Journal of Life Science最新文献_第5页

LC-MS/MS Screening Method for Radical Scavenging Active Compounds in Extracts of Ulmus pumila Cortex 榆皮提取物清除自由基活性物质的LC-MS/MS筛选方法

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.11.956

D. Im, K. Lee

引用次数: 0

Immunomodulatory Effects of β-sitosterol and Daucosterol Isolated from Dioscorea batatas on LPS-stimulated RAW 264.7 and TK-1 Cells 薯蓣中β-谷甾醇和大豆甾醇对lps刺激的RAW 264.7和TK-1细胞的免疫调节作用

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.4.359

Min-Kyung Park, Se-hee Cho, Tae-kyu Ahn, Do Hyun Kim, Soyeon Kim, Jinwook Lee, Jee-in Kim, E. Seo, K. Son, Jae-hwan Lim

引用次数: 1

Anti-inflammatory Activity of Antimicrobial Peptide Papiliocin 3 Derived from the Swallowtail Butterfly, Papilio xuthus 从燕尾蝶中提取的抗菌肽Papiliocin 3的抗炎活性

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.10.886

Yong pyo Shin, Joon-Ha Lee, In-woo Kim, M. Seo, Mi-Ae Kim, H. Lee, Minhee Baek, Seong Hyun Kim, Jae‐Sam Hwang

{"title":"Anti-inflammatory Activity of Antimicrobial Peptide Papiliocin 3 Derived from the Swallowtail Butterfly, Papilio xuthus","authors":"Yong pyo Shin, Joon-Ha Lee, In-woo Kim, M. Seo, Mi-Ae Kim, H. Lee, Minhee Baek, Seong Hyun Kim, Jae‐Sam Hwang","doi":"10.5352/JLS.2020.30.10.886","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.10.886","url":null,"abstract":"The development of novel peptide antibiotics with potent antimicrobial activity and anti-inflammatory activity is urgently needed. In a previous work, we performed an in-silico analysis of the Papilio xuthus transcriptome to identify putative antimicrobial peptides and identified several candidates. In this study, we investigated the antibacterial and anti-inflammatory activities of papiliocin 3, which was selected bioinformatically based on its physicochemical properties against bacteria and mouse macrophage Raw264.7 cells. Papiliocin 3 showed antibacterial activities against E. coli and S. aureus without inducing hemolysis and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Moreover, ELISA and Western blot analysis revealed that papiliocin 3 reduced the expression levels of pro-inflammatory enzymes, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). In addition, we examined whether papiliocin 3 could inhibit the expression of pro-inflammatory cytokines (interleukin-6 and interleukin-1β) in LPSinduced Raw264.7 cells. We found that papiliocin 3 markedly reduced the expression level of cytokines through the regulation of mitogen-activated protein kinases (MAPK) and nuclear factor kappa B (NF-κB) signaling. We also confirmed that papiliocin 3 binds to bacterial cell membranes via a specific interaction with lipopolysaccharides. Collectively, these findings suggest that papiliocin 3 could be a promising molecule for development as a novel peptide antibiotic.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"139 1","pages":"886-895"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80533718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial, Antioxidant, and Anti-diabetic Activities of Rodgersia podophylla 黑檀的抗菌、抗氧化和抗糖尿病活性

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.3.298

Su-Jin Pyo, Yun-jin Lee, Deok-Gyeong Kang, Ho-Jun Son, G. Park, Jong-Yi Park, H. Sohn

引用次数: 1

UV-induced Photodamage - attenuating Properties of Water Extract from Lentinuls edodes 香菇水提物对紫外光致光损伤的衰减特性

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.10.877

J. Lee, J. Oh, Soyoung Park, Hye-Ran Kim, K. Jung, Byung-Jin Jeon, Dong-Min Kim, C. Kong

引用次数: 1

Assessing the Use of 5 ml Straws in the Cryopreservation of Boar Semen 5ml吸管在猪精液冷冻保存中的应用评估

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.1.77

BeomYong Kim, Hyung-Bin Ham, Sang-Hyeon Kim, J. Son, K. Chung

引用次数: 0

Comparison and Validation of Genetic Diversity and Population Structure Using Monomorphic SNP Data of the Korean Native Black Goat and Crossbred Goat 利用韩国本土黑山羊和杂交山羊单态SNP数据进行遗传多样性和群体结构的比较与验证

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.11.1007

Kwan-Woo Kim, Jinwook Lee, Eun-Do Lee, Sung-Soo Lee, You-Lim Choi, Hyunsun Lim, Yousam Kim, Sang-Hoon Lee

引用次数: 0

Comparative Analysis of Nutritional Components of Zophobas atratus Larvae Raised with Artificial Diet and Wheat Bran 人工饲粮与麦麸饲养的狐尾虫幼虫营养成分的比较分析

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.12.1109

Sunyoung Kim, Kyu‐Won Kwak, K. Lee, Hyeon-Jin Ko, Yong‐Soon Kim, Eun Sun Kim, K. Park, H. Yoon

引用次数: 2

The Potential Probiotic and Functional Health Effects of Lactic Acid Bacteria Isolated from Traditional Korean Fermented Foods 韩国传统发酵食品中乳酸菌的潜在益生菌和功能性保健作用

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.7.581

J. Ohn, Min-Kyeong Seol, E. Bae, Young-je Cho, H. Jung, Byung-Oh Kim

{"title":"The Potential Probiotic and Functional Health Effects of Lactic Acid Bacteria Isolated from Traditional Korean Fermented Foods","authors":"J. Ohn, Min-Kyeong Seol, E. Bae, Young-je Cho, H. Jung, Byung-Oh Kim","doi":"10.5352/JLS.2020.30.7.581","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.7.581","url":null,"abstract":"This study investigated the probiotic properties and physiological activities of Korean fermented foods such as sikhae, young radish kimchi, and bean-curd dregs. Among the isolated lactic acid bacteria, Pediococcus inopinatus BZ4, Lactobacillus plantarum SH1, Lactobacillus brevis SH14, Pediococcus pentosaceus YMT1, and Leuconostoc mesenteroides YMT6 demonstrated a greater than 60% survival rate at pH 2.5, along with an excellent survival rate even at 0.3% bile acid. These five bacteria showed strong flocculation ability in autoaggregation and coaggregation tests, indirectly clustering useful micro-organisms and inhibiting the attachment of pathogenic bacteria. In a cell surface hydrophobicity test, these bacteria showed adhesion to three solvents (ethyl acetate, chloroform, and xylene) and high hydrophobicity, thereby indicating excellent indirect cell adhesion to intestinal cells. The cell-free supernatants and intracellular extracts of the five lactic acid bacteria showed antioxidative activity in the form of 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability and lipid peroxidation inhibition. Antimicrobial activities were also observed in four pathogenic bacteria, namely E. coli KCTC 2571, H. pylori HPKCTC B0150, L. monocytogenes KCTC 13064, and S. aureus KCTC 1916. These results demonstrate that these five lactic acid bacteria could be used as probiotics with antioxidant and antimicrobial properties.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"54 34 1","pages":"581-591"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88465935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Adjuvant Effect of PAMAM Dendrimer on the Antigenicity of Keyhole Limpet Hemocyanin in Balb/c Mice PAMAM树状大分子对Balb/c小鼠锁孔帽贝血青素抗原性的佐剂作用

Journal of Life Science Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.10.905

Ga-Young Lee, Min Jee Kim, So Yeon Kim, K. Lee, D. Oh, Y. Cho, Y. Yoo

{"title":"Adjuvant Effect of PAMAM Dendrimer on the Antigenicity of Keyhole Limpet Hemocyanin in Balb/c Mice","authors":"Ga-Young Lee, Min Jee Kim, So Yeon Kim, K. Lee, D. Oh, Y. Cho, Y. Yoo","doi":"10.5352/JLS.2020.30.10.905","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.10.905","url":null,"abstract":"The adjuvant effect of PAMAM dendrimer G4 (PAMAM) on the induction of humoral and cellular immune responses against keyhole limpet hemocyanin (KLH) was examined. Mice were immunized subcutaneously twice at two-week intervals with KLH, with or without PAMAM dendrimer (100 μ g/mouse), and the mice immunized with KLH+PAMAM showed significantly higher antibody titers against KLH than those immunized with KLH alone. The assay for determining the isotypes of the antibodies showed that PAMAM augmented the KLH-specific antibody titers of IgG1, IgG2a, IgG2b, IgG3, and IgM. In addition, mice immunized twice with KLH+PAMAM followed by a subcutaneous injection of KLH (20 μg/site) 7 weeks after the primary immunization exhibited a higher delayed-type hypersensitivity (DTH) reaction than those treated with KLH alone. In an in vitro analysis of T lymphocyte proliferation in response to KLH in week 8, the splenocytes of mice treated with KLH+ PAMAM showed significantly higher proliferating activity than those treated with KLH alone, and the culture supernatants of cell cultures from mice immunized with added PAMAM dendrimer showed higher levels of KLH-specific cytokine (IL-4 and IFN-r) production. These results suggest that PAMAM dendrimer G4 possesses a potent immune-adjuvant activity for enhancing both humoral and cell-mediated immunity specific to foreign antigens.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"10 1","pages":"905-911"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83723225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0