Journal of Life Science最新文献_第8页

Inhibitory Effect of Ethanol Extract of Monascus-fermented Red Yeast Rice on Proinflammatory iNOS and COX-2 Protein Expression in LPS-stimulated RAW 264.7 Macrophage Cells 红曲米乙醇提取物对lps刺激的RAW 264.7巨噬细胞促炎iNOS和COX-2蛋白表达的抑制作用

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.4.352

Ki-Hyun Kim, Jung-Hyeong Lee, G. Kwon, E. Seo, Jung-Bok Lee

引用次数: 1

Regulation of Abiotic Stress Response by Alternative Splicing in Plants 植物选择性剪接对非生物胁迫响应的调控

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.6.570

Hye-Yeon Seok, S. Lee, Y. Moon

引用次数: 0

중선포천, 관곡천, 광양서천에서 염생식물과 수생식물상 분포 中仙浦川，冠谷川，光阳舒川的盐生植物和水生植物分布

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.2.186

M. Huh

引用次数: 0

Inhibitory Effects of Tenebrio molitor Larvae Ethanol Extract on RANKL-Induced Osteoclast Differentiation 黄粉虫幼虫乙醇提取物对rankl诱导的破骨细胞分化的抑制作用

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.11.983

M. Seo, Minhee Baek, H. Lee, Yong pyo Shin, Joon-Ha Lee, In-woo Kim, Mi-Ae Kim, Jae‐Sam Hwang

{"title":"Inhibitory Effects of Tenebrio molitor Larvae Ethanol Extract on RANKL-Induced Osteoclast Differentiation","authors":"M. Seo, Minhee Baek, H. Lee, Yong pyo Shin, Joon-Ha Lee, In-woo Kim, Mi-Ae Kim, Jae‐Sam Hwang","doi":"10.5352/JLS.2020.30.11.983","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.11.983","url":null,"abstract":"The balance between bone-resorbing osteoclasts and bone-forming osteoblasts is key to bone health. An imbalance between osteoclasts and osteoblasts leads to various bone-related disorders, such as osteoporosis, osteomalacia, and osteopetrosis. However, the bone-resorption inhibitor drugs that are currently used may cause side effects. Natural substances have recently received much attention as therapeutic drugs for the treatment of bone health. This study was designed to determine the effect of Tenebrio molitor larvae ethanol extract (TME) on receptor activator of nuclear factor-κB ligand (RANKL)induced osteoclast differentiation. To measure the effect of TME on osteoclast differentiation, RAW264.7 cells were treated with RANKL with or without TME for 5 days. The tartrate-resistant acid phosphatase (TRAP) activity was significantly inhibited by treatment of TME without cytotoxicity up to 2 mg/ ml. In addition, TME effectively suppressed expression of osteoclast differentiation-related marker genes and proteins such as TRAP, NFATc1, and c-Src. TME also significantly inhibited the p38 mitogen-activated protein kinase (MAPK) signaling pathway without affecting ERK and JNK signaling in RANKL-induced RAW264.7 cells. Consequently, we conclude that TME suppresses osteoclast differentiation by inhibiting RANKL-induced osteoclastogenic genes expression through the p38 MAPK signaling pathways. These results suggest that TME and its bioactive components are potential therapeutics for bone-related diseases such as osteoporosis.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"226 1","pages":"983-989"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78473974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Recent Trends on Telomerase Activators, Sirtuin Activators, and Senolytics as a Potential Anti-aging Agent 端粒酶激活剂、Sirtuin激活剂和抗衰老药物的研究进展

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.9.819

Moon‐Moo Kim

{"title":"Recent Trends on Telomerase Activators, Sirtuin Activators, and Senolytics as a Potential Anti-aging Agent","authors":"Moon‐Moo Kim","doi":"10.5352/JLS.2020.30.9.819","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.9.819","url":null,"abstract":"All living organisms exhibit the characteristics of aging, such as skin wrinkle formation, muscle degeneration, cataracts, and hair graying as the number of aged cells increases over time. Senescence, which is known as a key cause of aging, is directly related to the aging of living organisms because cells are aged by external and internal factors and eventually cell proliferation is stopped. Senescence is caused by the gradual shortening of the telomere with cell division, and lifespan is determined by the length of the telomere. Recently, it has been found that the histone deacetylase, which can influence gene expression, is not only involved in yeast but also deeply involved in anti-aging mechanisms in both C. elegans and humans. It was also discovered that old cells play a decisive role in the aging phenomenon, and it has been reported that it is possible to promote the proliferation of young cells and delay aging by removing these senescent cells from the inside. Therefore, in order to develop potential anti-aging agents in the future, research should begin with an in-depth study of telomerase activators, sirtuin activators, and senolytics.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"23 1","pages":"819-825"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77986412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Understanding the Mechanisms of Particulate Matter-mediated Ocular Diseases 颗粒物质介导的眼部疾病机制研究进展

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.8.722

Hyesook Lee, Yung-Hyun Choi

{"title":"Recent Advances in Understanding the Mechanisms of Particulate Matter-mediated Ocular Diseases","authors":"Hyesook Lee, Yung-Hyun Choi","doi":"10.5352/JLS.2020.30.8.722","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.8.722","url":null,"abstract":"As one of the most serious health risk factors, air pollution can no longer be ignored. Particulate matter (PM) is an important and harmful component of air pollution that originates from a variety of sources. Numerous recent studies have linked PM to a range of conditions including cancer, cardiovascular, respiratory, and skin disease. The eye, despite being directly exposed to air pollution, has been investigated in very few of these studies. In this review, we describe the evidence from in vitro and in vivo studies, as well as epidemiological investigations, that supports the association between exposure to PM and the development of ocular conditions such as surface and retinal disease and glaucoma. Based on the results of previous studies, we suggest that PM exposure can lead to oxidative stress, inflammation, autophagy, and, ultimately, ocular surface disease. Nevertheless, almost no studies focus on ocular surface damage from PM while some epidemiological and clinical studies report on the posterior of the eye. However, the underlying pathological mechanisms in the posterior following PM exposure have yet to be identified, and further studies are therefore warranted of the ocular surface as well as the posterior part of the eye.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"73 1","pages":"722-730"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88685562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Heterosigma akashiwo를 모니터하기 위한 뉴클레아제 보호 분석이 통합된 샌드위치 혼성(NPA-SH)의 개발 一种结合了磷酸酶保护分析来监测Heterosigma akashiwo的三明治混合(NPA-SH)。

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.1.26

Mingyeong Kang, Mi-Ryong Park, K. Kim, Taek Kyun Lee

引用次数: 0

Renal Failure in a Female Muskrat 雌性麝鼠的肾功能衰竭

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.7.630

Ullah H. M. Arif, A. Elfadl, Park, Sun-young, Chung Myung-Jin, Son Ji-Yoon, Yun Hyun-Ho, Park, Jae-min, Yim Jae-Hyuk, Jung Seung-Jun, Park Jinkyu, Jeong Kyu-Shik

{"title":"Renal Failure in a Female Muskrat","authors":"Ullah H. M. Arif, A. Elfadl, Park, Sun-young, Chung Myung-Jin, Son Ji-Yoon, Yun Hyun-Ho, Park, Jae-min, Yim Jae-Hyuk, Jung Seung-Jun, Park Jinkyu, Jeong Kyu-Shik","doi":"10.5352/JLS.2020.30.7.630","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.7.630","url":null,"abstract":"Renal failure syndrome in wild mammals is infrequently reported. Muskrat (Ondatra zibethicus) is a medium-sized rodent known to carry many diseases but rarely exhibiting renal failure. A six-month old female muskrat was submitted to our laboratory for pathological diagnosis, and necropsy revealed severe renal damage with sand-like lithiasis in the ureter, renal calculi, and hydronephrosis. All major organs, including the cerebrum, also showed systemic hemorrhage and calcification which may have been due to uremia induced by renal failure. Histopathologically, necrosis and microcalcification were detected in the renal cortex and the medulla, especially in the proximal convoluted tubules and collecting ducts of the kidney. Significant hyalinization of the glomeruli was also observed, and this suggested chronic nephritis. These findings would support mycotoxic effects, particularly on the kidney. Moreover, infiltration of neutrophils and mononuclear cells was observed in the lung and of plasma cells in the spleen. The definitive cause of the toxic effects in this case of muskrat renal failure could be attributed to contaminated food.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"124 1","pages":"630-633"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91297199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α' Subunit Protein 不含库尼茨胰蛋白酶抑制剂、凝集素和7S α亚基蛋白的大豆新品系的选育

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.7.592

Won Gi Chae, S. Choi, Gyung Young Kang, J. Chung

{"title":"The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α' Subunit Protein","authors":"Won Gi Chae, S. Choi, Gyung Young Kang, J. Chung","doi":"10.5352/JLS.2020.30.7.592","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.7.592","url":null,"abstract":"Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α'subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI ᅦprotein was detected using Western blot while 7S α' subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α' subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain’s height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"1 1","pages":"592-597"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90365676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Response Surface Methodology in Medium Optimization to Improve Lactic Acid Production by Lactobacillus paracasei SRCM201474 响应面法在副干酪乳杆菌SRCM201474培养基优化中的应用

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.6.522

Gwangsu Ha, Kim Jin Won, Sua Im, Su-Jin Shin, Hee-Jong Yang, Do-Youn Jeong

{"title":"Application of Response Surface Methodology in Medium Optimization to Improve Lactic Acid Production by Lactobacillus paracasei SRCM201474","authors":"Gwangsu Ha, Kim Jin Won, Sua Im, Su-Jin Shin, Hee-Jong Yang, Do-Youn Jeong","doi":"10.5352/JLS.2020.30.6.522","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.6.522","url":null,"abstract":"The aim of this study was to establish the optimal medium composition for enhancing L(+)-lactic acid (LLA) production using response surface methodology (RSM). Lactobacillus paracasei SRCM201474 was selected as the LLA producer by productivity analysis from nine candidates isolated from kimchi and identified by 16S rRNA gene sequencing. Plackett-Burman design was used to assess the effect of eleven media components on LLA production, including carbon (glucose, sucrose, molasses), nitrogen (yeast extract, peptone, tryptone, beef extract), and mineral (NaCl, K2HPO4, MgSO4, MnSO4) materials. Glucose, sucrose, molasses, and peptone were subsequently chosen as promising media for further optimization studies, and a hybrid design experiment was used to establish their optimal concentrations as glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, and peptone 34.91 g/l. The coefficient of determination of the equation derived from RSM regression for LLA production was mathematically reliable at 0.9969. At optimum parameters, 33.38 g/l of maximum LLA increased by 193% when compared with MRS broth as unoptimized medium (17.66 g/l). Our statistical model was confirmed by subsequent validation experiments. Increasing the performance of LLA-producing microorganisms and establishing an effective LLA fermentation process can be of particular benefit for bioplastic technologies and industrial applications.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"25 1","pages":"522-531"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74540791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0