journal of ilam university of medical sciences最新文献

{"title":"Synthesis of PEG-PLA Block Copolymer Nanospheres Containing Mixed Recombinant Proteins of Protective Antigen and Lethal Factor of Bacillus anthracis","authors":"H. Honari, M. Minaei, Hassan Mirhaj, Sayyed Masih Etemadaubi","doi":"10.52547/sjimu.29.5.89","DOIUrl":"https://doi.org/10.52547/sjimu.29.5.89","url":null,"abstract":"Article type: Orginal article Introduction: Anthrax is a zoonotic disease and vaccine production is one way to protect people against this bacterium. This study investigates the application of copolymer nanocapsule (PEG-PLA) systems with controlled release specificity for a recombinant protective antigen and lethal factor of Bacillus anthracis to provide a vaccine candidate. Material & Methods: In this experimental study, the dual solvent evaporation emulsion method was used to produce nanocapsules. Moreover, zeta potential and size of nanoparticles, loading efficiency of nanoparticles, recombinant protein release pattern, the probable effect of PLA-PEG nanoparticle production on the shelf life of recombinant proteins were investigated in this study. Mice were used as test and control samples for antibody production and immune response evaluation. (Ethic code: 9727250) Findings: The results of this study showed that mixed proteins (LFD1+PAD4) loaded on PEG-PLA block copolymer had an average size of 109 nm, the zeta potential of -27.7 mV, and PDI=0.394. In this study, it was shown that the release of these antigens was carried out in two stages of rapid and slow release. The release of the proteins was estimated at about 20% on the first day and 78% on the 49th day. The titers of antibodies produced in the serum of the mouse groups against these antigens at defined intervals were significantly different from each other. Discussion & Conclusion: The results of this study suggest the application of PEG-PLA block copolymer nanocapsules containing mixed recombinant proteins of protective antigen and lethal factor of Bacillus anthracis. Due to less degradation and more protection of antigen activity in nanoparticles, compared to traditional methods, as well as fewer repeat injections, higher specificity, reduced side effects, lower cost, and slower release rate, the use of nanoparticles is a good option to replace traditional methods.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74978481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Apoptotic Genes after Autotransplantation of Vitrified Rat Ovary Encapsulated with Hyaluronic Acid Hydrogel","authors":"Maryam Akhavan Taheri, M. Rezazadeh Valojerdi, B. Ebrahimi","doi":"10.52547/sjimu.29.5.12","DOIUrl":"https://doi.org/10.52547/sjimu.29.5.12","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74588777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Anticancer Effects of Nanocomposite of the Modified Graphene Oxide with Isatin-3-Semicarbazone on the Retinoblastoma Cells (Y79) Invitro","authors":"Z. Ramezani, H. Tahermansouri, F. Kiani","doi":"10.52547/sjimu.29.5.75","DOIUrl":"https://doi.org/10.52547/sjimu.29.5.75","url":null,"abstract":"Article type: Orginal article Introduction: The most common ocular tumor is retinoblastoma, which can be treated by different methods. The use of carbon nanostructures has attracted much attention due to their unique properties, such as crossing cell membranes, for cancer diagnosis and other medical applications. This study aimed to investigate and assess the toxicity of the modified graphene oxide (GO) with isatin-3-semicarbazone (ISA) in order to treat retinoblastoma. Material & Methods: This study utilized ISA to modify the GO. Subsequently, the modified GO-ISA was characterized by different techniques, such as Fourier transform infrared spectrometry, Scanning Electron Microscope, and Thermal Gravimetric Analysis. The cell toxicity of samples was assessed by MTT assay for two types of cells of retinoblastoma (Y79) and adipose-derived stem cells (ADSC) after 24 h at different concentrations. Data were analyzed in SPSS software using one-way analysis of variance (ANOVA). A p-value less than 0.05 was considered statistically significant. Findings: The results showed that in the concentration range of 10-50 μL, GO-ISA decreased significantly (P<0.01) the conservation percentage of retinoblastoma cells from 73.98% to 27.48%, while these values for ADSC decreased from 78.16% to 50.11% (P<0.01). In addition, GO-ISA at 30 μL resulted in a significant increase (P<0.01) in the cell toxicity of the retinoblastoma (26%), compared to GO. Discussion & Conclusion: GO-ISA increases the toxicity of the retinoblastoma cells, compared to GO which was significant at 30 μL. Therefore, this modification of the surface can be useful in future applications. With this modification of the surface, the active sites are provided for the biological and pharmacogenetic reactions in future.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80443074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Antimicrobial Properties and Toxicity of Natural S3 Peptide with Horseshoe Crab Amoebocyte Origin and its Mutants","authors":"Sadegh Rezaei, S. Hadadian, R. A. Khavari nejad, D. Norouzian","doi":"10.52547/sjimu.29.4.60","DOIUrl":"https://doi.org/10.52547/sjimu.29.4.60","url":null,"abstract":"Introduction: Antimicrobial peptides (AMPs) are compounds with antimicrobial properties that are studied widely due to the development of resistance of pathogenic bacteria to antibiotics. In the present study, the toxicity and antimicrobial effects of two natural monomeric peptides (S3 and S∆3) were compared with S3-S∆3 hybrids and S3 tetramers. Material & Methods: Protein hybrids (S∆3S3-2mer-GS) S3-S∆3 and tetramer protein S3 (S3-4mer-GS) were expressed in E. coli . BL21 (DE3). Following that, the presence of mutant peptides was confirmed, and their antimicrobial activity was compared and evaluated with S3 and S∆3 monomers. Finally, the toxicity of tetramer and hybrid made on the MDA-MB-231 cell line was evaluated and compared. Findings: The toxicity of the hybrid was slightly increased, compared to the tetramer for eukaryotic cells; however, this increase was negligible at the active concentration of this protein. Cell survival for hybrids was lower for S3 and SΔ3; nonetheless, cell survival for each sequence decreased with increasing time. Furthermore, the inhibition of hybrid microbial growth was improved and compared with tetramer and S3-SΔ3. It was found that an increase in the positive charge of the hybrid protein did not have a toxic effect on the host bacteria. Discussion & Conclusion: Due to the appropriate expression and increased antimicrobial activity and negligible cytotoxicity, the hybrid peptide S3-S∆3 and tetramer S3 can be considered an effective production strategy to obtain AMPs.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82990288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Number of Root Canals in Mandibular Molars and Premolars Teeth in Ilam in 2020","authors":"Khadijeh Abdal, Parisa Bakhtizad, Amin Kheiri","doi":"10.52547/sjimu.29.5.56","DOIUrl":"https://doi.org/10.52547/sjimu.29.5.56","url":null,"abstract":"types of be at least one the some roots have multiple canal of different length. The goal of this study was investigation of the number of root canals in mandibular molars and premolars teeth in Ilam in 2020. Material & Methods: This study was descriptive-analytic study that performed on 60 mandibular premolars and molars (first premolars 15, second premolars 15, first molars 15, second molars 15) randomly with considering of the sex of patients. Data were analyzed by SPSS22. (Ethic Findings: In the first and second premolars, the highest frequency of single canal as 86.7% and 93.3%, respectively. In the first and second molars, the highest frequencies related to the three channels were 53.3% and 86.7%, respectively. The prevalence of 4-channel molars was higher in women and 3-channel in men. This difference was not statistically significant. Between two radiologists and endodontists to determine the number of root canals in the first and second premolars and the first and second molars, a good agreement was reported with kappa agreement coefficient of 0.92 and (P = 0.000). Conclusion: study, the and second mandibular premolars single root and one and the first and second mandibular molars had often two roots and three channels.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84912810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Royal Jelly on Sperm Parameters, In-vitro Fertilizing Potential, and Oxidative Stress Indicators in the Testis of Mice Exposed to Ethephon","authors":"Ramin Jahangirfard","doi":"10.52547/sjimu.29.4.18","DOIUrl":"https://doi.org/10.52547/sjimu.29.4.18","url":null,"abstract":"Introduction: Ethephon is an organophosphorus that used to stimulate plant growth. Royal Jelly is a substance with antioxidant properties and is effective for strengthening the immune system. This study aimed to determine the effect of Royal Jelly on sperm parameters, in vitro fertilization, and oxidative stress indicators in mice. Material & Methods: In this experimental study, 20 adult male mice (NMRI strain) were divided into four control and experimental groups. The experimental group received Ethephon at 480 mg/kg. Royal Jelly was then administered orally and daily at a concentration of 200 mg/kg alone and in combination with Ethephon. After treatment, sperm samples were collected from the tail of epididymis to investigate sperm parameters and in vitro fertilizing potential; moreover, the testis tissue was taken for oxidative stress examination. A p-value less than 0.05 was considered statistically significant. (Ethic code: IR-UU_AEC-811/ دپ /3) Findings: A decrease in the sperm motility and viability, fertilization rate, blastocyst, and hatching embryos, as well as an increase in DNA damage, lack of nuclear maturation, and blocked embryos was observed in the experimental groups, compared to the control group. Moreover, Total Antioxidant Capacity and Malondialdehyde in the groups receiving Ethephon decreased and increased, respectively. However, Royal Jelly can prevent the adverse effects of oxidative stress (P<0.05). Discussion & Conclusion: According to the results of this study, royal jelly can have an amelioration effect on epididymal sperm and in vitro fertilizing potential.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74538529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes","authors":"S. M. Mousavi, M. Batavani, M. Ghofrani","doi":"10.52547/sjimu.29.4.93","DOIUrl":"https://doi.org/10.52547/sjimu.29.4.93","url":null,"abstract":"Article type: Orginal article Introduction: The ACE gene encodes the angiotensin-converting enzyme (ACE), which is characterized by the presence of allele I or the absence of allele D of a 287-bp fragment. The D allele is associated with higher ACE activity and increased angiotensin II levels and performance in strength-oriented exercise tasks. This study aimed to compare the genetic profile of rs4646994 single nucleotide polymorphism of the ACE gene in the elite male weightlifters and nonathletes. Material & Methods: In total, 30 elite male weightlifters (league, country, national team, Asian, world, and Olympic champions) living in Isfahan province along with 43 healthy non-athlete volunteers who were matched in age, height, and weight to the elite group formed the subjects of the study. After completing the consent form, saliva samples were collected from the participants, and after DNA extraction, ACE genotypes were determined using PCR-RLFP and electrophoresis techniques. Data analysis was performed using independent t-test, chi-square, and logistic regression in SPSS software (version 20). (Ethic code: 7993) Findings: The results showed that the prevalence of D allele in elite weightlifters and non-athletes was significantly higher than allele I in their groups (P<0.05). Moreover, in the genotyping distribution, D/I was most common both in the study population (7.50%), as well as weightlifters (7.56%) and non-athletes (5.46%). However, there was no significant difference in the elite weightlifters and nonathletes regarding the distribution of D/I ACE genotyping (χ=0/31). Discussion & Conclusion: The study showed a positive relationship between Iranian elite weightlifters and the ACE D allele, which probably distinguishes it as one of the effective genetic factors in creating a strength phenotype of Iranian weightlifters.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89807454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Life Skill Trainings in Ego Power, Temperament, and Suicidal Thoughts among Girls and Boys Attempting to Commit Suicide in Ilam Province, Iran","authors":"Shahryar Mehrdadi, F. Hassani, Farnaz Keshavarzi, Mahdeh Salehi, Mozhgan Sepah Mansour","doi":"10.52547/sjimu.29.4.46","DOIUrl":"https://doi.org/10.52547/sjimu.29.4.46","url":null,"abstract":"Article type: Orginal article Introduction: Suicide is caused by the interaction of many factors; however, most suicides are preventable. If suicide does not lead to death, there is concern that the behavior may be repeated. Teaching life skills based on participatory and practical learning is very effective in increasing health and early prevention of problems, as well as helping adolescents to learn positive behaviors and maintain their mental health. This study aimed to compare the effectiveness of life skill training in ego power, temperament, and suicidal thoughts among the girls and boys who attempted to commit suicide in Ilam province, Iran. Material & Methods: This semi-experimental study was conducted based on a pretest-posttest design with control and follow-up groups. The statistical population of the research included all boys and girls aged 18 to 24 (n=212) in Ilam province who for the first time over the past six months in 2018-2019 committed suicide and were referred to the medical centers of this province. In total, 80 cases were purposefully selected as an accessible sample and were matched and assigned into (based on education status) the experimental (20 girls and 20 boys) and control groups (20 girls and 20 boys). The data were collected using the temperament scale, ego scale, and suicidal thought scale. The data were analyzed through variance analysis with frequent measurements. (Ethic code:10120705972003) Findings: The results of analysis of variance showed a significant difference in prepost life skill training test among girls and boys who attempted to commit suicide in Ilam province in the experimental, control, and follow-up groups in terms of the level of temperament, ego power, and suicidal thoughts. Similarly, multivariate analysis of variance showed that life skill training in group type and test status had an effect on temperament and its dimensions, ego power, and suicidal thoughts of boys and girls who attempted to commit suicide in Ilam province. Discussion & Conclusion: According to the findings it can be concluded that life skills training has been effective in ego power, temperament, and suicidal thoughts of girls and boys who attempted suicide both in the post-test and follow-up stages.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75540326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Prevalence of Anabolic Steroid Misuse, as Well as the Awareness Level and Attitude toward its Negative Effects among the Male Bodybuilder Athletes in Kermanshah, Iran, in 2019","authors":"Mehry Hoseini, B. Yousefi, Ali Ashraf Khazaei","doi":"10.52547/sjimu.29.5.22","DOIUrl":"https://doi.org/10.52547/sjimu.29.5.22","url":null,"abstract":"Article type: Orginal article Introduction: There have been several reports showing that the rate of anabolic-androgenic steroid abuse has increased in athlete and nonathlete adolescents. Therefore, this study aimed to assess the prevalence of anabolic steroid misuse, as well as the awareness level and attitude toward its negative effects on male bodybuilder athletes in Kermanshah, Iran. Material & Methods: This descriptive cross-sectional study was conducted in 2019. The statistical population included all male bodybuilder athletes in Kermanshah, Iran. The data were collected using a 25item self-reported anabolic-androgenic steroids questionnaire (demographic characteristics: n=7, awareness level: n=9, attitudes: n=5, and the prevalence of abuse: n=5) which was distributed using the multistage clustering method among 250 male bodybuilder athletes in Kermanshah, Iran. The obtained data were analyzed in SPSS software (version 22) through descriptive statistics (frequency, percentage, mean±SD) and inferential statistics (Pearson correlation coefficient). A p-value less than 0.05 was considered statistically significant. Findings: The results indicated that anabolic-androgenic steroid use was prevalent in 65.2% of the subjects. The most common cause was to increase muscle mass and strength. According to the results of this study, the prevalence of androgenic-androgenic steroid use showed a significant inverse relationship with attitude (r=-0.78; P=0.021), awareness (r=-0.73; P=0.032), an education level (r=-0.79; P=0.022). Moreover, sports background correlated significantly with the prevalence of androgenicandrogenic steroid use (r=0.68; P=0.035), attitude (r=0.84; P=0.013), and awareness (r=0.85; P=0.012). Discussion & Conclusion: The findings of this study indicated the high dose of anabolic-androgenic steroid use; moreover, the awareness level of the athletes was low in this regard. The low level of awareness, false attitude, and access to these steroids can lead to an increase in the use of them among athletes and different populations. Accordingly, health care professionals and sports specialists should consider these issues in the development of prevention plans and programs.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91088135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge and Compliance with Standard Precautions and its Predictors among Health Care Workers in Teaching Hospitals, Ilam, Iran","authors":"Zeinab Saraei, A. Azadi","doi":"10.52547/sjimu.29.3.1","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.1","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72801236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}