Journal of Experimental & Clinical Medicine最新文献

{"title":"Evaluation of metabolic and hormonal parameters in women with PCOS living in Black Sea Region","authors":"N. Dokuzeylül Güngör, S. Çelik, M. Güçlü, S. Çelik, F. Cengiz","doi":"10.52142/OMUJECM.38.3.5","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.5","url":null,"abstract":"Polycystic ovary syndrome(PCOS) is the most common endocrinopathy which is frequently associated with metabolic syndrome. The aim of this study is to compare the clinical,biochemical and hormonal characteristics of healthy women and women with PCOS in Black Sea Region.91 healthy women(Group=1) and 109 PCOS(Group=2) patients were included in this retrospective study. This study was conducted in Samsun Women and Children’s Health Research and Training Hospital between October 2019-May 2020.The anthropometric, clinical and laboratory characteristics of the women were recorded. The Institutional Review Board of the hospital approved the study. IBM SPSS Statistics 22 programme was used for statistical analysis. . Variables were expressed as mean ± standard deviation or as a number (percentage), and statistical significance was defined as a p value of less than 0.05.In this study, no statistically significant difference was found between patients with PCOS and control subjects in age,height ,BMI, waist circumference and waist-to-hip ratio. Weight ,BP systolic,BP diastolic and FG scores, were significantly higher in patients with PCOS compared to control subjects.In this study,no statistically significant difference was found between patients with PCOS and control subjects in FSH, estradiol prolactin ,TSH,fT3,fT4 and DHEASO4 levels.But,LH, cortisol, total and free testesterone and 17-OH progesteron levels were significantly higher in patients with PCOS compared to control subjects.Also in this study, vitamin B12 and the 25-OH-D levels were significantly lower in patients with PCOS compared to control subjects.HbA1C,fasting blood glucose levels,fasting insulin levels and HOMA-IR were significantly higher in patients with PCOS compared to control subjects . Total-C , LDL-C and TG levels were significantly higher in women with PCOS compared to control subjects.Also HDL-C levels were significantly lower in women with PCOS compared to control subjects.As a result, the risk of metabolic syndrome is increased in PCOS patients because of high androgen levels,obesity and insulin resistance.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"36 1","pages":"235-240"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75368580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonsynonymous variations of ion channel-related genes as risk factors in epilepsy","authors":"Burcu BİTERGE SÜT, Hayriye Soytürk","doi":"10.52142/OMUJECM.38.3.15","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.15","url":null,"abstract":"Recurrent seizures are characteristic to epilepsy, which often arise due to increased electrical activity. Ligand-gated ion channels are considered as key factors in epilepsy as they regulate and maintain neuronal membrane potential via regulating ion transportation. Therefore, this study aims to identify ion channel-related single nucleotide variations that are considered as risk factors in epilepsy and determine their potential effects on pathogenicity, protein stability and structure using in silico methods. For this purpose, ion channel-related mutations linked with epilepsy were retrieved from ClinVar. Pathogenicity scores and protein stability were predicted using FATHMM-XF and MUpro, respectively. Structural alterations were determined via HOPE server. We identified 17 epilepsy-related missense mutations, 11 of which were in ion channel-related genes. Nonsynonymous substitutions of p.E177A, p.D219N, p.A322D, p.R577Q, p.E282K, p.V831M and p.R1072C were determined as pathogenic, while all mutations resulted in varying degrees of decrease in overall protein stability. Furthermore, all variants were annotated with risk for disease and introduction of distinct side chains caused differences in size, charge and hydrophobicity, as well as contact with other proteins and ligands. In conclusion, mutations in ion channel-related genes were previously identified in several genetic association studies while their functional annotations were not addressed. The results of this study provide a functional explanation to the pathogenic effects of ion channel-related gene mutations that are considered as risk factors in epilepsy.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"9 1","pages":"288-293"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74137597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell line: A model system to understand Type 2 Diabetes Mellitus","authors":"S. Ningsih, Rizkyana Avissa, E. Stujanna, Erlin Listyaningsih, T. Yashiro, Wawang Setiawan n SUKARYA","doi":"10.52142/OMUJECM.38.3.1","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.1","url":null,"abstract":"Type 2 Diabetes Mellitus (T2DM) is one of the major health issues in the world. The cellular mechanism of T2DM is still not fully understood. It could be studied by using spheroid three-dimensional (3D) culture which is considered representative of the in vivo conditions. Several types of pancreatic β cell lines have been used, one of which is the insulin-GLase (iGL) cell line. This study aims to evaluate the effect of cell density and incubation time on spheroid morphology and cell viability in order to understand which one can be considered as the best option in studying T2DM using iGL cell. Spheroid was made by using the Hanging drop method. The variations of initial seeding cells were 50, 100, 200, and 400 cells/µL then incubated for 1, 2, 3, and 4 days. The evaluated parameters in this study are spheroid morphology and cell viability. Spheroid morphology was observed by using inverted phase contrast microscope integrated with camera (Nikon) and NIS-Elements Analysis D software. Cell viability was determined by using LUNA-II™ Automated Cell Counter (Logos Biosystem). The result of this study showed that spheroid in all of the group cell concentration have formed since the first day and its diameter was significantly increased on the following days (p<0,05). The spheroid size was positively correlated with the cell density in group 50-200 cells/µL. A single and stable spheroid morphology was observed in 50-100 cells/µL group. Cell viability in 3D culture system was lower and significantly decreased since day 3 compared to 2D culture (p <0.05; 0.01). In conclusion, spheroid derived from iGL cell line with a stable morphology and good viability could be obtained from a cell concentration of 50-100 cells / µL with two days of incubation.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"29 1","pages":"211-215"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81670389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of myths about sexuality and the level of knowledge about sexuality on the marital satisfaction in married couples","authors":"Birgül EMIROGLU BAKAY, Eylem AYRANCI ORHON, Kadir Bakay, Faruk Olcenoglu, D. Guven, I. Yalcin, Merve Uyar","doi":"10.52142/OMUJECM.38.3.26","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.26","url":null,"abstract":"Objective: To look into the effect of sexual myths and level of knowledge about sexuality on marital satisfaction in married couples. Methods: The study was carried on with 104 voluntary respondents; 57 of which are married women and 47 are married men. The data has been collected with Personal Information Questionnaire, Marital Adjustment Test, Sexual Myths Analysis Questionnaire and The Golombok Rust Inventory of Sexual Satisfaction (GRISS), data has been analysed with SPSS 15.0 software package (Statistical Package for Social Sciences). Results and Conclusion: According to these findings, as the belief in sexual myths increases, the sexual satisfaction decreases; yet the increase in the belief in sexual myths does not affect the marital satisfaction. The findings implicate that as the knowledge about sexuality increases, the sexual satisfaction increases while the marital satisfaction decreases. As the number of sexual myths increases, marital adjustment decreases. The findings don’t show any significant correlation between sexual satisfaction and marital adjustment","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"30 1","pages":"340-344"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81620904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can histopathology of gastric tissue resected in sleeve gastrectomy be informative about serum iron levels?","authors":"Y. Adalı, Özge Ertener, Hatice Beşeren, Kenan Binnetoğlu","doi":"10.52142/OMUJECM.38.3.21","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.21","url":null,"abstract":"Aim: One of the most common post-operative deficiencies after bariatric surgery is iron deficiency and one of the important determinants of post-operative iron deficiency is the preoperative condition. In this study, it was aimed to investigate the relationship between iron levels and histopathological findings observed in gastric tissue resected in sleeve gastrectomy. Materials and methods: Preoperative and postoperative iron levels were compared with the presence of inflammation, atrophy, Helicobacter pylori, intestinal metaplasia, lymphoid follicles and lymphoid aggregates observed in patients operated due to morbid obesity. Findings: The postoperative serum iron levels and preoperative values were compared and a statistically significant increase was found due to the use of iron-containing preparations after the operation. Among the parameters evaluated, inflammation, atrophy, Helicobacter pylori, intestinal metaplasia, and the presence of lymphoid follicles were not found to be associated with iron levels, but it was noted that the presence of lymphoid aggregate in all cases and male cases was correlated with preoperative low iron levels (p values 0.047 and 0.015 respectively). Conclusion: In this study, which investigated the role of histopathological findings in the prediction of iron deficiency in sleeve gastrectomies, the relationship between preoperative iron levels, which was reported to be predictive for post-operative iron deficiency, and the presence of lymphoid aggregates was revealed. It is thought that other histopathological findings such as the presence of lymphoid follicle and Helicobacter pylori are also important in terms of iron levels but could not be revealed due to the limitations of the study.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"2 1","pages":"318-321"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87104184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the presence of AmpC (FOX) beta-lactamase gene in clinical strains of Escherichia coli isolated from hospitalized patients in Tabriz, Iran","authors":"Z. Sadeghi-Deylamdeh, Abolfazl Jafari-Sales","doi":"10.52142/OMUJECM.38.3.17","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.17","url":null,"abstract":"Background and purpose: Beta-lactamase enzymes inactivate beta-lactam antibiotics by hydrolyzing the central nucleus. AmpC-type beta-lactamases hydrolyze cephalosporins, penicillins, and cephamycins. Therefore, the aim of this study was to determine antibiotic resistance and to investigate the presence of AmpC beta-lactamase gene in clinical strains of Escherichia coli isolated from isolated from hospitalized patients in Tabriz. Materials and Methods: In this cross-sectional descriptive study, 289 E. coli specimens were collected from clinical specimens. Disk diffusion method and combined disk method were used to determine the phenotype of extended spectrum β-Lactamase producing (ESBLs) strains. Then PCR was used to evaluate the presence of AmpC (FOX) beta-lactamase gene in the strains confirmed in phenotypic tests. Antibiotic resistance was also determined using disk diffusion by the Kibry-Bauer method. Results: A total of 121 isolates were identified as generators of beta-lactamase genes. 72 (59.5 %) isolates producing ESBL and 49 (40.5 %) isolates were identified as AmpC generators. In the PCR test, 31 isolates contained the FOX gene. The highest resistance was related to the antibiotics amoxicillin (76.12%), ceftazidime (70.24%) and nalidixic acid (65.05%). Conclusion: The results indicate an increase in the prevalence of beta-lactamase genes and increased resistance to beta-lactam antibiotics, which can be the result of improper use of antibiotics and not using antibiotic susceptibility tests before starting treatment. Also, using phenotypic and molecular diagnostic methods such as PCR together can be very useful.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"358 1","pages":"301-304"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74979176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the association between bilateral calcaneal fractures and suicide attempts: Findings from 4 different trauma centers in Turkey","authors":"Emre Gültaç, A. Açan, C. Kilinç, N. Gündüz, N. D. Demirkıran, Mehmet Yücens, N. Aydogan","doi":"10.52142/OMUJECM.38.3.3","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.3","url":null,"abstract":"Background: We aimed to to determine the association between bilateral calcaneal fractures and suicide attempt and some socio-demographic characteristics. Methods: This study was conducted among patients admitted to the emergency departments of four trauma centers with bilateral calcaneal fractures between January 2014 and March 2017. The Sociodemographic Data Form, Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders fourth edition (DSM-IV) and Axis I Disorders (SCID-I) were applied to participants. Comparisons about age and gender was made between suicidal and non-suicidal patiens with bilateral calcaneal fractures. Results: Twenty-three patients with bilateral calcaneus fractures were included in the study. Of the 23 patients with bilateral calcaneal fractures, 14 (61%) were evaluated as suicidal and 9 (39%) were evaluated as not suicidal. The majority of suicidal jumpers were female (n = 11, 78.6%). Suicidal patients were younger compared with non-suicidal fallers. As a result of SCID I interview, 9 (64.3%) of 14 suicidal jumpers were diagnosed with serious psychiatric diagnoses: One patient was diagnosed with first episode psychotic disorder, one with schizophrenia, one with Schizoaffective Disorder, one with Bipolar Disorder, two with Major Depressive Disorder and three with Adjustment Disorder. Conclusion: Based on our study results we determined that suicidal jumpers are frequently diagnosed with serious psychiatric disorders. Therefore, the psychiatric dimension should be taken into consideration in the treatment plan for patients with bilateral calcaneal fractures and the multidisciplinary approach should be taken.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"5 1","pages":"221-226"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72748641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reverse shoulder arthroplasty-early results","authors":"F. Say, Numan Kuyubaşi, A. Pişkin, M. Bülbül","doi":"10.52142/OMUJECM.38.3.2","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.3.2","url":null,"abstract":"Although reverse shoulder arthroplasty is used in massive rotator cuff tears, it is also used in proximal humerus fractures and shoulder arthroplasty revision. In this study, we aimed to examine the early radiological and clinical results of patients undergoing reverse shoulder arthroplasty with different diagnoses. Between 2010 and 2013, reverse shoulder arthroplasty was applied to 10 patients (4 men, 6 women) with the diagnosis of arthropathy due to rotator cuff tear (n: 7), multi-fragmentary proximal humerus fracture (n: 2) and hemiarthroplasty revision (n: 1). The median age of the patients was 74 (64- 85) years and the median follow-up was 15.5 (3-35) months. Patients were assessed in terms of joint range of motion, Constant score and Visual Pain Scale (VAS) and radiological examinations before and after surgery. Preoperative active shoulder flexion, abduction, internal and external rotation degrees of the patients were 65, 30, 40 and 50 degrees, and 105, 95, 30, 57.5 degrees respectively in their final controls. Constant score was 20.5 (14-63) preoperatively, and 54.5 (38-64) in the final controls (p˂0.05). While the preoperative VAS was 7 (3-9), it was found to be 1.5 (1-3) in the final controls (p˂0.05). As a complication, scapular notching was observed in one patient. Internal- external rotation values were better in the rotator cuff arthropathy group than the fracture group. Reverse shoulder arthroplasty, especially in the treatment of patients with rotator cuff arthropathy, has good early results and a low complication rate. We recommend paying attention to the glenoid component location to avoid scapular notching.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"95 1","pages":"216-220"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81873170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of toxoplasmosis risk on anxiety and perception of cat owner pregnant women","authors":"N. Güngör, T. Gürbüz, S. Zincir, B. Dokuzeylül, E. Or, F. Cengiz","doi":"10.52142/OMUJECM.38.2.18","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.2.18","url":null,"abstract":"Abstract Purpose: The aim of this study is to evaluate toxoplasmosis risk on anxiety and perception of cat owner pregnant women Method: A Health Anxiety Inventory (HAI-18) was used to measure the anxiety of participants. Age and anxiety variables were analyzed using a two-sample independent t-test. Gravida, education, and job status were analyzed using chi-square tests, and Live to abort ratio & pregnancy number were analyzed using Fisher exact test. Results: Measurement of the anxiety level between the two groups showed that anxiety is significantly higher among the pregnant women who feed cats in their house with an average of 32.03(±8.72). The average anxiety level among the pregnant women who don't have cats in their house was 25.94 (±8.99). The difference between the Health Anxiety Inventory of the two groups was significant (P-Value: 0.0001) Conclusion: Our results showed that pregnant women who own cats at home had, on average, more environmental anxiety than women who don’t feed cats at home.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"2006 1","pages":"154-158"},"PeriodicalIF":0.0,"publicationDate":"2021-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86974341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic And Therapeutic Approaches For Non-Variceal Upper Gastrointestinal Bleeding","authors":"T. Ayyildiz, M. Duygulu","doi":"10.52142/OMUJECM.38.SI.GASTRO.1","DOIUrl":"https://doi.org/10.52142/OMUJECM.38.SI.GASTRO.1","url":null,"abstract":"Upper gastrointestinal bleeding (UGIB) is a common, life-threatening medical condition. Non-variceal causes account for more than 90% of bleeding episodes. Peptic ulcer disease is the most frequent cause of non-variceal UGIB. Patients present with hematemesis and/or melena but hematochezia might be present in patients with severe bleeding. Despite advances in diagnostic and therapeutic methods, mortality remains high in the elderly and patients with comorbidities. Endoscopy is the primary procedure that should be performed to identify the etiology of UGIB and for treatment purposes following adequate resuscitation of patients. Early endoscopy (within the first 24 hours) has considerably improved the clinical outcomes. A number of scoring systems are being used in patients with UGIB to identify the risk of complications, rebleeding risk, the need for interventional procedures and the risk of death. The most commonly used scoring tools are the Rockall score, Glasgow-Blatchford score and AIMS65 score. Primary treatment modalities include adequate resuscitation, intravenous fluid support, transfusion of red blood cell suspension, acid suppression therapy and administration of prokinetic agents. In general, angiography, computed tomography, technetium-99m-labeled red blood cell scintigraphy and capsule endoscopy may be used in patients whose bleeding cannot be detected endoscopically. Interventional radiological procedures should be initially performed for hemorrhages that cannot be stopped endoscopically and surgical options should be considered when interventional radiological procedures are out of reach or unsuccessful.","PeriodicalId":15770,"journal":{"name":"Journal of Experimental & Clinical Medicine","volume":"57 1","pages":"11-16"},"PeriodicalIF":0.0,"publicationDate":"2021-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79805284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}