Journal of Life Science最新文献_第2页

Ulmus macrocarpa Hance Reduces Cyclophosphamide-induced Toxicity in Mouse Liver 大榆可降低环磷酰胺对小鼠肝脏的毒性

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.223

D. Kim, K. Chung

{"title":"Ulmus macrocarpa Hance Reduces Cyclophosphamide-induced Toxicity in Mouse Liver","authors":"D. Kim, K. Chung","doi":"10.5352/JLS.2021.31.2.223","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.223","url":null,"abstract":"Cyclophosphamide (CP) is widely used in cancer and lymphoma treatments and as an immunosuppressant drug. CP is a DNA alkylating agent that metabolizes into 4-hydrocyclophosphamide (4HCYP) and aldophosphamide in hepatocytes. However, its metabolites cause DNA synthesis disorder, leading to apoptosis and toxic side effects. The development of technology to minimize this side effect is essential to improve CP’s clinical application. Various bioactive compounds have been reported to have anti-cancer and antioxidant functions and preventive or therapeutic roles in metabolic diseases. Many researchers have attempted to minimize the side effects and improve the efficacy of these drugs together with the use of bioactive compounds. Ulmus macrocarpa Hance has been used for the treatment of edema, mastitis, stomach pain, tumors, cystitis, and other inflammatory diseases. The aim of this study was to investigate at the histological level the protective function of U. macrocarpa Hance against CP’s side effects and any potential toxic effect of U. macrocarpa Hance in the liver and kidney. Water extracts of U. macrocarpa Hance reduced CP-induced toxicity and did not induce any histological damage in the liver and kidney. Therefore, U. macrocarpa Hance would be applicable in the pharmaceutical industry.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"10 3","pages":"223-228"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91438188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions 盐、冷、干旱胁迫下水稻OsCPK11 ND0001突变体的表达分析

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.115

Hyeon-Mi Kim, Sung-Ha Kim

{"title":"Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions","authors":"Hyeon-Mi Kim, Sung-Ha Kim","doi":"10.5352/JLS.2021.31.2.115","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.115","url":null,"abstract":"Calcium-dependent protein kinases (CDPKs) are known to be involved in regulating plant responses to abiotic stresses such as salinity, cold temperature and dehydration,. Although CDPKs constitute a large multigene family consisting of 31 genes in rice, only a few rice CDPKs’ functions have been identified. Therefore, in order to elucidate the functions of OsCPK11 in rice, this study was intended to focus on the expression pattern analysis of OsCPK11 in wild type and ND0001 oscpk11 mutant plants under these abiotic stresses. For the salt, cold and drought stress treatment, seedlings were exposed to 200 mM NaCl, 4°C and 20% PEG 6,000, respectively. RT-PCR and quantitative real-time PCR were performed to determine the expression patterns of OsCPK11 in wild type and ND0001 mutant plants. RT-PCR results showed that OsCPK11 transcripts in the wild type and heterozygous mutant were detected, but not in the homozygous mutant. Real-time PCR results showed that relative expression of OsCPK11 of wild type plants was increased and reached to the highest level at 24 hr, at 6 hr and at 24 hr under salt, cold and drought stress conditions, respectively. Relative expression of OsCPK11 of ND0001 homozygous plant was significantly reduced compared to that of wild type. These results suggested that oscpk11 homozygous mutant knocks out OsCPK11 and OsCPK11 might be involved in salt, cold and drought stress signaling by regulating its gene expression.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"48 1","pages":"115-125"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87978661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Undaria pinnatifida Extracts and Alginic Acid Attenuated Muscle Atrophy in TNF-α Induced Myoblast Cells through MAFbx Signaling Cascade 裙带菜提取物和褐藻酸通过MAFbx信号级联减轻TNF-α诱导的成肌细胞的肌肉萎缩

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.137

S. Choi, Mina Kim, H. H. L. Lee, J. Hur

{"title":"Undaria pinnatifida Extracts and Alginic Acid Attenuated Muscle Atrophy in TNF-α Induced Myoblast Cells through MAFbx Signaling Cascade","authors":"S. Choi, Mina Kim, H. H. L. Lee, J. Hur","doi":"10.5352/JLS.2021.31.2.137","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.137","url":null,"abstract":"Muscle atrophy refers to a decrease in muscle cells due to damage to muscle fibers. It is reported that muscle atrophy is caused by heart disease, diabetes, and other chronic diseases related to aging. The purpose of this study is to reveal the inhibitory effects of seaweed extracts, which are widely consumed in Korea, and alginic acid on muscle cell damage in muscle atrophy and regeneration models. We found that seaweed extracts (U) and alginic acid (A) attenuated TNF-α-induced muscle atrophy in differentiated C2C12 myoblast cells and inhibited muscle atrophy markers such as MuRF1 and MAFbx. In addition, U and A also regulated ubiquitination marker FoxO1 protein. To confirm the muscle regeneration effect in animal tissue, cardiotoxin (CTX) was used for the regeneration model. Six hours after CTX injection, gastrocnemius muscle volume was increased compared to control. Otherwise, the muscle volume of the U and A treatment groups was not changed. U and A also upregulated regeneration markers MyHC and PGC-1α in a CTX mouse model. These results indicate that seaweed extracts and alginic acid, a seaweed component, are applicable to senile sarcopenia by inhibiting muscle loss and promoting muscle regeneration.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"14 1","pages":"137-143"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81256040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant and Anticancer Activities of Euonymus porphyreus Extract in Human Lung Cancer Cells A549 卟啉卫矛提取物对人肺癌细胞A549的抗氧化和抗癌活性

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.199

Soojung Jin, Y. Oh, Y. Son, Soobin Bae, Jung-Ha Park, B. W. Kim, H. Kwon

引用次数: 0

The Antioxidant and Skin-whitening Effects of Saccharomyces cerevisiae FT4-4 Isolated from Berries Grown in Sunchang 孙昌地区浆果中酿酒酵母菌FT4-4的抗氧化和美白作用

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.2.175

J. Seo, M. Ryu, Hee-Jong Yang, Su-Ji Jeong, Do-Youn Jeong

引用次数: 2

Fucoidan Stimulates Glucose Uptake via the PI3K/AMPK Pathway and Increases Insulin Sensitivity in 3T3-L1 Adipocytes 岩藻糖聚糖通过PI3K/AMPK途径刺激葡萄糖摄取并增加3T3-L1脂肪细胞的胰岛素敏感性

Journal of Life Science Pub Date : 2021-01-01 DOI: 10.5352/JLS.2021.31.1.1

Ji Hee Lee, J. E. Park, J. S. Han