Journal of Life Science最新文献_第2页

Analysis of Cadaverine and Its Worker Honeybee Venom Content (Apis mellifera L.) 尸胺及其工蜂毒液含量分析(Apis mellifera L.)

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.144

H. Choi, H. Kim, S. Kim, S. Han

{"title":"Analysis of Cadaverine and Its Worker Honeybee Venom Content (Apis mellifera L.)","authors":"H. Choi, H. Kim, S. Kim, S. Han","doi":"10.5352/JLS.2021.31.2.144","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.144","url":null,"abstract":"This study aimed to analyze the content and composition of a biological amine, cadaverine, isolated from the venom of worker honeybees (Apis mellifera L.). This biological amine—which has diverse functionality, such as anti-inflammatory and antibacterial effects—has not been previously reported in bee venom. An assay completed in 13 minutes was developed for the cadaverine present in the bee venom using an ultra-performance liquid chromatograph and a Halo C18 column with acetonitrile and water as the mobile phase. The specificity, accuracy, and precision of the assay were verified, and the assay was validated. The linearity for cadaverine in the bee venom was R=0.99 or above, indicating a moderate level. The limit of detection and limit of quantification were both 0.3 μg/ml, and the rate of recovery was 97.6%-99.1%. The relative standard deviation (RSD) of the intra-day precision and inter-day precision for cadaverine was 0.25%-0.44% and 0.25%-1.25%, respectively, with an RSD that fell within 5% indicating excellent precision. Through this novel assay, it was found that the mean content of cadaverine was 1.10±0.05 mg/g. Our results indicated that the linearity, limit of detection, limit of quantification, and rate of recovery of the cadaverine assay were of a satisfactory level, and the cadaverine content of the bee venom was ably determined. This study provides basic data on cadaverine in bee venom, which will prove useful in further studies on the bioactivity of this component.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"17 1","pages":"144-148"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75342012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions 盐、冷、干旱胁迫下水稻OsCPK11 ND0001突变体的表达分析

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.115

Hyeon-Mi Kim, Sung-Ha Kim

{"title":"Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions","authors":"Hyeon-Mi Kim, Sung-Ha Kim","doi":"10.5352/JLS.2021.31.2.115","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.115","url":null,"abstract":"Calcium-dependent protein kinases (CDPKs) are known to be involved in regulating plant responses to abiotic stresses such as salinity, cold temperature and dehydration,. Although CDPKs constitute a large multigene family consisting of 31 genes in rice, only a few rice CDPKs’ functions have been identified. Therefore, in order to elucidate the functions of OsCPK11 in rice, this study was intended to focus on the expression pattern analysis of OsCPK11 in wild type and ND0001 oscpk11 mutant plants under these abiotic stresses. For the salt, cold and drought stress treatment, seedlings were exposed to 200 mM NaCl, 4°C and 20% PEG 6,000, respectively. RT-PCR and quantitative real-time PCR were performed to determine the expression patterns of OsCPK11 in wild type and ND0001 mutant plants. RT-PCR results showed that OsCPK11 transcripts in the wild type and heterozygous mutant were detected, but not in the homozygous mutant. Real-time PCR results showed that relative expression of OsCPK11 of wild type plants was increased and reached to the highest level at 24 hr, at 6 hr and at 24 hr under salt, cold and drought stress conditions, respectively. Relative expression of OsCPK11 of ND0001 homozygous plant was significantly reduced compared to that of wild type. These results suggested that oscpk11 homozygous mutant knocks out OsCPK11 and OsCPK11 might be involved in salt, cold and drought stress signaling by regulating its gene expression.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"48 1","pages":"115-125"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87978661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Undaria pinnatifida Extracts and Alginic Acid Attenuated Muscle Atrophy in TNF-α Induced Myoblast Cells through MAFbx Signaling Cascade 裙带菜提取物和褐藻酸通过MAFbx信号级联减轻TNF-α诱导的成肌细胞的肌肉萎缩

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.137

S. Choi, Mina Kim, H. H. L. Lee, J. Hur

{"title":"Undaria pinnatifida Extracts and Alginic Acid Attenuated Muscle Atrophy in TNF-α Induced Myoblast Cells through MAFbx Signaling Cascade","authors":"S. Choi, Mina Kim, H. H. L. Lee, J. Hur","doi":"10.5352/JLS.2021.31.2.137","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.137","url":null,"abstract":"Muscle atrophy refers to a decrease in muscle cells due to damage to muscle fibers. It is reported that muscle atrophy is caused by heart disease, diabetes, and other chronic diseases related to aging. The purpose of this study is to reveal the inhibitory effects of seaweed extracts, which are widely consumed in Korea, and alginic acid on muscle cell damage in muscle atrophy and regeneration models. We found that seaweed extracts (U) and alginic acid (A) attenuated TNF-α-induced muscle atrophy in differentiated C2C12 myoblast cells and inhibited muscle atrophy markers such as MuRF1 and MAFbx. In addition, U and A also regulated ubiquitination marker FoxO1 protein. To confirm the muscle regeneration effect in animal tissue, cardiotoxin (CTX) was used for the regeneration model. Six hours after CTX injection, gastrocnemius muscle volume was increased compared to control. Otherwise, the muscle volume of the U and A treatment groups was not changed. U and A also upregulated regeneration markers MyHC and PGC-1α in a CTX mouse model. These results indicate that seaweed extracts and alginic acid, a seaweed component, are applicable to senile sarcopenia by inhibiting muscle loss and promoting muscle regeneration.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"14 1","pages":"137-143"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81256040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant and Anticancer Activities of Euonymus porphyreus Extract in Human Lung Cancer Cells A549 卟啉卫矛提取物对人肺癌细胞A549的抗氧化和抗癌活性

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.199

Soojung Jin, Y. Oh, Y. Son, Soobin Bae, Jung-Ha Park, B. W. Kim, H. Kwon

引用次数: 0

The Antioxidant and Skin-whitening Effects of Saccharomyces cerevisiae FT4-4 Isolated from Berries Grown in Sunchang 孙昌地区浆果中酿酒酵母菌FT4-4的抗氧化和美白作用

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.175

J. Seo, M. Ryu, Hee-Jong Yang, Su-Ji Jeong, Do-Youn Jeong

引用次数: 2

Fucoidan Stimulates Glucose Uptake via the PI3K/AMPK Pathway and Increases Insulin Sensitivity in 3T3-L1 Adipocytes 岩藻糖聚糖通过PI3K/AMPK途径刺激葡萄糖摄取并增加3T3-L1脂肪细胞的胰岛素敏感性

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.1.1

Ji Hee Lee, J. E. Park, J. S. Han