{"title":"Advancing Native Mass Spectrometry Toward Cellular Biology","authors":"Kazumi Saikusa","doi":"10.1002/jms.5095","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Protein structure, including various post-translational modifications and higher-order structures, regulates diverse biological functions. Native mass spectrometry (native MS) is a powerful analytical technique used to determine the masses of biomolecules, such as proteins and their complexes, while preserving their native folding in solution. This method provides structural information on the composition of monomers or complexes and the stoichiometry of subunits within each complex, significantly contributing to protein structural analysis. Native MS has evolved to incorporate top–down approaches, enabling the characterization of proteoforms and non-covalent interactions between metabolites or proteins and specific targets. This perspective highlights the advancements in native MS for intracellular proteins and protein complexes, and discusses future research directions toward cellular biology.</p>\n </div>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"59 11","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jms.5095","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Protein structure, including various post-translational modifications and higher-order structures, regulates diverse biological functions. Native mass spectrometry (native MS) is a powerful analytical technique used to determine the masses of biomolecules, such as proteins and their complexes, while preserving their native folding in solution. This method provides structural information on the composition of monomers or complexes and the stoichiometry of subunits within each complex, significantly contributing to protein structural analysis. Native MS has evolved to incorporate top–down approaches, enabling the characterization of proteoforms and non-covalent interactions between metabolites or proteins and specific targets. This perspective highlights the advancements in native MS for intracellular proteins and protein complexes, and discusses future research directions toward cellular biology.
蛋白质结构,包括各种翻译后修饰和高阶结构,调节着各种生物功能。原生质谱(native MS)是一种功能强大的分析技术,用于测定蛋白质及其复合物等生物大分子的质量,同时保留其在溶液中的原生折叠。这种方法可提供有关单体或复合物组成的结构信息,以及每个复合物内亚基的化学计量学信息,对蛋白质结构分析大有帮助。原位质谱已发展成为一种自上而下的方法,可鉴定蛋白质形态以及代谢物或蛋白质与特定目标之间的非共价相互作用。本视角重点介绍了本机 MS 在细胞内蛋白质和蛋白质复合物方面的进展,并讨论了细胞生物学的未来研究方向。
期刊介绍:
The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions.
The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.