons.","authors":"Arifa Naeem, L. Abbas, S. Guthrie","doi":"10.1002/NEU.10048","DOIUrl":"https://doi.org/10.1002/NEU.10048","url":null,"abstract":"In the developing embryo, axon growth and guidance depend on cues that include diffusible molecules. We have shown previously that the branchial arches and hepatocyte growth factor (HGF) are growth-promoting and chemoattractant for young embryonic cranial motor axons. HGF is produced in the branchial arches of the embryo, but a number of lines of evidence suggest that HGF is unlikely to be the only factor involved in the growth and guidance of these axons. Here we investigate whether other neurotrophic factors could be involved in the growth of young cranial motor neurons in explant cultures. We find that brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT-1) all promote the outgrowth of embryonic cranial motor neurons, while glial cell line-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) fail to affect outgrowth. We next examined whether HGF and the branchial arches had similar effects on motor neuron subpopulations at different axial levels. Our results show that HGF acts as a generalized rather than a specific neurotrophic factor and guidance cue for cranial motor neurons. Although the branchial arches also had general growth-promoting effects on all motor neuron subpopulations, they chemoattracted different axial levels differentially, with motor neurons from the caudal hindbrain showing the most striking response.","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"36 1","pages":"101-14"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85441144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Developmental and hormonal regulation of NR2A mRNA in forebrain regions controlling avian vocal learning. NR2A mRNA在控制鸟类声音学习的前脑区域的发育和激素调节。

Journal of neurobiology Pub Date : 2002-05-01 DOI: 10.1002/NEU.10046

J. Heinrich, T. D. Singh, F. Sohrabji, K. Nordeen, E. Nordeen

{"title":"Developmental and hormonal regulation of NR2A mRNA in forebrain regions controlling avian vocal learning.","authors":"J. Heinrich, T. D. Singh, F. Sohrabji, K. Nordeen, E. Nordeen","doi":"10.1002/NEU.10046","DOIUrl":"https://doi.org/10.1002/NEU.10046","url":null,"abstract":"Developmental changes in the composition of NMDA receptors can alter receptor physiology as well as intracellular signal transduction cascades, potentially shifting thresholds for neural and behavioral plasticity. During song learning in zebra finches, NMDAR currents become faster, and transcripts for the modulatory NR2B subunit of this receptor decrease in lMAN, a region in which NMDAR activation is critical for vocal learning. Using in situ hybridization, we found that NR2A transcripts change reciprocally, increasing significantly in both lMAN (59%) and in another song region, Area X (38%), between posthatch day (PHD) 20 and 40, but not changing further at PHD60 or 80. In adjacent areas not associated with song learning, NR2A mRNA did not change between PHD20-80. Although early song deprivation (which extends the sensitive period for song learning) delays changes in NR2B gene expression and NMDAR physiology within the lMAN, it did not alter NR2A mRNA levels measured at PHD40, 45, or 60. Early testosterone (T) treatment, which disrupts vocal development and accelerates the maturation of both NR2B levels and NMDAR physiology in lMAN, also significantly increased NR2A transcripts measured at PHD35 in lMAN. In Area X, a similar effect of T approached significance. Together with our previous studies, these results show that in a pathway critical for vocal plasticity, the ratio of NR2A:NR2B mRNA rises abruptly early during the sensitive period for song learning. Furthermore, androgen regulation of NMDAR gene expression may alter thresholds for experience-dependent synaptic change.","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"110 1","pages":"149-59"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80516185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29