Journal of medical microbiology最新文献

{"title":"A comparative analysis of the second and third wave of the Covid-19 pandemic: an experience from a tertiary care hospital in Western India.","authors":"Anjali Swami,&nbsp;Ankita Mohanty,&nbsp;Ashima Jamwal,&nbsp;Dilip Turbadkar,&nbsp;Sujata Baveja,&nbsp;Jayanthi Shastri,&nbsp;Vidushi Chitalia","doi":"10.1099/jmm.0.001685","DOIUrl":"https://doi.org/10.1099/jmm.0.001685","url":null,"abstract":"<p><p><b>Introduction.</b> As the world was still recovering from the 2020 pandemic, the devastating impact of Covid-19 driven by the Delta variant shook the world in 2021. As the second wave was declining, there was an unusual surge in Covid-19 positive cases by the end of 2021 which led to global concern about the change in virus characteristics.<b>Hypothesis/gap statement.</b> Whole genome sequencing is critical for understanding a rapidly progressing pandemic.<b>Aim</b>. To provide an insight into the major differences encountered in the changing characteristics between the second and third waves of the pandemic at a tertiary care hospital in India.<b>Methods.</b> A retrospective observational cohort analysis was conducted on Covid-positive patients during the second wave of the Covid-19 pandemic (from March 2021 to April 2021) and the third wave of the Covid-19 pandemic (from December 2021 to January 2022).<b>Results.</b> Out of 303 Covid-19 positive cases, 52 samples were tested by whole genome sequencing during the second wave and 108 during the third wave. A decline of 18.5 % was observed in the case fatality rate from the second wave to the third wave. There was a 5 % decline in the number of patients admitted with ARDS and a 16.3 % decline in the number of patients with co-morbidities.In total, 51.9 percent of cases were due to the Delta variant during the second wave and 95 percent due to the Omicron variant during the third wave. We found that 36.5 % of Covid-positive patients during the second wave had been vaccinated compared to 40 % in the third wave.<b>Conclusion.</b> Whole genome sequencing of clinical samples from a wide range of individuals during a viral epidemic will enable us to develop a more rapid public health response to new variants and identify the required vaccine modifications more quickly.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9459759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meeting Report for Bridging the Clinical-Research Gap 2022: a collaborative event between the Healthcare Infection Society and the Microbiology Society.","authors":"Curtis O Asante,&nbsp;Aggie Bak,&nbsp;Christine Fears","doi":"10.1099/jmm.0.001697","DOIUrl":"https://doi.org/10.1099/jmm.0.001697","url":null,"abstract":"","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an immunochromatographic lateral flow assay to rapidly detect OXA-23-, OXA-40-, OXA-58- and NDM-mediated carbapenem resistance determinants in <i>Acinetobacter baumannii</i>.","authors":"Sonja Mertins,&nbsp;Paul G Higgins,&nbsp;Caroline Thunissen,&nbsp;Henri Magein,&nbsp;Quentin Gilleman,&nbsp;Pascal Mertens,&nbsp;María González Rodríguez,&nbsp;Liza Marie Maus,&nbsp;Harald Seifert,&nbsp;Martin Krönke,&nbsp;Alexander Klimka","doi":"10.1099/jmm.0.001681","DOIUrl":"https://doi.org/10.1099/jmm.0.001681","url":null,"abstract":"<p><p><b>Introduction.</b> <i>Acinetobacter baumannii</i> infections can be extremely challenging to treat owing to the worldwide prevalence of multidrug-resistant isolates, especially against carbapenems. Colonization with carbapenem-resistant <i>A. baumannii</i> (CRAb) requires rapid action from an infection control perspective because the organism is known for its propensity for epidemic spread<b>.</b> <b>Hypothesis/Gap Statement.</b> There is an unmet medical need to rapidly identify CRAb to enable appropriate antimicrobial treatment and to prevent transmission<b>.</b> <b>Aim.</b> Our aim was to expand the OXA-detection abilities of the rapid immunochromatographic test (ICT) OXA-23 <i>K</i>-SeT (Coris BioConcept) to include OXA-40- and OXA-58-like carbapenemases, which together confer carbapenem resistance to more than 94 % of CRAb isolates worldwide<b>.</b> <b>Methodology.</b> We used hybridoma technology to generate mAbs against OXA-40 and OXA-58 and selected them for productivity and specificity against recombinant and endogenous OXA-40 and OXA-58. Combinations of the resulting mAbs were analysed in ICT format for their ability to detect recombinant rOXA-40_His6 or rOXA-58_His6, respectively. Subsequently, selected antibody pairs were implemented into single-OXA-40 or single-OXA-58 prototypes and the final OXA-23/40/58/NDM ICT and were evaluated on clinical <i>Acinetobacter</i> spp. isolates with well-defined carbapenem resistance mechanisms<b>.</b> <b>Results.</b> Five anti-OXA-40 and anti-OXA-58 mAbs were selected. Competition ELISA with combinations of these antibodies revealed that the anti-OXA-40 antibodies bind to one of two binding clusters on OXA-40, while anti-OXA-58 antibodies bind to one of four binding clusters on OXA-58. Direct binding to the corresponding antigen in an ICT format has left only three antibodies against rOXA-40_His6 and rOXA-58_His6, respectively for the subsequent sandwich ICT selection procedure, which revealed that the anti-OXA-40 (#5) and anti-OXA-58 (#A8) mAbs in combination with the cross-reactive mAb #C8 performed best. They were implemented into single-OXA-40 and single-OXA-58 ICT prototypes and evaluated. These single ICT prototypes demonstrated 100 % specificity and sensitivity. Based on these results, an OXA-23/40/58/NDM-ICT was developed, complemented with OXA-23 and NDM-specific detection. An evaluation with selected carbapenem-resistant <i>Acinetobacter</i> spp. isolates (<i>n</i>=34) showed 100 % specificity<b>.</b> <b>Conclusion.</b> With this easy-to-use detection assay, one can save 12-48 h in diagnostics, which helps to treat patients earlier with appropriate antibiotics and allows immediate intervention to control transmission of CRAb<b>.</b></p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9346358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CpxRA two-component system represses gene expression of the heat-labile toxin of enterotoxigenic <i>Escherichia coli</i>.","authors":"Diana Rodríguez-Valverde,&nbsp;Nancy León-Montes,&nbsp;Tania Siqueiros-Cendón,&nbsp;Sandra Rivera-Gutiérrez,&nbsp;Miguel A Ares,&nbsp;Miguel A De la Cruz","doi":"10.1099/jmm.0.001682","DOIUrl":"https://doi.org/10.1099/jmm.0.001682","url":null,"abstract":"<p><p>Enterotoxigenic <i>Escherichia coli</i> (ETEC) strains produce at least one of two types of enterotoxins: the heat-labile (LT) and heat-stable (ST) toxins, which are responsible for the watery secretory diarrhoea that is a hallmark of the human ETEC infection. One regulatory system that controls the transcription of virulence genes in pathogenic bacteria is the CpxRA two-component system (TCS). We reported that the <i>eltAB</i> bicistronic operon, which encodes for the A and B subunits of LT, was repressed for the CpxRA TCS by direct binding of CpxR-P from -12 to +6 bp with respect to the transcription start site of <i>eltAB</i>. Moreover, the Cpx-response activation down-regulated the transcription of <i>eltAB</i> genes, and this negative effect was CpxRA-dependent. Our data show that CpxRA TCS is a negative regulator of the LT, one of the main virulence determinants of ETEC.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9647438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Panton-Valentine leucocidin (PVL) toxin associated with poor clinical outcomes in patients with community-acquired <i>Staphylococcus aureus</i> bacteraemia?","authors":"Emma McGuire,&nbsp;Claire Neill,&nbsp;Simon M Collin,&nbsp;Hannah Higgins,&nbsp;Rebecca Guy,&nbsp;Mark Ganner,&nbsp;Juliana Coelho,&nbsp;Bruno Pichon,&nbsp;Russell Hope,&nbsp;Colin S Brown","doi":"10.1099/jmm.0.001683","DOIUrl":"https://doi.org/10.1099/jmm.0.001683","url":null,"abstract":"<p><p><b>Introduction.</b> Panton-Valentine leucocidin (PVL) toxin is a potential determinant of virulence associated with <i>S. aureus</i> infection.<b>Gap Statement.</b> The contribution of PVL to <i>S. aureus</i> pathogenicity remains unclear.<b>Aim.</b> To compare clinical outcomes in hospitalized patients with PVL-positive and PVL-negative community-acquired (CA) <i>S. aureus</i> bacteraemia.<b>Methods.</b> Three national datasets were combined to provide clinical and mortality data for patients with CA <i>S. aureus</i> blood culture isolates sent to the UK reference laboratory for PVL testing, August 2018 to August 2021. Multivariable logistic regression models were built for the effect of PVL positivity on 30 day all-cause mortality and 90 day readmission.<b>Results.</b> In 2191 cases of CA <i>S. aureus</i> bacteraemia, there was no association between PVL and mortality (adjusted odds ratio, aOR: 0·90, 95 % confidence interval, CI: 0·50-1·35, <i>P</i>=0·602) and no difference in median LOS (14 versus 15 days, <i>P</i>=0.169). PVL-positive cases had lower odds of readmission (aOR 0·74, CI 0·55-0.98, <i>P</i>=0·038). There was no evidence that MRSA status modified this effect (<i>P</i>=0·207).<b>Conclusions.</b> In patients with CA <i>S. aureus</i> bacteraemia PVL toxin detection was not associated with worse outcomes.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9721653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2022 Fleming Prize Lecture: diet-microbe-host interaction in early life.","authors":"Christopher J Stewart","doi":"10.1099/jmm.0.001662","DOIUrl":"https://doi.org/10.1099/jmm.0.001662","url":null,"abstract":"<p><p>The last decade has witnessed a meteoric rise in research focused on characterizing the human microbiome and identifying associations with disease risk. The advent of sequencing technology has all but eradicated gel-based fingerprinting approaches for studying microbial ecology, while at the same time traditional microbiological culture is undergoing a renaissance. Although multiplexed high-throughput sequencing is relatively new, the discoveries leading to this are nearly 50 years old, coinciding with the inaugural Microbiology Society Fleming Prize lecture. It was an honour to give the 2022 Fleming Prize lecture and this review will cover the topics from that lecture. The focus will be on the bacterial community in early life, beginning with term infants before moving on to infants delivered prematurely. The review will discuss recent work showing how human milk oligosaccharides (HMOs), an abundant but non-nutritious component of breast milk, can modulate infant microbiome and promote the growth of <i>Bifidobacterium</i> spp. This has important connotations for preterm infants at risk of necrotizing enterocolitis, a devastating intestinal disease representing the leading cause of death and long-term morbidity in this population. With appropriate mechanistic studies, it may be possible to harness the power of breast milk bioactive factors and infant gut microbiome to improve short- and long-term health in infants.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9721656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The change of inflammatory status and vaginal flora in pregnant women with premature rupture of membranes.","authors":"Shukun Gai,&nbsp;Qian Wu,&nbsp;Huijie Zhang","doi":"10.1099/jmm.0.001678","DOIUrl":"https://doi.org/10.1099/jmm.0.001678","url":null,"abstract":"<p><p><b>Introduction.</b> Premature rupture of the membrane (PROM) can trigger significant maternal complications, even maternal and fetal morbidity or mortality.<b>Hypothesis.</b> Inflammatory status and vaginal flora might be utilized to predict the occurrence of PROM.<b>Aim</b>. To explore the association between the occurrence of PROM and vaginal flora and inflammatory status alteration.<b>Methodology.</b> A case-control cross-sectional study was carried out on 140 pregnant women with or without PROM. Socio-demographic characteristics, vaginal flora assessment, pregnant outcomes and Apgar score information were retrieved.<b>Results.</b> Pregnant women with PROM showed an increased incidence of vulvovaginal candidiasis (VVC), trichomonas vaginitis (TV) and bacterial vaginitis (BV) with dysregulated vaginal flora and diminished fetal tolerance of labour indicated by down-regulated Apgar score. The increased rate of prematurity, puerperal infection and neonatal infection could be detected in PROM patients with imbalanced vaginal flora compared with PROM patients with normal vaginal flora. ROC analysis suggested IL-6 and TNF-α yielded the best discrimination for the prediction of PROM.<b>Conclusion.</b> Altered vaginal and inflammatory status are associated with PROM, and IL-6 and TNF-α can predict the occurrence of PROM.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9721652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of multiplex real-time PCR for detection of clarithromycin resistance genes for the <i>Mycobacterium abscessus</i> group.","authors":"Carolina Salgado Pedace,&nbsp;Maria Gisele Gonçalves,&nbsp;Andréia Rodrigues Souza,&nbsp;Fernanda Cristina Dos Santos Simeão,&nbsp;Natalia Fernandes Garcia de Carvalho,&nbsp;Juliana Failde Gallo,&nbsp;Erica Chimara","doi":"10.1099/jmm.0.001670","DOIUrl":"https://doi.org/10.1099/jmm.0.001670","url":null,"abstract":"<p><p><b>Introduction.</b> The <i>M. abscessus</i> molecular identification and its drug-resistance profile are important to choose the correct therapy.<b>Aim.</b> This work developed a multiplex real-time PCR (mqPCR) for detection of clarithromycin resistance genes for the <i>Mycobacterium abscessus</i> group.<b>Methodology.</b> Isolates received by Adolfo Lutz Institute from 2010 to 2012, identified by PCR restriction enzyme analysis of a fragment of the <i>hsp</i>65 gene (PRA-<i>hsp</i>65) as <i>M. abscessus</i> type 1 (<i>n</i>=135) and 2 (<i>n</i>=71) were used. Drug susceptibility test (DST) for CLA were performed with reading on days 3 and 14. Subespecies identification by <i>hsp</i>65 and <i>rpo</i>B genes sequencing and <i>erm</i>(41) and <i>rrl</i> genes for mutation detection and primer design were performed. <i>erm</i>(41) gene deletion was detected by conventional PCR. Primers and probes were designed for five detections: <i>erm</i>(41) gene full size and with deletion; <i>erm</i>(41) gene T28 and C28; <i>rrl</i> gene A2058.<b>Results.</b> In total, 191/206 (92.7 %) isolates were concordant by all methods and 13/206 (6.3 %) were concordant only between molecular methods. Two isolates (1.0 %) were discordant by mqPCR compared to <i>rrl</i> gene sequencing. The mqPCR obtained 204/206 (99.0 %) isolates in agreement with the gold standard, with sensitivity and specificity of 98 and 100 %, respectively, considering the gold standard method and 92 and 93 % regarding DST.<b>Conclusion.</b> The mqPCR developed by us proved to be an easy-to-apply tool, minimizing time, errors and contamination.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9507254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MARGINAL NOTES, February 2023. Picking through the rubble.","authors":"Timothy J J Inglis","doi":"10.1099/jmm.0.001679","DOIUrl":"https://doi.org/10.1099/jmm.0.001679","url":null,"abstract":"","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9412647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> susceptibility profiles of <i>Candida parapsilosis</i> species complex subtypes from deep infections to nine antifungal drugs.","authors":"Wei Zhang,&nbsp;Minghua Zhan,&nbsp;Na Wang,&nbsp;Jingjing Fan,&nbsp;Xuying Han,&nbsp;Caiqing Li,&nbsp;Jinlu Liu,&nbsp;Jia Li,&nbsp;Yongwang Hou,&nbsp;Xinsheng Wang,&nbsp;Zhihua Zhang","doi":"10.1099/jmm.0.001640","DOIUrl":"https://doi.org/10.1099/jmm.0.001640","url":null,"abstract":"<p><p><b>Introduction.</b> The <i>Candida parapsilosis</i> complex can be divided into <i>C. parapsilosis sensu stricto</i>, <i>C. orthopsilosis</i>, and <i>C. metapsilosis</i> subtypes. It is uncommon for drug sensitivity tests to type them.<b>Gap Statement.</b> In routine susceptibility reports, drug susceptibility of <i>C. parapsilosis</i> complex subtypes is lacking.<b>Aim.</b> The aim of this study is to investigate the antifungal susceptibility and clinical distribution characteristics of the <i>C. parapsilosis</i> complex subtypes causing deep infection in patients.<b>Methodology.</b> Non-repetitive strains of <i>C. parapsilosis</i> complex isolated from deep infection from 2017 to 2019 were collected. Species-level identification was performed using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer and confirmed using <i>ITS</i> gene sequencing, when necessary. Antifungal susceptibility testing was performed using the Sensititre YeastOne system method.<b>Results.</b> A total of 244 cases were included in the study, including 176 males (72.13 %, 60.69±13.43 years) and 68 females (27.87 %, 60.21±10.59 years). The primary diseases were cancer (43.44 %), cardiovascular disease (25.00 %), digestive system diseases, (18.44 %), infection (6.97 %), and nephropathy (6.15 %). Strains were isolated from the bloodstream (63.11 %), central venous catheters (15.16 %), pus (6.56 %), ascites (5.74 %), sterile body fluid (5.33 %), and bronchoalveolar lavage fluid (BALF, 4.09 %). Of the 244 <i>C. parapsilosis</i> complex strains, 179 (73.26 %) were identified as <i>C. parapsilosis sensu stricto</i>, 62 (25.41 %) were <i>C. orthopsilosis</i>, and three (1.23 %) were <i>C. metapsilosis</i>. Only one <i>C. parapsilosis sensu stricto</i> strain was resistant to anidulafungin, micafungin, caspofungin, and voriconazole, and it was non-wild-type (NWT) to amphotericin B. Furthermore, six <i>C. parapsilosis sensu stricto</i> strains were resistant to fluconazole, and one was dose-dependent susceptible. Five <i>C. parapsilosis sensu stricto</i> strains were NWT to posaconazole. Only one <i>C. orthopsilosis</i> strain was NWT for anidulafungin, micafungin, caspofungin, fluconazole, voriconazole, amphotericin B, and posaconazole, while the rest of the strains were wild-type.<b>Conclusion.</b> <i>C. parapsilosis sensu stricto</i> was the main clinical isolate from the <i>C. parapsilosis</i> complex in our hospital. Most strains were isolated from the bloodstream. The susceptibility rate to commonly used antifungal drugs was more than 96 %. Furthermore, most of the infected patients were elderly male cancer patients.</p>","PeriodicalId":16343,"journal":{"name":"Journal of medical microbiology","volume":"72 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9507264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}