journal of ilam university of medical sciences最新文献_第9页

Evaluation of Antibacterial Properties of Magnetic Iron Oxide Nanoparticles Synthesized using Echinops Persicus Extract Coated with Chloramphenicol 氯霉素包被猕猴桃提取物制备磁性氧化铁纳米颗粒的抗菌性能评价

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.59

Reihaneh Ahmadieh, S. Mohseni

引用次数: 0

Identification of a Suitable Place for Sanitary Landfill in Ilam 伊拉姆地区卫生填埋场适宜选址的确定

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.38

Mohamad Feizi, A. Behbahaninia, Saeed Reza Asemi Zavareh, N. Rostami

引用次数: 0

Effectiveness of Mindfulness Training on EMotional Dysfunction and Academic Achievement of Junior Middle School Students 正念训练对初中生情绪障碍和学业成绩的影响

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.100

Meysam Mohebi, Majid Zargham hajebi, Nader Monirpoor

引用次数: 0

Investigation of the Effects of Vitamin C on Resistance to 5-FU in Colon Cancer Cells Line HT29 维生素C对结肠癌细胞HT29对5-FU耐药性影响的研究

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.49

A. Zarei, S. Ghorbian

引用次数: 0

A Survey on the Observance rate of Research Ethics Principles in Approved Proposals at Ilam University of Medical Sciences, Ilam, Iran 伊朗伊拉姆医科大学批准提案中研究伦理原则遵守情况调查

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.12

A. Aivazi, M. Hoseiny-Rad, Mojtaba Parsa

引用次数: 0

Comparison of Antioxidant, Antibacterial, and Cytotoxic Effects of Essential Oil and Nanoemulsion of Clove Essential Oil 丁香精油与丁香精油纳米乳抗氧化、抗菌及细胞毒作用的比较

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.26

Atena Vafayi Malek, E. Karimi, E. Oskoueian

引用次数: 0

Effects of oleuropein on malondialdehyde level and expression of GluN2B gene following passive avoidance memory impairment in mice 橄榄苦苷对小鼠被动回避记忆损伤后丙二醛水平及GluN2B基因表达的影响

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.92

G. Mahmoodi, A. Moshfegh

引用次数: 0

Phytochemical Investigation, Antioxidant Properties, and Identification of Organic and Inorganic Compounds from the Carob (Ceratonia siliqua L.) Fruit 角豆(Ceratonia silqua L.)的植物化学研究、抗氧化特性及有机和无机化合物的鉴定水果

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.82

M. Nasiri, Babak Mokhtari, M. Kolahi, N. Pourreza

引用次数: 0

A Survey on General Health Status of Paddy Field Workers in Mazandaran Province using the GHQ-28 Questionnaire 用GHQ-28问卷调查马赞达兰省稻田工人一般健康状况

journal of ilam university of medical sciences Pub Date : 2021-09-01 DOI: 10.52547/sjimu.29.3.72

Mohsen Sharifirad, A. Poursaeed, F. Lashgarara, S. Mirdamadi

引用次数: 0

Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions 无内毒素条件下小鼠BMDC分离培养优化

journal of ilam university of medical sciences Pub Date : 2021-05-10 DOI: 10.52547/sjimu.29.2.43

Atefeh Sadeghi Shermeh, S. Habibzadeh, Adeleh Taghikhani, S. Rafati, N. Seyed

{"title":"Optimized Mouse BMDC Isolation and Culture under \u0000Endotoxin-Free Conditions","authors":"Atefeh Sadeghi Shermeh, S. Habibzadeh, Adeleh Taghikhani, S. Rafati, N. Seyed","doi":"10.52547/sjimu.29.2.43","DOIUrl":"https://doi.org/10.52547/sjimu.29.2.43","url":null,"abstract":"Introduction: Dendritic cells are very important in basic studies and vaccine research, but isolation and culture of these cells face challenges due to their small number in tissues. Since there is no standard method, we addressed some of the factors affecting the efficiency of dendritic cell isolation and culture from BALB/c mouse bone marrow. Materials & Methods: Bone marrow cells isolated from femur and tibia, were seeded in plates and treated with GM-CSF. On day 3, half of the media was transferred to a new plate with fresh media plus GM-CSF. On day 5, cells were induced with CpG oligonucleotide. Cell survival as well as maturation markers of CD11C, MHC-II and CD86 were investigated by flow cytometry on day 5 (immature cells) and day 7 (mature cells). Ethics code: IR.PII.REC.1395.99 Findings: It was found that the presence of endotoxin in the culture of immature cells, significantly reduces the recovery of dendritic cells (p value <0.05). Less than one million cells seeded in non-treated 6-well plates and transfer of cells to a new plate on day 3 increased the efficiency of immature cell retrieval by preventing early maturation and premature death. Further treatment of immature cells with CpG on day 7 was used to induce the Th1 pathway. Significant difference (p value <0.05) with control group showed that CpG induces maturation after treatment with GM-CSF (without IL4). Discussion & Conclusions: Optimization of dendritic cell culture in terms of different culture conditions highly impacts the efficiency of immature cell formation and maturation.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80866620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0