Journal of General Virology最新文献

The novel human astrovirus VA1 requires the proteasome during cell entry. 新型人类星状病毒VA1在进入细胞时需要蛋白酶体。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-10-01 DOI: 10.1099/jgv.0.002163

Luis E Jiménez, Susana López, Carlos F Arias, Tomás López

引用次数: 0

Discovery of a new konkovirus species in Lachenalia plants reveals possible co-evolution between 5' and 3' RNA sequence motifs. 在拉氏属植物中发现了一种孔氏病毒新种，揭示了5′和3′RNA序列基序可能的共同进化。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-10-01 DOI: 10.1099/jgv.0.002159

Rob J Dekker, Wim C de Leeuw, Marina F van Olst, Wim A Ensink, Selina M van Leeuwen, Timo M Breit, Martijs J Jonker

{"title":"Discovery of a new konkovirus species in Lachenalia plants reveals possible co-evolution between 5' and 3' RNA sequence motifs.","authors":"Rob J Dekker, Wim C de Leeuw, Marina F van Olst, Wim A Ensink, Selina M van Leeuwen, Timo M Breit, Martijs J Jonker","doi":"10.1099/jgv.0.002159","DOIUrl":"https://doi.org/10.1099/jgv.0.002159","url":null,"abstract":"This study reports the discovery of a new konkovirus species, named Lachenalia konkovirus 1 (LaKoV1), from Lachenalia plants in an urban botanic garden in Amsterdam. Using a combination of RNA sequencing (RNA-seq), small RNA-seq and advanced bioinformatics, we identified a segmented, negative-strand RNA virus belonging to the family Konkoviridae. Our findings show significant divergence between this novel virus and known members of the family Konkoviridae, such as tulip streak virus (TuSV) and Lactuca big vein-associated Phlebovirus (LBVaPV), supporting its classification as a distinct species. Notably, the sequence differences found in the conserved 5' and 3' ends of these segments suggest potential co-evolution. Despite the observed genomic distances, there is significant conservation in the RNA-dependent RNA polymerase subdomain, underscoring evolutionary relationships among LaKoV1, TuSV and LBVaPV. Our findings expand the known global virome and highlight the importance of exploring plant viromes in diverse ecological settings to better understand virus evolution and diversity.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and passaging of reptarenaviruses utilizing cultured snake cells suggest tissue tropism and restrictions in segment reassortment. 利用培养的蛇细胞分离和传代repreparenavirus，表明其具有组织亲和性和片段重组的限制性。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-10-01 DOI: 10.1099/jgv.0.002154

Annika Lintala, Udo Hetzel, Leonora Szirovicza, Emilia Timin, Anja Kipar, Jussi Hepojoki

{"title":"Isolation and passaging of reptarenaviruses utilizing cultured snake cells suggest tissue tropism and restrictions in segment reassortment.","authors":"Annika Lintala, Udo Hetzel, Leonora Szirovicza, Emilia Timin, Anja Kipar, Jussi Hepojoki","doi":"10.1099/jgv.0.002154","DOIUrl":"10.1099/jgv.0.002154","url":null,"abstract":"Reptarenaviruses cause boid inclusion body disease that can affect the fitness of the infected animals through a variety of clinical signs. Reptarenaviruses infect most tissue types in the affected individuals and spread efficiently in captive snake collections. Their genome consists of a small (S) and a large (L) segment, and the reptarenavirus-infected snakes often carry multiple genetically divergent reptarenavirus S and L segments, suggesting reptarenavirus coinfections occur frequently. We previously observed that reptarenavirus S and L segment combinations may vary between the tissues of an infected snake, leading to the hypothesis that the segment combination might contribute to tissue and/or species tropism. To test the hypothesis, we inoculated various cell lines derived from different tissues of several constrictor snake species with two samples containing multiple reptarenavirus segments (F15, two S and seven L segments; F17, one S and four L segments). We blind-passaged both virus samples five times in each cell line and monitored the presence of the segments in the supernatants through reverse transcription PCR. We also passaged the cells following the first inoculation with F17 and studied the segments present as above. The analysis revealed that some L segments were only present in supernatants with a specific S segment, suggesting preferred S and L segment pairs, thereby arguing against free reassortment of the segments. The results also showed that boa constrictor-derived cell lines supported reptarenavirus infection slightly better than pythonid-derived cell lines.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity of a trivalent haemorrhagic fever vaccine candidate against Sudan virus, Marburg virus and Lassa virus in an mpox vaccine. 一种三价出血热候选疫苗对麻疹疫苗中苏丹病毒、马尔堡病毒和拉沙病毒的免疫原性

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-10-01 DOI: 10.1099/jgv.0.002157

Martina Pfranger, Nina Krause, Benedikt Asbach, Johannes Meier, George Carnell, Lara Scheer, Anja Kalender, David Brenner, Paul Tonks, Simon Frost, Edward Wright, Ingo Jordan, Emma Kennedy, Roger Hewson, Barbara Blacklaws, Andrew Chan, Srivatsan Parthasarathy, Stuart Dowall, Miles Carroll, Volker Sandig, Sofiya Fedosyuk, Rebecca Kinsley, Jonathan Heeney, Ralf Wagner

{"title":"Immunogenicity of a trivalent haemorrhagic fever vaccine candidate against Sudan virus, Marburg virus and Lassa virus in an mpox vaccine.","authors":"Martina Pfranger, Nina Krause, Benedikt Asbach, Johannes Meier, George Carnell, Lara Scheer, Anja Kalender, David Brenner, Paul Tonks, Simon Frost, Edward Wright, Ingo Jordan, Emma Kennedy, Roger Hewson, Barbara Blacklaws, Andrew Chan, Srivatsan Parthasarathy, Stuart Dowall, Miles Carroll, Volker Sandig, Sofiya Fedosyuk, Rebecca Kinsley, Jonathan Heeney, Ralf Wagner","doi":"10.1099/jgv.0.002157","DOIUrl":"10.1099/jgv.0.002157","url":null,"abstract":"A multivalent vaccine targeting high-consequence infectious diseases in Sub-Saharan Africa (SSA), which are linked to high mortality, morbidity and overlapping clinical manifestations, would significantly improve health security and economic stability in this region. Trivalent vector vaccines were devised to deliver digitally optimized versions of Orthoebolavirus, Orthomarburgvirus glycoproteins (GPs) and a Lassa mammarenavirus (LASV) nucleoprotein (NP) by a single Modified Vaccinia Ankara (MVA) known to protect against mpox virus (MPXV) along with a matched DNA vaccine. Three immunizations in mice and Hartley guinea pigs with MVA only or a DNA prime followed by two MVA administrations induced comparable levels of binding antibodies and LASV-specific T-cells, respectively. While DNA priming mitigated MVA-specific antibody responses, GP- and NP-specific antibodies developed already after a single MVA vaccination. Although a post-outbreak Ebola virus vaccine is available, outbreaks by other filoviruses, annual LASV epidemics and increased incidence of MPXV infections support the rationale for an MVA-based trivalent haemorrhagic fever vaccine for endemic and high-risk human populations in SSA.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-dependent impairment of antibody responses to tick-borne encephalitis virus vaccination and infection in obese mice. 肥胖小鼠对蜱传脑炎病毒疫苗接种和感染的抗体反应的性别依赖性损伤。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-10-01 DOI: 10.1099/jgv.0.002161

Michal Dvorak, Dominik Arbon, Jiri Salat, Andrea Fortova, David Pajuelo Reguera, Tereza Frckova, Jiri Holoubek, Jana Balounova, Jan Prochazka, Radislav Sedlacek, Daniel Ruzek

{"title":"Sex-dependent impairment of antibody responses to tick-borne encephalitis virus vaccination and infection in obese mice.","authors":"Michal Dvorak, Dominik Arbon, Jiri Salat, Andrea Fortova, David Pajuelo Reguera, Tereza Frckova, Jiri Holoubek, Jana Balounova, Jan Prochazka, Radislav Sedlacek, Daniel Ruzek","doi":"10.1099/jgv.0.002161","DOIUrl":"10.1099/jgv.0.002161","url":null,"abstract":"Obesity is a growing global health concern with profound effects on immune function and vaccine efficacy. This study investigated the impact of obesity on immune responses to tick-borne encephalitis virus (TBEV) vaccination and infection using a mouse model. Mice on a high-fat diet (HFD) exhibited increased body weight, fat mass and a pre-diabetic state compared to standard chow diet (SCD) controls. After vaccination with the TBEV vaccine (Encepur), HFD mice showed significantly lower TBEV-specific IgG litres and neutralizing antibody levels compared to SCD mice. Splenocyte counts per organ mass were significantly higher in vaccinated SCD mice compared to their HFD counterparts, correlating with the elevated IgG litres observed in the SCD group. These results underscore the critical role of diet in shaping the immune response and vaccine efficacy. Following TBEV infection, HFD mice did not display increased disease severity or elevated viral litres in the serum, spleen or brain relative to SCD controls, indicating that obesity did not exacerbate viral replication or dissemination. However, a sex-dependent effect of obesity on the humoral immune response was observed. Male HFD mice produced antibody litres comparable to their SCD counterparts, suggesting minimal impact of obesity on their immune response. In contrast, female HFD mice exhibited significant impairments in TBEV-specific IgG and neutralizing antibody production compared to female SCD mice, as well as both male HFD and male SCD groups. These findings highlight a complex interplay between obesity, sex and immune function, with obesity disproportionately impairing the immune response after TBEV vaccination and infection.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. PUM2结合SARS-CoV-2 RNA和PUM1轻度降低病毒RNA水平，但这两种蛋白都不影响子代病毒的产生。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-09-01 DOI: 10.1099/jgv.0.002152

Nhi Phan, Yelizaveta Zaytseva, Chia-Ching Lin, Mitali Mishra, Weina Sun, Paulina Pawlica

{"title":"PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production.","authors":"Nhi Phan, Yelizaveta Zaytseva, Chia-Ching Lin, Mitali Mishra, Weina Sun, Paulina Pawlica","doi":"10.1099/jgv.0.002152","DOIUrl":"10.1099/jgv.0.002152","url":null,"abstract":"Pumilio proteins (PUM1 and PUM2) are essential post-transcriptional regulators of gene expression found across plants, animals and yeast. They bind Pumilio response elements (PREs) on messenger RNAs (mRNAs) to modulate mRNA stability and translation. PUMs have been implicated in diverse cellular processes, including stem cell maintenance, neurogenesis and cell cycle regulation. They have also been reported to negatively regulate innate immunity genes and to participate in viral RNA sensing. Previous high-throughput interactome studies revealed that PUMs bind severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. We found that SARS-CoV-2 transcripts contain multiple conserved PREs, some of which are preferentially bound by PUM2. Surprisingly, altering PUM levels does not affect the production of progeny virions. However, depletion of PUM1 slightly increases intracellular viral RNA levels, suggesting that PUM1 either plays a mild antiviral role against SARS-CoV-2 or regulates host factors that promote viral replication. Notably, PUM1 also negatively regulates innate immunity gene expression both at steady state and during SARS-CoV-2 infection. Our findings support a complex immunomodulatory role for PUM1, acting both as a negative regulator of innate immunity genes and a mild inhibitor of SARS-CoV-2 RNA accumulation. However, in cell culture, these roles appear negligible based on viral progeny output. Whether the multiple PREs found in the SARS-CoV-2 genome contribute to evasion of PUM1 activity remains an open question.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 9","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Susceptibility of different mouse strains to SARS-CoV-2 spike receptor-binding domain protein-induced lung inflammation: a comparative study. 不同小鼠品系对SARS-CoV-2刺突受体结合域蛋白诱导的肺部炎症易感性的比较研究

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-09-01 DOI: 10.1099/jgv.0.002148

Yujeong Ha, Young Hyun Lee, Hyo-Sung Jo, Tae Woo Kwon, Yujin Seo, Dong Woon Kim, Ik-Hyun Cho

{"title":"Susceptibility of different mouse strains to SARS-CoV-2 spike receptor-binding domain protein-induced lung inflammation: a comparative study.","authors":"Yujeong Ha, Young Hyun Lee, Hyo-Sung Jo, Tae Woo Kwon, Yujin Seo, Dong Woon Kim, Ik-Hyun Cho","doi":"10.1099/jgv.0.002148","DOIUrl":"10.1099/jgv.0.002148","url":null,"abstract":"Transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) are widely used in research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to their susceptibility to viral infection. However, the extent to which genetic differences among mouse strains affect inflammatory responses to the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein remains unclear. In this study, we compared the susceptibility of five commonly used mouse strains - DBA2, ICR, C3H/HeOuJ, BALB/c and C57BL/6J - to RBD-induced pulmonary inflammation. Histopathological analysis revealed that DBA2 and C57BL/6J mice exhibited significantly heightened inflammatory responses, characterized by increased angiotensin-converting enzyme 2 (ACE2) expression, macrophage infiltration, upregulation of proinflammatory cytokines (e.g. IL-1β, TNF-α) and activation of the IL-6/STAT3 and nicotinamide adenine dinucleotide phosphate oxidase pathways. These responses were markedly attenuated by pretreatment with an anti-ACE2 antibody, supporting a potential role of RBD-ACE2 interactions in driving inflammation, although ACE2-independent mechanisms cannot be excluded. Our findings suggest that DBA2 and C57BL/6J mice are particularly sensitive to RBD exposure and represent cost-effective and physiologically relevant models for studying ACE2-mediated lung inflammation and for evaluating therapeutic interventions targeting coronavirus disease 2019-related inflammatory mechanisms.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 9","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ICTV Virus Taxonomy Profile: Duplodnaviria 2025. ICTV病毒分类概况：双星病毒2025。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-09-01 DOI: 10.1099/jgv.0.002139

Evelien M Adriaenssens, Ryan Cook, Valerian Dolja, Eugene V Koonin, Mart Krupovic, Jens H Kuhn, Cédric Lood, Alejandro Reyes Muñoz, Dann Turner, Arvind Varsani, Paola K Vaz, Thomas Waltzek, Yuri I Wolf, Natalya Yutin, F Murilo Zerbini

引用次数: 0

Investigating factors driving shifts in subtype dominance within H5Nx clade 2.3.4.4b high pathogenicity avian influenza viruses. 研究H5Nx分支2.3.4.4b高致病性禽流感病毒亚型优势变化的驱动因素。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-09-01 DOI: 10.1099/jgv.0.002150

Elizabeth Billington, Cecilia Di Genova, Caroline J Warren, Saumya S Thomas, Simon Johnson, Sofia Riccio, Dilhani De Silva, Jacob Peers-Dent, Nigel Temperton, Kelly da Costa, Alexander M P Byrne, Maisie Airey, Audra-Lynne Schlachter, Jiayun Yang, Alejandro Nunez, Munir Iqbal, Marek J Slomka, Ian H Brown, Ashley C Banyard, Joe James

{"title":"Investigating factors driving shifts in subtype dominance within H5Nx clade 2.3.4.4b high pathogenicity avian influenza viruses.","authors":"Elizabeth Billington, Cecilia Di Genova, Caroline J Warren, Saumya S Thomas, Simon Johnson, Sofia Riccio, Dilhani De Silva, Jacob Peers-Dent, Nigel Temperton, Kelly da Costa, Alexander M P Byrne, Maisie Airey, Audra-Lynne Schlachter, Jiayun Yang, Alejandro Nunez, Munir Iqbal, Marek J Slomka, Ian H Brown, Ashley C Banyard, Joe James","doi":"10.1099/jgv.0.002150","DOIUrl":"https://doi.org/10.1099/jgv.0.002150","url":null,"abstract":"H5Nx clade 2.3.4.4b high pathogenicity avian influenza viruses (HPAIVs) have decimated wild bird and poultry populations globally since the autumn of 2020. In the UK and in continental Europe, the H5N8 subtype predominated during the first epizootic wave of 2020/21, with few detections of H5N1. However, during the second (2021/22) and third (2022/23) epizootic waves, H5N1 was the dominant subtype. The rapid shift in dominance from H5N8 to H5N1 was likely driven by a combination of virological, immunological and/or host-related factors. In this study, we compared viral fitness and immunological responses in ducks, a key reservoir species, using dominant genotypes of H5N1 (genotype AB) and H5N8 (genotype A) from the second wave. While viral shedding dynamics were similar for both viruses, H5N8 was more pathogenic. Antigenic analysis of post-infection duck sera revealed that the haemagglutinin protein was antigenically similar across clade 2.3.4.4b H5 HPAIVs, but neuraminidase proteins displayed different patterns of cross-reactivity. We also modelled a scenario where ducks were pre-exposed to H5N1 (genotype C) or H5N8 (genotype A) from the first wave and subsequently challenged with either homologous or heterologous subtypes from the second wave (genotype AB or A). Despite the absence of seroconversion, pre-exposure to different subtypes resulted in varying clinical outcomes following challenge. These findings indicate that both viral and immunological factors likely played significant roles in the emergence and spread of H5Nx HPAIVs in wild bird populations.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 9","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Autographa californica multiple nucleopolyhedrovirus-encoded microRNA, AcMNPV-miR-5, downregulates the expression of viral gene ac66. 加州签名虫多核多角体病毒编码的microRNA AcMNPV-miR-5下调病毒基因ac66的表达。

IF 4.3 4区医学

Journal of General Virology Pub Date : 2025-09-01 DOI: 10.1099/jgv.0.002155

Tingkai Teng, Zhuowen Duan, Ang Li, Jinwen Wang

{"title":"An Autographa californica multiple nucleopolyhedrovirus-encoded microRNA, AcMNPV-miR-5, downregulates the expression of viral gene ac66.","authors":"Tingkai Teng, Zhuowen Duan, Ang Li, Jinwen Wang","doi":"10.1099/jgv.0.002155","DOIUrl":"10.1099/jgv.0.002155","url":null,"abstract":"Four Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-encoded microRNAs (miRNAs) have been characterized previously. Here, we report the fifth AcMNPV-encoded miRNA, AcMNPV-miR-5 (Ac-miR-5), which downregulates the viral gene ac66. Target genes were predicted through sequence analysis and validated using luciferase reporter assays. The regulatory effects of Ac-miR-5 on ac66 expression were assessed by reverse transcription quantitative PCR and Western blot. The impact of Ac-miR-5 overexpression on virus infection was analysed by TCID50 assay and quantitative real-time PCR in Sf9 cells. The results showed that Ac-miR-5 downregulates ac66 at both the mRNA and protein levels. Meanwhile, the budded virion production and DNA replication were decreased. Furthermore, microscopy revealed a decrease in the number of polyhedra formed. These findings suggest that Ac-miR-5 overexpression restricts viral load, potentially contributing to the establishment of a stable viral infection within the host cell.","PeriodicalId":15880,"journal":{"name":"Journal of General Virology","volume":"106 9","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0