Diana Rodríguez-Valverde, Nancy León-Montes, Tania Siqueiros-Cendón, Sandra Rivera-Gutiérrez, Miguel A Ares, Miguel A De la Cruz
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The CpxRA two-component system represses gene expression of the heat-labile toxin of enterotoxigenic Escherichia coli.
Enterotoxigenic Escherichia coli (ETEC) strains produce at least one of two types of enterotoxins: the heat-labile (LT) and heat-stable (ST) toxins, which are responsible for the watery secretory diarrhoea that is a hallmark of the human ETEC infection. One regulatory system that controls the transcription of virulence genes in pathogenic bacteria is the CpxRA two-component system (TCS). We reported that the eltAB bicistronic operon, which encodes for the A and B subunits of LT, was repressed for the CpxRA TCS by direct binding of CpxR-P from -12 to +6 bp with respect to the transcription start site of eltAB. Moreover, the Cpx-response activation down-regulated the transcription of eltAB genes, and this negative effect was CpxRA-dependent. Our data show that CpxRA TCS is a negative regulator of the LT, one of the main virulence determinants of ETEC.
期刊介绍：
Journal of Medical Microbiology provides comprehensive coverage of medical, dental and veterinary microbiology, and infectious diseases. We welcome everything from laboratory research to clinical trials, including bacteriology, virology, mycology and parasitology. We publish articles under the following subject categories: Antimicrobial resistance; Clinical microbiology; Disease, diagnosis and diagnostics; Medical mycology; Molecular and microbial epidemiology; Microbiome and microbial ecology in health; One Health; Pathogenesis, virulence and host response; Prevention, therapy and therapeutics
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