Journal of Mass Spectrometry最新文献

{"title":"Reverse-Phase High-Performance Liquid Chromatography/Mass Spectrometry (RP-LC–MS/MS, Label-Free Method) for Component Analysis of Gonadotropin Drugs","authors":"Daomiao Huang,&nbsp;Hong Chen,&nbsp;Hai Zhang,&nbsp;Xin Dong","doi":"10.1002/jms.70057","DOIUrl":"10.1002/jms.70057","url":null,"abstract":"<div>\u0000 \u0000 <p>In assisted reproductive technology (ART), controlled ovarian hyperstimulation (COS) plays an important role in guiding treatment progress and determining success. Many ovulation-inducing agents are used in this process, including human menopausal gonadotropin (HMG), recombinant follicle-stimulating hormone (r-FSH), recombinant luteinizing hormone (r-hLH), urinary human chorionic gonadotropin (u-hCG), and recombinant human chorionic gonadotropin (r-hCG). HMG, which comes from the urine of postmenopausal women, mainly contains FSH, LH, and hCG. While bioactivity-based potency units and immunoassay-based ELISA methods remain the gold standard for quantifying protein therapeutics, LC–MS/MS is emerging as a game-changing technology. Its unique strengths are exceptional specificity and absolute quantification capabilities, independence from specialized reagents, powerful multiplexing potential, and ability to provide structural insights, establishing it as an essential tool for both orthogonal verification and cutting-edge research in the field. This study used reverse-phase liquid chromatography with mass spectrometry (RP-LC–MS/MS) and a label-free quantitative proteomics method to analyze imported r-hFSH, r-hLH, r-hCG (Gonal-F, Luveris, and Ovidrel), three domestic HMG products (Le Baode, Man Fuxin, and Xin Yunle), and u-hCG (Li Zhu). The results showed that the purity of the main component in imported gonadotropin drugs was higher than 98.55%. On the other hand, the main element of domestic u-HMG is FSH, with small amounts of hCG and very little LH. These results reveal significant differences in the composition of imported and domestic gonadotropin products, underscoring the need to further evaluate their biological activity and clinical effectiveness. This study provides important insights into drug selection for ART, with practical value for improving the quality of HMG drugs and making better use of them in clinical settings.</p>\u0000 </div>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148069801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput N-Glycan Analysis by IR-MALDESI With a 1.5-kHz Pulsed Mid-IR Laser","authors":"Seth M. Eisenberg,&nbsp;Sarah M. Ashbacher,&nbsp;Alexander A. C. Wainwright,&nbsp;Aosheng Gu,&nbsp;R. J. Dwayne Miller,&nbsp;David C. Muddiman","doi":"10.1002/jms.70056","DOIUrl":"10.1002/jms.70056","url":null,"abstract":"<p>Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is a laser-based, hybrid ionization source for mass spectrometry, enabling both mass spectrometry imaging (MSI) and high-throughput screening (HTS). In this work, a 2.2-ns, 2.72-μm laser, operating at 1.5 kHz, was integrated into the IR-MALDESI platform to evaluate its performance for robust HTS and glycan profiling. The system produced robust HTS performance, achieving a relative standard deviation (RSD) of 9.1% with no detectable carryover between wells and consistent ion abundances in multiwell screening experiments. Using this configuration, 29 glycans were detected from a solution of cleaved <i>N</i>-glycans derived from bovine fetuin, with multiple charge states observed for several species. These results demonstrate rapid, high-throughput analysis and expanded glycan detection enabled by desorption using a low-power mid-IR laser operating at kHz repetition rates, highlighting its potential for advanced biochemical screening and characterization.</p>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148066921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148066920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a Top-Down Liquid Chromatography-High-Resolution-Mass Spectrometry Method With Multiple Fragmentation Modes for Clinical Diagnosis of Hemoglobin Variants","authors":"Danya Ortiz,&nbsp;Farida Elgebaly,&nbsp;Ryan M. Aquino,&nbsp;Hector A. Vidal,&nbsp;Carolyn V. Wong,&nbsp;Ruben Y. Luo","doi":"10.1002/jms.70054","DOIUrl":"10.1002/jms.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Identification of hemoglobin (Hb) variants is of significant value in the clinical diagnosis of hemoglobinopathies. A common issue with the conventional methods for identifying Hb variants is their limited resolution to provide primary structures. Previous literature has shown the ability for high-resolution mass spectrometry (HR-MS) methods to accurately identify Hb variants, but ambiguity may still remain when distinguishing isomeric Hb variants due to inadequate AA sequence coverage. Using multiple fragmentation modes can be a solution to achieve higher sequence coverage in MS² fragment analysis. A liquid chromatography-high-resolution mass spectrometry (LC-HR-MS) method with multiple fragmentation modes was developed for identifying Hb variants, which could both effectively separate pairs of normal and variant Hb subunits and accurately distinguish isomeric Hb variant subunits. The separation was facilitated by a C4 reversed-phase column, and the fragmentation modes in use were higher-energy collision dissociation (HCD) and electron-transfer/higher-energy collision dissociation (EThcD). The fragmentation modes HCD, ETD, and EThcD were tested with normal α and β subunits to evaluate the MS² performance of each mode. The combined use of EThcD and HCD was able to make high AA sequence coverage. Five clinical samples, including heterozygous Hb E and Hb Constant Spring (CS), Hb Hofu, Hb Raleigh, Hb P-Nilotic, and Hb F-Kuala Lumpur (KL), were analyzed to demonstrate the performance of the LC-HR-MS method for accurately identifying Hb variants samples. This article demonstrated the advantages of LC-HR-MS with multiple fragmentation modes in effectively solving clinical cases of Hb variants. In clinical practice, the combination of the LC-HR-MS and conventional Hb test methods is favorable, allowing for accurate diagnosis of Hb variants and significantly reducing the need for more costly genetic tests.</p>\u0000 </div>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148099796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to ‘Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry Method Development and Validation to Quantify Simultaneously Six Urinary Dialkyl Phosphate Metabolites of Organophosphorus Pesticides’","authors":"","doi":"10.1002/jms.70042","DOIUrl":"10.1002/jms.70042","url":null,"abstract":"<p>We apologize for these errors.</p>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jms.70042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148099795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"61 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148126837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}